Improve Crop Productivity Research Articles

Hormopriming and duration of priming interaction on germination, emergence and subsequent growth productivity performance in pechay

This study investigated the potential of Ficus nota water (FNW) as a natural soaking agent to enhance seed germination, emergence, and subsequent growth performance in Pechay (Brassica rapa subsp. chinensis). Utilizing varying FNW concentrations (0%, 50%, and 100%) and seed soaking durations (2, 4, 6 hours), the research aimed to identify optimal treatment combinations for maximizing pre-transplant vigor and post-transplant productivity of Pechy. The results revealed a significant interaction between FNW concentration and soaking time, accelerating germination onset and promoting germination percentage. Notably, the 50% FNW treatment coupled with the 4-hour soaking duration expedited germination by 60% compared to the untreated control. This optimal priming combination also positively influenced seedling height, root proliferation, and biomass accumulation during the pre-transplant nursery stage. Post-transplantation, FNW-primed seedlings exhibited taller stems and broader leaves compared to non-primed controls. However, despite enhancing vegetative growth, priming did not translate into increased biomass yields, suggesting potential trade-offs in resource allocation patterns. The findings highlight the efficacy of FNW in expediting germination and enhancing nursery vigor, while underscoring the need for further investigations into the mechanisms governing the translation of early growth promotion into marketable yields under field conditions. This research contributes valuable insights into the application of natural plant extracts for optimizing seed priming protocols and potentially improving crop productivity.

Manipulating the light spectrum to increase the biomass production, physiological plasticity and nutritional quality of Eruca sativa L

The extensive development in light-emitting diodes (LEDs) in recent years provides an opportunity to positively influence plant growth and biomass accumulation and to optimize biochemical composition and nutritional quality. This study aimed to assess how different light spectra affect the growth, photosynthesis and biochemical properties of Eruca sativa. Therefore two LED lighting modes - red:blue (RB, 1:1) and red:green:blue (RGB, 2:1:2) were compared to the conventional white light fluorescent tubes (WL). Plant biomass, photosynthetic performance, several antioxidants, polyamines and nitrates contents were analyzed across different treatments. The plant growth was affected by the light quality - the presence of green light in the spectrum resulted in smaller plants and leaves, and correspondingly less biomass. RB spectral mode enhanced the total antioxidant and guaiacol peroxidase activity, pigments, flavonoids, polyphenols, ascorbate and polyamines contents. This effect under RB was combined with better leaf development compared to RGB and less nitrate in the leaves among all treatments. The RB light generated modifications in polyamines, which are interrelated with the nitrate content, further induce important metabolite and antioxidant changes. Both RB and RGB enhanced photosynthesis. The afterglow thermoluminescence band varied according to leaves development, being higher in RB and WL as a consequence of their faster growth. The RB light spectrum was found to be the most efficient for promoting the growth, biochemical composition, and overall quality of Eruca sativa compared to RGB and WL. These findings suggest that RB LEDs can be an effective tool for improving crop production in controlled environments.

Characterization of Plant Growth Promoting Rhizobacteria and Their Benefits on Soybean Growth

Background: Soybean is the most important legume in the world and its seeds contains 40% protein and 20% oil. In recent years, more attention has been paid to the use of plant growth-promoting (PGP) rhizobacteria as a biofertilizer alternative to chemical fertilizers, which may pose risks to the environment. The rhizobia inoculation of soybean is a sustainable practice to induce atmospheric nitrogen fixation and subsequently improve crop productivity and soil fertility. Methods: In this study, 19 indigenous rhizobia isolated from soybean were tested in vitro for their plant growth-promoting properties (PGPR), while genotypic characterization included sequencing of the 16S rRNA gene. Result: Morphological characterisation has shown that all of the isolates were rod-shaped gram negative bacteria. The sequencing has shown that 12 out of 19 isolates belong to the genus Pseudomonas, four of which belong to P. fluorescens species. Isolates which belong to the Pseudomonas genus have shown the highest ability of indole-3-acetic acid synthesis, phosphorous solubilization and along with isolates SGN6, SGN7 and SGS4 (Sphingomonas sanguinis) potassium solubilisation too. All the isolates from Pseudomonas genus as well as those belonging to B. japonicum species have shown the protease synthesis abilities while amylase synthesis abilities was observed only in the isolate SAK2 (P. chlororaphis). The most efficient strains in in vitro biological nitrogen fixation assay belong to Pseudomonas and Rhizobium genera. Most of the isolates were positive to the organic acid production while all of them have shown the ability of the exopolysaccharide production.

