Inactive Biomass Research Articles

Efficient biodecolorization of direct black 22 Azo Dye by Aspergillus tamarii Kita UCP1279 isolated from caatinga soil

The textile industry is a major consumer of water, generating large volumes of wastewater contaminated with harmful substances, particularly azo dyes, which contain aromatic rings, amines, and sulfonic groups that contribute to their environmental toxicity. The use of microbial agents for wastewater treatment offers a cost-effective and sustainable solution, minimizing sludge production. Fungi from the Caatinga biome have demonstrated potential for bioremediation, especially under extreme environmental conditions. This study explores the ability of Aspergillus tamarii Kita to decolorize the azo dye Direct Black 22 (DB22) under varying conditions, including the use of both active and inactive fungal biomass, in static and agitated systems (120 rpm), at a controlled temperature of 30°C. Dye concentrations of 50, 125, and 250 mg/L were evaluated over time intervals of 75, 180, and 180 minutes, respectively. The influence of dissolved oxygen concentration and redox potential on the decolorization process was also investigated to identify optimal treatment conditions. Decolorization efficiency was quantified via spectrophotometric analysis. Results revealed decolorization rates of 100%, 97%, and 63% for the respective dye concentrations, with active biomass in agitated conditions achieving the highest removal rates. The study further demonstrated that dissolved oxygen levels and redox potential are critical factors in enhancing dye decolorization. These findings underscore the bioremediation potential of Aspergillus tamarii Kita in treating textile dye effluents, offering a promising approach to sustainable industrial wastewater management.

Poprawa zdolności sorpcyjnej i zastosowania biosorbentu poprzez immobilizację

The water contamination by toxic metal ions is a worldwide environmental problem. High concentrations are hazardous for all living organisms, causing many disorders and diseases and ecological damages to the surroundings. Biosorption is an innovative technology that employs inactive and dead biomass (bacteria, fungi, algae, biowaste) for the recovery of metals from aqueous solutions. Sulphate-reducing bacteria utilization is one of the options how to prepare applicable sorbent which removes metal ions from water. This material is created as a consequence of bacteria metabolism in anaerobic environment. The use of freely suspended biomass is often impractical and has some disadvantages. In contrast to this, application of immobilized biomass shows more benefits including increased mechanical strength, resistance to chemical environment, easy separation of cells and effluents, high biomass performance and repeated use in many adsorption/desorption cycles. Cell entrapment is the most widely used method for immobilization. In this technique, the cells are enclosed in a polymeric matrix which is porous enough to allow diffusion of substrate to the cells. The aim of this work was to examine and compare the sorption ability of biogenic iron sulphides created by sulphate-reducing bacteria in “free” and “entrapped” form. Precipitates were synthesized in reagent bottles with bacteria culture and growth media Postgate C, at 30 °C during 60 days, subsequently dried and analysed. Prepared samples were immobilized using sodium alginate. Sorption of cadmium from model solutions was realized in 100 ml Erlenmeyer flasks, with sorbent dose 1 g/l, during 24 hours. During experiments, the samples showed a satisfying stability and maximum adsorption capacity achieved 38 mg/g. The results refer to good sorption properties of immobilized samples and their potential for further practical use.

Sorption of Pt(II) on inactive biomass of Aspergillus Niger O-5 treated with cetyltrimethylammonium bromide

