COL11A2 Methylation as a Biomarker for Radiosensitivity and Microenvironment Remodelling in Oral Squamous Cell Carcinoma.

The Role of Interferon-γ-Induced Granzyme B in Enhancing Antitumour Immune Responses in Oral Squamous Cell Carcinoma.

Identification and experimental confirmation of TBC1D10C as a shared transcriptional link between abdominal aortic aneurysm and major depressive disorder.

Single-cell analysis of TIGD genes in hepatocellular carcinoma: Prognostic value and functional characterization.

Integrative bioinformatics identifies NSCLC biomarkers associated with LPS metabolism and circadian disruption.

Electrochemical sensors used for identify autophagy-related hub genes and their correlation with immune infiltration in nasopharyngeal carcinoma: A bioinformatics analysis combined with experimental validation

Identification of mitochondrial dysfunction-related biomarkers and immune infiltration in liver ischemia-reperfusion injury via integrated bioinformatics and machine learning.

Idiopathic Pulmonary Fibrosis (IPF) is a progressive and fatal interstitial lung disease characterized by excessive extracellular matrix (ECM) deposition and tissue stiffening. Matrix stiffness is a key driver of fibrosis, yet diagnostic biomarkers directly linked to this physical property are lacking. This study aimed to identify robust matrix stiffness-related diagnostic biomarkers and potential therapeutic targets for IPF using an integrated machine learning approach. Gene expression profiles were obtained from the GEO database (Training set: GSE33566; Validation set: GSE93606). Differentially expressed genes (DEGs) were intersected with a matrix stiffness-related gene set. Three machine learning algorithms (SVM-RFE, LASSO, and Naive Bayes) were employed to screen diagnostic feature genes. A diagnostic nomogram was constructed and evaluated. Functional enrichment (GO/KEGG/GSEA), immune infiltration (ssGSEA), and molecular docking analyses were performed to explore biological functions and predict therapeutic drugs. Eighteen matrix stiffness-related DEGs were identified. Through machine learning screening, GSN and ARG1 were determined as robust key genes, exhibiting high diagnostic accuracy (AUC>0.7) in both training and validation cohorts. Functional analysis revealed that GSN is involved in actin cytoskeleton regulation, while ARG1 participates in immune response modulation. Both genes showed strong positive correlations with the infiltration of macrophages and neutrophils. Furthermore, molecular docking identified RA-2 as a potential therapeutic agent targeting ARG1 with high binding affinity (-9.2kcal/mol). We identified GSN and ARG1 as novel matrix stiffness-related diagnostic biomarkers for IPF, linking mechanotransduction to immune microenvironment remodeling. The diagnostic nomogram offers high clinical predictive value, and RA-2 emerged as a putative ARG1-targeting compound with favorable docking energy and warrants further experimental validation as a potential antifibrotic agent.

DLAT suppresses tumor progression and modulates treatment response in colorectal cancer: Insights from a metabolic prognostic model.

Exosome-related lactylation gene signature defines diagnostic biomarkers of periodontitis through integrative bulk and single-cell transcriptomics.

NIR-II imaging-guided nanoplatform for synergistic mitochondria-targeted pyroptosis and macrophage reprogramming immunotherapy.

Trichosanthes kirilowii extract alleviated liver fibrosis and acute myocardial infarction by inhibiting VCAN to restrain monocyte senescence: Insights from bioinformatic analyses and experimental validation.

The role of stromal cell immunobiology in understanding and managing Sjögren's disease.

Immune landscape in NOD.H-2h4 mouse model thyroid revealed by single-cell RNA sequencing.

Mechanisms of bisphenols-induced cervical cancer: A multidimensional bioinformatics analysis.

An optimized method for establishing experimental rat periodontitis using "double-ligature" technique.

Improved somatic mutation profiling in small-cell lung cancer using RNA-sequencing and the T2T-CHM13 genome reference.

Tumor cell derived CCL20 exacerbates the immunosuppressive microenvironment by recruiting CCR6+ Tregs in pancreatic cancer.

Targeting FN1 to overcome gemcitabine resistance in gallbladder cancer: Mechanistic insights and an iRGD-modified PEG-PLGA nanoparticle delivery strategy.

Placental anti-angiogenic and inflammatory markers and postpartum cardiovascular risk following preeclampsia.