Immersion Of Seeds Research Articles

PHYSIOLOGICAL ACTIVITY ANALYSIS AND GROWTH OF EBONY SEEDLINGS (Diospyros celebica Bakh) TREATED BY VARIOUS BIOFERTILIZER COMBINATIONS

Ebony is one of slow-growing spe- cies), that of suspected to be the triggering factors to prone to extinction. In addition, the nature of ebony seeds is recalcitrant, so they cannot be stored for a long time. Other factors affecting seed germination are the degree of maturity, size, and weight of the seeds. Seeds harvested before the physiological maturity level would not have high viability and even could not germinate well due to the in-sufficient of food reserves, and un-perfect embryo formatio. Bio-fertilizer is used to help accelerate the growth of ebony. This study aims to analyze the combination of bio fertilizers which would be giving a better effect on the physiological activity and growth of ebony seedlings. Morphological and physiological variables measurement were used in this study. The research design used a Completely Randomized Design at a real level of 5%. If the fingerprint results obtained have a real effect, then a further test of the Duncan Multiple Range Test is carried out. Re- search results showed that seed coating treatment of 45% Penicillium microbial solution + seed coating) gave the best results in each variable of both growth and physiology of ebony seedlings, while in the treatment without seed coating, Sargassum microbial solution 30% indicates good results in each variable of growth and physiology of ebony seedlings. Treatment with the immersion of seeds in microbes can produce a high percentage in each variable.

How to access viability of Zanthoxylum rhoifolium seeds? A protocol of tetrazolium test as an alternative to evaluate a dormant seed

A tetrazolium test method is proposed for evaluating seed quality of Zanthoxylum rhoifolium Lam. We used seeds from two lots, which we immersed in distilled water for 8 hours and subjected to two preparations: A - lateral incision into the seed, parallel to the hilum, to expose the endosperm, and B - extraction of the embryos. We then exposed the seeds of both preparations to the tetrazolium solution at two concentrations (0.1% and 0.05%) for two periods (24 hours and 48 hours) at a temperature of 25 ºC, then the seeds were evaluated in a 2 × 8 factorial scheme (lots × treatments) and classified in viable and non-viable. We found that the best preparation for the tetrazolium assay in Zanthoxylum rhoifolium was to remove part of the seed coat, this allowed the solution to penetrate the internal tissues without damaging the embryo. There were no significant differences between concentrations of 0.1% at 24 hours and 0.05% for 48 hours. We concluded that immersion of seeds in water for 8 hours, followed by a lateral cut parallel to the hilum exposing the endosperm at a concentration of 0.1% tetrazolium for 24 hours or 0.05% for 48 hours is an efficient method for quality assessment of Zanthoxylum rhoifolium seeds.

The effects of sodium metabisulfite (Na2S2O5) on the physicochemical properties of breadnut seeds (artocarpus camansi)

Starch can be produced from many plant varieties and through various processing methods. Breadnut (Artocarpus camansi) is a plant variety containing seeds that are rich in carbohydrates to produce starch. However, it has not been widely used and converted into useful products because it is still traditionally considered waste. Starch can be produced physically, chemically, and enzymatically. In this study, the starch extraction from breadnut seeds was conducted using a chemical method, namely immersion into sodium metabisulfite (Na2S2O5) at concentrations of 600, 800, and 1000 ppm. Characterization includes starch content, water content, amylose content, crystallinity using XRD (X-Ray Diffraction), functional groups using FTIR (Fourier Transform Infrared Spectroscopy), and morphology using SEM (Scanning Electron Microscope). The results showed the immersion of breadnut seeds into sodium metabisulfite affected the physicochemical properties. The produced starch content ranged from 53.56-54.87%; water content ranged from 5.83-6.66%; amylose content ranged from 27.07-30.80%. Based on the X-ray diffraction pattern, breadnut seed starch belonged to Type B which reached the highest peak at 17° 2θ. The functional group also showed the same FTIR spectrum in the breadnut seeds starch. Meanwhile, the morphological result showed that the starch granules of breadnut seeds ranged from 6.6 μm to 8.1 μm.

