The role of natural fibers on the impact fatigue strength of bonded single lap joints (SLJs) has been experimentally investigated. The use of natural fiber offers several advantages, including the potential to recycle natural resources and the utilization of sustainable materials. Four types of natural date palm tree fibers (DPTFs) including Rachis, Petiole, Bunch, and Mesh were used to improve the impact fatigue life and the residual static strength of joints after enduring repetitive low energy impacts. The fibers were treated by 6 wt% of NaOH solution and cut manually to short size (0.5–2 mm) and were used by weight ratios of 2 and 5 %. The results show that the addition of DPTFs to the adhesive layer significantly improves the impact fatigue strength of the reinforced joints compared to neat adhesive SLJs. The joints reinforced by 5 wt% and 2 wt% of Mesh improved the impact fatigue life of the joints by 197 % and 87 %, respectively. The impact fatigue life of the joints modified by 5 wt% of fibers was more than the joints reinforced by 2 wt%. Fractography analysis also showed that DPTFs make the crack propagation more stable and increase the strength of the tested joints on different conditions.