Platinum-group cytostatic have been found in natural waters due to the current inefficiency of wastewaters treatment plants. This study was aimed to the assessment of inactive biomass of Aspergillus niger O-5 treated with cetyltrimethylammonium bromide as sorbent for solid phase extraction of [PtCl4]2-complex from aqueous dissolutions for the subsequent development of new water treatment technologies. Maximum sorption capacity of 54 mg·g−1 was achieved at 25 °C under the following experimental conditions: pH of dissolution = 5, biomass mass = 10 mg, dissolution volume = 10 mL, Pt(II) mass = 0.5 mg and contact time = 30 min. The good fitting of the model of Ho and the increase of sorption capacity up to 102 mg·g−1 with temperature suggested the chemisorption as predominant mechanism. The mean free energy of adsorption was higher than 8 kJ·mol−1 for all temperatures, which also indicated the chemical character of the interaction. The absolute value of the standard molar Gibbs free energy of formation increased from 3.032 to 8.336 kJ· mol−1 with temperature signifying that the sorption was thermodynamically favored, occurred spontaneously and the degree of spontaneity increased with temperature. The positive value (32.3 kJ·mol−1) of the standard molar enthalpy qualified the sorption as an endothermic process, after which the adsorbent/sorbate system became more thermodynamically stable.

Evaluation of vat blue removal by Aspergillus niger using scanning electron microscopy and glucose oxidase enzyme activity

AbstractThe use of fungi to reduce dye removal in water from diverse industries can be an efficient option for solving environmental problems. However, the mycoremediation processes involved in color removal are unknown. In this article, Aspergillus niger CDBB‐H‐175 was applied for vat blue discoloration (DC). Hence, inactive biomass by two methods (metabolic inhibitor and heat) and active biomass were studied by scanning electron microscopy and enzyme activities to determine the vat blue DC. When using active biomass, 62% of DC, glucose oxidase (GOX) enzyme activity, 1.19 U/mL and 1.43 mg/L of H2O2 were obtained. It was observed that 68% of DC was due to the degradation process and 32% was due to biomass sorption. When the biomass was inactivated with sodium azide, DC increased by 90% (1.45‐fold), low GOX activity (0.138 U/mL), and H2O2 (0.25 mg/L) were detected. High DC might be due to a high dye sorption in hyphae, since an increase in the thickness and morphology of the hyphae were observed by SEM. Inactivated biomass through heating, showed a 69.5% of DC, where 0.01 U/mL GOX and 0.25 mg/L H2O2 were reached, demonstrating that inactivation of A. niger biomass by sodium azide can be considered as an alternative treatment for colored wastewater.

ПРОБИОТИЧЕСКАЯ КОРМОВАЯ ДОБАВКА «ЦИТРОСПОРИН» И ЕЕ ВЛИЯНИЕ НА БИОХИМИЧЕСКИЙ И ИММУНОЛОГИЧЕСКИЙ СТАТУС СВИНЕЙ НА ОТКОРМЕ

The article presents data on the study of the effectiveness of a probiotic supplement based on inactivated biomass of the fungus R.citrinum and B.subtilis on biochemical blood parameters and immunological status in pigs on feed. The study was conducted on 15 crossbreeds (F1:(DhL) during the fattening period with an initial live weight of 20–25 kg at the age of 45 days. The duration of the study is 45 days. The inclusion of the probiotic additive Cytosporin based on B.subtilis VKM B-3171D, In the composition of full-fledged compound feeds of experimental groups of growing fattened young pigs. B.licheniformis VKM B-3172D, inactivated biomass of the fungus R.citrinum VKM B-4930D provides an increase in urea and creatinine by 20.9% (P<0.05) and 6%, respectively, and glucose concentration by 13.5% and 25.4% (P<0.05) higher relative to the control group. During the study, a decrease in the number of salmonella and enterococcus in the experimental groups was reliably recorded, which confirms the effectiveness of the use of the probiotic additive Citrosporin.

