Imaging Spectroscopy Research Articles

Cyclodextrin-based nanosponges for the dispersive-solid phase extraction of pesticides from environmental waters

In this work, harmless, biodegradable, and low-cost cyclodextrin-based nanosponges (CDNS) were synthetized with an optimized green approach based on the use of a natural cyclodextrin (α-CD, β-CD) as the monomer, citric acid as the cross-linker, and NaH2PO4 as the catalyst. The resulting materials were studied using thermal analysis (TGA, DSC), IR spectroscopy, and SEM imaging. For the first time, α-CDNS and β-CDNS were successfully applied for the dispersive-solid phase extraction (d-SPE) from surface waters of eleven pesticides, including achiral compounds (ametocradin, methoxyfenozide, tebufenozide, pyraclostrobin, spiromesifen), optical stereoisomers (mandipropamid, penconazole, difenoconazole, pyriproxyfen, hexythiazox) and geometric stereoisomers (dimetomorph). The extracts were analysed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). A cholesteryl-bonded silica column allowed the baseline separation of the geometric stereoisomers of dimethomorph, while a polysaccharide-based column was effective in the separation of the optical stereoisomers. Although no nanosponge displayed stereo-discrimination, all of them provided absolute recoveries depending on the analyte logP, steric hindrance, and spike level. In particular, β-CDNS gave yields between 26 and 93 %, and intra-day and inter-day precision of 2–9 % and 4–18 %. Limits of detection and limits of quantitation were 0.05–40 ng/L and 0.2–120 ng/L, respectively. After validation, the d-SPE-HPLC-MS/MS method was applied to the analysis of water samples from the Tiber River: ametoctradin, mandipropamid, penconazole, pyraclostrobin and pyriproxyfen were detected at concentrations between 16 and 43 ng/L. Last but not least, recycling tests have proved that CDNSs can be reused three times with unvaried extraction yields.

225 Repair of oxidized hair fiber structure analyzed by infrared imaging spectroscopy

225 Repair of oxidized hair fiber structure analyzed by infrared imaging spectroscopy

Photodissociation Spectroscopy and Photofragment Imaging of the Mg+(Benzene) Complex

Photodissociation Spectroscopy and Photofragment Imaging of the Mg⁺(Benzene) Complex

Optimization of sandstone processing waste, electric arc furnace slag, and fly ash-based ternary blended eco-friendly geopolymers.

Over the years, ordinary Portland cement (OPC) has been used to meet growing demands of land and housing facilities arising out of population overburdens. It is well documented that OPCs, besides their outstanding qualities as building materials, are also significant contributors to global greenhouse gases (GHG). Consequently, recent years have noticed an emerging interest in the search for alternatives to Portland cement-based binders. Geopolymers are well-suited to serve this purpose owing to their superior attributes and low CO2 emissions compared to conventional cement. Still, the industrialization of geopolymers has not reached a meaningful value due to the prevailing fundamental barriers involving the requirement of corrosive environments and intensive heat-curing regimes in post-fabrication processes. The current study investigates the viability of using synergistic mixtures based on stone residues, pulverized ash, and steel slags in fabricating geopolymer composites cured at ambient temperature with reduced ingestion of alkalis. A comprehensive assessment of the engineering, mineralogical, and microstructural characteristics was performed in terms of setting times, physico-mechanical, durability, non-destructive, and analytical tests. Further, a scaled-down approach was utilized to evaluate the feasibility of the designed composites as construction entities. The incorporation of SW (10-40%) prolonged the setting periods (~ 150min.) and abridged the engineering properties of the ternary pastes collectively by 127% due to silica coalescences. Besides, replacements of stone residues with FA (20-30%) and EAF (30-60%) improved the blend performance due to Ca and Al assimilations. All the developed composites satisfied the acclamations for OPC grade 33, CEM V class 32.5N, and OPC Type - I suggested by IS, EN, and ASTM standards, respectively, with matrices constituting CASH-CSH-NASH-(N,C)-A-S-H type gelation complexes identified by the X-ray, infrared, and electron imaging spectroscopic analysis. In addition, a cumulative deficit of about 60-90% was observed in energy and carbon footprints relative to OPCs, indicative of the binders' sustainability traits.

Vibrational and Magnetic States of Point Defects in Bilayer MoSe2.

