Improved Pulse Sequence Research Articles

An improved method to accelerate the acquisition efficiency of the T1-T2 spectrum based on the IR-BSSFP-CPMG pulse sequence

The correlation map of the longitudinal relaxation time (T1) and the transverse relaxation time (T2) offers valuable information of different proton contributions and is served as a significant data for identifying, quantifying and characterizing complex relaxation components in unconventional reservoirs. However, the acquisition speed and spectral resolution for existing pulse sequences cannot be guaranteed simultaneously. We designed an improved pulse sequence which integrates the inversion recovery-balanced steady-state free precession (IR-BSSFP) sequence and the Carr-Purcell-Meiboom-Gill (CPMG) sequence, allowing for the measurement of two-dimensional T1-T2 spectrums. The IR-BSSFP sequence adopts a low refocusing angle and reduced flip angle pulse transmission power, eliminating the need for complete polarization. Moreover, the parameters of the improved pulse sequence such as the value and the number of the flip angle, the repetition time are discussed by numerical simulations. The result showed that the new pulse sequence achieves high-precision measurement performance, and the T1-T2 spectrum can be achieved within a few seconds.

Improved frequency shift compensation technique and pulse sequence for multi-loop atom interference experiments

With the rapid development of atom interference technology, multi-loop atom interferometers are widely used in the high-precision measurement of various physical constants and testing of various gravity-related effects. However, in ground-based multi-loop atom interference experiments, the systematic error contribution by classical effects is an important factor that affects the experimental measurement accuracy and gravitational effect detection. Based on this, we used the atomic wave-function evolution-phase accumulation method to provide a high-order interference phase of multi-loop atom interferometers in an inhomogeneous gravitational field containing Earth’s rotation. Furthermore, we propose a new scheme that combines optimised frequency-shift compensation technology with an improved pulse sequence to eliminate systematic errors due to the gravity gradient, Earth’s rotation, and their coupling effect with the pulse duration, as well as the coupling effect of laser detuning with the pulse duration. This study lays a theoretical foundation for experiments on multi-loop atom interferometers with higher precision.

Improved Raman pulse sequence for rotation measurements with atom interferometers

Since the double-loop atom interferometer consisting of Raman four-pulse sequences can eliminate the dependence of the interferometric phase shift on the atomic initial velocity, it is widely used for rotation measurements. However, our research shows that by coupling itself with both the atomic initial velocity and the laser detuning, the short pulse duration can contribute to unknown systematic errors within the current instrument sensitivity. The coupling of the pulse duration with the laser detuning inside the device is verified experimentally and presented in this work. An improved pulse sequence design is developed to eliminate these systematic errors and improve the precision of the rotation measurements.

A Novel Discontinuous PWM Strategy to Control Neutral Point Voltage for Neutral Point Clamped Three-Level Inverter With Improved PWM Sequence

In order to reduce switching loss of neutral point clamped three-level inverter (NPC TLI), generally discontinuous pulsewidth modulation (DPWM) is used. But it can result in dc offset and ac ripple on neutral point (NP) voltage. So a novel pulse sequence DPWM (NPSDPWM) is proposed to reduce switching loss and control NP voltage simultaneously in this paper. NP voltage is controlled by choosing proper clamping modes. To avoid unexpected switching action during changing clamping mode, an improved pulse sequence is also presented. The switching loss and NP voltage ripple of NPSDPWM, traditional and proposed DPWM in previous literature are compared, respectively. The experimental results show that NPSDPWM has well NP voltage control ability and the switching losses are reduced effectively.

Efficiency Improvement of Time Modulated Array With Reconfigurable Power Divider/Combiner

Time modulated array (TMA) suffers from relatively low efficiencies due to the power absorption in switches during off state. In this paper, we present the design of a novel TMA by using a reconfigurable power divider/combiner (RPDC) for improving the array’s efficiency, where the power absorbed during off state can evenly be transferred to other array elements during on state. An improved pulse sequence strategy is developed to control the radiation pattern of the TMA with RPDC. The impact of the RPDC on radiation features of the TMA is further discussed. To experimentally verify the feasibility of the proposed design, a six-element TMA with RPDC is constructed and characterized. Compared with a six-element conventional TMA with single-pole single-throw switches, the TMA with RPDC shows a significant enhancement of gain by 0.98, 1.27, and 1.65 dB when the sidelobe levels are −20, −25, and −30 dB, respectively.

