Levels Of Immune Complexes Research Articles

Comparative immunohematological profile of <i>Pelophylax ridibundus</i> and <i>P. lessonae</i> (Amphibia: Ranidae) infected with hemoparasites

The direction of adaptive reactions of healthy and Hepatozoon spp.-infected green frogs from water bodies of the Nizhni Novgorod region with various hydrochemical conditions was studied. During the observation period, infection of pool frogs and an increase in the total population indicators of infection of male and female marsh frogs with Hepatozoon spp. were revealed. Hypertrophy of the morphometric parameters of infected blood erythrocytes compared with healthy cells was revealed in marsh frogs. The content of neutrophil granulocytes in the blood of infected marsh frogs was higher compared to healthy individuals, while the level of immune complexes did not differ. In infected pool frogs, an increase in the level of large immune complexes in the blood serum and the enlargement index was revealed compared to healthy ones against the background of a general decrease in the body’s resistance in conditions of severe pollution of their habitat with heavy metals and sulfates. For marsh frogs, a correlation was established between the proportion of infected erythrocytes and the content of myelocytes and young forms of neutrophils in the blood; while for pool frogs – with the content of neutrophils and the enlargement index of immune complexes. The method of multifactorial dispersion analysis revealed a significant influence of such factors as gender and the interaction of gender/infection with hemoparasites on the immune status of amphibians.

Association Between Cytokeratin 19-Specific IgG and Neutrophil Activation in Asthma.

Patients with non-eosinophilic asthma (NEA) are less responsive to anti-inflammatory drugs and suffer from frequent asthma exacerbations. The pathogenic mechanism of NEA is not fully understood; however, the roles of monocytes and autoimmune mechanisms targeting airway epithelial cell (AEC) antigens have been proposed. The effects of monocyte extracellular traps (MoETs) on cytokeratin 19 (CK19) production in AECs, as well as the impact of CK19-specific immunoglobulin (Ig) G on neutrophil and monocyte activation, were investigated both in vivo and in vitro. Sixty asthmatic patients and 15 healthy controls (HCs) were enrolled, and the levels of serum immune complexes containing CK19-specific IgG and neutrophil extracellular trap (NET)-specific IgG were measured using enzyme-linked immunoassay. MoETs induced CK19 and CK19-specific IgG production. Furthermore, the levels of serum CK19-specific IgG were significantly higher in the NEA group than in the eosinophilic asthma group. Among patients with NEA, asthmatics with high levels of CK19-specific IgG had higher levels of myeloperoxidase and NET-specific IgG than those with low levels of CK19-specific IgG (P = 0.020 and P = 0.017; respectively). Moreover, the immune complexes from asthmatics with high CK19-specific IgG enhanced NET formation and reactive oxygen species production (neutrophil activation), which were suppressed by N-acetylcysteine and anti-CD16 antibody treatment. These findings suggest that circulating CK19 and CK19-specific IgG may contribute to NET formation, leading to airway inflammation and steroid resistance in NEA.

Nipocalimab, an anti-FcRn monoclonal antibody, in participants with moderate to severe active rheumatoid arthritis and inadequate response or intolerance to anti-TNF therapy: results from the phase 2a IRIS-RA study

ObjectivesTo investigate the efficacy, safety, pharmacokinetics and pharmacodynamics of nipocalimab in participants with moderate to severe active rheumatoid arthritis (RA) and inadequate response or intolerance to ≥1 antitumour necrosis factor...