ПРОБИОТИЧЕСКАЯ КОРМОВАЯ ДОБАВКА «ЦИТОСПОРИН» И ЕЕ ВЛИЯНИЕ НА ПРОДУКТИВНОСТЬ СВИНЕЙ НА ОТКОРМЕ

The article presents data on the study of the effectiveness of a "Cytosporin" based on B. Subtilis VKM B-3171 D, B. Licheniformis VKM B-3172D, inactivated biomass of the fungus R. citrinum VKM B-4930D on the indicators of digestibility, digestibility and zootechnical indicators in pigs on rearing. The study was carried out on 15 heads of local hogs (F1:(DxL) during the fattening period with an initial live weight of 20‒25 kg at the age of 45 days. The duration of the study is 45 days. The inclusion of a probiotic additive inactivated biomass of the mushroom in the composition of full-fledged compound feeds of experimental groups of growing fattened young pigs. "Cytosporin" provide an increase in average daily live weight gain by 0.79‒3.6% compared to control animals. In growing young pigs of experimental groups that received the studied probiotic additives, the coefficient of digestibility of dry matter increased by 0.24–2.4%, organic matter – by 0.6–1.27%, protein – by 0.17‒2.34% compared to control animals.

Optimization of Chromium (VI) Adsorption using Microalgae Biomass (Spirulina sp.) and its Application in Leather Tannery Waste

<p class="E-JOURNALAbstractBodyEnglish">This study was conducted to examine the adsorption of Cr (VI) metal using Spirulina sp (inactive) biomass and its application in leather tannery wastewater. The treatment was carried out to determine the influence of independent variables on Cr (VI) adsorption with variations in pH, contact time, and metal solution concentration. The values of the solution pH, adsorption time, and concentration of the best metal solution were used to determine the center points of the optimization variables through Response Surface Methodology (RSM). The results showed that based on the FTIR test, macromolecules present in Spirulina sp biomass included amino, carboxylate, and hydroxy groups. The combination of factor variables that produced the optimum response was at pH 3.165, contact time of 66.58 minutes, and Cr (VI) metal ion solution concentration of 22.9 mg/L, resulting in a Cr (VI) adsorption efficiency of 69.66%. The adsorption pattern was included in the Freundlich adsorption isotherm, and the application of Spirulina sp biomass adsorbent to tannery waste reduced the concentration of Cr (VI) from 3.9 g/L to an undetectable level at <1.4 g/L.</p>

Pb2+ biosorption by Serratia marcescens CCMA 1010 and its relation with zntR gene expression and ZntA efflux pump regulation

Global concerns about the preservation and restoration of aquatic environments are rising and pollution related to heavy metals is one of the main worries. Indeed, this issue has been a challenge for the metallurgical industry and other activities associated with metal contamination. Thus, over time, several physical and chemical methods have been developed and applied to remove metals from water. However, these methods can be associated with high costs, and bioremediation using plants, fungi, and bacteria is considered a viable alternative. This paper reports experiments on lead removal from an aqueous medium using active and inactive Serratia marcescens CCMA 1010 and a study on the effect of lead in this bacterial strain regarding the expression of the zntR gene, which produces the zntR protein known to have a role as a regulator of the ZntA efflux pump of metals (Pb2+, Cd2+, Zn2+). The amount of removed Pb2+ by active biomass remained below ∼25 mg/L for the initial concentrations tested up to 120 mg/L but increased to removals of ∼70 and ∼167 mg/L for the tested concentrations of 220 and 300 mg/L, respectively. On the other hand, the removal of Pb2+ by inactive biomass increased in direct relation to the initial tested concentration, with removed percentages around 25 %. Interestingly, in cultures with 15 and 60 mg/L Pb2+ the expression of zntR was 27 to 74 and 87 to 177 times lower (respectively) than in cultures without Pb2+, while in cultures with 120 mg/L Pb2+, the zntR expression was just 1.3 to 9.3 times lower than in the absence of Pb2+. The results confirm the potential of S. marcescens CCMA 1010 for Pb2+ biosorption, the presence of Pb2+ resistance mechanisms in this strain, and contribute to a better understanding of the ZntA transmembrane protein regulation.