Effect Trichoderma spp on Wheat ( Triticum aestivum)

The objective this study were to determine effect of Trichoderma spp and Humic acid 60% on three Varieties of wheat (Triticum aestivum). The study included two factors, first factor was immersion of seeds in Humic acid 60% solution Humic acid 60% + Trichoderma spp150Mg/l, Humic acid 60% + Trichoderma spp 250Mg/l, symbols T1, T2, T3, T4 respectively. The second factor is three varieties of wheat (Iba99, Adina, Dijlla) and the plant variety is represented by V1, V2, V3 respectively. Treatments were Design (RCBD) with three replicates Statistics were evaluated followed by Duncan multiple range test to compare means at P< (0.05). Data presented the use of Humic acid 60% + Trichoderma spp250 Mg/l led to a significant increase in the studied traits as well as the interaction between variables where treatment V2T4 were highest significant increase in plant height, root length and spike length, which reached 95.07 cm plant height, 14.93 cm length Root, spike length is 11.50 cm, while treatment V1T4 were the highest significant increase in leaf reach, reaching 26.80 cm.

Effectiveness of Long Immersion of Rice Seeds in Local Microorganism Culture (MOL) of Banana Weevils on Rice Growth and Production

The need for rice from year to year continues to increase due to the growing population of Indonesia, but this is not balanced with sufficient rice production. This is what makes it difficult for Indonesia to be self-sufficient in food so that efforts are needed to increase rice production nationally so that domestic rice needs are met. This panel aims to see the effectiveness of long soaking of rice seeds in MOL banana weevil on vegetative growth and yield of Ciherang variety rice plants. The research was conducted in November 2021 – March 2022 on open land of BPP Caringin, Legok District, Tangerang Regency. The study was conducted using the Randomized Complete Group Design (RKLT) method consisting of 5 treatments, namely control (without soaking MOL banana weevil), soaking rice seeds for 6 hours, 12 hours, 18 hours, and soaking for 24 hours. Each treatment was repeated 5 times so that there were 25 experimental units. Each experimental unit consists of three plants, the number of plants to be studied is 75 plants. Soaking seeds in MOL banana weevil for 24 hours gives the best results for the growth and production of Ciherang rice plants.

Gibberellic Acid Concentrations and Storage of Caryocar brasiliense (Caryocaraceae) Seeds Propagated in Tubes

The immersion of seeds in gibberellic acid solutions can promote dormancy breaking. Storage can be dynamic in seed longevity. The objective of this work was to evaluate different concentrations of gibberellic acid applied to seeds isolated from two storage times on the emergence of C. brasiliense tree seedlings grown in tubes. There were two experiments with different planting and storage times. The seeds were extracted from the endocarp. In both, a randomized block design was used with the following seven treatments: 0, 50, 100, 500, 1000 and 2000 mg L−1 of GA3 and dry seeds without pre-soaking, with five replications of 20 seeds. The seeds were treated with fungicide and sown in tubes containing substrate. After 60 days, evaluations and transplantation were carried out. In the experiment with older pyrenes (seeds stored for seven months), there was high seed mortality (96.54%) and a small emergence rate (3.45%). On the other hand, when new pyrenes were used, the percentage of dead seeds was 32.71% and emergence was 62.28%. GA3 did not stimulate germination or the diameter of C. brasiliense tree seedlings, except for stem length. The 2000 concentration provided a greater stem length and greater leaf area compared to dry seeds.

Improvement of In Vitro Seed Germination and Micropropagation of Amomum tsao-ko (Zingiberaceae Lindl.)