#2314 Circulating immune complexes concentration and complement activation in sensitized kidney transplant recipients

Abstract Background and Aims Chronic antibody mediated rejection of the kidney transplant is the most common cause of graft loss in the late posttransplant period. Crucial in this process seems to be the role of classical pathway complement activation due to the formation of immune complexes. The formation of immune complexes is a benign process of a normally functioning immune system. In kidney transplant recipients (KTRs), the production of donor specific IgG/IgM antibodies (DSAs) against donor antigens (MHC molecules, ABO blood group antigens) leads to the attachment of immune complexes to cell membranes and subsequent tissue damage through complement activation. The attachment of C1q complement fragment to the immune complexes holds an important part in this process. This study aims to determine the levels of immune complexes as well as classical pathway complement activation in sensitized kidney transplant recipients. Method In this cross-sectional study 3 groups of KTRs were compared as to the levels of immunocomplexes concentration and classical pathway complement activation. Fourteen KTRs with preformed or de novo donor specific antibodies were compared to 28 kidney transplant recipients with no DSAs and to 22 subjects with no history of chronic kidney disease (controls). We used MicroVue Complement CIC-C1q EIA that is designed to detect immunocomplexes that bind to C1q in human serum or plasma. In order to estimate total classical pathway complement activation in serum, we used MicroVue Complement CH50 Eq EIA. Results Kidney function (eGFR) of KTRs with and without DSAs was similar (53.1 ± vs. 60.8 ± ml/min/1.73 m2, p = 0.54) but lower in comparison to controls (106.4 ml/min/1.73 m2, p < 0.001). We found a significantly higher concentration of C1q-immunocomplexes in the serum of KTRs with donor specific antibodies, in comparison to controls, as well as to the group of kidney transplant recipients with no DSAs (6.78 ± 2.7 vs. 4.83 ± 1.9 vs. 5.04 ± 1.2 μgEq/ml respectively, p = 0.0078). Furthermore, there was no significant difference of C1q-immunocomplexes between kidney transplant recipients with no DSAs and controls. Finally, a significant reduction of CH50 levels was observed in KTRs with DSAs as compared to controls and KTRs with no DSAs (39.2 ± 14.9 vs. 70.6 ± 34.3 vs. 67.9 ± 39.9 U/ml respectively, p = 0.003), which suggests higher levels of classical pathway complement activation. CH50 levels in KTRs with DSAs, who were further subcategorized in those with preformed and those with de novo DSAs were similar (39.68 ± 15.6 vs 39.08 ± 16.04 U/ml). Conclusion Kidney transplant recipients with donor specific antibodies have increased serum levels of immune complexes and increased classical pathway complement activation.

Asparaginase-specific basophil recognition and activation predict Asparaginase hypersensitivity in mice.

Asparaginase (ASNase) is a crucial part of acute leukemia treatment, but immune responses to the agent can reduce its effectiveness and increase the risk of relapse. Currently, no reliable and validated biomarker predicts ASNase-induced hypersensitivity reactions during therapy. We aimed to identify predictive biomarkers and determine immune cells responsible for anaphylaxis using a murine model of ASNase hypersensitivity. Our preclinical study uses a murine model to investigate predictive biomarkers of ASNase anaphylaxis, including anti-ASNase antibody responses, immune complex (IC) levels, ASNase-specific binding to leukocytes or basophils, and basophil activation. Our results indicate that mice immunized to ASNase exhibited dynamic IgM, IgG, and IgE antibody responses. The severity of ASNase-induced anaphylaxis was found to be correlated with levels of IgG and IgE, but not IgM. Basophils from immunized mice were able to recognize and activate in response to ASNase ex vivo, and the extent of recognition and activation also correlated with the severity of anaphylaxis observed. Using a multivariable model that included all biomarkers significantly associated with anaphylaxis, independent predictors of ASNase-induced hypersensitivity reactions were found to be ASNase IC levels and ASNase-specific binding to leukocytes or basophils. Consistent with our multivariable analysis, we found that basophil depletion significantly protected mice from ASNase-induced hypersensitivity reactions, supporting that basophils are essential and can be used as a predictive marker of ASNase-induced anaphylaxis. Our study demonstrates the need for using tools that can detect both IC- and IgE-mediated hypersensitivity reactions to mitigate the risk of ASNase-induced hypersensitivity reactions during treatment.