Comparison between the Nature and Activity of Silver Nanoparticles Produced by Active and Inactive Fungal Biomass Forms on Cervical Cancer Cells

Silver nanoparticles (SNPs) can be produced by active and inactive forms of biomass, but their properties have not been compared. Recent research is attempting to reveal their differences in shape, size, amount, antibacterial activity, cytotoxicity, and apoptosis induction. The biomass of Fusarium oxysporum was divided into four groups and pretreated in the following devices: room temperature (RT) and refrigerator (for preparation of active biomass forms), autoclave, and hot air oven (for preparation of inactive biomass forms). Samples were floated in ddH2O, and SNPs were produced after the addition of 0.1699 g/L AgNO3 in the ddH2O solution. SNP production was confirmed by visible spectrophotometry, transmission electron microscopy (TEM) and X-ray diffraction (XRD). SNPs were washed, and their concentration was determined by measuring atomic emission spectroscopy with inductively coupled plasma (ICP-OES). For antibacterial activity, the plate-well diffusion method was used. MTT and Annexin V-FITC/propidium iodide assays were used for cytotoxicity and apoptosis induction, respectively. The maximum absorbance peaks for SNPs pretreated in RT, refrigerator, autoclave, and hot air oven were 404, 402, 412, and 412 nm, respectively. The SNPs produced were almost the same shape and size, and the XRD results confirmed the presence of SNPs in all samples. Due to the differences in the type of bacterial strains used, the SNPs produced showed some differences in their antibacterial activity. The MTT assay showed that the amounts of SNPs in their IC50 dose based on the results of ICP-OES were 0.40, 0.45, 0.66, and 0.44 ppm for the samples pretreated in the hot air oven, autoclave, and refrigerator, and RT, respectively. The apoptosis induction results showed that the biologically engineered SNPs induced more apoptosis (about 34.25%) and less necrosis (about 13.25%). In conclusion, the type and activity of SNPs produced by the active and inactive forms of fungal biomass did not change. Therefore, use of the inactive form of biomass in the future to avoid environmental contamination is reccommended.

Evaluation of Residual Yeast from Brewery Industry for Inactive Biosorption of Selenium from Industrial Wastewater: a Case Study.

In this study, the second-life application of Saccharomyces cerevisiae obtained from brewery wastewater was evaluated in the biosorption of Se(IV) (Na2SeO3) sorbate in residue generated from a fine chemical industry. Biosorption experiments were carried out with different Se(IV) concentrations (A = 7.5 to 30.0 mg L-1 dissolved in deionized water or industrial effluent) and different biosorbent concentrations (B = 2.0 to 52.5 g L-1, dry mass). Inactive microbial biomass was evaluated in a wet and dehydrated state. The highest selenium removal efficiency (biosorption efficiency-R = 97.5%) was achieved with the same concentrations of sorbate in deionized water, using 24.0 g L-1 of wet cells. In contrast, the industrial effluent treatment showed lower biosorption efficiency (R = 83.3%) due to a large amount of other salts in the medium, mainly sulphur. Overall, the use of smaller amounts of biosorbent had a biosorption capacity of approximately five times greater than when 24.0 g L-1 in industrial effluent treatment was used. However, as reducing the concentration of the contaminant contained in the wastewater is the primary goal of this study, a more significant amount of biosorbent is recommended.

