Imine Linkage Research Articles

1-(Pyrazin-2-yl)ethylidene]hydrazine: a new multitopic ligand for the design of hybrid molecular frameworks.

Hydrazones and their derivatives are closely related to imine compounds and are potential antimicrobial agents. They have also found application in supramolecular chemistry as multitopic ligands to link multiple metal centres for the design of hybrid molecular frameworks. The molecule of the title compound, C6H8N4, consists of an imine linkage with an N-N bond length of 1.3540 (14) Å. This asymmetric compound is nearly planar and adopts an E configuration about the azomethine C=N double bond. In the solid state, there are two intermolecular N-H...N interactions that interconnect the molecules into a two-dimensional network. The three-dimensional arrangement of the crystal packing is further stabilized by intermolecular π-π interactions interconnecting the centroids of the heterocyclic rings.

Design of pH-sensitive methotrexate prodrug-targeted curcumin nanoparticles for efficient dual-drug delivery and combination cancer therapy.

AimWe designed acid-labile methotrexate (MTX) targeting prodrug self-assembling nanoparticles loaded with curcumin (CUR) drug for simultaneous delivery of multi-chemotherapeutic drugs and combination cancer therapy.MethodsA dual-acting MTX, acting as both an anticancer drug and as a tumor-targeting ligand, was coupled to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[aldehyde(polyethylene glycol)-2000] via Schiff’s base reaction. The synthesized prodrug conjugate (DSPE-PEG-Imine-MTX) could be self-assembled into micellar nanoparticles (MTX-Imine-M) in aqueous solution, which encapsulated CUR into their core by hydrophobic interactions (MTX-Imine-M-CUR).ResultsThe prepared MTX-Imine-M-CUR nanoparticles were composed of an inner hydrophobic DSPE/CUR core and an outside hydrophilic bishydroxyl poly (ethyleneglycol) (PEG) shell with a self-targeting MTX prodrug corona. The imine linker between 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[aldehyde(polyethyleneglycol)-2000] and MTX, as a dynamic covalent bond, was strong enough to remain intact in physiological pH, even though it is rapidly cleaved in acidic pH. The MTX-Imine-M-CUR could codeliver MTX and CUR selectively and efficiently into the cancer cells via folate receptor-mediated endocytosis followed by the rapid intracellular release of CUR and the active form of MTX via the acidity of endosomes/lysosomes. Moreover, the MTX-Imine-M-CUR resulted in significantly higher in vitro and in vivo anticancer activity than pH-insensitive DSPE-PEGAmide-MTX assembling nanoparticles loaded with CUR (MTX-Amide-M-CUR), MTX unconjugated DSPE-PEG assembling micellar nanoparticles loaded with CUR (M-CUR), combination of both free drugs, and individual free drugs.ConclusionThe smart system provided a simple, yet feasible, drug delivery strategy for targeted combination chemotherapy.

Design and synthesis of imine linked ZnO nanoparticles functionalized with Al(III), candidate for application in light emitting diodes

Zinc oxide nanoparticles 1.ZnO decorated with dipodal organic receptor 1 bearing imine linkage were synthesized using wet chemical precipitation technique. The imine linked receptor 1 used as capping agent was synthesized using condensation reaction. 1.ZnO nanoparticles were characterized through FTIR, SEM, TEM, DLS and fluorescence spectroscopy. The emission spectra of surface modified ZnO nanoparticles authenticated the passivation of surface defects and dangling bonds which otherwise are available on the surface of uncapped ZnO nanoparticles. 1.ZnO nanoparticles demonstrated blue emission at 461 nm. The binding affinity of 1.ZnO nanoparticles was also checked in presence of metal ions through changes observed in the emission spectra. An enhancement up to threefold in the fluorescence intensity was achieved from 1.ZnO nanoparticles upon coordination with Al3+ metal ion. The results demonstrated that our design strategy resulted in pure and narrow emission with FWHM 70 nm. This effective way of achieving the selectivity factor and enhanced quantum yield can be utilized in electronic device like light emitting diodes.

