IgG In Plasma Cells Research Articles

Evaluation of the Status of Intraparenchymal Plasma Cells in Terms of IgG4 Expression in Patients Diagnosed With Idiopathic Granulomatous Mastitis

Background and Objectives Idiopathic granulomatous mastitis (IGM) is a chronic inflammatory lesion that mimics the clinical symptoms of carcinoma. Another type of chronic granulomatous mastitis is immunological mastitis, which is related to the infiltration of IgG4 plasma cells. This study aims to determine the status of IgG4 positive plasma cells in IGM samples. Subjects and Methods This is an analytical-cross-sectional study. 46 women diagnosed with IGM were selected for the study using census method. The percentage of IgG and IgG4 in plasma cells was determined based on immunohistochemical method. Age and type of surgery were also recorded. The data was entered into SPSS software, version 22 and analyzed using statistical tests. P<0.05 were considered statistically significant. Results The mean age of patients was 35.02±6.82 years. The mean percentage of IgG positive plasma cells among tissue inflammatory cells was 6923±23.42 and the mean percentage of IgG4 positive plasma cells was 23.39±22.33. Among infiltrated plasma cells, 1.89% were positive for IgG4 and the ratio of IgG4 to IgG in plasma cells was 0.91. Based on the results of Pearson correlation test, a positive and significant correlation was found between the percentage of IgG4 and IgG in plasma cells (r=0.600, P=0.004). Conclusion A significant percentage of infiltrated plasma cells are IgG4 positive, which can play a role in the development of IGM. Therefore, further investigation and comparison with immunological mastitis is recommended.

Expression and significance of IgG4 in inflammatory disease of nasal cavity and paranasal sinuses

To study the prevalence of IgG4-positive plasma cells in inflammatory disease of nasal cavity and paranasal sinuses and its association with IgG4-related sclerosing disease (IgG4-SD). The expression of IgG4 and IgG in plasma cells of 103 cases diagnosed as inflammatory disease of nasal cavity and paranasal sinuses with dense lymphoplasmacytic infiltrate was studied by immunohistochemistry (EnVision) and quantitatively analyzed by medical image analysis system. Immunohistochemical study showed marked infiltration by IgG4-positive plasma cells (>50 per high-power field) in 28 cases, moderate infiltration (30 to 50 per high-power field) in 23 cases, mild (10 to 29 per high-power field) in 30 cases and negative (<10 per high-power field) in 22 cases (P < 0.05). Twenty-two cases studied fulfilled the diagnostic criteria of IgG4-SD (>50 IgG4-positive plasma cells per high-power field and IgG4-to-IgG ratio > 40%), including 3 cases of chronic sinusitis (3/20), 3 cases of nasal polyps (3/18), 3 cases of inflammatory pseudotumor (3/17), 4 cases of fungal sinusitis (4/20), 1 case of rhinoscleroma (1/12), 7 cases of Wegener's granulomatosis (7/11) and 1 case of Rosai-Dorfman disease (1/2). Inflammatory disease of nasal cavity and paranasal sinuses fulfilling the diagnostic criteria IgG4-SD is not uncommon. Definitive diagnosis of IgG4-SD requires correlation with other clinical and laboratory findings. Some cases of unexplained inflammatory disease of nasal cavity and paranasal sinus may represent a member of the IgG4-SD spectrum. IgG4 carries diagnostic value in differential diagnosis of inflammatory disease occurring in nasal cavity and paranasal sinuses.

Immunohistochemical study of the local immune response to Fasciola hepatica in primarily and secondarily infected goats

The distribution of CD2, CD4, CD8, γ/δ T-lymphocytes, B-cells and IgG lambda-light chain (λ-IgG) containing cells were analysed in the inflammatory infiltrate associated to hepatic lesions and gallbladder (HL), and in hepatic lymph nodes (HLN) of goats primarily and secondarily infected with Fasciola hepatica. In the HL, CD2 and CD8 T-cells were more numerous ( p=0.01) in secondarily rather than in primarily infected goats, whereas CD4 T-lymphocytes were less numerous than CD8 and showed no significant change in both groups. The ratio CD4/CD8 was 0.66 and 0.39 for primarily and secondarily infected goats, respectively. In contrast, in the HLN, CD4 were more numerous than CD8 T-cells, the ratio CD4/CD8 was 2.0 in control, 1.5 and 1.3 in primary and secondary infections, respectively. γ/δ T-lymphocytes were scarce in the HL and moderate in the HLN of both primarily and secondarily infected animals. B-cells (IgM +, λ-IgG + or CD79 +) varied from scarce or moderate in the HL to abundant in the HLN, where CD79 +-cells were mainly located in lymphoid follicles and IgM and IgG in plasma-cells of the medullary cords, suggesting an intense local humoral immune response. However, this response did not prevent the hepatic damage in secondarily infected goats, in which hepatic lesions were more severe than in primarily infected ones.