240 Lactobacillus crispatus probiotic for skin antiaging benefits

We previously showed that significantly lower populations of Lactobacilli were observed in a cohort of aged donors with no Lactobacillus crispatus (LBC) detectable in the wrinkle hollows. The aim of our study was to demonstrate LBC efficacy in vitro, then to design a formula to maintain an efficient delivery of LBC living probiotic to show significant antiaging clinical results. The LBC was isolated from healthy skin and checked by full genome sequencing. After optimized growth a part of the biomass was kept alive and freeze-dried (109 colony forming units/g (cfu/g), whereas the other part was heat killed. The effect on the synthesis of collagen type I and V was evaluated on fibroblast by DELFIA method. We first tested the viability of the LBC with several formulating ingredients up to 24h. Some formulations were then evaluated up to 168 days at different temperatures. One formulation was used to deliver the probiotic strain on 29 Caucasian women (45-65 years old). The dermis density was measured after 3 and 8 weeks by ultrasound imaging coupled to image analysis, and wrinkle were analyzed by VISIA CR imaging. The probiotic stimulated collagen I and V content versus untreated (+133% and +55%) whereas inactivated bacteria were inefficient. In formulation, the probiotic strain was viable and stable in some natural oil-based emollients for several weeks, but for only up few hours in some emulsifiers and mild preservatives. Thus, we designed an application routine for the clinical test in which an oily serum of concentrated probiotic booster was diluted before application in a neutral emulsion to deliver 5 105 cfu/g on the skin. After 2 months of twice-daily application, the density of the sub-epidermal zone was significantly increased vs baseline by 11% and of the dermis by 6% (+5% vs placebo). The same tendency was obtained for forehead wrinkle thickness improvement. We demonstrated the benefits of skin native LBC living probiotic bacteria compared to inactivated biomass to stimulate collagen in vitro. Moreover, the LBC was stable in some formulations and improved dermis density and wrinkle appearance in vivo.

Adaptation of a microbial community to demand-oriented biological methanation

BackgroundBiological conversion of the surplus of renewable electricity and carbon dioxide (CO2) from biogas plants to biomethane (CH4) could support energy storage and strengthen the power grid. Biological methanation (BM) is linked closely to the activity of biogas-producing Bacteria and methanogenic Archaea. During reactor operations, the microbiome is often subject to various changes, e.g., substrate limitation or pH-shifts, whereby the microorganisms are challenged to adapt to the new conditions. In this study, various process parameters including pH value, CH4 production rate, conversion yields and final gas composition were monitored for a hydrogenotrophic-adapted microbial community cultivated in a laboratory-scale BM reactor. To investigate the robustness of the BM process regarding power oscillations, the biogas microbiome was exposed to five hydrogen (H2)-feeding regimes lasting several days.ResultsApplying various “on–off” H2-feeding regimes, the CH4 production rate recovered quickly, demonstrating a significant resilience of the microbial community. Analyses of the taxonomic composition of the microbiome revealed a high abundance of the bacterial phyla Firmicutes, Bacteroidota and Thermotogota followed by hydrogenotrophic Archaea of the phylum Methanobacteriota. Homo-acetogenic and heterotrophic fermenting Bacteria formed a complex food web with methanogens. The abundance of the methanogenic Archaea roughly doubled during discontinuous H2-feeding, which was related mainly to an increase in acetoclastic Methanothrix species. Results also suggested that Bacteria feeding on methanogens could reduce overall CH4 production. On the other hand, using inactive biomass as a substrate could support the growth of methanogenic Archaea. During the BM process, the additional production of H2 by fermenting Bacteria seemed to support the maintenance of hydrogenotrophic methanogens at non-H2-feeding phases. Besides the elusive role of Methanothrix during the H2-feeding phases, acetate consumption and pH maintenance at the non-feeding phase can be assigned to this species.ConclusionsTaken together, the high adaptive potential of microbial communities contributes to the robustness of BM processes during discontinuous H2-feeding and supports the commercial use of BM processes for energy storage. Discontinuous feeding strategies could be used to enrich methanogenic Archaea during the establishment of a microbial community for BM. Both findings could contribute to design and improve BM processes from lab to pilot scale.

524 Better performance of live probiotic over inactivated biomass on skin density

In a previous prevalence analysis study, we showed that lactic acid bacteria occurrence significantly decreases into the wrinkle zone of old volunteer in vivo. Furthermore, relative abundance analysis particularly revealed the absence of a beneficial bacteria, Lactobacillus crispatus, in the same area. This discovery drove us to develop solutions to favor more desirable and young skin phenotype through a direct contact with this valuable bacterium or an extract. In this aim, we tested two conditions including two forms of Lactobacillus crispatus: the living biomass and the inactivated biomass.