PH-sensitive doxorubicin-conjugated prodrug micelles with charge-conversion for cancer therapy

Intelligent drug delivery systems with prolonged circulation time, reduced drug leakage in blood, target site-triggered drug release and endosomal escape are attractive and ideal for malignant tumor therapy. Herein, doxorubicin (DOX)-conjugated smart polymeric micelles based on 4-carboxy benzaldehyde-grafted poly (L-lysine)-block-poly (methacryloyloxyethyl phosphorylcholine) (PLL(CB/DOX)-b-PMPC) copolymer are prepared. DOX and electronegative 4-carboxy benzaldehyde are conjugated to the PLL block via an imine linkage and as a result, the drug loaded micelles exhibited the pH-triggered charge-conversion property and accelerated drug release at tumor pH. In vitro cytotoxicity studies of these DOX-loaded micelles exhibited great tumor inhibition against HeLa and 4T1 cells. Moreover, in mice models of breast cancer, these DOX-loaded micelles showed better anti-tumor efficacy and less organ toxicity than free drug. In summary, these polymeric micelles could be applied as potential nanocarriers for cancer therapy. Statement of SignificanceAs a typical anti-cancer drug, Doxorubicin (DOX) exhibited remarkable tumor inhibition but was limited by its low drug utilization and strong toxicity to organs. To overcome these challenges, we developed a DOX-conjugated polymeric micelle as a nano drug carrier which was endowed with pH-sensitivity and charge-conversion function. The structure of micelles would quickly disintegrate with surface charge-conversion in acidic environment, which would contribute to the endosomal escape and accelerated drug release. These DOX-conjugated micelles would provide a promising platform for the efficient DOX delivery and better anti-cancer efficiency.

New Water-Soluble Polymeric Prodrugs of 2-n-propylquinoline: Synthesis and Evaluation of In Vitro and In Vivo Activities Against Leishmania donovani

2-n-propylquinoline (2-PQ) is a natural compound having interesting anti-leishmanial activities by the oral route but with a rapid elimination rate. 2-PQ is insoluble in water and therefore cannot be administered intravenously. In this study, water-soluble polymeric prodrugs of 2-PQ were synthesized for intravenous administration with the objective of treating patients having severe visceral leishmaniasis with recurrent vomiting. As 2-PQ has no functional groups for tethering onto a soluble polymer, two amine derivatives of 2-PQ, (E)-2-(3-(2-ammonioethoxy)-3-oxoprop-1-en-1-yl)quinolin-1-ium chloride (1) and (E)-2-(3-(2-ammonioethyl)amino)-3-oxoprop-1-en-1-yl)quinolin-1-ium chloride (2), were synthesized and characterized using 1H and 13C NMR, IR, and mass spectroscopy. Both compounds (1) and (2) were soluble in water. They were tethered onto oxidized polyglucose (PG) at ~ 20 wt% concentration via imine linkages susceptible to hydrolysis. In vitro release at 37 °C into phosphate buffer showed that around 75% of both the compounds was released in 24 h. The parent compounds and their conjugates were evaluated for their anti-leishmanial activity both in vitro, and in vivo in a Leishmania donovani/Balb/c mice model. In vitro, the most active compound was (1) with an IC50 value of 5.67 ± 0.0.44 μg/mL against axenic and 8.34 ± 0.74 μg/mL against intramacrophage amastigotes. In vivo, both compounds (1) and (2) were less active at a dose of 10 mg/kg body weight showing a reduction in parasite burden of 30.2 and 21.7% respectively. The conjugates of (1) and (2) with PG were inactive in vivo. Probably, much higher concentrations of the conjugates and prolonged treatment times are necessary for potential anti-leishmanial activity. 2-n-propylquinoline (2-PQ) is a natural compound having anti-leishmanial activities by the oral route but with a rapid elimination rate. 2-PQ is insoluble in water and therefore cannot be administered intravenously. As 2-PQ has no functional groups, two amine derivatives of 2-PQ were synthesized and conjugated to oxidized polyglucose. The parent compounds and their conjugates were evaluated for their anti-leishmanial activity both in vitro, and in vivo in a Leishmania donovani/Balb/c mice model. The new compounds were less active compared to AmBisome® both in vitro and in vivo and the conjugates were not active. Possibly, multiple injections and prolonged treatment durations are necessary for better in vivo response.

Comparison of Biological Responses of Polymers Based on Imine and Disulfide Backbones for siRNA Delivery.