The Application of a Foliar Spray Containing Methylobacterium symbioticum Had a Limited Effect on Crop Yield and Nitrogen Recovery in Field and Pot-Grown Maize.

In this study, the effectiveness of an inoculant containing a nitrogen (N)-fixing microorganism (Methylobacterium symbioticum) was evaluated on maize (Zea mays L.) grown both in the field (silage maize) and in pots over two years (2021 and 2022). The field trial included the following two treatments: with (Yes) and without (No) the inoculant. The pot experiment was designed as a factorial arrangement with two factors: the application of the inoculant (Yes and No) and N applied to the soil (0, 0.4, 0.8, and 1.6 g pot-1). In the field, total dry matter yield (DMY) did not differ significantly between treatments, although the average DMY was higher in the inoculant treatment. In pots, the total DMY varied significantly across all N rates but was only significantly affected by the inoculant application in 2022. N fixation estimates in the field were 58.8 and 14.5 kg ha-1 for 2021 and 2022, respectively, representing 23.7% and 9.1% of the N recovered in the aboveground plant parts. In pots, the estimated fixed N values were -49.2 and 199.2 mg pot-1 in 2021 and 2022, respectively, which corresponded to -5.2% and 18.5% of the N found in the aboveground plant parts. Considering the average values obtained across the four cultivation conditions, there was a positive outcome for the treated plants. However, these values cannot be considered significant when compared to nitrogen removal in maize crops. A commercial product should provide an unequivocal and quantitatively relevant contribution to plant nutrition, which did not appear to be the case. Thus, for this inoculant to provide reliable guarantees of positive outcomes for farmers and become a useful tool in promoting more sustainable agriculture, further studies appear necessary. These studies should aim to determine in which crops and under what cultivation conditions the application of the inoculant is truly effective in enhancing N fixation and improving crop productivity.

Integrative multi-omic analysis identifies genes associated with cuticular wax biogenesis in adult maize leaves.

Studying the genetic basis of leaf wax composition and its correlation with leaf cuticular conductance (gc) is crucial for improving crop productivity. The leaf cuticle, which comprises a cutin matrix and various waxes, functions as an extracellular hydrophobic layer, protecting against water loss upon stomatal closure. To address the limited understanding of genes associated with the natural variation of adult leaf cuticular waxes and their connection to gc, we conducted statistical genetic analyses using leaf transcriptomic, metabolomic, and physiological data sets collected from a maize (Zea mays L.) panel of ∼300 inbred lines. Through a random forest analysis with 60 cuticular wax traits, it was shown that high molecular weight wax esters play an important role in predicting gc. Integrating results from genome-wide and transcriptome-wide studies (GWAS and TWAS) via a Fisher's combined test revealed 231 candidate genes detected by all three association tests. Among these, 11 genes exhibit known or predicted roles in cuticle-related processes. Throughout the genome, multiple hotspots consisting of GWAS signals for several traits from one or more wax classes were discovered, identifying four additional plausible candidate genes and providing insights into the genetic basis of correlated wax traits. Establishing a partially shared genetic architecture, we identified 35 genes for both gc and at least one wax trait, with four considered plausible candidates. Our study enhances the understanding of how adult leaf cuticle wax composition relates to gc and implicates both known and novel candidate genes as potential targets for optimizing productivity in maize.