A mathematical model of quorum quenching in biofilm colonies and its potential role as an adjuvant for antibiotic treatment

We extend a previously presented mesoscopic (i.e. colony scale) mathematical model of the reaction of bacterial biofilms to antibiotics. In that earlier model, exposure to antibiotics evokes two responses: inactivation as the antibiotics kill the bacteria, and inducing a quorum sensing based stress response mechanism upon exposure to small sublethal dosages. To this model we add now quorum quenching as an adjuvant to antibiotic therapy. Quorum quenchers are modeled like enzymes that degrade the quorum sensing signal concentration. The resulting model is a quasilinear system of seven reaction-diffusion equations for the dependent variables volume fractions of upregulated (protected), downregulated (unprotected) and inert (inactive) biomass [particulate substances], and for concentrations of a growth promoting nutrient, antibiotics, quorum sensing signal, and quorum quenchers [dissolved substances]. The biomass fractions are subject to two nonlinear diffusion effects: (i) degeneracy, as in the porous medium equation, where biomass vanishes, and (ii) a super-diffusion singularity where as it attains its theoretically possible maximum. We study this model in numerical simulations. Our simulations suggest that for maximum efficacy quorum quenchers should be applied early on before quorum sensing induction in the biofilm can take place, and that an antibiotic strategy that by itself might not be successful can be notably improved upon if paired with quorum quenchers as an adjuvant.

Kinetic Modeling of Cd(II) and Pb(II) Biosorption from Aqueous Solution by Inactive Biomass of Nannochloropsis oculata Microalgae

Kinetic Modeling of Cd(II) and Pb(II) Biosorption from Aqueous Solution by Inactive Biomass of Nannochloropsis oculata Microalgae

Efficient removal potential of Microbacterium sp. strain 1S1 against arsenite isolated from polluted environment

A multiple metal resistant Microbacterium sp. strain 1S1, tolerated arsenite and arsenate upto 75 and 520 mM, was isolated from industrial wastewater. The arsenite surface adsorption and uptake into the bacterial cells, exposed to 15 mM arsenite, were confirmed through Scanning Electron Microscope (SEM), Energy Dispersive X-ray (EDX), and Fourier-transform infrared spectroscopy (FTIR) analyses. The cell physiology of strain 1S1 altered in arsenite exposure and the percent increase in GSH/GSSG ratio and NPSHs concentration was up to 40.0 and 78.50%. Furthermore, 240% increase in catalase provided evidence that arsenite induces hydrogen peroxide mediating oxidative stress. The bacterial cells growing in a rich medium with 15 mM arsenite were able to oxidize 98% arsenite after 96 h of incubation, and the inactivated biomass of the bacterium at 1 g per liter removed 99% of 15 mM arsenite after 10 h incubation. The harboring of multiple resistance strategies and appreciable arsenic oxidizing potential make strain 1S1 an impending foundation for green chemistry to exterminate environmental arsenite.

Characterization of spatiotemporal electroactive anodic biofilm activity distribution using 1D simulations

Activity distribution limitation in electroactive biofilm remains an unclear phenomenon. Some observations using confocal microscopy have shown notable difference between activity close to the anode and activity at the liquid interface. A numerical model is developed in this work to describe biofilm growth and local biomass segregation in electroactive biofilm. Under our model hypothesis, metabolic activity distribution in the biofilm results from the competition between two limiting factors: acetate diffusion and electronic conduction in the biofilm. Influence of inactive biomass fraction (i.e. non-growing biomass fraction) properties (such as conductivity and density) is simulated to show variation in local biomass distribution. Introducing a dependence of effective diffusion to local density leads to a drastic biomass fraction segregation. Increasing density of inactive fraction reduces significantly acetate diffusion in biofilm, enhances biomass activity on the outer layer (liquid/biofilm interface) and maintains inner core largely inactive. High inactive fraction conductivity enhances biomass activity in the outer layer and enhances current production. Hence, investment in extracellular polymer substance (EPS), anchoring redox components, is benefit for biofilm electroactivity. However, under our model hypothesis it means that conductivity should be two order lower than biofilm conductivity reported in order to observe inner core active biomass segregation.

