Sustainable agricultural practices are needed to find a solution to the problem of soil erosion and decreased soil quality. A study was conducted during the 2021/2022 and 2022/2023 cropping seasons to evaluate the synergistic effect of the tillage system (TS) and manure rates (MR) on selected soil properties at the University of Limpopo Experimental Farm (Syferkuil) and University of Venda Experimental Farm (UNIVEN). The experiment had a split plot design with three replications. The main plots used conventional (CON) and in-field rainwater harvesting (IRWH) tillage systems, while subplots used poultry and cattle manure at rates of 0, 20, and 35 t ha−1. Bulk density (BD), aggregate stability (AS), pH, total N, organic carbon (OC), available P, and exchangeable cations (Ca, Mg, and K) were determined. IRWH significantly increased AS in the 0–20 cm soil layer at Syferkuil. TS × MR interaction significantly influenced AS and total N in the 20–40 cm soil layer during the 2022/2023 season at Syferkuil. IRWH significantly increased Mg content in the 2021/2022 season and total N, OC, and Mg content in the 2022/2023 season at Syferkuil over CON. At UNIVEN, CON significantly increased total N, whereas IRWH increased available P in the 2022/2023 season. MR significantly increased AS, exchangeable Ca, Mg, and K at both sites. At Syferkuil, MR significantly increased total N, OC, and available P during both seasons, whereas at UNIVEN the significant increase was observed on OC and available P during both seasons and total N in the 2021/2022 season. It was found that IRWH and poultry manure (35 t ha−1) improved most soil properties at both sites; however, this study recommends long-term experiments to investigate the combined effect of IRWH and manure rate on soil properties to validate the findings observed in this study.
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