The purpose of this study was to explore the effects of mixed emotional stimuli on associative memory. Emotional word pairs were chosen as the materials for the experiment, and the classical associative reconsideration paradigm was used to explore the effects of mixed emotional stimuli on associative memory under the condition of controlled arousal. Subjects were asked to learn different types of emotional word pairs in the learning phase, and associative recognition judgments were made on the word pairs in the recognition phase. It was found that the associative recognition scores (Pr) for mixed-emotion stimuli, positive-emotion stimuli, and negative-emotion stimuli were all significantly smaller than those for the neutral condition. The difference in associative recognition scores between mixed-emotion stimuli and positive-emotion stimuli was not significant. The association recognition scores for both mixed-emotion stimuli and positive-emotion stimuli were significantly higher than the association recognition scores for negative-emotion stimuli. The results suggest that mixed-emotion stimuli, positive-emotion stimuli, and negative-emotion stimuli impair associative memory compared to the neutral condition. Negative emotional stimuli impaired associative memory to the greatest extent, followed by mixed emotional stimuli and positive emotional stimuli.