Defects in two-dimensional materials profoundly impact the physicochemical properties of the systems, whose characterization is highly desirable at the atomic scale. Here, using spectroscopic imaging scanning tunneling microscopy, we elucidate the vibrational and magnetic states of MoSe antisite and VMo vacancy with different charge states embedded in ultrathin MoSe2 bilayers supported on graphene substrate. Stringent vibronic states with multimode coupling are resolved on the defects. The spectral intensities are tunable with the electron tunneling rates and well-reproduced by theoretical modeling. Moreover, first-principles calculations suggest that the defects host a local magnetic moment of 2 μB in their neutral state, which is directly confirmed by our spin-flip inelastic electron tunneling spectroscopy. Our study deepens the understanding of defect properties and paves the way of defect-engineering material functionalities and spin-catalytic applications.

Multi-scale CLEAN for Fourier-based hard x-ray solar imaging

Abstract Multi-scale deconvolution is an ill-posed inverse problem in imaging, with applications ranging from microscopy, through medical imaging, to astronomical remote sensing. In the case of high-energy space telescopes, multi-scale deconvolution algorithms need to account for the peculiar property of native measurements, which are sparse samples of the Fourier transform of the incoming radiation. The present paper proposes a multi-scale version of CLEAN, which is the most popular iterative deconvolution method in Fourier-based astronomical imaging. Using synthetic data generated according to a simulated but realistic source configuration, we show that this multi-scale version of CLEAN performs better than the original one in terms of accuracy, photometry, and regularization. Further, the application to a data set measured by the NASA Reuven Ramaty High Energy Solar Spectroscopic Imager shows the ability of multi-scale CLEAN to reconstruct rather complex flaring topographies.

Rapid Water Quality Mapping from Imaging Spectroscopy with a Superpixel Approach to Bio-Optical Inversion

Rapid Water Quality Mapping from Imaging Spectroscopy with a Superpixel Approach to Bio-Optical Inversion

In Vitro Cell Model Investigation of Alpha-Synuclein Aggregate Morphology Using Spectroscopic Imaging.

Recently, it has been hypothesized that alpha-synuclein protein strain morphology may be associated with clinical subtypes of alpha-synucleinopathies, like Parkinson's disease and multiple system atrophy. However, direct evidence is lacking due to the caveat of conformation-specific characterization of protein strain morphology. Here we present a new cell model based in vitro method to explore various alpha-synuclein (αsyn) aggregate morphotypes. We performed a spectroscopic investigation of the HEK293 cell model, transfected with human wildtype-αsyn and A53T-αsyn variants, using the amyloid fibril-specific heptameric luminescent oligomeric thiophene h-FTAA. The spectral profile of h-FTAA binding to aggregates displayed a blue-shifted spectrum with a fluorescence decay time longer than in PBS, suggesting a hydrophobic binding site. In vitro spectroscopic binding characterization of h-FTAA with αsyn pre-formed fibrils suggested a binding dissociation constant Kd < 100 nM. The cells expressing the A53T-αsyn and human wildtype-αsyn were exposed to recombinant pre-formed fibrils of human αsyn. The ensuing intracellular aggregates were stained with h-FTAA followed by an evaluation of the spectral features and fluorescence lifetime of intracellular αsyn/h-FTAA, in order to characterize aggregate morphotypes. This study exemplifies the use of cell culture together with conformation-specific ligands to characterize strain morphology by investigating the spectral profiles and fluorescence lifetime of h-FTAA, based upon its binding to a certain αsyn aggregate. This study paves the way for toxicity studies of different αsyn strains in vitro and in vivo. Accurate differentiation of specific alpha-synucleinopathies is still limited to advanced disease stages. However, early subtype-specific diagnosis is of the utmost importance for prognosis and treatment response. The potential association of αsyn aggregates morphotypes detected in biopsies or fluids to disease phenotypes would allow for subtype-specific diagnosis in subclinical disease stage and potentially reveal new subtype-specific treatment targets. Notably, the method may be applied to the entire spectrum of neurodegenerative diseases by using a combination of conformation-specific ligands in a physicochemical environment together with other types of polymorphic amyloid variants and assess the conformation-specific features of various protein pathologies.

Structure, Optical and Electrical Properties of Nb(Zn) Doped Sol-Gel ITO Films: Effect of Substrates and Dopants.