To achieve a successful delivery of siRNA by carriers in vivo, the degradation of polymers in response to tiny intracellular changes should be seriously considered. In addition, the balance between degradation and stability of polymers is another key point for high performance of carriers. In this study, imine and disulfide linkages, which are sensitive to pH changes and redox environment, respectively, were constructed as the main backbone of polymers to deliver siRNA at the intracellular and animal level. Comparisons were made between performances of these two different polymers. Both of the polymers synthesized here have good ability to condense siRNA. However, polyplexes formed by the imine backbone-based polymer (TPSP) showed a larger particle size and a higher zeta potential than that of the disulfide backbone-based polymer (DTDPS). Although both TPSP and DTDPS could deliver the target siRNA into 7721 cells, polyplexes formed by TPSP showed a higher silence efficiency in vitro and accomplished more accumulation in tumors. In conclusion, we believe TPSP is superior to be used for siRNA delivery and promises a potential for widespread use.

Delivery of Amonafide from Fructose-Coated Nanodiamonds by Oxime Ligation for the Treatment of Human Breast Cancer.

The introduction of a strategy toward polymer/nanodiamond hybrids with high polymer grafting density and accessible polymer structural characterization is of critical importance for nanodiamonds' surface modification and bioagent attachment for their biomedical application. Here, we report a glycopolymer/nanodiamond hybrid drug delivery system, which was prepared by grafting amonafide-conjugated glycopolymers onto the surface of nanodiamonds via oxime ligation. Poly(1-O-methacryloyl-2,3:4,5-di-O-isopropylidene-β-d-fructopyranose)-b-poly(3-vinylbenzaldehyde-co-methyl methacrylate), featuring pendant aldehyde groups, is prepared via RAFT polymerization. The anticancer drug amonafide is conjugated to the polymer chains via imine chemistry, resulting in acid-degradable imine linkages. The obtained amonafide-conjugated glycopolymers are subsequently grafted onto the surface of aminooxy-functionalized nanodiamonds via oxime ligation. The molecular weight of the conjugated polymers is characterized by size-exclusion chromatography (SEC), while the successful conjugation and corresponding grafting density is assessed by nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric aanalysis (TGA). Our results indicate that the mass percentage of amonafide in the polymer chains is around 17% and the surface density of polymer chains is 0.24 molecules/nm2. The prepared drug delivery system has a hydrodynamic size around 380 nm with low PDI (0.3) and can effectively deliver amonafide into breast cancer cell and significantly inhibit the cancer cell viability. In 2D cell culture models, the IC50 values of ND-Polymer-AMF delivery system (7.19 μM for MCF-7; 4.92 μM for MDA-MB-231) are lower than those of free amonafide (11.23 μM for MCF-7; 13.98 μM for MDA-MB-231). An inhibited cell viability of nanodiamonds/polymer delivery system is also observed in 3D spheroids' models, suggesting that polymer-diamonds hybrid materials can be promising platforms for breast cancer therapy.

Dextran- and Chitosan-Based Antifouling, Antimicrobial Adhesion, and Self-Polishing Multilayer Coatings from pH-Responsive Linkages-Enabled Layer-by-Layer Assembly

To meet the demand for more environmentally-friendly antifouling coatings, and to improve the fouling-resistant coatings with both ‘offense’ and ‘defense’ functionalities, polysaccharides (PSa)-based self-polishing multilayer coatings were developed for combating biofouling. Dextran aldehyde (Dex-CHO) and carboxymethyl chitosan (CMCS) were synthesized and alternatively incorporated via imine linkage into the multilayer coating in layer-by-layer (LbL) deposition. Surface plasmon resonance (SPR) technique was utilized to monitor the LbL assembly process. With increasing number of assembled bilayers, the antifouling performances against bovine serum albumin (BSA) adsorption, bacterial (S. aureus and E. coli) adhesion, and alga (Amphora coffeaeformis) attachment improved steadily. The self-polishing ability of the multilayer coatings was achieved via cleavage of pH-responsive imine linkage under acidic environments. As such, dense bacterial adhesion induced detachment of the outmost layer of the coatings. The...