An improved pulse sequence and inversion algorithm of [formula omitted] spectrum

The nuclear magnetic resonance transversal relaxation time is widely applied in geological prospecting, both in laboratory and downhole environments. However, current methods used for data acquisition and inversion should be reformed to characterize geological samples with complicated relaxation components and pore size distributions, such as samples of tight oil, gas shale, and carbonate. We present an improved pulse sequence to collect transversal relaxation signals based on the CPMG (Carr, Purcell, Meiboom, and Gill) pulse sequence. The echo spacing is not constant but varies in different windows, depending on prior knowledge or customer requirements. We use the entropy based truncated singular value decomposition (TSVD) to compress the ill-posed matrix and discard small singular values which cause the inversion instability. A hybrid algorithm combining the iterative TSVD and a simultaneous iterative reconstruction technique is implemented to reach the global convergence and stability of the inversion. Numerical simulations indicate that the improved pulse sequence leads to the same result as CPMG, but with lower echo numbers and computational time. The proposed method is a promising technique for geophysical prospecting and other related fields in future.

An intraresidual i(HCA)CO(CA)NH experiment for the assignment of main-chain resonances in 15N, 13C labeled proteins

An improved pulse sequence, intraresidual i(HCA)CO(CA)NH, is described for establishing solely (13)C'(i), (15)N(i), (1)HN(i) connectivities in uniformly 15N/13C-labeled proteins. In comparison to the "out-and-back" style intra-HN(CA)CO experiment, the new pulse sequence offers at least two-fold higher experimental resolution in the (13)C' dimension and on average 1.6 times higher sensitivity especially for residues in alpha-helices. Performance of the new experiment was tested on a small globular protein ubiquitin and an intrinsically unfolded 110-residue cancer/testis antigen CT16/PAGE5. Use of intraresidual i(HCA)CO(CA)NH experiment in combination with the established HNCO experiment was crucial for the assignment of highly disordered CT16.

Design of cosine modulated very selective suppression pulses for MR spectroscopic imaging at 3T

The advantages of using a 3 Tesla (T) scanner for MR spectroscopic imaging (MRSI) of brain tissue include improved spectral resolution and increased sensitivity. Very selective saturation (VSS) pulses are important for maximizing selectivity for PRESS MRSI and minimizing chemical shift misregistration by saturating signals from outside the selected region. Although three-dimensional (3D) PRESS MRSI is able to provide excellent quality metabolic data for patients with brain tumors and has been shown to be important for defining tumor burden, the method is currently limited by how much of the anatomic lesion can be covered within a single examination. In this study we designed and implemented cosine modulated VSS pulses that were optimized for 3T MRSI acquisitions. This provided improved coverage and suppression of unwanted lipid signals with a smaller number of pulses. The use of the improved pulse sequence was validated in volunteer studies, and in clinical 3D MRSI exams of brain tumors.

Linear phase slope in pulse design: Application to coherence transfer

Using optimal control methods, robust broadband excitation pulses can be designed with a defined linear phase dispersion. Applications include increased bandwidth for a given pulse length compared to equivalent pulses requiring no phase correction, selective pulses, and pulses that mitigate the effects of relaxation. This also makes it possible to create pulses that are equivalent to ideal hard pulses followed by an effective evolution period. For example, in applications, where the excitation pulse is followed by a constant delay, e.g. for the evolution of heteronuclear couplings, part of the pulse duration can be absorbed in existing delays, significantly reducing the time overhead of long, highly robust pulses. We refer to the class of such excitation pulses with a defined linear phase dispersion as ICEBERG pulses (Inherent Coherence Evolution optimized Broadband Excitation Resulting in constant phase Gradients). A systematic study of the dependence of the excitation efficiency on the phase dispersion of the excitation pulses is presented, which reveals surprising opportunities for improved pulse sequence performance.