Black cardamom (Amomum tsao-ko Crevost &amp; Lemarié) is a spice plant of great commercial value in Vietnam, but with limited propagation ability. Its seeds are characterized by a thick and hard seed coat, a small endosperm, and a small embryo, which are the causes of the physical dormancy of the seeds and low germination. Attempts in this study to improve the germination rate and achieve uniform germination included mechanical scarification, immersion in hot or cold water, acid scarification, and the application of plant growth regulators. Although immersion of seeds in cold water and application of plant growth regulators (PGRs) (gibberellic acid (GA3) and 1-naphtaleneacetic acid (NAA)) showed positive effects on seed germination and subsequent seedling growth, mechanical scarification provided the highest germination rate of black cardamom seeds (68.0%) and significantly shortened germination time (53.7 days) compared to control (16.0% and 74.7 days). On the other hand, an efficient micropropagation protocol has been established using shoot tip explants derived from in-vitro-grown seedlings. Murashige and Skoog (MS) medium supplemented with 4.0 mg/L 6-benzylaminopurine (BAP) + 0.5 mg/L NAA proved to be most suitable for rapid multiplication and rooting, providing a mean of 5.4 shoots per explant, 6.8 cm shoot length, and 16.2 roots per explant after 7 weeks of culture. Well-rooted black cardamom plantlets have been successfully adapted to ex vitro conditions. “Fasco” bio-soil was more suitable for acclimatization, with a 48.9% survival rate, 23.3 cm plant length, and 5.7 leaves per plant after 3 months of planting. Improved germination and multiplication protocols can be used to improve propagation performances and to develop elite of black cardamom planting material.

Superação de dormência de sementes de Myrciaria glomerata O. Berg

Abstract Myrciaria glomerata O. Berg. (Myrtaceae) is a fruit tree native to the Brazilian Atlantic Forest with potential for use as food and medicine, besides having good ecological potential. However, this species is limited by low germination rates. Thus, this study aimed to evaluate different methods to overcome dormancy in seeds of M. glomerata and to improve seedling vigor. For this, two experiments were conducted. In Experiment 1, seven treatments were tested: a control treatment, immersion in water at 100 ºC for 30 seconds, immersion in concentrated sulfuric acid (H2SO4) for 1 and 5 minutes, immersion in an aqueous solution of gibberellic acid (GA3) at the concentrations of 250 and 500 mg L-1 for 24 h, and seeds homogenized with an aqueous solution of Stimulate® at a concentration of 5 mL kg-1 seed. In Experiment 2, six doses of Stimulate® were tested: 0, 5, 10, 15, 20, 25, and 30 mL kg-1 seed. The immersion of seeds in 250 and 500 mg L-1 GA3 contributes to increase germination rates, seed length, fresh mass, and dry mass compared to the control and the other methods tested. Immersions in water at 100 ºC and in H2SO4 are not recommended for M. glomerata. The treatment with Stimulate® at 15–25 mL kg-1 seed increased all characteristics analyzed. Immersion in 250 mg L-1 GA3 and Stimu-late® at 20–25 mL kg-1 seed efficiently stimulated seeds to overcome dormancy in M. glomerata, as well as improved seedling vigor.

Morphological Characterization and Physical Dormancy of Bauhinia winitii Seed: Living Collection of Purwodadi Botanic Garden

Bauhinia winitii Craib (Fabaceae, Caesalpinioideae) is a woody climber which is currently included in endangered species list. B. winitii seeds are orthodox seeds in which it has hard coat morphologically. Therefore, B. winitii seeds often undergo physical dormancy which can be broken through immersion in certain liquid media. This study aimed to characterize the morphology of B. winitii seeds and determine the pre-sowing treatment method to accelerate the seeds germination of B. winitii. External morphological characterization was carried out by observing the seeds quantitative and qualitative parameters. Characterization of seeds internal morphology was conducted using a digital microscope. Physical dormancy breaking was carried out by immersion in warm and cold water for 24 hours. Morphological characterization data were analyzed descriptively, while seed germination data were analyzed quantitatively by using one-way ANOVA followed by LSD test (with confidence level of 95%). B. winitii has pod-shaped fruits with 2-6 seeds per pod, the seeds are 1.36 cm x 1 cm in size, 0.47 cm thick and weighed 0.5 g, oblong to conical in shape, smooth and shine surface, with light to dark brown in color. Seeds immersion treatment in warm water could break the physical dormancy of B. winitii seeds thus 63% of the seeds were able to germinate and it was significantly different compared to control and cold water immersion treatment. Technical to germinate B. winitii seed can be known from pre-sowing treatment. This study can be used as a reference for seed identification and germination technical of B. winitii seed.

