Inactive Dry Yeast Research Articles

Evaluation of SO2, glutathione, and glutathione-rich inactive dry yeast as antioxidants on the fermentation properties of Roxburgh rose wine

Cili (Roxburgh rose) wine, which is rich in ascorbic acid, flavones, and polyphenols, presents unique challenges in wine production, including oxidation, aroma loss, and SO2 residues. This study investigated the effects of three antioxidants (SO2, glutathione (GSH), and GSH-rich inactive dry yeast (g-IDY)) on the fermentation properties of cili wine and evaluated the potential of g-IDY for addressing the aforementioned issues. g-IDY significantly enhanced the fermentation rate, as evidenced by increased CO2 production within 48 h, and maintained high levels of GSH, total phenolics, total flavones, and ascorbic acid, contributing to the antioxidant capacity and overall stability of the wine. g-IDY also improved the retention and concentration of key aromatic compounds, including higher alcohols, esters, and terpenes, thereby enhancing the sensory profile of the wine. Principal component analysis confirmed that different antioxidants significantly altered the volatile profile, with g-IDY demonstrating distinct improvements. The optimal concentration was 400 mg/L g-IDY. Compared with traditional antioxidants like SO2 and pure GSH, g-IDY offers a safer and more effective solution for preserving the quality of cili wine. This study provides experimental evidence for refining the fermentation process and promotes the use of g-IDY for enhancing the antioxidant capacity and sensory attributes of cili wine.

Different Nitrogen Consumption Patterns in Low Temperature Fermentations in the Wine Yeast Saccharomyces cerevisiae.

Nowadays, the wine industry carries out fermentations at low temperatures because this oenological practice clearly improves the aromatic complexity of the final wines. In addition, nitrogen content of the must also influences the quality of the wine. In this study, we carried out a phenotypic and fermentative analysis of two industrial wine Saccharomyces cerevisiae strains (P5 and P24) at 15 and 28 °C and three nitrogen concentrations (60, 140 and 300 mg N/L) in synthetic must. Our results show that both parameters, temperature and nitrogen, are interrelated and clearly determine the competitiveness of the wine strains and their ability to adapt at low temperatures. The best adapted strain at low temperatures decreased its competitiveness at lower nitrogen concentrations. In addition, our results show that it is not only the quantity of nitrogen transported that is important but also the quality of the nitrogen source used for wine yeast adaptation at low temperatures. The presence of some amino acids, such as arginine, branched chain amino acids, and some aromatic amino acids can improve the growth and fermentation activity of wine yeasts at low temperatures. These results allow us to better understand the basis of wine yeast adaptation to fermentation conditions, providing important information for winemakers to help them select the most appropriate yeast strain, thus reducing the economic costs associated with long and sluggish fermentations. The correlation between some amino acids and better yeast fermentation performance could be used in the future to design inactive dry yeast enriched in some of these amino acids, which could be added as a nutritional supplement during low temperature fermentations.

Study on the Kinetic Model of Mixed Fermentation by Adding Glutathione-Enriched Inactive Dry Yeast

In order to investigate the impact of glutathione-enriched inactive dry yeast (g-IDY) on the co-fermentation process of Torulaspora delbrueckii and Saccharomyces cerevisiae, different contents of g-IDY (0, 20, 40, and 100 mg/L) were added to the simulated liquid for fermentation. The yeast quantity, reducing sugar content, and ethanol volume fraction in the fermentation system were determined every 24 h. Nonlinear fitting of the measured values was carried out using classical Logistic, SGompertz, Boltzmann, and DoseResp models. Additionally, the aroma components of the wine were analyzed by GC-MS. The results indicate that the Logistic model performs best in terms of yeast growth kinetics, whereas the DoseResp and Boltzmann models exhibit the same fitting performance for reducing sugar consumption, both superior to the Logistic model, and the Boltzmann model shows the best-fitting performance for ethanol production. All optimal models have fitting coefficients (R2 values) above 0.99, demonstrating that different contents of g-IDY can effectively complete fermentation. Furthermore, all three fitting models can effectively describe the fermentation process using g-IDY. The use of g-IDY can increase the content of ethyl phenylacetate and phenylethanol, which can be employed to enhance the aroma of wine.