Improved pulse schemes for separated local field spectroscopy for static and spinning samples

An improved pulse sequence for SLF experiments based on the magic sandwich (MS) scheme for homo-nuclear dipolar decoupling is proposed. The sequence incorporates a double MS, both on I and S spins and has been named as EXE-MS2. The proposed scheme which has a scaling factor of 1 is observed to be free from low intensity artifacts and provides better line-widths particularly for S spins labeled at multiple sites. The pulse sequence which has been applied on static oriented samples incorporates the EXE scheme where direct polarization of the S spin in the B 0 field is utilized in the place of polarization inversion and is observed to perform well without any loss of sensitivity while ensuring considerable reduction in rf power input into the sample. The EXE scheme has also been tested for solid samples under MAS.

A Review of Technical Advances in Interventional Magnetic Resonance Imaging 1

Initial research in the development of interventional magnetic resonance (MR) imaging in the late 1980s and early to mid-1990s focused on pulse sequences, devices, and clinical applications. This focus was largely a result of the limited number of areas in which the academic research community leading the development could provide innovation on the MR systems of the time. However, during the past decade, computational power, higher bandwidth graphical displays, faster computer networks, improved pulse sequence architectures, and improved technical specifications have accelerated the pace of development on modern MR systems. Today, it is the combination of multiple system factors that are enabling the future of interventional MR. These developments, their impact on the field, and newly emerging applications are described.

Improving pulse sequences for 3D DOSY: COSY-IDOSY

An improved pulse sequence for measuring diffusion-ordered COSY spectra is achieved by incorporating the diffusion encoding directly into the evolution and detection periods of a gradient-enhanced COSY sequence, giving improved sensitivity and a 32-fold reduction in minimum experiment time.

Improving pulse sequences for 3D diffusion-ordered NMR spectroscopy: 2DJ-IDOSY.

An improved pulse sequence for the 3D DOSY experiment 2DJ-DOSY, using diffusion encoding internal to the parent 2DJ spectroscopy sequence (2DJ-IDOSY), is presented. The diffusion-encoding pulses are used to enforce the desired coherence transfer pathway, reducing the minimum experimental time by at least a factor of 4, as compared to existing techniques, and approximately doubling the signal-to-noise ratio for small molecules. The new sequence is demonstrated on a simple mixture and on a complex sample with a high dynamic range (port wine). The principle of internal diffusion encoding can be applied with profit to a range of other 3D DOSY experiments.

Suppression of dephasing of optically trapped atoms

The decay in the hyperfine coherence of optically trapped atoms due to interactions with the environment, is only partly suppressed by ``echo'' spectroscopy [M. F. Andersen et al. Phys. Rev. Lett. 90, 023001 (2003)], primarily due to dynamical (time-dependent) dephasing mechanisms. We demonstrate here an improved pulse sequence, for which the decay of coherence is reduced by a factor of $2.5$ beyond the reduction offered by the ``echo'' spectroscopy. This reduction occurs when each dark period in-between pulses is shorter than the time scale over which substantial dephasing develops. The coherence time is then limited by mixing to other vibrational levels in the trap and, to a lesser extent, the lifetime of the internal states of the atoms.

Improved measurement of (3)J(H(alpha)(i),N(i+1)) coupling constants in H(2)O dissolved proteins.

A modification to the recently proposed alpha/beta-HN(CO)CA-J TROSY pulse sequence (P. Permi et al., J. Magn. Reson. 146, 255-259 (2000)) makes it possible to determine (3)J(H(alpha)(i), N(i+1)) coupling constants from a single E.COSY-type cross-peak pattern rather than from two (1)H(alpha) spin-state-edited subspectra. Advantages are increased (15)N resolution, critical to extracting accurate (1)H(alpha)-(15)N coupling constants, and minimized differential relaxation due to nested (13)C(alpha) and (15)N evolution periods. Application of the improved pulse sequence to Desulfovibrio vulgaris flavodoxin results in (3)J(H(alpha)(i), N(i+1)) values being systematically larger than those obtained with the original scheme. Parametrization of the coupling dependence on the protein backbone torsion angle psi yields the Karplus relation (3)J(H(alpha)(i), N(i+1))=-1.00 cos(2)(psi-120 degrees )+0.65 cos(psi-120 degrees )-0.15 Hz, with a residual root-mean-square difference of 0.13 Hz between measured and back-calculated coupling constants. The curve compares with data derived from ubiquitin (A. C. Wang and A. Bax, J. Am. Chem. Soc. 117, 1810-1813 (1995)), although spanning a slightly larger range of J values in flavodoxin. The orientation of the Ala39/Ser40 peptide link, forming a type-II beta-turn in flavodoxin, is twisted against X-ray-derived torsions by approximately 10 degrees in the NMR structure as evident from the analysis of straight phi- and psi-related (3)J coupling constants. The remaining deviation of some experimental values from the prediction is likely to be due to strong hydrogen bonding, substituent effects, or the additional dependence on the adjacent torsions straight phi.