Inoculation and colonization of isolated entomopathogenic fungi Beauveria bassiana in rice plants, Oryza sativa L. through seed immersion method

BackgroundThe aims of the present study were to isolate and identify native entomopathogenic fungi, Beauveria bassiana from the Egyptian soil and to evaluate the artificial establishment of B. bassiana as endophytes in rice plants through seeds immersion.ResultsTen soil samples were collected from different cultivated fields at the Ismailia Governorate. Only one sample was positive with a ratio of 10%. Sequencing of the internal transcribed spacer (ITS) region indicated that the isolate obtained from the soil sample belongs to B. bassiana and was registered under the accession no. MN337282. To test the endophytic colonization of B. bassiana, rice seeds were soaked by B. bassiana with a concentration of 5 × 107 spores/ml, to test when B. bassiana become an endophyte in rice plants. The plants were examined for endophytic presence of B. bassiana, 30 days post treatment. PCR amplification using fungal specific primers for a conserved region of β-tubulin gene yielded identical 360 bp products from both B. bassiana and rice treated plants.ConclusionThe results showed that seeds immersion with a conidial suspension proved to be a good method to introduce B. bassiana into rice leaves to protect the rice plants against stem borers.

Influence of scarification and gibberellic acid on seed dormancy and germination of pomegranate seedlings

Contextualization: Due to the importance of pomegranate among fruit species, studies on the physiology of its seeds and the production of seedlings are essential for the expansion of commercial orchards.&#x0D; Knowledge gap: With the portrayed importance of this crop and its use in various sectors, such as agro-industrial and pharmaceutical products, the development of continuous and straightforward propagation techniques that provide a uniform seedling development will provide favorable conditions for the cultivation and exploitation of the species, improving its use and boosting new research. &#x0D; Purpose: To evaluate the effect of scarification and immersion in gibberellic acid on the dormancy of pomegranate seeds, aiming at accelerating the emergence and improving the initial growth of seedlings.&#x0D; Methodology: The sarcotesta was removed and the seeds were scarified. The treatments consisted of a scarified control, a non-scarified control (seeds with and without scarification), and seeds subjected to the concentrations of 500 mg L-1, 1,000 mg L-1, and 1,500 mg L-1 of GA3. The following variables were evaluated: initial emergence, emergence percentage, mean emergence time, leaf number, stem diameter, shoot length, root length, and total dry matter.&#x0D; Results and conclusions: The pomegranate cv. Valencian seeds do not have tegument-related dormancy. In order to favor the emergence percentage and the initial growth of pomegranate seedlings, mechanical scarification and immersion of the seeds in GA3 are dispensed. Scarification and immersion of pomegranate seeds in 500 mg L-1 of GA3 are recommended to produce seedlings in a shorter time.

Propagation of threatened climber species Entada rheedii Spreng. – a medicinal plant with extremely thick and hard seed coat

The study explored propagation techniques of Entada rheedii Spreng., a threatened medicinal climber species with extremely hard seed coat. Propagation trials included both pre-sowing treatments of seeds for germination and clonal propagation by stem cutting. Pre-sowing treatments included (a) soaking of both cut (notched) and uncut (intact) seeds in water for 0 h, 24 h, 48 h, and 72 h and (b) immersion of intact seeds in 5% acetone solution for 5 min, 10 min, and 20 min before sowing in germination media in polybags. On the other hand, stem cutting involved treating the summer or autumn cuttings with 0%, 0.4%, and 0.8% IBA solution before rooting in non-mist propagator. Notched seeds soaked in water for 48 h showed the fastest seed germination with the highest germination percentages (73.3) and better seedling growth in terms of plant height, collar diameter, leaf number and total dry mass followed by notched seeds soaked in water for 72 h. The slowest germination and the lowest germination percentage (3.3), as well as the poorest growth performance was for intact seeds without any treatment. The highest rooting percent­age with maximum number of roots (36.6) was obtained from the summer cuttings treated with 0.4% IBA solution followed by autumn cuttings with 0.8% IBA and the lowest (43.3% and 8.3 roots) was for summer cuttings in control. The factors also dictated the survival and growth performance of rooted cuttings in the nursery conditions. The outcomes of these trials i.e., notched seeds soaking in water for 48 h will help to enhance the propagation of this valuable medicinal plant species.