Effect of SO2, glutathione, and glutathione-rich inactive dry yeast on the color, phenolic compounds, ascorbic acid, and antioxidant activity of Roxburgh rose wine.

Conventional methods for inhibiting browning in wine are not suitable for cili (Roxburgh rose) wine, which is naturally rich in ascorbic acid and subject to restrictions on SO2 addition. In this study, the capacity of various additives to suppress the browning of cili wine caused by ascorbic acid degradation was investigated. SO2, pure reduced glutathione (GSH), regular inactive dry yeast (IDY), and IDY with various levels of glutathione enrichment (g-IDY) were separately introduced into cili wine following the completion of alcoholic fermentation. Over a period of 12 months, the color parameters, levels of ascorbic acid, phenolic compounds, antioxidant activity, and GSH content of the aged cili wine were analyzed. Among the investigated additives, g-IDY exhibited the strongest inhibitory effect on browning. Moreover, adding 800mgL-1 g-IDY increased the total reducing power and residual GSH content by factors of 1.52 and 2.44, respectively, with respect to those of the SO2-treated cili wine, thus enhancing its antioxidant capacity. Using ultra-performance liquid chromatography-tandem mass spectrometry analysis, a total of 22 monomeric phenolic compounds were identified. After g-IDY treatment, the contents of 15 easily oxidizable o-diphenols decreased, preventing the depletion of ascorbic acid as an antioxidant. As a result, the levels of ascorbic acid and total phenolics were 1.5-fold and 1.17-fold higher than those in the SO2-treated wine, respectively. This study demonstrates that g-IDY provides a new approach to preventing the browning of wine caused by ascorbic acid degradation. PRACTICAL APPLICATION: Cili wine, characterized by its high ascorbic acid content, can decelerate cellular senescence and bolster immune function, which has contributed to its popularity. Ascorbic acid, a potent antioxidant, can be spiked into white wines to significantly enhance their antioxidative properties. Nevertheless, the high ascorbic acid content in cili wine renders it susceptible to oxidation under both aerobic and anaerobic conditions, which alters the wine's hue from golden to dark brown, thus diminishing its commercial value. Overcoming this browning associated with ascorbic acid degradation is therefore of considerable importance and could facilitate the advancement of the cili industry.

Inactive dry yeast to improve the oxidative stability of Spanish dry-fermented sausage “salchichón”

The use of inactive dry yeasts (IDY) of Saccharomyces cerevisiae as a natural alternative to sodium ascorbate to inhibit the oxidation reactions of Spanish dry-fermented sausage “salchichón” was studied. Control sausages and those treated with different IDY concentrations were monitored and analyzed at 0, 7, 14, 21 and 28 d throughout the ripening process. The application of IDY (1.5 and 3 g/100 g) did not affect the physicochemical parameters and microbial counts over time. However, IDY addition achieved a more stable phenolic content, antioxidant activity of samples was increased, and a lower lipid and protein oxidation were observed compared to the use of sodium ascorbate. Likewise, sausages with 3 g/100 g IDY showed similar or lower quantities of volatile compounds related to lipid oxidation at the end of ripening than those elaborated with sodium ascorbate. From a sensory point of view, IDY addition provided bread/yeast and sweet notes to sausages. However, consumers did not find differences among samples in a hedonic ranking test. Therefore, IDY could be employed in “salchichón” as natural preservatives providing pleasant sensory attributes to the product.

Multivariate analysis reveals effect of glutathione-enriched inactive dry yeast on amino acids and volatile components of kiwi wine

Principal component analysis (PCA) and partial least squares (PLS) regression were applied to investigate the effect of glutathione-enriched inactive dry yeast (g-IDY) on the amino acids and volatile components of kiwi wine. Results indicated that the addition of g-IDY had positive effect on most amino acids of kiwi wine, especially glutamine and glycine. In case of pure juice fermentation, the concentrations of ethyl decanoate, 2-methylbutyric acid, trans-2-nonenal and hexyl butyrate had notably positive correlation with the addition of g-IDY. PLS regression indicated that the amino acids were highly interrelated to the volatile compositions, and glycine had the strongest positive impact on the concentrations of esters and total volatile components. This might explain the similar effect of g-IDY on the amino acids and volatile components of kiwi wine. Besides, PLS regression showed that E-nose was a good method to predict volatile compositions of kiwi wine, especially esters.