Diaminomaleonitrile based chromo-fluorescent receptor molecule for selective sensing of Mn(II) and Zn(II) ions

A new class of chemosensors (L1-L3) based on sequential coupling of 2,3-diaminomaleonitrile, 4-formyl-1-substituted phenyl-pyrazole-3-carboxylate and 2-hydroxy-1-naphthaldehyde through imine linkages were successfully designed and synthesized. The synthesized chemosensors showed quick colorimetric response with excellent selectivity and sensitivity towards detection of Mn2+ and Zn2+ ions in preference of other cations. Along with this, chemosensors also serve as fluorescent turn ‘off’ and turn ‘on’ switch for detection of Mn2+ and Zn2+ ion respectively. Analytical characterization were evaluated using UV–vis titration, fluorescent titration and Job’s plot methods which revealed that sensor interact with Mn2+ and Zn2+ ion in 1:1 and 1:2 binding stoichiometry respectively.

Chiral 3D Covalent Organic Frameworks for High Performance Liquid Chromatographic Enantioseparation.

In spite of their great promise for enantioselective processes due to the rich host-guest chemistry, it remains a challenge to construct covalent organic frameworks (COFs) with chiral three-dimensional (3D) structures. Here we report bottom-up synthesis of the first example of 3D chiral COFs by imine condensation of an enantiopure 2-fold symmetric TADDOL-derived tetraaldehyde with a tetrahedral tetra(4-anilyl)methane. After postsynthetic oxidation of imine linkages, the framework is transformed into an amide-linked COF with retention of crystallinity and permanent porosity as well as enhanced chemical stability. The resultant isostructural COFs feature a 4-fold interpenetrated diamondoid open framework with tubular channels decorated with chiral dihydroxy auxiliaries. Both COFs can be used as chiral stationary phases for high performance liquid chromatography to enantioseparate racemic alcohols, and the oxidized COF shows superior separation performance compared to the pristine framework.

Study of Schiff base formation between dialdehyde cellulose and proteins, and its application for the deproteinization of crude polysaccharide extracts

Dialdehyde cellulose (DAC) was produced by the selective oxidation of pulp fibers, and was used to bind bovine serum albumin (BSA) by forming Schiff bases. The best binding conditions were as follows: a content of DAC aldehyde groups of 5.15 ± 0.13 mmol/g, a reaction time of 4 h, a reaction temperature of 40 °C, an initial medium pH of 8.0, and a DAC/BSA ratio of 1:50 (g/mL). After the binding of BSA, the FT-IR intensity of the characteristic absorption band (CO) of DAC was weakened, which indicated the formation of imine linkages. The Schiff bases formed between DAC and BSA gave the resulting material a higher thermal stability than that of DAC, and the slender fibers were twisted out of shape. Based on this Schiff base reaction, three different crude polysaccharide solutions (CPSs) were treated with DAC to deproteinize them under best bonding conditions. The results revealed that the DAC method had a higher deproteinization efficiency and polysaccharide recovery than the classical Sevag method. Therefore, the DAC treatment may be an ideal technique for the removal of proteins from CPSs for large-scale purification of polysaccharides.

Transient imines as 'next generation' directing groups for the catalytic functionalisation of C-H bonds in a single operation.

C-H functionalisation promises a paradigm shift in synthetic planning. However, the additional steps often required to install and remove directing groups currently detract from the efficiency. The strategy of reversible installation of a directing group via an imine linkage has recently emerged, with the imine formed and hydrolysed in situ. Such transient directing groups can promote transition metal catalysed functionalisation of unactivated C-H bonds of aldehydes, ketones and amines. This approach removes additional steps usually required for covalent directing groups and can use catalytic quantities of the imine forming component. This review updates the rapidly developing field of transient directing groups for C-H functionalisation on sp2 and sp3 carbon centres, to form new C-C and C-X bonds. We focus on the structures of the transient directing groups as mono or bidentate coordinating groups for various metal catalysts.

Naked eye detection of ammonia gas using diphenylamine based conjugated polymers

Donor acceptor conjugated polymer containing imine linkage in the back bone (P1) was designed and synthesized by copolymerization of Octyl diphenylamine dialdehyde with p-diaminodiphenyl. The structure of monomers and polymer was characterized using various techniques. The optical property of the polymer solution and thin film was done using UV-Visible spectroscopic technique. The resistivity of the polymer was measured using Agilent B1500A. The Polymer film showed excellent response to ammonia gas. The color change can be visualized by naked eye which is reversible on exposure to acid. The response in terms of change in electrical resistivity on exposure to ammonia vapors was reported

