An analytical approach has been considered to inspect the flow and heat transfer of immiscible Newtonian and micropolar fluid in a porous medium. This study aims to elucidate the Darcy effect on the model in which isotropic porous regions with different permeability are used. The model of the current problem explains that the flow region is divided into two regions: Newtonian fluid flows in the upper layer, and micropolar fluid flows in the lower layer. Different constant temperatures imposed at boundary walls, heat transfer does not affect the pressure gradient. The governing equations are solved analytically by applying a linear differential equation (LDE). The effect of associated physical parameters such as material parameter, Darcy number, Eckert number, Prandtl number, and viscosity ratio on velocity, micro-rotation, heat transfer, flow rate, heat transfer rate, wall shear stress, and Nusselt number have been inspected. The most significant finding of this research work is that increasing the Darcy number signifies enhanced permeability, resulting in a higher flow rate. The heat transfer rate at the top occurs maximum when the material parameter’s range is minimum while raising the viscosity ratio leads to an increasing heat transfer rate at the bottom. Enhancement in material parameter influences Nusselt number and decreases in nature. The findings of our study are verified with the previously established results. The present work has a setup that is useful in petroleum extraction, transport problems in reservoir rock of an oil field, improving nutrient transport and thermal regulation in tissue engineering, and designing more efficient drug delivery systems and biomedical devices.