Impact of oenological antioxidant substances on the formation of 1-hydroxyethyl radical and phenolic composition in SO2 free red wines.

Different natural substances, chitosan, inactive dry yeasts and freeze-dried aqueous extracts from two wine industry by-products (stems and shoots) were used in red winemaking as possible alternatives to SO2 . The resistance to oxidation of wines was evaluated by electron paramagnetic resonance. The phenolic composition of wines was analyzed by high-performance liquid chromatography-diode array detection/electrospray ionization mass spectrometry, antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl radical) and ABTS [2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation] assays and spectrophotometric measurements of color were compared. The wines elaborated with chitosan and inactive dry yeast presented greater capacity to inhibit the formation of the 1-hydroxyethyl radical compared to the wines elaborated with stem or shoot extracts. The total content of anthocyanins was higher in the wines elaborated with SO2 ; however, the concentration of flavan-3-ols was higher in the wines with chitosan. In addition, the wines with chitosan and inactive dry yeast presented the highest % polymerization. Wines elaborated with stem extract had a lower concentration of flavonols and stilbenes. Chitosan and inactive dry yeast, which are used as an alternative to SO2 in winemaking, allow the control of the formation of 1-HER in red wines. Wines with stem and shoot extracts showed a lower resistance to oxidation. © 2020 Society of Chemical Industry.

Candida utilis yeast as a functional protein source for Atlantic salmon (Salmo salar L.): Local intestinal tissue and plasma proteome responses.

Microbial ingredients such as Candida utilis yeast are known to be functional protein sources with immunomodulating effects whereas soybean meal causes soybean meal-induced enteritis in the distal intestine of Atlantic salmon (Salmo salar L.). Inflammatory or immunomodulatory stimuli at the local level in the intestine may alter the plasma proteome profile of Atlantic salmon. These deviations can be helpful indicators for fish health and, therefore potential tools in the diagnosis of fish diseases. The present work aimed to identify local intestinal tissue responses and changes in plasma protein profiles of Atlantic salmon fed inactive dry Candida utilis yeast biomass, soybean meal, or combination of soybean meal based diet with various inclusion levels of Candida utilis. A fishmeal based diet was used as control diet. Inclusion of Candida utilis yeast to a fishmeal based diet did not alter the morphology, immune cell population or gene expression of the distal intestine. Lower levels of Candida utilis combined with soybean meal modulated immune cell populations in the distal intestine and reduced the severity of soybean meal-induced enteritis, while higher inclusion levels of Candida utilis were less effective. Changes in the plasma proteomic profile revealed differences between the diets but did not indicate any specific proteins that could be a marker for health or disease. The results suggest that Candida utilis does not alter intestinal morphology or induce major changes in plasma proteome, and thus could be a high-quality alternative protein source with potential functional properties in diets for Atlantic salmon.

Effect of glutathione‐enriched inactive dry yeast on color, phenolic compounds, and antioxidant activity of kiwi wine

Fresh “Xuxiang” kiwifruit juice with or without pomace was used for fermentation with different levels of glutathione‐enriched inactive dry yeast (g‐IDY). After alcohol fermentation, the glutathione (GSH) content, color parameters, phenolic composition, and antioxidant activities of kiwi wine were determined. The results suggested that the addition of g‐IDY to kiwi juice increased GSH, glutathione disulfide, total GSH concentrations, and the total phenolic content of kiwi wine. No obvious effect on individual phenolic content was found in wine with g‐IDY. The color parameters of wines fermented with pomace were remarkably improved with addition of 100 mg/L and 300 mg/L g‐IDY. The supply of g‐IDY improved the antioxidant activities of kiwi wine fermented with pomace. Principal component analysis suggested that with the same supplies of g‐IDY, wine fermented with pomace was better than wine fermented through pure juice in all measurements. PRACTICAL APPLICATIONS: The improvements in color and antioxidant capacity of kiwi wine have always been the focus of kiwi wine brewing. Constant improvement in methods which contribute to high quality of kiwi wine provides crucial support for wine industry and even agriculture development. The aim of this study is to investigate the impacts of glutathione‐enriched inactive dry yeast (g‐IDY) on quality of kiwi wine. These results could provide feasible advices about g‐IDY application to kiwi wine for improving the sensory and nutritional properties.