Drug delivery systems based on biocompatible imino-chitosan hydrogels for local anticancer therapy

A series of drug delivery systems were prepared by chitosan hydrogelation with citral in the presence of an antineoplastic drug: 5-fluorouracil. The dynamic covalent chemistry of the imine linkage allowed the obtaining of supramolecular tridimensional architectures in which the drug has been homogenously dispersed. Fourier-transform infrared spectroscopy (FTIR), wide-angle X-ray diffraction (WXRD) and polarized light microscopy (POM) measurements were used in order to follow the hydrogelation and drug encapsulation processes. The ability of the prepared systems to release the drug has been investigated by UV–Vis spectroscopy using a calibration curve and by fitting the results with different mathematic models. To mimic the behavior of the hydrogel matrix in bio-environmental conditions in view of applications, their enzymatic degradability was monitored in the presence of lysozyme. The in vivo side effects of the systems, in terms of their influence on the blood elements, biochemical and immune parameters were monitored on white Swiss mice by intraperitoneal administration of the injectable obtained hydrogels. All the characteristics of the obtained systems, such as micro-porous morphology, uniform drug encapsulation, enzymatic degradability, lack of side effects, other than the one of the drug itself, along with their ability to release the drug in a sustained manner proved that these material meet the requirements for the development of drug delivery systems, making them suitable for being applied in intraperitoneal chemotherapy.

Precise Assembly of Synthetic Carriers of siRNA through a Series of Interlocked Thermodynamically Self‐Regulated Processes

AbstractA synthetic carrier of small interfering RNA (siRNA) with customizable size, pH‐responsive degradability and optimized surface population of cell‐targeting agents is constructed precisely through a series of interlocked thermodynamically self‐regulated processes. This system consists of a unimolecular polyplex core formed from each individual molecule of networked cationic polymer and a multi‐functional shell assembled from a rationally designed triblock copolymer. The core‐forming polymer of defined size is synthesized via Zeta potential‐regulated condensation of branched and linear multi‐amine bearing oligomers through pH‐responding imidazole conjugated imine linkages. The shell‐forming copolymer consists of a multi‐carboxyl saccharide end block to guide to the surface assembly, a hydrophobic central block to form an encapsulating layer, and a poly(ethylene glycol) end block equipped with a highly selective active ester for “hooking” various cell‐targeting agents. Animal assays confirm the flexibility and convenience of this system to equip with selected functional components for systemic delivery of siRNA.

Synthesis and evaluation of anti-fungal activities of sodium alginate-amphotericin B conjugates

Sodium alginate (SA) was oxidized using periodate and amphotericin B (AmB) was conjugated via imine and amine linkages to the oxidized alginate. Oxidization drastically reduced the molecular weight (MW) of the alginate. The conjugates were highly water-soluble to the extent of 1000mg/mL making them useful for therapeutic applications. SA-AmB conjugates derived from 20 and 50% oxidized alginate were non-toxic to HEK 293T and RAW 264.7 cell line at 100μg/mL and was also non-hemolytic to human blood at 100μg/mL. In vitro release of AmB into phosphate buffer from the imine conjugates was negligible with less than 0.2% of the drug released in 48h. Capping of residual aldehyde handles using 2-ethanolamine or glycine resulted in increased release of the drug in vitro. Injectable gels of gelatin crosslinked with oxidized alginate incorporating the SA-AmB conjugates as well as AmB were also fabricated and drug release was examined. In vitro release from the gel discs showed that AmB was released to the extent of 15–20% in 2days. The SA-AmB conjugates showed potent anti-fungal activity against C. albicans, C. neoformans and C. parapsilosis. The injectable gels seem to have potential for prolonged release of AmB when implanted.