Kinetic study and thermodynamic equilibrium modeling of the Co(II) and Mn(II) bioadsorption using the Rhodococcus opacus strain

Microbial biomass is considered a renewable and environmentally friendly resource. Thus, the research conducted a kinetic study and thermodynamic equilibrium modeling of the cobalt (Co) and manganese (Mn) bioadsorption process using the Rhodococcus opacus (RO) strain as a biosorbent. The inactive biomass subjected to 0.1 M NaOH pretreatment was brought into contact with synthetic solutions of Co and Mn. The experimental data for the Co(II) and Mn(II) bioadsorption process were fit to the Langmuir model with kads of 0.65 and 0.11 L.mg-1, respectively. A better statistical fit was also obtained for the pseudo-second order kinetic model (R2Co(II) = 0.994 and R2Mn(II) = 0.995), with 72.3% Co(II) and 80% Mn(II) removals during the first 10 min. In addition, a higher affinity of RO for the Co(II) ion was observed, with maximum uptake values of 13.42 mg.g-1; however, a higher adsorption rate was observed for Mn(II) ion (k = 0.21 g.mg-1.min-1 at 318 K). The bioadsorption process was spontaneous and dependent on temperature, being endothermic and irreversible for the Co(II) ion (∆H = 2951.91 J.mol-1) and exothermic and reversible for the Mn(II) ion (∆H = -2974.8 J.mol-1). The kinetic and thermodynamic equilibrium modeling allowed to identify the main mechanisms involved in the biosorption process of both metals.

Safety and efficacy of a feed additive consisting of astaxanthin-rich Phaffia rhodozyma for salmon and trout (Igene Biotechnology, Inc.).

Following a request from the European Commission, the FEEDAP Panel was asked to deliver a scientific opinion on the safety and efficacy of astaxanthin (ATX)‐rich Phaffia rhodozyma. The additive, belonging to the category ‘sensory additives’ and the functional group ‘substances which, when fed to animals, add colours to food of animal origin’ is intended to be used in feed for salmon and trout from an age of six months onwards up to a maximum content of 100 mg ATX/kg complete feed. The product is produced by the telemorph of Phaffia rhodozyma, Xanthophyllomyces dendrorhous, and it is declared to contain 995 g dried inactivated biomass and 5 g ascorbic acid per kg additive. The main active principle of the additive is ATX; however, the FEEDAP Panel noted that some other carotenoids are also present in lower quantities. The minimum ATX concentration is specified to be 5,000 mg per kg additive. The yeast Xanthophyllomyces dendrorhous is considered by EFSA to be suitable for the qualified presumption of safety (QPS) approach to safety assessment; therefore, the use of the production strain in the production of the additive would not raise any safety concern for the target species, the consumers of products from animals fed the additive and the environment. In the absence of a tolerance study with the additive, the FEEDAP Panel cannot conclude on the safety for the target species. In the absence of residue and toxicity data of ATX, no final conclusions on the safety for the consumer can be drawn. The FEEDAP Panel concluded that the additive is irritant to skin and eyes, and a skin and respiratory sensitiser, although exposure by inhalation is likely low. The FEEDAP Panel considers that ATX from the biomass does not pose a significant additional risk to the environment compared with other natural sources of ATX. In absence of adequate evidence, no conclusion can be made on the efficacy of the additive.

Biosorption of lead (Pb(II)) ions by active and inactive biomass of heavy metal tolerant fungal biomass isolated from the polluted sites

Biosorption of lead (Pb(II)) ions by active and inactive biomass of heavy metal tolerant fungal biomass isolated from the polluted sites