Potential Applications of High Pressure Homogenization in Winemaking: A Review

High pressure homogenization (HPH) is an emerging technology with several possible applications in the food sector, such as nanoemulsion preparation, microbial and enzymatic inactivation, cell disruption for the extraction of intracellular components, as well as modification of food biopolymer structures to steer their functionalities. All these effects are attributable to the intense mechanical stresses, such as cavitation and shear forces, suffered by the product during the passage through the homogenization valve. The exploitation of the disruptive forces delivered during HPH was also recently proposed for winemaking applications. In this review, after a general description of HPH and its main applications in food processing, the survey is extended to the use of this technology for the production of wine and fermented beverages, particularly focusing on the effects of HPH on the inactivation of wine microorganisms and the induction of yeast autolysis. Further enological applications of HPH technology, such as its use for the production of inactive dry yeast preparations, are also discussed.

Approaches to limit S-off-flavors during white wine fermentation with specific emphasis on yeast nitrogen nutrition

Low molecular weight volatile sulfur compounds are associated with the “reductive” off flavor of wines. The formation of 15 sulfide off-flavor compounds has been monitored with a novel HS-SPME GC-PFPD method and SIDA quantification during white wine fermentation. Since it is known that glutathione (GSH) might buffer nitrogen stress of the yeast but can also lead to an increase of S-off-flavors, the effect of GSH addition was investigated. Fermentations were carried out in triplicate with Riesling musts showing low NOPA concentrations whereby we evaluated the effect of diammonium hydrogen phosphate (DAHP), pantothenic acid, inactive dry yeast based nutrient (IDY) and must oxidation. It could be shown that only the addition of 0.6 g/L IDY increased fermentation speed and completed fermentations. The addition of IDY as well as GSH lead to a significant increase in H2S formation. Total yeast cells were increased by must oxidation thus leading to faster fermentation whereby no significant difference in S-off-flavors could be observed. The addition of GSH did not affect yeast viability but increased significantly the negative effect of IDY addition regardless the S-off-flavor investigated. In all fermentations the use of DAHP reduced significantly S-off-flavor formation and could completely prevent both S-Methyl thioacetate and S-Ethyl thioacetate development. Under nitrogen deficient conditions, GSH can be used in white wine making when combined with DAHP.

Impact of specific inactive dry yeast application on grape skin mechanical properties, phenolic compounds extractability, and wine composition

Foliar treatments using two products based on Saccharomyces cerevisiae inactive dry yeast derivatives with specific formulations for white and red varieties were tested in two consecutive vintages on Vitis vinifera L. cv. Chardonnay, Cortese, and Nebbiolo grown in Piedmont (north-west Italy). The possible elicitor effect of the foliar treatment was assessed at harvest on the chemical composition and mechanical properties of grape berries. The accumulation and extractability of phenolic compounds in Nebbiolo grape skins were also studied. Wines were produced and analysed in terms of technological parameters, color characteristics, free volatile composition, and phenolic compounds. The treatments induced an +16 μm average increase in berry skin thickness, which makes the grapes more resistant to physical damages and pathogenous attacks. In Nebbiolo, this treatment enhanced the accumulation of anthocyanins (+33 mg/kg on average). However, the obtained results pointed out a vintage effect. In 2015, few significant differences between wines made from control and treated grapes were found. Instead, in 2016, Nebbiolo treated wines had a slightly worse chromatic quality as a consequence of lower contents of phenolic compounds, but they were richer in relative amounts of malvidin-3-glucoside.

NUTRIENT DIGESTIBILITY, PRODUCTIVE PERFORMANCE AND SOME SERUM BIOCHEMICAL INDICATORS AS AFFECTED BY SUBSTITUTION OF SOYBEAN MEAL FOR INACTIVE DRY YEAST IN GROWING LAMBS DIET