Potassium nitrate to overcome dormancy of Urochloa humidicola Comum seeds

The main difficulty with propagating Urochloa humidicola Comum is the high percentage of seed dormancy. Thus, the objective of this study was to develop a treatment to overcome seed dormancy and promote seed germination of this species that can be applied at a large scale. This study was conducted in two stages. First, the following treatments were evaluated using one seed lot: control (without treatment), immersion in H2SO4 (98%, 36N) for 10 min, moistening the germination substrate with KNO3 (0.2%) and immersion in KNO3 at concentrations of 0%, 2%, 4%, 6%, and 8% for periods of 12, 24, 36, and 48 h, followed by drying in the shade. In the second stage, three seed lots were evaluated in all of the aforementioned treatments, except for those of immersion in 0% KNO3 and of all concentrations for 36 and 48 h. The seeds were evaluated for water content, germination, percentage of abnormal seedlings, dead and dormant seeds, first count, and average germination time. Immersion in the KNO3 solution was efficient in overcoming dormancy and promoting germination. To be used by companies, the immersion of seeds in a 4% KNO3 solution for 24 h was the most suitable treatment to overcome dormancy and promote the germination of U. humidicola Comum seeds.

Germinación de semillas de Quercus humboldtii Bonpl. (Fagaceae): especie vulnerable del bosque Altoandino

El roble colombiano (Quercus humboldtii) es una especie endémica, dominante y vulnerable de los bosques altoandinos. Su madera y fruto se han utilizado tradicionalmente en pequeña escala por las poblaciones rurales. Una mayor sincronía del proceso de germinación podría facilitar la producción de plántulas para su uso en los programas de conservación. En vivero, se evaluó la emergencia mediante la inmersión de semillas en cuatro soluciones de ácido giberélico (GA3) por 24 horas (0, 150, 300 y 600 mg L-1), a 4 °C. Se adoptó un diseño completamente al azar con cuatro repeticiones de 25 semillas. El tiempo medio de emergencia tuvo una media de 32 días; sin diferencias estadísticamente significativas entre las diferentes dosis. No hubo efecto del GA3 para la emergencia de plántulas hasta 300 mg L-1 (media= 92 %); sin embargo, fue desfavorable con 600 mg L-1 (20 %). Las semillas frescas de roble andino poseen un alto contenido de humedad (38 %). Lo anterior revela un probable comportamiento recalcitrante. Este estudio confirma, como en la mayoría de los encinos blancos del trópico, la ausencia de latencia y la alta viabilidad de sus semillas. El uso del GA3 no es un tratamiento pregerminativo útil para acelerar y uniformizar la emergencia de plántulas de Q. humboldtii.

Влияние криоконсервации на выживаемость семян Chamomilla reticuta сорта «Подмосковная» и химический состав эфирного масла