Sustainable farming practices enhance bacterial diversity and nutrient levels in sorghum rhizosphere soil

This research employs metagenomic analysis to explore bacterial diversity in sorghum rhizosphere soil in response to various sustainable farming practices and their impact on soil fertility. The field experiment with cotton-sorghum cropping system was conducted since 2020 and from 3rd cycle sorghum experiment, the soils were collected and subjected to metagenomics and nutrients availability analysis. We evaluated the impact of different farming approaches: Natural Farming (NF), Organic Farming (OF), integrated crop management (ICM), along with a control (No input farming). The results underscores the prevalence of Proteobacteria phylum across all farming practices, while Actinobacteria, Bacteroidetes and Firmicutes showed increased presence due to altered soil management and nutrient availability changes. Genus-level analysis revealed shifts in dominant genera, including Acinetobacter, Sphingomonas, Bacillus, and Candidatus nitrososphaera, across different farming practices. Post-harvest soil analysis indicated varied nutrient levels, with ICM showing enhanced nitrogen, phosphorus, and potassium availability. ICM, utilizing a balanced mix of organic and inorganic fertilizers, promoted sorghum growth, yielding higher plant height and total dry matter compared to OF and NF, which performed similarly. The study emphasizes the efficacy of a balanced approach like ICM for improving crop production and soil nutrient availability. However, all diversified farming practices exhibited high soil biological diversity compared to the control, highlighting the necessity for continuous long-term soil monitoring across different farming practices to stabilize soil microbiomes, ensure nutrient availability, and support sustainable crop production.

Seminar Proceedings

This collection of articles includes recent research and innovations presented at a national seminar focused on the application of biotechnologies in agriculture. The topics covered include sustainable agricultural practices, genetic advancements, and biotechnological interventions aimed at improving crop productivity and environmental sustainability.

Characterization of differences in seed endophytic microbiome in conventional and organic rice by amplicon-based sequencing and culturing methods.

In this paper, we studied the differences in the endophytic microbial composition of rice seeds grown in conventional and organic farming systems. Our results demonstrate a greater bacterial diversity in conventional farming, while organic farming showcases a higher fungal diversity. Additionally, our research reveals the ability of seed bacterial endophytes to inhibit the growth of three fungal pathogens responsible for causing seedling blight in rice. This study provides valuable insights into the potential use of beneficial seed microbial endophytes for developing a novel microbiome-based strategy in the management of rice diseases. Such an approach has the potential to enhance overall plant health and improve crop productivity.

Effect of integrated nutrient management on the growth and yield of YellowSarson (Brassica rapa var. yellow sarson) under guava (Psidium guajava) basedagri-horti system

An experiment was conducted during the winter (rabi) season of 2021–22 to evaluate the effect of integrated nutrient management on the growth and yield of yellow sarson under guava based agri-horti system. The field ex- periment comprised 8 treatment, viz., T1 , Control; T2 , 100% RDF + 30 kg S + 5 kg Zn; T3 , 50% RDF + 15 kg S + 2.5 kg Zn + 2.5 t/ha FYM+ 2 spray of nano urea; T4 , 50% RDF + 15 kg S + 2.5 kg Zn + 5 t/ha FYM+ one spray of nano urea; T5 , 75% RDF + 22.5 kg S + 3.7 kg Zn + 2.5 t/ha FYM + 2 spray of nano urea; T6 , 75% RDF + 22.5 kg S + 3.7 kg Zn +5 t/ha FYM + 1 spray of nano urea; T7 , 100 % RDF + 30 kg S + 5 kg Zn + 2.5 t/ha FYM; T8 , 100% RDF + 30 kg S + 5 kg Zn + 5 t/ha FYM, was laid out in randomized block design, with 3 replications. The results revealed that application of 75% RDF + 22.5 kg S + 3.7 kg Zn +2.5 t/ha FYM + 2 spray of nano urea at 30-35 DAS and pre-flow- ering stage (T5 ) gave significantly higher growth and yield attributes such as plant height (141.03 cm), dry matter accumulation (37.76 g/plant), no. of siliqua (142/plant), siliqua length (6.67 cm), test weight (3.43 g) as well as seed yield (1.49 t/ha) and stover yield (3.54 t/ha) as compared to control. The highest amount of nitrogen, phos- phorus, potassium and sulphur uptake by plant was also observed in the treatment T5. These findings provide valuable insights for farmers and researchers aiming to optimize nutrient management strategies and improve crop productivity in similar agri-horti systems.