Effect of Telitacicept on Circulating Gd-IgA1 and IgA-Containing Immune Complexes in IgA Nephropathy

IntroductionTelitacicept, a transmembrane activator and cyclophilin ligand (CAML) interactor (TACI) fusion protein targeting B cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL), has proven efficacy in treating Immunoglobulin A (IgA) nephropathy. However, serum biomarkers that could predict the clinical response during the treatment remain unclear. MethodsPlasma samples from 24 participants in the Phase 2 clinical trial were collected at baseline and after 4, 12, and 24 weeks, and 8 participants in the placebo group, 9 in the 160 mg group, and 7 in the 240 mg group. We measured the levels of Galactose deficient-IgA1(Gd-IgA1) , IgA-containing immune complexes, C3a, C5a, and sC5b-9. The association between the changes in these markers and proteinuria reduction was analyzed. ResultsAfter 24 weeks of treatment, Gd-IgA1 decreased by 43.9% (95% CI: 29.8%, 55.1%), IgG-IgA immune complex by 31.7% (14.4%, 45.5%), and poly-IgA immune complex by 41.3% (6.5%, 63.1%) in the 160 mg group; Gd-IgA1 decreased by 50.4% (38.6%, 59.9%), IgG-IgA immune complex decreased by 42.7% (29.5%, 53.4%), and poly-IgA immune complex decreased by 67.2% (48.5%,79.1%) in the 240 mg group. There were no significant changes in the circulatory C3a, C5a, or sC5b-9 levels during telitacicept treatment. Decreases in both plasma Gd-IgA1 and IgG-IgA or poly-IgA immune complexes were associated with proteinuria reduction. In turn, IgG-IgA or poly-IgA immune complexes showed a dose-dependent effect, consistent with proteinuria reduction during telitacicept treatment1. ConclusionTelitacicept lowered both circulating Gd-IgA1 and IgA-containing immune complexes, while IgA immune complex levels were more consistent with decreased proteinuria.

ВЛИЯНИЕ ЭЛЛАГОТАНИНОВ И ПРОБИОТИЧЕСКОГО ПРЕПАРАТА “ЭКОФЛОР” НА ПОКАЗАТЕЛИ НЕСПЕЦИФИЧЕСКОГО ИММУНИТЕТА СТЕРЛЯДИ

The experimental study of the effect of feed additive based on ellagotanins and immobilized probiotic “Ecoflor” on some parameters of non-specific immunity of sterlet Acipenser ruthenus was carried out. Feed additives based on ellagotannins are one of the numerous preparations of natural origin. They have antibacterial and immunostimulating properties. Biologically active additive “Ecoflor” is a consortium of live antagonistically active strains of bifido- and lactobacilli immobilized on enterosorbent. During the experiment, control and experimental groups were kept in flow-through plastic pools. Sterlets were fed 6 times a day. Drugs were added to the feed according to the instructions. Sampling was carried out before the start of the experiment, on 7, 14, 30, 45 and 60 days. Material for the study was taken from 5 individuals from each group. Peripheral blood was taken from the tail vein. After autopsy, tissue pieces of immunocompetent organs (kidney, spleen and liver) were taken from the fish into dry sterile tubes. Blood coagulation and clot retraction were waited for to obtain serum. The serum was then withdrawn with a syringe with a fine needle and transferred into an Eppendorf tube. For further studies, the homogenate was prepared from kidney, spleen and liver with physiological solution in the ratio of 1:6. The level of bacteriostatic activity, C-reactive protein and nonspecific immune complexes was determined in blood serum samples. In homogenates of kidney, spleen and liver the content of immune complexes was determined. The study established high indices of antimicrobial properties of blood serum in all studied fish and the absence of immunodeficient individuals. Lower indices of bacteriostatic activity in experimental groups and zero percent of immunodeficient individuals indicate insignificant influence of preparations on nonspecific antibacterial defense. The majority of the studied fish had a positive level of C-reactive protein, which indicates the presence of inflammatory processes in the body. The content of nonspecific immune complexes in the liver was several times higher than in other tissues, which may be due to the neutralization of components included in the preparations.