We present comparative studies of sol-gel ITO multilayered films undoped and doped with Nb or Zn (4 at.%). The films were obtained by successive depositions of five layers using the dip-coating sol-gel method on microscopic glass, SiO2/glass, and Si substrates. The influence of the type of substrates and dopant atoms on the structure and optical properties of the sol-gel ITO thin films is examined and discussed in detail. XRD patterns of these layers showed a polycrystalline structure with an average crystallite size of <11 nm. Raman spectroscopy confirmed the chemical bonding of dopants with oxygen and showed the absence of crystallized Nb(Zn)-oxide particles, indicated by the XRD pattern. Spectroscopic Ellipsometry and AFM imaging revealed a clear dependence of the optical parameters and surface morphology of the ITO and ITO:Nb(Zn) thin films on the type of substrates and dopants. The analysis of the current-voltage and capacitance-voltage characteristics of the Al/ITO/Si structures revealed the presence of charge carrier traps in the ITO bulk and the ITO-Si interface. The densities of these traps are obtained and the character of the current transport mechanism is established.

Non-Ketotic Hyperglycemia Induced Hemichorea-Hemiballism May Represent Glioma-Like Pattern on Multimodal Magnetic Resonance Imaging: Can 1H Spectroscopy Help in the Differentiation?

Abstract Nonketotic hyperglycemia (NKH) induced hemichorea-hemiballismus (HC-HB) is an infrequent reversible condition observed in individuals with poorly controlled diabetes. In this report, we present a case of NKH-induced HC-HB exhibiting distinctive morphological and functional alterations on conventional magnetic resonance imaging (MRI), diffusion-weighted imaging (DWI), and 1H magnetic resonance spectroscopy (MRS), followed by subsequent monitoring. A 70-year-old male with a 20-year history of diabetes presented with severe unilateral involuntary movement. Computer tomography revealed heightened attenuation in the left putamen and caudate nucleus. The conventional MRI revealed the presence of focal T2-hyperintensity, noticeable mass effect, and ring-like enhancement, which are indicative of glioma. Additionally, the DWI showed unrestricted diffusion of water molecules within the lesion. MRS analysis further demonstrated significantly elevated lactate (Lac) and lipids (Lip), minimal increased choline (Cho), basically stable creatine (Cr), and modest decreased N-acetylaspartate (NAA) levels (which remained larger than both Cho and Cr peaks), leading to a diagnosis of NKH-induced HC-HB. This report emphasizes the significance of acknowledging that NKH-induced HC-HB can manifest with imaging features that bear resemblance to those of glioma. The presence of a slightly elevated Cho/NAA ratio alongside a notable increase in Lac/Lip peak on MRS may aid in ruling out neoplastic conditions.

Plasma p-tau181 and GFAP reflect 7T MR-derived changes in Alzheimer's disease: A longitudinal study of structural and functional MRI and MRS.

Associations between longitudinal changes of plasma biomarkers and cerebral magnetic resonance (MR)-derived measurements in Alzheimer's disease (AD) remain unclear. In a study population (n=127) of healthy older adults and patients within the AD continuum, we examined associations between longitudinal plasma amyloid beta 42/40 ratio, tau phosphorylated at threonine 181 (p-tau181), glial fibrillary acidic protein (GFAP), neurofilament light chain (NfL), and 7T structural and functional MR imaging and spectroscopy using linear mixed models. Increases in both p-tau181 and GFAP showed the strongest associations to 7T MR-derived measurements, particularly with decreasing parietal cortical thickness, decreasing connectivity of the salience network, and increasing neuroinflammation as determined by MR spectroscopy (MRS) myo-inositol. Both plasma p-tau181 and GFAP appear to reflect disease progression, as indicated by 7T MR-derived brain changes which are not limited to areas known to be affected by tau pathology and neuroinflammation measured by MRS myo-inositol, respectively. This study leverages high-resolution 7T magnetic resonance (MR) imaging and MR spectroscopy (MRS) for Alzheimer's disease (AD) plasma biomarker insights. Tau phosphorylated at threonine 181 (p-tau181) and glial fibrillary acidic protein (GFAP) showed the largest changes over time, particularly in the AD group. p-tau181 and GFAP are robust in reflecting 7T MR-based changes in AD. The strongest associations were for frontal/parietal MR changes and MRS neuroinflammation.

Interface-Engineering-Induced C-C Coupling for C2H4 Photosynthesis from Atmospheric-Concentration CO2 Reduction.