The article examines the effect above critical low temperatures on the quantitative and qualitative composition of essential oil of Chamomilla reticuta varia «Podmoskovnaya». Seeds of the studied plant species were treated with ultra-low temperature (–196 ºC) before planting in open ground and the viability of the seeds was determined under laboratory conditions. Cryo processing was carried out by direct immersion of seeds in plastic tubes in Dewar vessels with liquid nitrogen. Cryoprotectants were not used. Cryo processing duration was 1 hour, 3 hour and step freezing. Step freezing of seeds was performed in the following sequence: 1 hour in the refrigerating compartment (+ 4 ºC), 1 hour in the freezing compartment (–8 ºC) and 1 hour in liquid nitrogen. In all cases of the experiment, a slow thawing regime was applied. After cryogenic exposure, the material was planted into open ground. Recovery of essential oil was carried out by hydro distillation on a Clevenger apparatus. The component composition of the essential oils was determined on a Clarus-SQ 8 gas chromatograph with a mass spectrometric detector. As a result of the study, it was found that the effect of ultra-low temperatures on the seed material of the Moscow Region chamomile did not negatively affect the quantitative and qualitative composition of the essential oil of the pharmacy chamomile. On the contrary, this method of treating seeds has had a positive effect on the quantitative composition of the essential oil during the growth of the plant and in some cases has even increased the percentage of certain components, such as αfarnesene, β-farnesene, spatulenol, cis-en-in-dicycloether.

Germination and growth of the tree Handroanthus chrysanthus (Bignoniaceae) under nursery conditions

Introduction: Selective logging of forests has significantly diminished the densities of Handroanthus chrysantha Jacq. in Colombia. Therefore, there is a need for studies that contribute to its management. Objective: We aimed to evaluate seed germination and the growth of seedlings under nursery conditions. Methods: Four pre-germinative, four organic substrates and two luminosity conditions were tested. We used a random multifactorial experimental design composed of 32 controlled factors. The main components were immersion of seeds in water at room temperature at different times, combinations of organic substrates and exposure of the factors to shade and sun. Results: We recorded 395 and 290 germinated seeds, corresponding to 51 and 38% of the total sown under shade and sun, respectively. The factors with the best germination values were immersion for 24 hours with alluvial sand and immersion for 24 hours with river sand of mixed with ant soil (2:1) under shade and, without immersion with alluvial sand+ground of ant 2:1 and immersion for 24 hours with chicken manure+ant soil 2:1 under sun. The highest growth in height, vigour and survival was observed in seedlings exposed to full sunlight in any of the combinations of organic substrates. Conclusion: The propagation of H. chrysantha should germinate the seeds under shadow and then expose them to the sun to stimulate their growth..

Superação de dormência de sementes e desenvolvimento de mudas de jatobazeiro em substratos alternativos

The propagation of the jatoba (Hymenaea courbarilL.) by seeds is highly promising in the recovery of degraded areas. Thus, the present research aimed to compare methods forjatoba plant seeds dormancy overcomingand to evaluate the development of seedlings of this species in alternative substrates. In the first experiment, in screened nursery, a completely randomized design was used, with four replications and seven seed treatments: scarification with sandpaper(SS), SS and immersion in water for 24 h, SS and immersion in water for 48 h, intact seed, seed immersed in water for 48 h and seed immersed in water for 96 h. The seedlings that emergency were counted every other days, from 13 to 51days after sowing, quantifying the emergence percentage, the emergence speed index and the emergence average time.The second experiment, in greenhouse,was composed ofthree replicates, in randomized blocks, being seven substrates were used:commercial substrate (CS); 40% SC + 60% forest humus (FH); 40% SC + 60% charcoal rice husk (CRH); 40% SC + 60% bovine manure (BM); 40% CRH + 60% BM; 40% CRH + 60% FH; e 40% FH + 60% BM. At 50, 70 and 120 days after sowing were evaluated the parameters: seedlingheight, leafsnumber, stem diameter, fresh matterand dry matter of aerial partand roots, and leaf area.At 120 DAS, the chlorophyll index was also evaluated. The scarification with sandpaper and immersion of seeds in water for 24 hours presented a greater emergence (96.4% and IVE = 0.697).The mixture containing 40% FHand 60% BMresulted seedlings with a larger leaf area, chlorophyll index and roots and aerial part dry matter, at 120 days after sowing.

