Ideal Surface Research Articles

Aerodynamic optimisation of flat-upper-surface wing

Purpose The paper aims to optimise several concepts of the flat-upper-surface wing that could install the largest possible number of photovoltaic cells and test them in flight. A wing ideal flat upper surface was necessary to provide the same lighting conditions for each tested cell. Design/methodology/approach The optimised wings were built based on a developed family of airfoils having 75% of their upper surface flat. Within the developed parametric model of the wings, the design parameters described the spanwise distribution of base airfoils. Maximisation of the endurance factor was assumed as the main objective. The aerodynamic properties of optimised wings were evaluated using a panel method coupled with boundary layer analysis. Findings The paper proves that it is possible to design wings with 75% of their upper surface perfectly flat, which are also characterised by good aerodynamic properties. Practical implications The research conducted will allow designing an experimental unmanned aerial vehicle dedicated to investigating the properties of electrical propulsion systems at various altitudes. Data obtained in these investigations will help in the development of future generations of electric-propulsion aircraft. Originality/value The innovative wings, developed within the research are unique due to their unusual geometric and aerodynamic properties. They have 75% of their upper surface perfectly flat. That makes them ideal for testing various photovoltaic cells in flight. The biggest challenge was to design the wings so that their specific geometric features did not impair their aerodynamic properties. The paper proves that this challenge has been fully overcome.

Biological Characterization of Ti6Al4V Additively Manufactured Surfaces: Comparison Between Ultrashort Laser Texturing and Conventional Post-Processing.

Among Additive Manufacturing (AM) technologies, Laser Powder Bed Fusion (LPBF) has made a great contribution to optimizing the production of customized implant materials. However, the design of the ideal surface topography, capable of exerting the best biological effect without drawbacks, is still a subject of study. The aim of the present study is to topographically and biologically characterize AM-produced Ti6Al4V ELI (Extra Low Interstitial)samples by comparing different surface finishing. Vertically and horizontally samples are realized by LPBF with four surface finishing conditions (as-built, corundum-sandblasted, zirconia-sandblasted, femtosecond laser textured). Bioactivity in vitro tests are performed with human osteoblasts evaluating morphology, metabolic activity, and differentiation capabilities in direct contact with surfaces. Scanning electron microscope and profilometry analysis are used to evaluate surface morphology and samples' roughness with and without cells. All tested surfaces show good biocompatibility. The influence of material surface features is evident in the early evaluation, with the most promising results of morphological study for laser texturing. Deposition orientations seem to influence metabolic activities, with XZ orientation more effective than XY. Current data provide the first positive feedback on the biocompatibility of laser texturing finishing, still poorly described in the literature, and support the future clinical development of devices produced with a combination of LPBF and different finishing treatments.

Liquid vortex surface deformation probed by light reflection

Abstract We propose a method that combines an optical system with image processing techniques to scrutinize the dependence of liquid vortex deformation on varying angular velocity based on light reflection on liquid surfaces due to dynamic wettability. In our experiment, a broadened and collimated laser beam is directed onto curved surfaces, providing information on the vortex parameters through the analysis of the reflected beam profile. Additionally, the physical model of the liquid surface in a rotating cylinder before dewetting is examined. We investigate the liquid vortex forms of a saline solution and propylene glycol across various angular velocities, comparing them to the ideal parabolic vortex surface shape. The results show that, under the same experimental conditions, the vortex profiles of both solutions are equivalent. The vortex surface shape at certain angular velocity values, as determined by fitting plots, closely resembles a parabola, with R2 > 99%. The proposed method introduces a new approach for characterizing the dynamics of liquids as well as monitoring natural phenomena.

Study on evaluating and optimizing surface property mechanisms of polyalloy for enhanced OER catalyses via electrodeposited technique coupled with molecular dynamics simulations

Polyalloy catalysts composed of non-precious metals exhibit considerable potentials for facilitating overall water splitting processes in alkaline environments. However, achieving evaluations and optimizations of surface property mechanisms of polyalloy for enhanced OER catalyses is the key scientific issue that urgently need to be solved. Electrodeposited technique coupled with molecular dynamics simulations (MDS) are the key to breaking through aforementioned bottlenecks. Moreover, accurately predicting selections of non-precious metal elements in these catalysts through molecular dynamics simulations poses a challenging task. Here, three distinct non-precious metal catalysts (i.e. Ni, NiCo, and NiCoFe) were successfully synthesized through a facile one-step electrodeposition approach under a three-electrode system, which were deposited onto carbon sheets. For the sake of evaluating catalytic properties, MDS were utilized to calculate contact angles on ideal surfaces, which were verified by experimental measurements. Furthermore, ternary alloy catalysts (i.e. NiCoFe) exhibited an overpotential of 254 mV at a current density of 10 mA cm2 and exhibited remarkable stability over a prolonged 150-hour testing period. Finally, main findings can further underscore potentials of utilizing electrodeposition to fabricate efficient non-precious metal catalytic electrodes for overall water splitting, as well as the feasibility of indirectly predicting electrocatalytic performances of such cost-effective catalysts via MDS.