A simple approach to design chitosan functionalized Fe3O4 nanoparticles for pH responsive delivery of doxorubicin for cancer therapy

The use of magnetic nanoparticles (MNPs) in cancer therapy offer many advantages due to their unique size, physical and biocompatible properties. In this study we have developed a formulation, comprising of anti-cancer drug doxorubicin (Dox) conjugated to iron oxide nanoparticles via a pH sensitive imine linker. Different amounts of chitosan functionalized superparamagnetic iron oxide nanoparticles (Fe3O4-CHI) were synthesized in-situ by a simple hydrolysis method at room temperature. The synthesized nanoparticles were well characterized by TEM, Zeta Potential, TOC, XPS, TGA and VSM for their physicochemical properties. Dox was conjugated to the Fe3O4-CHI nanoparticles via a glutaraldehyde cross linker with the imine (CN) bond, which is sensitive to cleavage in the pH range of 4.4–6.4. The synthesized Fe3O4-Dox nanoparticles exhibited enhanced drug release in lower pH conditions which mimics the tumor microenvironment or intracellular organelles such as endosomes/lysosomes. The cell uptake and therapeutic efficacy of Fe3O4-Dox nanoparticles carried out in ovarian cancer cell (SK-OV-3) and breast cancer cell line (MCF7) showed improved therapeutic efficacy of Dox by nearly four-fold with Fe3O4-Dox nanoparticles.

Observation of disordered mesomorphism in three-ring-based highly polar bent-core molecules: design, synthesis and characterisation

ABSTRACTResearch on low-temperature polar bent-core nematogens having lower molecular weight has gathered appreciable momentum by virtue of their significance in potential applications. However, the lack of availability and easy-to-perform synthesis processes appears to be the bottleneck towards their fabrication and thereby limiting their possible device applications. Hence, we have designed a new class of achiral symmetrical three-ring-based-bent shaped molecules incorporating an imine and ester linkage at the molecular bend with highly polar nitro/cyano terminal moiety exhibiting low-temperature nematic mesmorphism. The occurrence of disordered nematic mesomorphism has been confirmed by optical texture, differential scanning calorimetry scan and X-ray diffraction measurement. Dielectric spectroscopy and electro-optical investigation has also been carried out intending towards the potential applicability of the materials. Density functional theory analyses at the molecular level provide valuable information regarding the formation of the nematic mesophase and various parameters of the molecular spatial arrangement. Polarising optical microscopy study reveals the easy of alignment of these types of polar bent-core materials upon glass surface suitable for liquid-crystal-based sensing applications. Formation of mesophase with such a small bent molecule is rather difficult but we have successfully demonstrated the existence of disordered nematic mesomorphism at relatively low temperature.

A biomimetic and pH-sensitive polymeric micelle as carrier for paclitaxel delivery.

As nano-scale drug delivery systems, smart micelles that are sensitive to specific biological environment and allowed for target site-triggered drug release by reversible stabilization of micelle structure are attractive. In this work, a biocompatible and pH-sensitive copolymer is synthesized through bridging poly (2-methacryloyloxyethyl phosphorylcholine) (PMPC) block and poly (D, L-lactide) (PLA) block by a benzoyl imine linkage (Blink). Biomimetic micelles with excellent biocompatibility based on such PLA-Blink-PMPC copolymer are prepared as carriers for paclitaxel (PTX) delivery. Due to the rapid breakage of the benzoyl imine linkage under acidic condition, the micelle structure is disrupted with accelerated PTX release. Such pH-sensitive triggered drug release behavior in synchronization with acidic conditions at tumor site is helpful for improving the utilization of drug and facilitating antitumor efficacy. These micelles can be used as promising drug delivery systems due to their biocompatible and smart properties.

Supramolecular hybrid of ZnO nanoparticles with benzimidazole based organic ligand for the recognition of Zn 2+ ions in semi-aqueous media

Abstract Zinc oxide (ZnO) nanoparticles were decorated with benzimidazole based organic ligand ( L ) to form supramolecular hybrid for the recognition of Zn 2+ in HEPES-buffered DMSO/H 2 O (7:3; v/v) solvent media using absorption spectroscopy. The resultant hybrid compound ( N ) showed the shifting of imine linkage bands ( CH N ) in IR spectra due to the chemical attachment of organic ligand ( L ) on ZnO surface. The coating of organic ligand on the surface of ZnO was also confirmed with energy-dispersive x-ray (EDX) analysis. The resultant hybrid compound ( N ) exhibited high selectivity towards Zn 2+ ions over other metal ions in HEPES-buffered DMSO/H 2 O (7:3; v/v) solution. The absorption peak intensity for Zn 2+ ions was observed at 560 nm (1 nM), which was quenched by the addition of mole concentration and the peak shifted toward lower wavelength region at 442 nm (50 nM) resulted the development of hybrid chemosensor with a detection limit of 4.09 nM.