Identification Of Cyanide Research Articles

Identification of Cyanide in Sediment of River in Hulawa Village

(1) Background: mining activities in Hulawa Village have caused heavy metal contamination in the surrounding environmental ecosystem. This is caused by the waste from gold processing which is dumped directly into the river body. The purpose of this study was to determine the Cyanide content in the sediment river in Hulawa Village; (2) Methods: this research is a descriptive study, using a purposive sampling method, with the sampling location at the midpoint of the river. This research was conducted in August-September 2020. The sediment sampling technique used the JPHA guidelines, and the cyanide examination in the sediment used the AAS test; (3) Result: the sediment samples obtained were then taken to the WLN laboratory in Manado to be tested for cyanide content. The test results showed that the cyanide content in the sediment was <0.5 mg/kg; (4) Conclusion: apart from heavy metal Mercury (Hg), the river in Hulawa Village has also been polluted by heavy metal Cyanide (CN). Cyanide content is still less than 0.5 mg/kg due to its not yet intensive use. However, if used continuously by miners, the possibility of cyanide accumulating in large quantities cannot be avoided, and this can have a health impact on the people in Hulawa Village.

Mechanistic Studies on Tryptophan Lyase (NosL): Identification of Cyanide as a Reaction Product.

Tryptophan lyase (NosL) catalyzes the formation of 3-methylindole-2-carboxylic acid and 3-methylindole from l-tryptophan. In this paper, we provide evidence supporting a formate radical intermediate and demonstrate that cyanide is a byproduct of the NosL-catalyzed reaction with l-tryptophan. These experiments require a major revision of the NosL mechanism and uncover an unanticipated connection between NosL and HydG, the radical SAM enzyme that forms cyanide and carbon monoxide from tyrosine during the biosynthesis of the metallo-cluster of the [Fe-Fe] hydrogenase.

High resolution 19F{1H} nuclear magnetic resonance spectroscopy and liquid chromatography–solid phase extraction–offline 1H nuclear magnetic resonance spectroscopy for conclusive detection and identification of cyanide in water samples

We report herein a new, sensitive and efficient method for detection, identification and quantification of cyanide in water samples. Cyanide was converted (>95% yield) to its versatile and stable derivative, 1-cyano-2,2,2-trifluoro-1-phenylethyl acetate (CTPA).The crude reaction mixture was directly subjected to high resolution fluorine-19 nuclear magnetic resonance (19F{1H} NMR) spectroscopy. In order to do away with signal overlap and dynamic range problems associated with 1H NMR spectroscopy, liquid chromatography with UV detection hyphenated to online solid phase extraction (LC–UV–SPE) was performed. The trapped and enriched CTPA was thereafter subjected to offline 1H NMR spectroscopy. In this way the δ1H, δ19F spectral signatures and LC–UV retention time were used for specific detection and identification and quantification of cyanide. The three techniques (viz. LC–UV and LC–UV–SPE followed by offline 1H NMR and 19F{1H} NMR spectroscopy) demonstrated good linearity (r2>0.99), reproducibility (inter-day RSD: 1.43–1.89%, 2.60–2.80%, 1.42–1.60; intra-day: RSD 1.20–1.38%, 3.21–3.25%, 1.00–1.19%), accuracy (recoveries: 95.1–97.2%, 77.5–82.8%, 96.8–98.9%) and LODs (1.31μg/mL, 4.24μg/mL, and 2.14μg/mL) respectively. Total time required for the analysis was ∼3h. Utility of the method was demonstrated by the detection and identification of spiked water samples. Since the derivative CTPA was volatile, could also be analyzed by GC–MS and GC–FTIR instruments.

A Note on the Determination and Identification of Cyanide in Biologic Materials

Abstract A procedure is described for the determination of cyanide in biologic fluids. Distinction can be made between simple cyanides, ferrocyanides and ferricyanides, nitroprussides, and cyanogenetic substances, such as amygdalin. Traces of cyanogenetic substances resembling amygdalin could be detected in normal urine. Other types of cyanide-like material could not be detected.