Viral ImmunologyVol. 30, No. 7 CorrectionFree AccessCorrection to: Viral Immunol 2017;30:298–301. DOI: 10.1089/vim.2016.0152is erratum ofIL17A Polymorphism Is Not Associated with Human T-Lymphotropic Virus 1-Associated Myelopathy/Tropical Spastic ParaparesisPublished Online:1 Sep 2017https://doi.org/10.1089/vim.2016.0152.correctionAboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail In the May 2017 issue of Viral Immunology (vol. 30, no. 4; 298–301), in the article titled “IL17A Polymorphism Is Not Associated with Human T-Lymphotropic Virus 1-Associated Myelopathy/Tropical Spastic Paraparesis” by Neco et al., experiments were performed using Taqman probes and real-time polymerase chain reaction (real-time PCR) technique. However, the acronym RT-qPCR was used in the article, which the authors believe is not adequate to define this technique. The authors prefer to state they performed real-time PCR, and so have made the following corrections:In the article Abstract, the sentence was changed to The single nucleotide polymorphism genotyping was carried out by real time PCR using TaqMan® probes. In the Materials and Methods section, the sentence was changed to The G-197A polymorphism genotyping was performed by real time PCR using the TaqMan® SNP Genotyping Assay Kit (ID C_15879983_10), according to the manufacturer's instructions (Applied Biosystems, ABI, Foster City, CA), under the following conditions: 10 min at 95°C, followed by 40 cycles (15 sec at 92°C followed by 60 sec at 60°C) and 30 sec at 60°C.By way of explanation, older articles use the term RT-PCR referring to real-time PCR, while current articles refer to reverse transcription PCR. Thus, to avoid confusion with acronyms and following the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) (1), the authors would like to explain each term below:qPCR (Quantitative PCR): Also known as real-time polymerase chain reaction, in this method the input material is DNA, and its quantification is performed after each amplification cycle, usually by using fluorescent probes.RT-PCR (Reverse Transcription-PCR) and RT-qPCR (Quantitative Reverse Transcription PCR [RT-qPCR]): The input material is RNA, which is transcribed into complementary DNA (cDNA) by reverse transcriptase enzyme. In this technique, cDNA is used as a template for a qPCR reaction.References1 Bustin SA, Benes V, Garson JA, et al. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009;55:611–622. Crossref, Medline, Google ScholarThe online version of this article has been corrected to reflect this change. References FiguresReferencesRelatedDetailsRelated articlesIL17A Polymorphism Is Not Associated with Human T-Lymphotropic Virus 1-Associated Myelopathy/Tropical Spastic Paraparesis1 May 2017Viral Immunology Volume 30Issue 7Sep 2017 InformationCopyright 2017, Mary Ann Liebert, Inc.To cite this article:Correction to: Viral Immunol 2017;30:298–301. DOI: 10.1089/vim.2016.0152.Viral Immunology.Sep 2017.545-545.http://doi.org/10.1089/vim.2016.0152.correctionPublished in Volume: 30 Issue 7: September 1, 2017PDF download