Background About 10% of all strokes occur in hospitalized patients. The goal of this work was to evaluate the knowledge of stroke signs and to determine predictors of that knowledge among inpatient staff at an academic medical center. Methods Stroke education was the topic of a mandatory in-service for all adult inpatient medical, surgical, and ICU nursing unit clinical staff; including nurses, techs, and aides. The staff members anonymously completed an optional web-survey which included free text responses for stroke signs and symptoms, along with additional multiple choice questions regarding experience and training. The primary outcome was stroke knowledge which was defined as correct naming of 2 or more stroke warning signs or symptoms. Logistic regression was used to determine predictors of the primary outcome. Results The survey was offered to 1,593 staff members and 875 (55%) completed the survey. Eighty-seven percent of inpatient staff members correctly identified 2 or more stroke warning signs or symptoms while 31% identified 3 stroke warning signs or symptoms. Individual level predictors of stroke knowledge are shown in the Table. Greater self-reported confidence in identifying stroke symptoms and higher ratings for the importance of rapid identification of stroke symptoms were associated with stroke knowledge. Clinical experience, educational experience, work location, and personal knowledge of a stroke patient were not associated with stroke knowledge. Conclusion More than 80% of adult clinical inpatient staff members have knowledge of two or more stroke signs and symptoms. Future nursing education should emphasize the importance of rapid identification of stroke signs and symptoms and increasing confidence in knowledge of stroke symptoms.