Soil quality offers a holistic approach for understanding the relationships between soil’s biological, chemical, and physical properties, which is crucial for sustainable land use and the management of non-renewable soil resources. This study evaluates the impact of land use on a set of 23 soil quality indicators (SQIs) across 5 land uses of the Mediterranean agro-ecosystems: forest, olive groves, wheat fields, a corn/wheat crop rotation system, and pasture. Seasonal soil sampling was carried out over two consecutive years in three conventionally managed fields representing each land use type. For each sampling, physicals SQIs (soil moisture, porosity-Vp-, bulck density-BD-, water holding capacity-WHC-, clay, silt, sand), chemical SQIs (organic carbon-Corg-, total Nitrogen-TN-, C/N, PH, electrical conductivity-EC-, ammonium-NH4-N-, nitrate-NO3-N- and available nitrogen-Nmin-), and biological SQIs (soil microbial biomass C-Cmic- and N-Nmic-, Cmic/Nmic, Cmic/Corg, Nmic/TN, active carbon—Cact-, Cact/Corg) were evaluated. Through multivariate analysis, five key soil quality factors—organic matter, microbial biomass, nutrients, C/N ratio, and compaction—were identified as indicators of soil quality changes due to land use, explaining 82.9% of the total variability in the data. Discriminant analysis identified organic matter and the C/N factors as particularly sensitive indicators of soil quality changes, reflecting the quantity and quality of soil organic matter, incorporating 87.8% of the SQIs information resulting from the 23 indicators. ΤΝ, accounting for 84% of the information on the organic matter factor, emerges as a key indicator for predicting significant changes in soil quality due to land use or management practices. The TN and C/N proposed indicators offer a simplified yet effective means of assessing soil resource sustainability in the Mediterranean agroecosystems, providing practical tools for monitoring and managing soil quality.
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