The Level of Poly-IgA Immune Complexes in the Short-Term Efficacy Evaluation and Disease Activity Monitoring of IgA Nephropathy (IgAN)

The Level of Poly-IgA Immune Complexes in the Short-Term Efficacy Evaluation and Disease Activity Monitoring of IgA Nephropathy (IgAN)

Xanthine oxidase levels and immune dysregulation are independently associated with anemia in Plasmodium falciparum malaria

Severe anemia is an important contributor to mortality in children with severe malaria. Anemia in malaria is a multi-factorial complication, since dyserythropoiesis, hemolysis and phagocytic clearance of uninfected red blood cells (RBCs) can contribute to this syndrome. High levels of oxidative stress and immune dysregulation have been proposed to contribute to severe malarial anemia, facilitating the clearance of uninfected RBCs. In a cohort of 552 Ugandan children with severe malaria, we measured the levels of xanthine oxidase (XO), an oxidative enzyme that is elevated in the plasma of malaria patients. The levels of XO in children with severe anemia were significantly higher compared to children with severe malaria not suffering from severe anemia. Levels of XO were inversely associated with RBC hemoglobin (ρ = − 0.25, p < 0.0001), indicating a relation between this enzyme and severe anemia. When compared with the levels of immune complexes and of autoimmune antibodies to phosphatidylserine, factors previously associated with severe anemia in malaria patients, we observed that XO is not associated with them, suggesting that XO is associated with severe anemia through an independent mechanism. XO was associated with prostration, acidosis, jaundice, respiratory distress, and kidney injury, which may reflect a broader relation of this enzyme with severe malaria pathology. Since inhibitors of XO are inexpensive and well-tolerated drugs already approved for use in humans, the validation of XO as a contributor to severe malarial anemia and other malaria complications may open new possibilities for much needed adjunctive therapy in malaria.

Immune complexes in Kosali breed of cattle naturally exposed to arsenic contamination: Attenuation by

The study investigated the effect of bark powder on circulatory immune complexes and total immunoglobulins level in Kosali breed of cattle environmentally exposed to arsenic contamination through water and fodder. Arsenic concentration was significantly (p&amp;#60;0.05) higher in drinking water, fodder and cattle blood samples collected from the arsenic contaminated area as compared to uncontaminated area (control area). Kosali cattle reared in the arsenic contaminated area showed significantly (p&amp;#60;0.05) elevated circulatory immune complexes with decreased level of plasma total immunoglobulins compared to the control area. bark powder was administered orally @ 40 mg/kg. b. w. daily for 30 days to eight Kosali cattle (treatment group) selected from the arsenic contaminated area. Eight Kosali cattle each from arsenic contaminated and uncontaminated area (without any treatment) served as exposure control and healthy control respectively. Kosali cattle of the treatment group exhibited significant (p&amp;#60;0.05) decrease in the level of circulatory immune complexes; and increase in the total immunoglobulin concentration to the level comparable to that of healthy control.

Ecological and physiological analysis of immune reactions of &lt;i&gt;Pelophylax ridibundus&lt;/i&gt; and &lt;i&gt;P. lessonae&lt;/i&gt; (Amphibia: Ranidae) in anthropogeneously transformed territories