Targeting DLK1 in neuroblastoma

An ideal cell surface target has ubiquitously high cancer expression, absence from healthy tissues, and an essential role cancer initiation and/or maintenance. In this issue of Cancer Cell, Hamilton etal. combine proteomics, transcriptomics, epigenomics, and dependency databases to identify DLK1, a novel immunotherapeutic target for neuroblastoma.

Tectona Grandis seed powder and Mangifera Indica leaf powder as bio adsorbents for removal of methylene blue from aqueous solution

This study intends to describe the use of modified Tectona Grandis seed powder (TG) and Mangifera Indica leaf powder (MI) as adsorbent materials in the process of removing Methylene Blue (MB) from the wastewater. Nitric acid (HNO3) was used to chemically modify the plant material. Thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) were utilized to analyze the modified bio adsorbents (TG and MI). The adsorbents' ideal specific surface area and pore volume were measured using the N2 adsorption/desorption technique through detailed characterization. The efficiency of the adsorbents in eliminating the MB dye was investigated in relation to the effects of adsorbent dose (2–10 mg), pH (3, 7 and 10), exposure time (10–90 min), temperature (30 °C, 50 °C and 70 °C), and dye concentration (20–100 mg/L) through batch adsorption studies. The models proposed by Langmuir, Freundlich, and Dubinin Raduskevich (D-R) could explain the binding results for MB on TG and MI. The adsorption process were more in line with the Langmuir model than the other models. At neutral pH (7), TG showed maximum percentage removal of 73.12 % whereas, MI powder showed a maximum percentage removal of 50.5 %. Time and adsorbent dosage have both led to an increase in the percentage of methylene blue elimination. The experimental results were confirmed by density functional theory (DFT) calculations, which revealed energy gaps (ΔE) of 4.57 eV and 7.882 eV for MB and TG/MI, respectively. Following complexation, a change of 16.56 eV was observed, indicating a stronger MB adsorption tendency across the modified TG/MI surface. The primary components of the mechanism of MB adsorption include hydrogen bonds, n-π, π-π, and electrostatic interactions, as per the results of Monte Carlo(MC) simulation calculations. According to our findings, TG and MI are affordable biomaterials for treating wastewater in an ecologically responsible and green manner.

Defect-Free SiGe/Si Multistacks for Next-Generation CFET Architectures

The advancement of CMOS technology nodes demands increasingly complex device architectures. In this frame, the epitaxial deposition of complicated SiGe/Si multistacks presents a significant technology challenge required to enable such cutting-edge devices. This work presents the characterization of defect-free multistacks featuring SiGe layers with two possible Ge concentrations and incorporating either two or six stacked Si nanosheets. Our comprehensive analysis reveals good matching with the nominal structures and demonstrates remarkable repeatability of the Si channels. Furthermore, the multistacks exhibit ideal surface morphology, crucial for subsequent processing steps. Notably, the study also highlights optimal wafer-to-wafer repeatability, a key factor in ensuring consistent performance across large-scale production.

Novel Electrospun Zwitterionic Nanofibers for Point‐Of‐Care Nucleic Acid Isolation Strategies Under Mild Conditions

AbstractNucleic acid (NA) testing at the point‐of‐care requires efficient NA extraction followed by post‐NA amplification to achieve necessary detection sensitivity. Nanofibers (NFs) are demonstrated to be an ideal solid surface in an NA extraction process but necessitate harsh conditions that interfere with the subsequent NA amplification process. It is demonstrated that novel, pH tunable, zwitterionic NFs composed of uncharged nylon doped with the weakly basic, cationic polyallylamine hydrochloride and the weakly acidic anionic polycarboxylic acid to address the issue. Unlike the other cationic polymers investigated, e.g. polybrene and polyaniline, these polymers allow efficient NA extraction in Tris‐ethylenediamine tetra‐acetic acid buffer under mild conditions (pH 4.5 containing 0.1% Tween 20 for adsorption, and pH 10 with 50 mM NaCl for elution). Adsorption and elution yields over 95% and 70%, respectively, are achieved. It also discovered a correlation between material morphologies and the NA extraction suggests that the combination of polymer chemistries and nanofiber morphologies facilitates efficient NA extraction at low concentrations (ng range) within a short time period (<10 min). Considering the simple protocols and instrument‐free operation the as‐developed NFs are highly attractive for use in sample‐to‐answer NA testing in point‐of‐care settings.