Seed vigor of Piptadenia moniliformis Benth. subjected to thermal stress

The objective of this work is to evaluate the influence of accelerated aging test at 41ºC on the germination and seed vigor of Piptadenia moniliformis Benth. Two experiments were conducted. Experiment 1: seeds were submitted to dormancy overcoming treatments. They were immersed into sulfuric acid for 0 (intact seeds), 5, 10, 15, 20 and 25 minutes. Experiment 2: two methods were evaluated for the accelerated aging test at 41ºC, that is, the traditional method and the saturated NaCl solution method with exposure periods of 0, 24, 48, 72 and 96 hours. After each treatment, the seeds were subjected to electrical conductivity and germination tests. Seeds overcame their dormancy when immersed into concentrated sulfuric acid for 5, 10, 15, 20 or 25 minutes, but the germination was faster when immersed for 20 minutes. The different methods for the accelerated aging test, decreased the germination potential and seed vigor of P. moniliformis after 24 hours of exposure. Regarding the method used, the saturated solution provided, in general, the best results since the seed water content was lower, thus reducing the proliferation of fungi. The electrical conductivity test showed that, by increasing the aging period regardless of the method, there is an increase in seed deterioration, corroborating with germination results, which showed that the correlation was moderate and negative and indicated that the higher the conductivity, the lower the percentage and the germination speed index. The immersion of seeds of P. moniliformis into concentrated sulfuric acid for 20 minutes provides a fast and increased germination. The methods for accelerated aging at 41ºC, traditional and saturated solution, can be used as vigor tests to evaluate the physiological quality of seeds.

Effectivity of Trichoderma harzianum as Bio-fungicide Against Moler Disease and Bio-stimulator of Shallot Growth

Moler disease is the primary disease that disturbs the shallot’ cultivation. Hence, it is beneficial to have preventive measures to mitigate the risk and to improve growth. The research objective is to know the effectivity of Trichoderma harzianum suspension as a bio-fungicide and bio-stimulator. The study used seven treatments, i.e., immersion of shallot seeds on negative control (distilled water), positive control (synthetic fungicide+ZPT 1g/liter), T.harzianum suspension with different concentrations of 2 ppm, 4 ppm, 6 ppm, 8 ppm, and 10 ppm, assigned in a Completely Randomized Design (CRD). Treatment that results in a significant level will be continued to the Least Significance Different (LSD) 5% test. The observed variables are the intensity of moler disease and the growth of shallot seeds. Experiment results show the significant difference between treatments. The optimal treatment to reduce the percentage of moler disease intensity is the immersion of shallot seeds in 10 ppm-concentrated T.harzianum suspension, while to improve the shallot growth is in 8 ppm-concentrated T.harzianum suspension. The intensity of moler disease has a negative correlation to the plant’s height and leaf’s diameter but shows no correlation pattern to the number of leaves, wet weight, dry weight, and length of roots.

Germination and Seedling Growth Response on Lithocarpus elegans (Fagaceae) Seeds to Pre-sowing Treatments and Fertilizer Application

This study was conducted to investigate the effect of different pre-sowing treatments of seeds on germination and growth performance of native threatened tree species Lithocarpus elegans at the nursery of Chittagong University, Bangladesh. Seeds were placed to eight pre-sowing treatments e.g. control (PT0), treated with sand paper rubbing (PT1), nicking (PT2), seeds immersed in cold water for 24 hours (PT3), seeds immersed in cold water for 48 hours (PT4) and seeds immersed in cold water for 7 days (PT5), seeds sown at propagator house (PT6), seeds immersed in cold water for 7 days and then sown at propagator house (PT7). Germination percentage was found highest (100%) in seeds sown at propagator house (PT6) followed by seeds immersion in cold water for 24 hours (PT3) (91.11%). For fertilizer dose experiment to seedlings at the nursery level, treatment FT1: 100 kg/ha (0.33679 g urea/pot/seedling) comparing with other treatments FT0: 0 kg/ha (Control), FT2: 200 kg/ha (0.67358 g urea/pot/seedling) showed better performance in case of shoot length, collar diameter, number of leaves, shoot fresh weight, total fresh weight, total dry weight, volume index. Finally, it can be concluded that Lithocarpus elegans seedlings revealed better performances under the seed pre-treatment PT6 (seeds sown at propagator house) and fertilizer dose FT1 (100 kg N ha-1) in growth and biomass production.