The purpose of the work is to assess the state of Anura populations living in an anthropo-genic territory according to a set of indicators of the body’s immune homeostasis. The objects of the study were Pelophylax ridibundus (Pallas, 1771) and P. lessonae (Camerano, 1882), living in the natural populations of reservoirs in Nizhny Novgorod. Priority chemical pollutants of the water bodies were determined by spectrophotometry. Species were identified using a multiplex PCR test system. The identification results were confirmed by sequencing of the mitochondrial ND2 gene and a fragment of the SAI protein. We counted the numbers of erythrocytes and leukocytes, determined the leukocyte profile and the level of immune complexes in all frogs. An excess of the water quality standard for the content of heavy metals was revealed in all the water bodies. Molecular genetic diagnostics showed the presence of both “pure” P. ridibundus and individuals with introgressive mtDNA of the Anatolian form of the lake frog (P. cf. bedriagae) in the sample of lake frogs. In the sample of pond frogs, all studied individuals had only species-specific mt- and nDNA markers of P. lessonae. P. ridibundus differed from P. lessonae by an increased content of erythrocytes, neutrophils, basophils, small immune complexes, and a reduced content of lymphocytes. The revealed changes in the immunohematological parameters of green frogs were caused by the complex henotoxic effect of pollutants in the water bodies. A decrease in the lymphocytes/eosinophils ratio index was shown with an increase in the concentration of nitrites, an increased activity of humoral immune responses in conditions of sulfate pollution of the aquatic environment, an increase in the proportion of myelocytes in the blood of frogs with an increased concentration of manganese and nitrates in water. Under conditions of environmental stress, the blood regulatory systems of frogs reflected a stress-induced reaction, which was more pronounced in the body of lake frogs compared to pond ones.

Levels of Nonspecific Immune Complexes and Oxidative Processes in Tissues of Alien Bivalve Mollusks Inhabiting the Gorky Reservoir

Levels of Nonspecific Immune Complexes and Oxidative Processes in Tissues of Alien Bivalve Mollusks Inhabiting the Gorky Reservoir

Comparative Analysis of FCGR Gene Polymorphism in Pulmonary Sarcoidosis and Tuberculosis.

The clinical outcome of sarcoidosis (SA) is very similar to tuberculosis (TB); however, they are treated differently and should not be confused. In search for their biomarkers, we have previously revealed changes in the phagocytic activity of monocytes in sarcoidosis and tuberculosis. On these monocytes we found a higher expression of receptors for the Fc fragment of immunoglobulin G (FcγR) in SA and TB patients vs. healthy controls. FcγRs are responsible for the binding of immune complexes (ICs) to initiate an (auto)immune response and for ICs clearance. Surprisingly, our SA patients had a high blood level of ICs, despite the abundant presence of FcγRs. It pointed to FcγR disfunction, presumably caused by the polymorphism of their (FCGR) genes. Therefore, we present here an analysis of the occurrence of FCGR2A, FCGR2B, FCGR2C, FCGR3A and FCGR3B variants in Caucasian SA and TB patients, and healthy individuals with the use of polymerase chain reaction (PCR) and real-time PCR. The presented data point to a possibility of supporting the differential diagnosis of SA and TB by analyzing FCGR2C, FCGR3A and FCGR3B polymorphism, while for severe stages of SA also by studying FCGR2A variants. Additionally, the genotyping of FCGR2A and FCGR3B might serve as a marker of SA progression.

Systemic lupus erythematosus with diffuse alveolar hemorrhage.

Systemic lupus erythematosus with diffuse alveolar hemorrhage.

Mycobacterium tuberculosis Cell Wall Antigens Induce the Formation of Immune Complexes and the Development of Vasculitis in an Experimental Murine Model.

Tuberculosis (TB) of the central nervous system (CNS) presents high mortality due to brain damage and inflammation events. The formation and deposition of immune complexes (ICs) in the brain microvasculature during Mycobacterium tuberculosis (Mtb) infection are crucial for its pathobiology. The relevance of ICs to Mtb antigens in the pathogenesis of CNS-TB has been poorly explored. Here, we aimed to establish a murine experimental model of ICs-mediated brain vasculitis induced by cell wall antigens of Mtb. We administered a cell wall extract of the prototype pathogenic Mtb strain H37Rv to male BALB/c mice by subcutaneous and intravenous routes. Serum concentration and deposition of ICs onto blood vessels were determined by polyethylene glycol precipitation, ELISA, and immunofluorescence. Histopathological changes in the brain, lung, spleen, liver, and kidney were evaluated by hematoxylin and eosin staining. Our results evidenced that vasculitis developed in the studied tissues. High serum levels of ICs and vascular deposition were evident in the brain, lung, and kidneys early after the last cell wall antigen administration. Cell wall Mtb antigens induce strong type III hypersensitivity reactions and the development of systemic vasculitis with brain vascular changes and meningitis, supporting a role for ICs in the pathogenesis of TB.

