Identification Of Flavonol Glycosides Research Articles

Phytochemical analysis, antipropulsive and antilymphoma activities of leaves extract of Annona cherimola miller

Background: Diarrhea and cancer are significant health problems worldwide. Annona cherimola Mill. (Annonaceae) is a fruit tree extensively used in Mexican traditional medicine for the treatment of diarrhea and cancer. Objectives: The present work reports the identification of flavonol glycosides present in the ethanol crude extract using high-performance liquid chromatography with diode array detection (HPLC-DAD). Materials and Methods: The identification of flavonoids was made by comparing their retention times and ultraviolet spectra with those standards. The crude extract, dichloromethane, and aqueous residual (AR) fractions were tested for their antipropulsive and antilymphoma activities. Results: The results revealed high levels of three flavonoid glycosides (rutin, nicotiflorin, and narcissin), especially rutin (24.5 mg/g of extract). The antipropulsive and antilymphoma activities of the leaf extract and subsequent fractions showed that the ethanol extract of the leaves of A. cherimola is cursive and AR fraction exhibited the best biological effects in both assays. Conclusion: The HPLC-DAD method used in this work enables the determination of rutin as a major flavonol glycoside of the leaves of A. cherimola . In addition, it can be suggested that rutin may play an important role in the biological properties of A. cherimola.

Identification of flavonol glycosides in American cranberry fruit

Two flavonol glycosides, quercetin galactoside and quercetin arabinoside, have been identified in American cranberry fruit, as a complementary investigation of our previous study. The analysis processes included separation, hydrolysis and structure elucidation of flavonol glycosides. The separation of flavonol glycosides was carried out by solvent extraction, thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC). After hydrolysis of the obtained flavonol glycosides, flavonol aglycones and sugars were identified by HPLC and gas chromatography–mass spectrometry (GC–MS), respectively.

Identification of Flavonol Glycosides Using MALDI‐MS

Identification of Flavonol Glycosides Using <scp>MALDI</scp>‐<scp>MS</scp>

HPLC separation of flavonol glycosides in wines

Isocratic elution from a reversed-phase high performance liquid chromatographic column was used to separate several flavonol glycosides of wines after a preparative isolation which includes column, paper and thin-layer chromatographic procedures. The method has been applied to the separation and identification of flavonol glycosides in wines from different regions in Spain.