A total number of 44 growing Ossimi lambs (averaged 4 months of age and 21.59 ± 0.77 kg) were used to assess the effect of soybean meal substitution for inactive dry yeast (IDY) at levels 0, 15, 30 and 45 % on nutrients digestibility, productive performance and some serum biochemical indicators of growing lambs. The results showed that digestibility coefficients of DM, OM, CP, CF, NDF, NFE and nutritive values (DCP and TDN) were increased (P<0.05) for lambs fed IDY at levels 15 and 30 % compared to those fed 0 and 45%. The improvement in nutrients digestibility and nutritive values was higher (P<0.05) for lambs fed IDY at the level 30 % than those fed 15 %. However, the averages of nutrients digestibility and nutritive values for lambs fed IDY at levels 0 and 45 % were comparable. In addition, the averages of final body weight (FBW) were increased (P<0.05) for lambs fed IDY at the level of 30 % than those fed 0, 15 and 45 %. Also, the averages of daily gain (ADG) were increased (P<0.05) for lambs fed IDY at levels of 15 and 30 % in comparison to those fed 0 and 45%. Data showed no significant differences in averages of feed intakes of soybean straw (SSI), DM and TDM among the experimental treatments. However, the intakes of DCP and TDN were greater (P<0.05) for lambs fed IDY at the level of 30 % than those fed 0, 15 and 45 %. The averages of feed conversion (FC) of DM (FC-DM) were improved (P<0.05) for lambs fed IDY at the level 30 % compared with those fed 0 and 45% of IDY. The averages of FC-DCPI and FC-TDNI were better (P<0.05) at 15, 30 and 45 % of IDY than 0 level. The averages of serum total protein (TP) concentrations were higher (P<0.05) for lambs fed IDY at the level of 30 % than those fed the levels of 15 and 45 %. Also, serum albumin levels increased (P<0.05) for lambs fed IDY at 30 % compared with those fed levels 0, 15 and 45 %. Serum globulin concentrations were comparable among the treatments of IDY at 0, 15 and 30 %, being the lowest concentration with feeding IDY at the level of 45 %. There were significant (P<0.05) increase in serum glucose concentrations for lambs fed IDY at the level of 30 % than those fed the levels 15 and 45 %, while they were comparable with those fed IDY at 0 level. The results showed no significant differences in serum concentrations of cholesterol, triglycerides and aspartate transaminase (AST) enzyme activity among the experimental treatments. This study indicated that soybean meal substitution for inactive dry yeast (especially at 30 % level) was effective to improve nutrients digestibility and productive performance accompanying with favourable signs in serum biochemical indicators of Ossimi lambs.

Evaluation of Tempranillo and Albariño SO2-free wines produced by different chemical alternatives and winemaking procedures

In this work physical and chemical alternatives to produce SO2 free wines are shown. The use of inactive yeast strains enriched in glutathione, chitosan, dimethyldicarbonate and different hydrolysable and condensed tannins were assessed in Tempranillo and Albariño wines. The time of addition, mixtures of additives and the use of inert gases were evaluated. In general, no significant differences on the sensory quality were shown when compared sulphited and non-sulphited wines. Both physical and chemical treatments were more effective for Tempranillo wines. In the two studied vintages, non-sulphited Tempranillo wines were better valuated than controls, even when wines were produced only using the application of inert gases. No differences were observed when argon was used instead of carbon dioxide as inert gas. Mixtures of seed and skin tannins with inactive dry yeast enriched in glutathione were the most effective treatments. This work may help winemakers to elaborate healthier wines meeting the current consumer demands.

Study on the effects of dietary supplementation of inactive dry yeast (Saccharomyces cerevisiae) on feed intake, body weight gain and fecal microbiota of crossbreds steers

Study on the effects of dietary supplementation of inactive dry yeast (Saccharomyces cerevisiae) on feed intake, body weight gain and fecal microbiota of crossbreds steers

Contribution of yeast and base wine supplementation to sparkling wine composition.

The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices. In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability. We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine. The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis. The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine. © 2016 Society of Chemical Industry.

