Identification Of Vibrio Vulnificus Research Articles

The real-time PCR identification of Vibrio vulnificus from oyster samples from Black Sea region and imported origin

The real-time PCR identification of Vibrio vulnificus from oyster samples from Black Sea region and imported origin

Identification of Vibrio vulnificus from patients with otitis media and septicemia by PCR method

Sucrose-negative colonies were isolated on TCBS agar plate from otorrhea specimens from otitis media patient in the Adult Diseases Examination Center, Hirosaki Medical Association. The isolate was identified as Vibrio vulnificus by nested PCR method which amplify V. vulnificus-specific sequences directed against 23S rRNA genes. The PCR method was applied to identify 6 strains originally isolated from septicemia patients in Kurashiki Central Hospital and formerly identified as V. vulnificus by phenotypic characteristics. When examined by the API20E system, the above PCR confirmed-V. vulnificus isolates were correctly identified as V. vulnificus with % ID 99.8, though this gave 3 different profiles. Cytotoxin-hemolysin gene was detected in otorrhea strain as well as septicemia strains by PCR method. The above results suggest that PCR method targeted cytotoxin-hemolysin gene is suitable for rapid and accurate identification of the isolate, because the result is obtained in less than 4 h. To our knowledge this is the first report on the V. vulnificus infection in Aomori Prefecture and the isolation from otorrhea.

Identification of Vibrio vulnificus by Indirect Immunofluorescence

A rapid, sensitive, and specific indirect immunofluorescence (IIF) procedure is described for identifying Vibrio vulnificus. Reference antisera were prepared by vaccinating rabbits with surface antigen preparations of V. vulnificus, and the antisera were examined for the ability (1) to react with and serologically group 85 isolates of V. vulnificus grown in heart infusion broth, and (2) to detect V. vulnificus in tissue specimens from mice experimentally infected with a virulent isolate of the bacterium. The antisera detected 100% of the V. vulnificus isolates examined and gave false-positive results in approximately 0.9% of 445 IIF tests performed with non- V. vulnificus clinical isolates. V. vulnificus also was detected in frozen tissue sections from infected mice; however, the most easily observed positive results were obtained by examining V. vulnificus from lesion specimens and blood cultured briefly in heart infusion broth. The bacteria in 2-hr-old cultures of local lesions fluoresced brilliantly and were easily detectable. The IIF procedure could be of value in rapidly diagnosing fulminating and potentially fatal human disease caused by V. vulnificus.