Biopriming using microbial consortia and biostimulants as a technique to increase seed quality of Solanum melongena L. by modifications in morphological and metabolic constituents

Brinjal (Solanum melongena L.) is a widely cultivated vegetable in tropical and subtropical regions. High-quality seeds are crucial for successful crop production, necessitating rapid and uniform field emergence. In the new wave of the Green Revolution, organic wastes were replaced by agrochemicals. This study was conducted from January to March 2024 to standardize biopriming techniques for brinjal using various organic inputs and microbial cultures to improve seed quality. Brinjal seeds cv. PLR 2 were treated with organic inputs (Panchagavya, Egg fermented extract, Fish fermented extract and effective microorganism solution) and liquid microbial cultures (Azospirillum and Pink Pigmented Facultative Methylotroph). The bioprimed seeds were assessed for their physiological attributes and anatomical and biochemical changes. Compared with the control, biopriming with 2% Fish fermented extract significantly improved the germination rate (19.4%), germination energy (40.9%), root length (44.4%), shoot length (24.4%) and seedling vigour index (68.1%). This increase was attributed to the nutritional and bioactive compounds in the fish by-products, which promoted root development and nutrient absorption. The biochemical parameters revealed increased ?-amylase activity, protein, and amino acid content in the bioprimed seeds. Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed beneficial root volatile compounds in the roots of treated seeds. An evaluation of the plant growth-promoting activities of the biopriming agents revealed the superiority of fish-fermented extract over other bioagents. Therefore, this method promotes sustainable agriculture and improved crop productivity, providing a scientific basis for biopriming as a viable alternative to conventional seed treatments.

Transcription factor SlSTOP1 regulates Small Auxin-Up RNA Genes for tomato root elongation under aluminum stress.

Aluminum (Al) stress, a prevalent constraint in acidic soils, inhibits plant growth by inhibiting root elongation through restricted cell expansion. The molecular mechanisms of Al-induced root inhibition, however, are not fully understood. This study aimed to elucidate the role of Small Auxin-up RNAs (SlSAURs), which function downstream of the key Al stress-responsive transcription factor SENSITIVE TO PROTON RHIZOTOXICITY 1 (SlSTOP1) and its enhancer STOP1-INTERACTING ZINC-FINGER PROTEIN 1 (SlSZP1), in modulating root elongation under Al stress in tomato (Solanum lycopersicum). Our findings demonstrated that tomato lines with knocked out SlSAURs exhibited shorter root lengths when subjected to Al stress. Further investigation into the underlying mechanisms revealed that SlSAURs interact with Type 2C Protein Phosphatases (SlPP2Cs), specifically D-clade Type 2C Protein Phosphatases (SlPP2C.Ds). This interaction was pivotal as it suppresses the phosphatase activity, leading to the degradation of SlPP2C.D's inhibitory effect on plasma membrane H+-ATPase. Consequently, this promoted cell expansion and root elongation under Al stress. These findings increase our understanding of the molecular mechanisms by which Al ions modulate root elongation. The discovery of the SlSAUR-SlPP2C.D interaction and its impact on H+-ATPase activity also provides a perspective on the adaptive strategies employed by plants to cope with Al toxicity, which may lead to the development of tomato cultivars with enhanced Al stress tolerance, thereby improving crop productivity in acidic soils.