Producing ethylene (C2H4) from carbon dioxide (CO2) photoreduction under mild conditions is primarily restricted by the difficulty of C-C coupling. Herein, we designed highly active metal atom clusters anchored on semiconductor nanosheet, which established heteroatom sites on the interface to steer C-C coupling, realizing air-concentration CO2 photoreduction into C2H4 in pure water for the first time. As an example, the Pd nanoclusters loaded on ZnO nanosheets are prepared, demonstrated by the X-ray photoelectron spectroscopy and high-angle annular dark-field image. In situ Fourier transform infrared spectroscopy confirms the C-C coupling step over the Pd-ZnO nanosheets, while quasi in situ X-ray photoelectron spectroscopy illustrates the active sites of Pd and Zn atoms on the Pd-ZnO nanosheets during CO2 photoreduction. Density functional theoretical calculations unveil the transition state energy barrier of C-C coupling of CO* and COH* intermediates are only 0.998 eV, hinting the easy C-C coupling to produce C2 fuels. Therefore, the Pd-ZnO nanosheets first realize C2H4 photosynthesis by atmospheric-concentration CO2 reduction with the formation rate of 1.03 μmol g-1 h-1, while the ZnO nanosheets only acquired the carbon monoxide product.

Comparative Quantitative and Discriminant Analysis of Wheat Flour with Different Levels of Chemical Azodicarbonamide Using NIR Spectroscopy and Hyperspectral Imaging.

This study investigated and comprehensively compared the performance of spectra (950-1660 nm) acquired respectively from NIR and HSI in the rapid and non-destructive quantification of azodicarbonamide (ADA) content (0-100 mg/kg) in WF and simultaneously identified WF containing excessive ADA (>45 mg/kg). The raw spectra were preprocessed using 14 methods and then mined by the partial least squares (PLS) algorithm to fit ADA levels using different numbers of WF samples for training and validation in five datasets (NTraining/Validation = 189/21, 168/42, 147/63, 126/84, 105/105), yielding better abilities of NIR Savitzky-Golay 1st derivative (SG1D) spectra-based PLS models and raw HSI spectra-based PLS models in quantifying ADA with higher determination coefficients and lower root-mean-square errors in validation (R2V & RMSEV), as well as establishing 100% accuracy in PLS discriminant analysis (PLS-DA) models for identifying excessive ADA-contained WF in each dataset. Twenty-four wavelengths selected from a NIR SG1D spectra in a 168/42 dataset and 23 from a raw HSI spectra in a 147/63 dataset allowed for the better performance of quantitative models in ADA determination with higher R2V and RMSEV in validation (R2V > 0.98, RMSEV < 3.87 mg/kg) and for discriminant models in WF classification with 100% accuracy. In summary, NIR technology may be sufficient if visualization is not required.

Clinical characterisation of creatine transporter deficiency associated with SLC6A8 gene variants

Objective: To analyze the clinical features of creatine transporter(CRTR) deficiency associated with SLC6A8 gene variants. Methods: The clinical data (clinical presentation, brain imaging, creatine metabolism test and gene variants) of 5 patients admitted to Beijing Children's Hospital, Capital Medical University and diagnosed with CRTR deficiency associated with SLC6A8 gene variants from January 2016 to June 2024 were retrospectively analyzed. Results: A total of 5 patients, all male, presented with the onset of the disease at 1 year and 1 month old to 1 year and 10 months old, and diagnosis at 1 year and 3 months to 9 years old. All patients exhibited varying degrees of intellectual and physical developmental deficits, with autistic-like behaviors present in 2 patients and convulsive episodes present in 4 patients. The brain magnetic resonance imaging, of all 5 patients showed either poor or slightly delayed myelination. Additionally, the brain magnetic resonance spectroscopy (MRS) imaging of 5 patients all showed decreased creatine peaks, and 4 patients had elevated urinary creatine levels indicated by blood and (or) urine creatine metabolism tests. There were 3 patients completed blood creatinine tests, all indicating reduction. Whole-exome sequencing was conducted on all 5 patients, and 4 unreported novel variants were identified: c.371G>C, c.1017-1G>A, c.912+1G>T and c.1016+2T>A. Following treatment with creatine, arginine, and glycine supplementation, no significant advancement was observed in motor and language development. Conclusions: CRTR deficiency associated with SLC6A8 gene variants manifests as developmental delay with or without seizures, with atypical clinical features, the presence of decreased blood creatinine levels and elevated urinary creatine levels. Combined with brain MRS and genetic testing results, patients can be diagnosed.

