Identification Of Potential Allergens Research Articles

Identification of potential allergens in larva, pupa, moth, silk, slough and feces of domestic silkworm (Bombyx mori)

The silkworm (Bombyx mori) is an important economic insect that can be used as food in many countries in Asia. However, silkworms and their metabolites are an important source of allergens, which can induce severe allergic reactions. So far, there are no systematic studies on the potential allergens in silkworm and its metabolites. These studies have important guiding significance for the prevention, diagnosis, and treatment of silkworm allergy. The aim of this study was to identify the potential allergens from larva, pupa, moth, silk, slough and feces of silkworm and analyze the sequence homology of silkworm allergens with other allergens identified in the Allergenonline database. We have found 45 potential allergens in silkworm. The results of the homology comparison suggested that silkworm allergens likely cross-react with those of Dermatophagoides farinae, Aedes aegypti, Tyrophagus putrescentiae, Triticum aestivum and Malassezia furfur.

Immune-analytical detection of the cross-reactive major cereal allergens

Investigation of allergens of celiac disease and cereal allergy has become important for scientific research. Immune-blotting is currently one of the most appropriate methods applied for the identification of potential allergens. The research was focused on the examination of in vitro allergenic activity of proteins in domestic wheat, triticale, rye, barley, rice, corn, buckwheat and amaranth cultivars. The IgA/IgE reactive epitopes of water/salt- and alcohol-soluble protein fractions, respectively, were detected with IgA antibodies of celiac and IgE antibodies of wheat allergic human sera. Further efforts were made to separate the alpha-amylase inhibitors from the water/salt-soluble protein fraction of wheat by DEAE-cellulose anion-exchange chromatography and to confirm their presence on native gel. The most frequently recognised IgE reactive proteins (27) by wheat allergic human sera were identified by liquid chromatography coupled with mass spectroscopy and accession to databank supply. The pepsin resistance of identified wheat allergens was assessed directly on PVDF membrane-blotted protein bands.

Specific IgE reactivity and identification of potential allergens in macadamia allergy

Specific IgE reactivity and identification of potential allergens in macadamia allergy

Identification of potential allergens in white oak ( Quercus alba) pollen by immunoblotting

Aqueous extracts of white oak pollen were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes. The nitrocellulose membranes were blocked with phosphate-buffered saline 15% nonfat dry milk, incubated with dilutions of sera from atopic or control subjects, and probed with a radiolabeled or peroxidase-labeled antihuman IgE. The IgE binding bands were detected by autoradiography or enzymatic reaction; 45 to 50 protein bands were observed in silver-stained gels. IgE from 30 of the 38 sera tested from oak-sensitive subjects bound to 23 bands with molecular weights (MWs) between 106 to 108 kd (band 1) and 13.2 to 15.2 kd (band 23). No band was recognized by sera of every patient. Band 5 (MW 74.0 to 77.9 kd) and band 21 (MW 16.2 to 17.7 kd) were recognized by 71% of the patients' sera. Multiple bands were recognized by 30% to 50% of the sera tested. All patients who were skin test positive to oak by prick testing had positive immunoblots. Of 12 patients positive by intradermal skin testing, only four patients had positive immunoblots. The average number of allergens recognized by a single patient was 6.6. The maximum number of allergens to which any individual reacted was 18; the minimum number was one. Extracts separated under nonreducing conditions resulted in aggregates that did not enter the polyacrylamide gel. Of the protein that did enter the gel, the higher MW species elicited banding patterns similar to patterns observed under reducing conditions, whereas lower MW IgE binding bands were lost. These data suggest that the extractable proteins of white oak pollen contain multiple proteins that are potentially allergenic.

Inhalant Allergens in Patients with Allergic Rhinitis in Riyadh

A group of allergen skin-test reagents were selected for study in Saudi patients from aerobiological and immunochemical identification of potential allergens in the atmosphere and from dust samples...