Integrated Pest Management: An Update on the Sustainability Approach to Crop Protection.

Integrated Pest Management (IPM) emerged as a pest control framework promoting sustainable intensification of agriculture, by adopting a combined strategy to reduce reliance on chemical pesticides while improving crop productivity and ecosystem health. This critical review synthesizes the most recent advances in IPM research and practice, mostly focusing on studies published within the past five years. The Review discusses the key components of IPM, including cultural practices, biological control, genetic pest control, and targeted pesticide application, with a particular emphasis on the significant advancements made in biological control and targeted pesticide delivery systems. Recent findings highlight the growing importance of genetic control and conservation biological control, which involves the management of agricultural landscapes to promote natural enemy populations. Furthermore, the recent discovery of novel biopesticides, including microbial agents and plant-derived compounds, has expanded the arsenal of tools available for eco-friendly pest management. Substantial progress has recently also been made in the development of targeted pesticide delivery systems, such as nanoemulsions and controlled-release formulations, which can minimize the environmental impact of pesticides while maintaining their efficacy. The Review also analyzes the environmental, economic, and social dimensions of IPM adoption, showcasing its potential to promote biodiversity conservation and ensure food safety. Case studies from various agroecological contexts demonstrate the successful implementation of IPM programs, highlighting the importance of participatory approaches and effective knowledge exchange among stakeholders. The Review also identifies the main challenges and opportunities for the widespread adoption of IPM, including the need for transdisciplinary research, capacity building, and policy support. In conclusion, this critical review discusses the essential role of IPM components in achieving the sustainable intensification of agriculture, as it seeks to optimize crop production while minimizing adverse environmental impacts and enhancing the resilience of agricultural systems to global challenges such as climate change and biodiversity loss.

Agricultural nanotechnology for a safe and sustainable future: current status, challenges, and beyond.

Nanotechnology, which involves manipulating matter at the atomic and molecular scales to produce structures and devices ranging from 1 to 100 nm, is increasingly being applied in agriculture. Nanoscale materials possess distinct optical, electrochemical, and mechanical properties that enable the smart, targeted delivery of pesticides, fertilizers, and genetic materials to plants, as well as rapid sensing and on-site monitoring of plant health, soil fertility, and water quality in a digital format. This review explores the application of nanotechnology in agriculture, examining the challenges and benefits related to all aspects of crop production, with a particular focus on regulatory issues. Key findings indicate that nanotechnology can improve crop production and reduce the environmental footprint of agriculture through precise input management. However, several critical issues need to be addressed, including the limited knowledge of the long-term environmental impacts associated with agricultural nanotechnology and the ambiguity of current regulations. This underscores the need for further research to elucidate its impact on soil, water, and environmental and human health, to inform evidence-based regulations. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Emerging Trends in Plant Growth Regulators: Innovations and Applications

The review offers a comprehensive and illuminating summary of the most recent developments in the area of plant growth regulators (PGRs). The purpose of this research is to investigate the rapid advancements and uses of plant growth regulators (PGRs), which are vital instruments for maximizing the growth and productivity of plants. A wide variety of novel PGR compounds and technologies have been created as a result of recent breakthroughs. These new technologies and compounds provide considerable enhancements over the conventional methods. This discusses important discoveries such as innovative synthetic plant growth regulators (PGRs) and biostimulants that improve plant growth, resistance to stress, and crop output. Specifically, it investigates the manner in which these improved PGRs are developed to target certain physiological processes, which ultimately results in treatments that are more precise and successful in crop management. In addition, the study investigates the role that developing technologies play in the implementation of PGRs. These technologies include smart delivery systems and precision agricultural methods, which make it possible to adjust treatments to the individual requirements of plants and the circumstances of their environments. Additionally, the incorporation of PGRs into agricultural operations that are environmentally responsible. It highlights the potential for these advances to help to environmentally friendly agriculture by lowering the dependency on chemical inputs and enhancing the efficiency with which resources are used. The significance of these technological developments in solving global concerns such as climate change and food security is emphasized in the article. The revolutionary effect that new PGR technology have had on contemporary agricultural practices. This article highlights the potential for these technologies to improve crop productivity and promote sustainable agriculture practices by providing a detailed review of current developments and the practical consequences of those trends.