Characterisation, comparison of surface topography and frictional resistance of zinc oxide- tin oxide nanocoated ceramic orthodontic brackets by radio frequency magnetron sputter coating method: An in vitro study

In recent years, orthodontic research has witnessed significant progress as it ventures into the exploration of nanoparticle coating to augment the surface properties of orthodontic appliances. The present study aimed to evaluate the surface characteristics, surface topography and frictional resistance (FR) of ceramic brackets (CB) nanocoated with zinc oxide- tin oxide (ZnO-SnO) by radio frequency magnetron sputter coating method.26 polycrystalline maxillary canine CB, split into two groups, were used in the current in vitro investigation. Group A of the RF magnetron sputter coating method was used to coat ZnO-SnO nanoparticles (Nps) on brackets, while group B of the process used uncoated brackets. Following coating, brackets underwent EDAX and SEM imaging. Atomic force microscopy (AFM) was used to assess the surface topography, and frictional resistance (FR) was also examined. An analysis of the data was conducted using SPSS (Version 23.0). An independent parametric t-test was used to compare the results between the groups.Brackets coated by RF sputter coating method had a porous and aggregated morphology when viewed under SEM. EDAX spectroscopy images showed uncoated brackets presented aluminium, oxygen, silica and calcium signal peaks at 60.83 wt %, 13.43 wt %, 24.57 wt % and 1.17 wt % respectively while the coated brackets showed signal peaks of zinc, oxygen, silica and tin at signal peaks of 20.98 wt %, 54.85 wt %, 10.52 wt % and 13.65 wt %. Groups A and B showed a surface roughness (SR) of 180.62 ± 9.49 nm and 316.77 ± 14.10. A statistically significant difference was observed in the SR between the 2 groups (p=0.00). The mean FR were higher for uncoated brackets (8.18 ± 0.76) p=0.00.Zn-SnO2 Nps were effectively coated onto ceramic brackets through the RF magnetron sputter coating technique. In comparison to uncoated brackets, the coated brackets exhibited a lower FR and SR.

P-d Correlation-Determined Charge Order Stiffness and Corresponding Quantum Melting in Monolayer 1T-TiSe2.

1T-TiSe2, a promising candidate of the sought-after excitonic insulator, possesses an enigmatic charge density wave (CDW) order of which the microscopic origin is formidable to settle owing to the chicken-and-egg entanglement between the electron and lattice degrees of freedom. Its CDW experiences an intriguing but elusive quantum melting and eventually enters the superconducting phase under metal intercalation, suggesting the possible role of melted-order fluctuation in gluing the electron paring. Employing the spectroscopic imaging scanning tunneling microscope (STM), we access the pure electronic behavior by visualizing the CDW melting process of monolayer 1T-TiSe2 in both the space and energy-band dimensions. In real space, the native lattice imperfections disturb the local order parameter and stimulate the melting of CDW. In energy-band space, different states exhibit varying stiffness against the melting stimuli, yielding distinctive melted textures. The evolution of CDW topological defects and the structure factor in the quantum melting process provide a straightforward avenue to evaluate the CDW coherency, which shows that the CDW stiffness scales with the strength of the p-d Coulomb correlation. Our study reveals the quantum melting of CDW with an altering band-orbital-correlation character and puts compelling emphasis on the indispensable role of excitonic interaction in stabilizing the charge order of monolayer TiSe2.

First-in-Human: Simultaneous Hyperpolarized 1-13C-Pyruvate Magnetic Resonance Spectroscopy and 18F-FDG PET (hyperPET) Imaging of a Patient With Lymphoma.

Hyperpolarized 1-13C-pyruvate magnetic resonance spectroscopy (MRS) and MRS imaging (MRSI) offer noninvasive and real-time direct assessment of the altered metabolism of cancer cells known as the Warburg effect-a key hallmark of cancer. When combined with simultaneously acquired 18F-FDG PET in a PET/MR scanner, coined hyperPET by us, this dual-modality may unveil cancer-type specific glucose metabolic phenotypes with potential implications for patient prognostication, treatment-response assessment, and prediction. We here present the first human data of simultaneously acquired hyperpolarized MRS/MRSI and PET performed in a PET/MR scanner-and the first human hyperpolarized MRS/MRSI data from a patient with lymphoma.