One-Bath One-Step Dyeing of Polyester/Cotton Blend Fabrics Through Lipase Enzyme Surface Modification

ABSTRACT In this study, based on lipase enzyme surface modification, low-temperature dyeing of polyester/cotton fabric with reactive dyes was researched to achieve a one-bath one-step dyeing method. The Box-Behnken method was used to conduct the experiment and determine factors. For surface modification, lipase concentration, reaction time, and treatment temperature were considered, whereas for the dyeing process, reactive dye concentration, dyeing time, and dyeing temperature were taken into account to measure color strength. A treatment temperature of 30°C, a lipase enzyme concentration of 14 w/v %, and a treatment time of 50 min were shown to be ideal or optimum surface treatment parameters for lipase enzyme surface modification. The optimal or ideal conditions for the dyeing process were a reactive dye concentration of 2.13 w/v%, dyeing time of 50 min, and dyeing temperature of 66°C, giving a color strength value of 3.02. Lipase enzyme-modified polyester/cotton (p/c) blend fabrics provide color fastness that is all over grades 3–4, including washing fastness, rubbing fastness, perspiration fastness, and light fastness. The outcomes raise the prospect of developing a new technique for dying polyester/cotton blend fabric in a single bath using a single dye class that is typically employed for dying textiles made of cellulosic material.

Optical multiscale model for quantification of photon recycling including incoherent light scattering

A comprehensive multiscale modeling framework for photon recycling in textured solar cells and LEDs is introduced, treating light absorption and emission on an equal footing under consideration of the full internal mode spectrum in the device. The framework seamlessly merges a coherent emission model, free from unphysical divergence and ensuring consistency with detailed balance principles, with an incoherent net-radiation model applied to optically thick layers. The scattering of light at nonplanar interfaces is taken into consideration through four characteristic mappings that depict the microscopic scattering process, which are calculated analytically or by using a Monte-Carlo ray-tracing method. The final output of the model consists of energy- and angle-resolved local emission, re-absorption, and energy flux rates for further coupling to electronic transport. To validate the model, it has been compared with analytical solutions for the re-absorption probability in an ideal semiconductor slab with either ideal Lambertian or flat surfaces. The comparison between analytical and numerical calculations shows excellent agreement.

Tailoring MnO2 Cathode Interface via Organic–Inorganic Hybridization Engineering for Ultra‐Stable Aqueous Zinc‐Ion Batteries

AbstractManganese (Mn)‐based aqueous zinc ion batteries show great promise for large‐scale energy storage due to their high capacity, environmental friendliness, and low cost. However, they suffer from the severe capacity decay associated with the dissolution of Mn from the cathode/electrolyte interface. In this study, theoretical modeling inspires that the amino acid molecule, isoleucine (Ile), can be an ideal surface coating material for α‐MnO2 to stabilize the surface Mn lattice and mitigate Mn dissolution, thereby enhancing cycling stability. Furthermore, the coated Ile molecular layers can accumulate Zn2+ ions from the electrolyte and promote those ions’ transport to the α‐MnO2 cathode while prohibiting H2O from accessing the α‐MnO2 surface, reducing the surface erosion. The compact organic–inorganic interface is experimentally synthesized for α‐MnO2 utilizing Ile that shows homogeneous distribution on the well‐defined Ile‐α‐MnO2 nanorod electrodes. The fabricated aqueous zinc‐ion battery exhibits a high specific capacity (332.8 mAh g−1 at 0.1 A g−1) and excellent cycling stability (85% after 2000 cycles at 1 A g−1) as well as good inhibition toward Mn2+ dissolution, surpassing most reported cathode materials. This organic–inorganic hybrid interface design provides a new, simple avenue for developing high‐performance and low‐cost Mn‐based aqueous zinc ion batteries (AZIBs).