NUTRITIVE VALUE OF DIETS WITH INACTIVE DRY YEAST FOR SAANEN GOATS IN THE PERIPARTUM PERIOD

ABSTRACT: The aim was to evaluate the replacement of soybean meal by inactive dry yeast (Saccharomyces cerevisiae) from sugar cane on nutritive value of diets for Saanen goats in the peripartum. Twenty-four Saanen goats (nine primiparous, 55.35 ± 4.55 kg and fifteen multiparous, 73.12 ± 8.51 kg) in prepartum and postpartum periods were distributed in a completely randomized design with a 3 × 2 (diet × parturition order) factorial arrangement. Diets were composed of soybean meal, soybean meal plus dry yeast or dry yeast as protein source, and other ingredients: ground corn, mineral supplement and limestone. The roughage source used was the whole plant corn silage (40% of the total diet). Diets were evaluated as dry matter intake and digestibility of nutrients. The diet with total dry yeast inclusion promoted a reduction on the ether extract intake (postpartum) and ether extract digestibility (prepartum and postpartum), due its lower ether extract content when compared to the other diets. Multiparous goats presented higher intakes for dry matter, nutrients and total digestible nutrients in both evaluated periods due their higher intake capacity. Primiparous goats in postpartum had better coefficients of digestibility for dry matter, organic matter, crude protein and total carbohydrates, and so a higher energetic value, attributed to total digestible nutrients compared to multiparous ones. Dry yeast can replace soybean meal with no changes on nutritive value of diets for Saanen goats.

Inactive dry yeast application on grapes modify Sauvignon Blanc wine aroma

This study investigated the potential to improve wine aroma by applying two inactive dry yeast products (IDYs) at the onset of ripening on Sauvignon Blanc grapes. Both products led to increased reduced glutathione concentrations in the grape juice and corresponding wines, as well as differences in individual higher alcohol acetates (HAAs) and ethyl esters of straight chain fatty acids (EEFAs) at the end of fermentation. After two months of storage, a significantly slower decrease of EEFAs and to a lesser extent of HAAs was found for wines made from grapes with IDY applications. These wines also resulted in significantly slower synthesis of ethyl esters of branched acids, whereas varietal thiols were altered in a product-specific manner. The modifications in the wine chemical composition were also sensorially corroborated. This study showed that vineyard additions of IDY products directly on the grapes at the onset of ripening have a subsequent benefit to the production and preservation of aroma in wines.

Fate of the glutathione released from inactive dry yeast preparations during the alcoholic fermentation of white musts

Background and Aims Inactive dry yeast (IDY) preparations are widely used in the winemaking industry. Among them, glutathione (GSH)-enriched IDY (g-IDY) preparations are claimed to preserve the aroma and colour of white wines due to their antioxidant properties. It remains uncertain, however, if alcoholic fermentation (AF) influences the fate of the GSH released by IDYs and the impact of these preparations on the oxidation status of the wine, which constitutes the aim of this study. Methods and Results The evolution of the GSH concentration of white musts treated with two types of IDYs (a glutathione-enriched IDY, g-IDY, and a fermentative nutrient n-IDY without GSH) and of a control must was determined during AF. In addition, the oxidation status of the musts and the formation of grape reaction product (GRP) derivatives were also monitored. The addition of g-IDYs had little effect on the concentration of reduced GSH in the wines at the end of fermentation. The treatment of musts with IDY preparations, however, reduced the formation of derivatives of GRP and produced more reductive conditions during AF compared with that of control samples. Conclusions These results indicate that GSH released by IDYs is not available at the end of AF, but other yeast components from these preparations could be involved in the greater antioxidant status of musts treated with IDYs. Significance of the Study This study helps to understand the impact of IDY preparations during winemaking, which will assist the optimisation of their formulation for specific wine applications.

Effect of different aging techniques on the polysaccharide and phenolic composition and sensory characteristics of Syrah red wines fermented using different yeast strains

The effect of high levels of the polysaccharide Saccharomyces cerevisiae yeast strain (HPS) and another conventional yeast strain (FERM) on the polysaccharide and phenolic composition of Syrah red wines during alcoholic fermentation and subsequent aging on lees, with or without oak wood chips, and on inactive dry yeast was investigated. The HPS yeast released higher amounts of polysaccharides during alcoholic fermentation than FERM yeast (485gL−1 and 403gL−1, respectively) and after the aging period (516gL−1 and 500gL−1, respectively). The different aging techniques increased the polysaccharide concentration; the concentration was dependent on the aging technique applied. The interaction of the polysaccharides with the phenolic compounds depended on the yeast strain, aging technique, aging period and compound analysed. The HPS wines exhibited better sensory characteristics than the FERM wines after alcoholic fermentation; however, during the aging period, it was difficult to determine which technique produced the best wine due to the interactions of aging technique, aging period and sensory attribute evaluated.