Prediction of genetic profile of breast carcinoma on MRI using a combination of DCE‐MRI, DWI, and MR spectroscopy: A prospective observational study

AbstractBackgroundClassification of breast cancer based on gene expression has emerged as the standard approach in its management, owing to the distinct prognoses and treatment responses observed among different subtypes. The aim of this study was to prospectively assess the imaging features of the molecular subtypes of breast cancer using multiparametric magnetic resonance imaging (mMRI) with the combined assessment of dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI), diffusion‐weighted imaging (DWI), and MR spectroscopy (MRS).MethodsThis was a prospective observational single‐center cohort study, which included women with BI‐RADS 4−5 lesions on mammography/ultrasound (US) who subsequently underwent 1.5 T MRI (encompassing DCE‐MRI, DWI, and MRS). The histological subtypes of breast cancer were assessed. Estrogen receptor (ER), progesterone receptor (PR), Ki‐67 status, and human epidermal growth receptor‐2 (HER2) expression, assessed by immunohistochemistry (IHC), defined four molecular subtypes: luminal A, luminal B, HER2‐enriched (Her2en), and triple‐negative breast carcinoma (TNBC). Statistical associations between the four molecular subtypes and MRI features were investigated.ResultsFifty patients were included in the study. Circumscribed margins were significantly correlated with triple‐negative tumors compared to others (78% versus 6%, p &lt; 0.001). Spiculated margins were observed in non‐triple negative tumors. Rim enhancement was significantly correlated to triple‐negative tumors compared to all other subtypes (71.4% versus 25%, p = 0.035). Mean apparent diffusion coefficient (ADC) values were significantly lower for luminal subtypes compared to non‐luminal subtypes (p &lt; 0.001). The total choline (tCho) signal‐to‐noise ratio (SNR) was higher in triple‐negative tumors. A combined algorithm using DCE‐MRI, DWI, and MRS can predict TNBC and Her2en with specificity of 86.6% and 100%, respectively, and sensitivity of 100% and 85.37%, respectively.ConclusionThe combination of mMRI with DCE‐MRI, DWI, and MRS can accurately differentiate the molecular subtypes of breast carcinoma.

Coherent Raman microscopy visualizes ongoing cellular senescence through amide I peak shifts originating from β sheets in disordered nucleolar proteins

Cellular senescence occurs through the accumulation of many kinds of stresses. Senescent cells in tissues also cause various age-related disorders. Therefore, detecting them without labeling is beneficial for medical research and developing diagnostic methods. However, existing biomarkers have limitations of requiring fixation and labeling, or their molecular backgrounds are uncertain. Coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging is a novel option because it can assess and visualize molecular structures based on their molecular fingerprint. Here, we present a new label-free method to visualize cellular senescence using CARS imaging in nucleoli. We found the peak of the nucleolar amide I band shifted to a higher wavenumber in binuclear senescent cells, which reflects changes in the protein secondary structure from predominant α-helices to β-sheets originating from amyloid-like aggregates. Following this, we developed a procedure that can visualize the senescent cells by providing the ratios and subtractions of these two components. We also confirmed that the procedure can visualize nucleolar aggregates due to unfolded/misfolded proteins produced by proteasome inhibition. Finally, we found that this method can help visualize the nucleolar defects in naïve cells even before binucleation. Thus, our method is beneficial to evaluate ongoing cellular senescence through label-free imaging of nucleolar defects.

Ground and Low‐Lying Excited States of Nickel Monocarbonyl Obtained by Anion Photoelectron Velocity Map Imaging Spectroscopy

AbstractThe photodetachment of nickel monocarbonyl anion was investigated with photoelectron velocity map imaging spectroscopy and analyzed using the existing and new theoretical calculations. The photodetachment experiment conducted at three detachment photon energies (i. e. 1064, 532, and 355 nm) revealed a wealth of spectroscopic information about both the ground state and low‐lying excited states of NiCO. Franck‐Condon simulations were performed to assist the spectral assignment of the vibrationally resolved ground‐state transition. The electron affinity of NiCO is measured to be 0.775±0.002 eV. Three stretching vibrational modes were determined to be activated upon photodetachment, with frequencies of 2016±100, 597±10, and 564±10 cm−1. The higher‐energy electronic transitions were divided into two congested spectral bands, falling in the ranges of 1.8~2.8 and 2.8~3.5 eV, respectively. The current VMI provided valuable benchmark data for the theoretical calculations on the nickel carbonyls.