The development of immunosorbents for the treatment of systemic lupus erythematosus via hemoperfusion.

Systemic lupus erythematosus (SLE) is an autoimmune disease (AID) that involves multiple organ systems and is characterized by elevated levels of autoantibodies (ANA) and immune complexes. The immunoadsorption technique uses an extracorporeal clearance process to remove pathogenic toxins from patients' blood and alleviate disease symptoms. An immunosorbent is a key component of the immunoadsorption system that determines therapeutic efficacy and safety. Immunosorbents are prepared by immobilizing antibodies, antigens, or ligands with specific physicochemical affinities on a supporting matrix. Immunosorbents and pathogenic toxins bind via affinity adsorption, which involves electrostatic interactions, hydrogen bonds, hydrophobic interactions, and van der Waals forces. Immunosorbents are classified on the basis of their interaction mechanism with toxins into three categories: non-selective, semi-selective, and highly selective. This review aimed to summarize the current status of various commercial immunosorbents that are used to treat SLE. Moreover, recent developments in immunosorbents have heightened the need for a brief discussion about specific ligands and a supporting matrix.

Immune complex-mediated neutrophil activation in patients with polymyalgia rheumatica.

Neutrophils are important in host defence. However, neutrophils are also linked to inflammation and organ damage. The purpose of this study was to assess whether markers of neutrophil activation are increased in PMR. Levels of immune complexes (IC), calprotectin and neutrophil extracellular traps (NETs) were measured in plasma of healthy individuals (n = 30) and patients with PMR (n = 60), at flare and upon treatment with glucocorticoids using ELISA. Plasma-mediated neutrophil activation was assessed in presence of an FcγRIIA inhibitory antibody (IV.3). Plasma levels of calprotectin and NETs were elevated in PMR (P < 0.001). Mechanistically, neutrophil activation was driven by ICs, present in plasma, able to up-regulate neutrophil activation markers CD66b and CD11b (P < 0.0001) in an FcγRIIA-dependent manner (P < 0.01). Of note, circulating levels of IC correlated with plasma induced CD66b and CD11b (r = 0.51, P = 0.004, and r = 0.46, P = 0.01, respectively) and decreased after glucocorticoid therapy. In contrast to NETs, calprotectin significantly decreased after glucocorticoid therapy (P < 0.001) and was higher in PMR without overlapping GCA compared with patients with overlapping disease (P = 0.014). Interestingly, musculoskeletal involvement was associated with elevated levels of calprotectin before initiation of glucocorticoid therapy (P = 0.036). Neutrophil activation, including NET formation, is increased in PMR, through IC-mediated engagement of FcγRIIA. Clinically, neutrophil activation is associated with musculoskeletal involvement, with calprotectin, but not NETs, being a biomarker of treatment response in PMR patients. In all, IC-mediated neutrophil activation is a central process in PMR pathogenesis identifying potential novel therapeutic targets (FcγRIIA), as well as soluble markers for disease monitoring (calprotectin).

Low Level of Advanced Glycation End Products in Serum of Patients with Allergic Rhinitis and Chronic Epstein-Barr Virus Infection at Different Stages of Virus Persistence.