Integrated error modeling and evaluation of the effect for the Wolter-I grazing incidence focusing mirror imaging quality

The imaging quality determines the performance of the Wolter-I grazing incidence focusing mirror. When the design parameters are specified, the factors affecting the imaging quality are mainly machining and assembly errors; finding the error sources and evaluating the main errors are critical fundamental issues to achieve process optimization and control and improve imaging quality. This paper constructs a model of surface distribution error based on Nurbs surface reconstruction by non-contact measurement. It proposes an integrated error modeling method based on the surface distribution error model, taking into account the typical assembly errors such as decenter error, defocus error, and tilt error formed during the assembly process. First, the theoretical modeling and analysis of imaging quality based on ideal surface shape are carried out, and the sensitivity analysis of each error is carried out by using the Monte Carlo ray tracing method; then, an integrated optical simulation model containing assembly errors based on the surface distribution error is established, and the imaging quality of the focused spot is evaluated; finally, the experimental validation is carried out. The results of the experiment and the integrated optical simulation model show overall consistency, where the decenter error and defocus error have less influence, and the tilt error has the greatest impact, with the 10′ tilt error causing a decrease in the angular resolution of the focused spot of about 50″. The method can provide effective modeling and computational approaches to support the optimization of the precision assembly process and quality control of the Wolter-I grazing incidence focusing mirror.

Structures Associated with the Borromean Rings’ Complement in the Poincaré Ball

Guided by physical needs, we deal with the rotationally isotropic Poincar´e ball, when considering the complement of Borromean rings embedded in it. We consistently describe the geometry of the complement and realize the fundamental group as isometry subgroup in three dimensions. Applying this realization, we reveal normal stochastization and multifractal behavior within the examined model of directed random walks on the rooted Cayley tree, whose sixbranch graphs are associated with dendritic polymers. According to Penner, we construct the Teichm¨uller space of the decorated ideal octahedral surface related to the quotient space of the fundamental group action. Using the conformality of decoration, we define six moduli and the mapping class group generated by cyclic permutations of the ideal vertices. Intending to quantize the geometric area, we state the connection between the induced geometry and the sine-Gordon model. Due to such a correspondence we obtain the differential two-form in the cotangent bundle of the moduli space.

Synergistic adsorption and UV degradation of perfluorooctanoic acid by amine-functionalized A-center sphalerite

Perfluorinated alkylated substances (PFAS), as a category of persistent organic pollutants, have garnered extensive concern due to their resilience against environmental degradation. Herein, we developed an amine-functionalized sphalerite (ZnS) with adjustable surface amine functional groups and Zn defects (ZnS-X%[N]) by in situ coprecipitation and simple hydrothermal method in the presence of cetyltrimethylammonium bromide (CTAB). This material demonstrated efficient PFAS adsorption and subsequent photo-induced degradation under UV irradiation. The characterization results by TEM, BET, FTIR, XPS and EPR revealed that CTAB primarily influences ZnS by modulating surface amine functionalities, zinc defect density, and enhancing its photoreductive capacity. Adsorption and kinetic degradation experiments further showed that a medium CTAB concentration in ZnS-40%[N] achieves highest PFAS adsorption capacity (Cmax: 0.201 mol kg-1), and the corresponding decomposition rate was the fastest (kde: 1.53; kdf: 1.19). This efficacy is attributed to the ZnS-40%[N]'s ideal adsorptive sites and surface shallow defects. Moreover, theoretical simulation also supports the above experimental inference. Overall, ZnS-X%[N] exhibits a synergistic effect on PFAS adsorption and degradation, showcasing its potential for environmental adaptability and practical application.

The NACOB multi-surface walking dataset

Walking is a fundamental aspect of human movement, and understanding how irregular surfaces impact gait is crucial. Existing gait research often relies on laboratory settings with ideal surfaces, limiting the applicability of findings to real-world scenarios. While some irregular surface datasets exist, they are often small or lack biomechanical gait data. In this paper, we introduce a new irregular surface dataset with 134 participants walking on surfaces of varying irregularity, equipped with inertial measurement unit (IMU) sensors on the trunk and lower right limb (foot, shank, and thigh). Collected during the North American Congress on Biomechanics conference in 2022, the dataset aims to provide a valuable resource for studying biomechanical adaptations to irregular surfaces. We provide the detailed experimental protocol, as well as a technical validation in which we developed a machine learning model to predict the walking surface. The resulting model achieved an accuracy score of 95.8%, demonstrating the discriminating biomechanical characteristics of the dataset’s irregular surface gait data.