Mitigation strategies for greenhouse gases to ensure food security

Global warming and food insecurity are global concerns, with agriculture being a major contributor to greenhouse gas emissions. Greenhouse gases such as carbon dioxide, nitrous oxide, and methane, from agricultural activities significantly impact climate change. Approximately 24% of global greenhouse gas emissions come from agriculture. Nitrous oxide is 300 times stronger than carbon dioxide and is mainly produced from organic manure and fertilizers. Methane, another potent greenhouse gas, is released during fermentation, manure management, and burning of residues. Carbon dioxide, a major contributor to climate change, is emitted through farming practices, fertilizers, pesticides, and deforestation. Climate change affects food security by directly impacting crop production and indirectly affecting food availability, cost, and supply chains. Hunger rates have been increasing globally, emphasizing the need to control global warming to reduce food insecurity. This review highlights various mitigation strategies for controlling greenhouse gases from agriculture with improved crop productivity. Soil characterization techniques, such as X-ray computed tomography, tracer and chamber-based methods, help to understand the soil composition for greenhouse gas mitigation strategies. Soil amendments, like biochar application can effectively reduce emissions by modifying microbial activity and biogeochemical processes. Controlled irrigation practices, minimum and zero tillage, and efficient nitrogen fertilizer usage also contribute to greenhouse gas mitigation and improves crop productivity. Strategies such as slow release of fertilizers and the use of inhibitors help to increase nitrogen usage efficiency and reduce nitrous oxide emissions. Implementing these strategies globally is crucial for mitigating greenhouse gas emissions, reducing global warming, and ensuring food security.

Effect of Magnetized Irrigation Water and Seeds on the Production of OKRA (Hibiscus esculentus)

Very recently special magnetic devices have been developed and used to magnetize irrigation water and plant seeds. Such magnetic treatments were reported to play a great role in increasing the germination rate of seeds, reducing soil salinity and consequently resulting in increasing yield. The objectives of the study to improve crop productivity by using magnetic technologies. A split plot experimental design was used for growing two varieties of okra (Hibiscus esculentus). The treatments included: non-magnetized water + non magnetized seeds (NMW+NMS), non-magnetized water + magnetized seeds (NMW+MS), magnetized water + non magnetized seeds (MW+NMS), magnetized water + magnetized seeds (MW+MS). Each treatment was replicated three times. The parameters considered included: number of leaves per plant, leaf length, plant height, plants density and yield. There was a significant difference (p <0.05) between treatments, the combination of (MW) and (MS) gave higher of leaves per plant, plant density, and plant height and leaf length. There was a significant difference (p <0.05) in yield between treatments (MW) recorded increasing in yield by 50% as compared to (NMW); (MW) + (MS) recorded increase in yield by 24% as compared to (NMW); (MS) record increasing in yield by 18% as compared to (NMS) when both were irrigated by (NMW), the combination of (MW) and (MS) gave higher yields for both okra varieties (25.5 and 35.5 Kg/ha). It was concluded that magnetizing water may lead to better crop establishment and further improvement could be achieved by magnetizing the seeds.

Recent Advances in Phenazine Natural Products: Biosynthesis and Metabolic Engineering.