Advanced glycation end products (AGEs) are formed in a nonenzymatic reaction of the reducing sugars with amino groups of proteins, lipids, and nucleic acids of different tissues and body fluids. A relatively small number of studies have been conducted on the role of AGEs in allergic inflammation. In this study, patients with allergic rhinitis (AR) were examined for the presence of Epstein-Barr virus and the content of fluorescent and nonfluorescent AGEs. We have also determined the level of a unique epitope (AGE10) which was recently identified in human serum using monoclonal antibodies against synthetic melibiose-derived AGE (MAGE). The levels of AGE10 determined with an immunoenzymatic method revealed no significant difference in the patients' blood with intermittent AR and chronic EBV persistence in the active and latent phases. It has been shown that there is a statistically significantly smaller amount of AGEs and pentosidine in groups of patients, both with and without viremia, than in healthy subjects. In turn, higher levels of immune complexes than of AGE10 were detected in the groups of patients, in contrast to the control group, which had lower levels of complexes than AGE10 concentration. In patients with active infection, there is even more complexes than of noncomplexed AGE10 antigen. The lower level of AGE in allergic rhinitis patient sera may also be due, besides complexes, to allergic inflammation continuously activating the cells, which effectively remove glycation products from the body.

Laboratory validation of an ELISA method to measure circulating immune complexes levels in canine visceral leishmaniasis

Susceptible dogs suffering from canine leishmaniasis (CanL) develop an ineffective humoral immune response that leads to the formation of circulating immune complexes (CIC). These CIC are aggregates of Leishmania proteins and anti-Leishmania immunoglobulins. Their deposition in different tissues is considered the main cause of mortality. For this reason, CIC have been suggested as an excellent CanL biomarker for measuring the progression of the disease and the effectiveness of specific treatments. The present study aims to perform a laboratory validation of a Leishmania-specific method to isolate and quantify CIC in dog serum samples. CIC isolated from serum samples of infected dogs, grouped according to the LeishVet classification, were quantified following a PEG-ELISA procedure. The validation established a cut-off of 0.274 OD. All the parameters analyzed (including linearity, specificity, precision, and robustness) fulfilled the defined criteria, confirmed by statistical analyses. The results also proved the reproducibility and reliability of the method when samples were tested under the same conditions, and the consistency and usefulness of the method for an optimal staging of infected dogs. In conclusion, the laboratory validated method offers a potent tool to clinicians for a proper CanL management and to measure the progression of the disease.

Benzo(A)Pyrene-Induced Lung Cancer: Chemo Protective Effect of Coronarin D in Swiss Albino Mice.

Lung cancer is considered one of the most prevalent cancers worldwide and also has a high death rate. The prevalence of lung cancer is high in developed countries than in developing countries due to the lifestyle changes and quality of air. Coronarin D is a diterpene, which is isolated from the Hedychium coronarium. It demonstrated several pharmacological properties such as anti-allergic, anti-inflammatory, antimicrobial, and anticancer activities. In the current investigation, the potential of Coronarin D on the B(a)P-induced lung cancer was studied in the experimental mice model. The B(a)P-administrated animals exhibited a reduced level of immune cells, IgG, IgM, immune complexes, SOD, and CAT. The B(a)P-administrated animals expressed high levels of IgA, LPO, xenobiotic markers, tissue marker, tumor marker, and proinflammatory cytokines. On treatment with Coronarin D, the level of neutrophils, lymphocytes, leucocytes, and absolute neutrophils was elevated in the B(a)P-administered mice. The immune complex was augmented in the Coronarin D-treated animals in comparison with B(a)P-treated mice. The level of IgG and IgM was increased, whereas the level of IgA was reduced in the Coronarin D-treated animals. The level of LPO was downregulated, whereas the level of SOD and CAT was upregulated in Coronarin D-treated animals. The expression level of xenobiotic markers, tissue marker, tumor marker, and proinflammatory cytokines was reduced in the Coronarin D-treated animals. The histopathological results revealed that lung tissues of Coronarin D-treated animals had less alveolar damage with decreased hyperplasia. These findings suggest that the Coronarin D can be utilized as a potent chemopreventive agent for treating lung cancer in the future.