Dynamics of beta-CH and beta-CH2 Groups of Amino Acid Side Chains in Proteins.

The dynamics of amino acid side chains of uniformly 13C/15N-enriched ribonuclease T1 (RNase T1) have been investigated. Heteronuclear longitudinal relaxation rates, 1H/13C NOEs, and transverse cross-correlated cross-relaxation rates between the Sx and the SxIz1Iz2 operators (SIIS cross relaxation) [Ernst and Ernst (1994) J. Magn. Reson., A110, 202-213] have been determined in this study. New pulse sequences for measuring the longitudinal relaxation time and the heteronuclear NOE of aliphatic side chain carbon nuclei were developed using the CCONH type of magnetization transfer and 1HN detection. In addition, an improved pulse sequence for the determination of the SIIS cross relaxation is presented. For the analysis of the relaxation rates, the model of restricted rotational diffusion around the chi1 dihedral angle has been applied [London and Avitabile (1978) J. Am. Chem. Soc., 100, 7159-7165]. These techniques were used in order to describe the side chain dynamics of the small globular protein RNase T1 (104 amino acids, MW about 11 kDa). Qualitative values of microdynamical parameters were obtained for 73 out of 85 amino acid side chains (glycine and alanine residues excepted) whereas more quantitative values were derived for 67 beta-CH and beta-CH2 groups.

In situ nuclear magnetic resonance study of defect dynamics during deformation of materials

Nuclear magnetic resonance techniques can be used to monitor in situ the dynamical behaviour of point and line defects in materials during deformation. These techniques are non-destructive and non-invasive. We report here the atomic transport, in particular the enhanced diffusion during deformation by evaluating the spin lattice relaxation time in the rotating frame, Tip, in pure NaCI single crystals as a function of temperature (from ambient to about 900 K) and strain-rate (to ~ 1.0 s -1) in situ during deformation. The strain-induced excess vacancy concentration increased with the strain-rate while in situ annealing of these excess defects is noted at high temperatures. Contributions due to phonons or paramagnetic impurities dominated at lower temperatures in the undeformed material. During deformation, however, the dislocation contribution became predominant at these low temperatures. The dislocation jump distances were noted to decrease with increase in temperature leading to a reduced contribution to the overall spin relaxation as temperature is increased. Similar tests with an improved pulse sequence (CUT-sequence), performed on ultra-pure NaCI and NaF single crystals revealed slightly different results; however, strain-enhanced vacancy concentrations were observed. The applicability of these techniques to metallic systems will be outlined taking thin aluminium foils as an example.

Multiple slice FLASH imaging: An improved pulse sequence for contrast enhanced MR brain studies

A multisclice gradient echo sequence (FLASH) was compared with a conventional spin-echo (SE) technique with regard to its value for contrast enhanced brain studies. In 50 patients with contrast enhancing intracranial lesions, MR studies (0.5 Tesla MR tomograph) were performed with SE images (SE 400/30; four images/3.4 min) and FLASH scans (FLASH 315/14, 90 degrees; 15 images/1.4 min) before and after Gd-DTPA. Based on visual and quantitative assessment diagnostic results of postcontrast SE- and FLASH images were equivalent with respect to contrast enhancement, lesion/brain-contrast, lesion/edema-contrast, and lesion delineation. Although image quality generally was excellent on postcontrast FLASH images, susceptibility artifacts were more severe on FLASH scans than on SE images. However, with the exception of postoperative patients with artifacts due to metal remains, diagnostic information was not decreased by artifacts on postcontrast FLASH images. In conclusion, because of the clearly higher efficiency of the multisclice FLASH technique, this pulse sequence offers the opportunity to speed up contrast enhanced brain imaging.