Phenazine natural products are a class of nitrogen-containing heterocyclic compounds produced by microorganisms. The tricyclic ring molecules show various chemical structures and extensive pharmacological activities, such as antimicrobial, anticancer, antiparasitic, anti-inflammatory, and insecticidal activities, with low toxicity to the environment. Since phenazine-1-carboxylic acid has been developed as a registered biopesticide, the application of phenazine natural products will be promising in the field of agriculture pathogenic fungi control based on broad-spectrum antifungal activity, minimal toxicity to the environment, and improvement of crop production. Currently, there are still plenty of intriguing hidden biosynthetic pathways of phenazine natural products to be discovered, and the titer of naturally occurring phenazine natural products is insufficient for agricultural applications. In this review, we spotlight the progress regarding biosynthesis and metabolic engineering research of phenazine natural products in the past decade. The review provides useful insights concerning phenazine natural products production and more clues on new phenazine derivatives biosynthesis.

Effect of Integrated Weed Management Practices in Rice (Oryza sativa) Under SRI

An experimental study was conducted at the Annamalai University Experimental Farm in Cuddalore District, Tamil Nadu, between February and May 2023. The objective of this work is to chemically manage the developing weeds in rice during vegetative development using the System of Rice Intensification (SRI) technology. Additionally, the study aims to identify the weed flora in the experimental field. In this study, a Randomised Block Design with three replications was employed. The experimental treatments comprised optimal combinations of chemical and mechanical techniques for weed management. The experiment was structured in a randomised block design, consisting of seven treatments, and subsequently reproduced three times. Among the several treatments, the application of Penoxsulam + Butachlor @ 2L/ha (PE) on 3 days after transplantation (DAT) for rice, and the use of Conoweeder twice on 25 and 35 DAT (rice), effectively decreased weed density and thus reduced weed biomass. This led to an improvement in crop development and production. The greater plant height, drying matter potential (DMP), and grain yield seen with the application of Penoxsulam + Butachlor @ 2L/ha (PE) on 3 days after transplantation (DAT), and Conoweeder twice on 25 and 35 DAT, may be attributed to the effective management of a weed-free environment, especially during critical growth phases of the crop. Suppression of agricultural weed competition facilitated increased growth and development of rice crop, resulting in elevated grain and straw yield.

A History of Community Based Initiatives that Led to Crop Improvement and Protection in the Canadian Prairies

From the early days of agricultural production in the 1800s through to the present day, farmers, agronomists, and other motivated people have worked to improve crop production through pest management and surveillance, selection of crop genotypes and agronomic innovations such as reduced and zero-tillage. These essential contributions also helped raise awareness of the practical problems that farmers have faced, of the potential solutions to those problems, and the problems that remain to be solved. In many cases, farmers have organized their efforts to support research to address agricultural challenges through commodity organizations who actively fund research, raise awareness of science, and encourage participation in activities such as pest monitoring and on-farm research trials. The future of a biovigilance approach to crop production depends on the continued participation of agricultural community members.

Multi-metal tolerant Porostereum umbrinoalutaceum isolated from Pleurotus ostreatus fruit body showing root colonization, growth promotion of chilli (Capsicum annuum L.) plant and antagonism against fungal pathogens.

Mushroom associated microbes could be utilized to improve crop productivity providing nutrients, plant growth promoting substances, production of hydrolytic enzymes and protecting plant from biotic and abiotic stress. An endophyte designated as KUFC101 was isolated from fruit body of Pleurotus ostreatus and identified as Porostereum umbrinoalutaceum based on nuclear-rRNA gene sequence analysis. Growth in different culture media, metal tolerance, biochemical characterization and effect on chilli plant growth promotion were studied. The isolate showed best growth in Malt extract medium and least growth in synthetic media. It could tolerate toxic metals (Mg, Ca, Fe, Cu, Mn, Zn and Cd each at 100ppm concentration). It produced amylase, cellulase, chitinase, pectinase, catecholate type of siderophore and indole acetic acid, and inhibited growth of Alternaria solani and Penicillium citrinum. It could colonize in the rhizosphere of chilli plant and influence growth of chilli plant by improving biomass and metabolite content. Porostereum umbrinoalutaceum KUFC101 could be utilized in formulation of biofertiliser under sustainable agricultural system.