Zinc-cluster-based MOF with super selective separation adsorption catalyzes direct chemical conversion of CO2 from quasi-polluted air

The CO2 in the atmosphere has a serious impact on the human living environment. Direct utilization of the discharged CO2 to produce useful chemicals is challenging due to the practical presence of mixed compositions and low concentrations for CO2. Metal-organic frameworks (MOFs) with high internal surface areas, porosity and open metal site potentially possess superior adsorption separation capacity for CO2 gas, and on this basis, it can be used as a catalyst to achieve efficient chemical conversion of CO2. We demonstrate the synthesis and characterization of MOF ([Zn4O(ntba)2]n, MOF 1, H3ntba=4,4',4''-nitrilotribenzoic acid) with excellent selective adsorption properties from quasi- polluted air (O2, N2, CO2, CH4) and as heterogeneous catalysts for direct efficient CO2 chemical conversion. Detailed structural analysis reveals show that zinc ions behave multiple open metal sites and two coordination modes in the polyhedral zinc clusters building unit forming a 3D porous network by polyhedral zinc clusters building unit. Thanks to its structural features, at the 273 K and 100kPa, due to the ideal specific surface area and porosity, MOF 1 have ideal adsorption-separation performance for CO2, its values of adsorption and separation ratio are 75.7 cm3g−1, 373(CO2/N2), 79(CO2/O2), 13(CO2/CH4), respectively. The cycloaddition catalytic reaction investigations show that MOF 1 can be employed as a highly efficient catalyst for converting CO2 with a yield of 78 %. The result of this experiment offers the potential to directly utilize low-concentration and mixed CO2 while avoiding the economic and environmental costs of obtaining purified CO2 feeds tocks.

On metallic-type asteroid rotation moving in magnetic field (introducing magnetic second-grade YORP effect)

A novel physical effect has been found to be illuminated of being physically self-consistent to take into account in further calculating the dynamics of magnetically-induced asteroid rotation, surface of which has mostly a conducting structure (whereas such mostly metallic-formed asteroid is assumed to be moving in an external magnetic field of planet but in the meantime to be orbiting preferably outside its sphere of effective attraction). The last condition of asteroid surface's having conducting structure means the existence of the applied external torques stemming from the physical interactions between external magnetic field and iron asteroid itself (including long-term effect due to torques stemming from arising eddy-currents on metallic surface of asteroid). Such eddy-currents should heat the conducting surface of asteroid with further non-uniformly re-directing the heat flow from surface of asteroid into outer space via fluxes of thermal photons which carry momentum (in orthogonal direction to the surface which is in most cases far from the ideal surface of sphere). This leads by taking into account the overall outcome of heat flow (from the non-ideal surface of asteroid) to a kind of long-term magnetic second-grade YORP effect. System of Euler equations for aforementioned dynamics of asteroid magnetic rotation has been investigated in regard to existence of semi-analytical solution. Various perturbations (such as collisions, YORP effect) may destabilize the rotation of asteroid deviating it from the current spin state, whereas the electric(eddy)current-induced dissipation of energy reduces kinetic one of asteroid spin. So, dynamics of the asteroid rotation should result in a spinning about maximal-inertia axis with the proper spin state corresponding to minimal energy with a fixed angular momentum.

Effect of Er:YAG laser surface treatment on surface properties and shear-bond strength of resin-cement to three translucent zirconia: an in-vitro study

Achieving optimal bonding to translucent zirconia poses certain challenges as there is no consensus on ideal surface treatment method. Therefore, the aim of present investigation is to evaluate effect of Er:YAG laser treatment on wettability, surface roughness and shear-bond strength of three Yttrium oxide-stabilized zirconium-oxide polycrystal translucent zirconias. 120 specimens (n = 40/zirconia type) were prepared from three commercially-available translucent zirconias (Dentsply Sirona, USA); Translucent, High-Translucent and Ultra-Translucent zirconia. Specimens were sub-divided (n = 20/surface-treatment) into control and Er:YAG laser surface-treated groups. Regarding water wettability test; goniometer was used to measure contact angle before and after laser treatment. Surface roughness was measured using atomic force microscope. For shear-bond strength (SBS) test; the control group was sandblasted. All resin-bonded zirconia specimens were thermocycled for 5000 cycle and subjected to shear force using universal testing machine with 0.5 mm/min crosshead-speed. Results were statistically analyzed using two-way-analysis-of- variance (p ≤ 0.05). There was significant decrease in average contact angles (p-value < 0.001) in all zirconia groups after laser treatment. For roughness test; insignificant difference in average Ra in Translucent zirconia group. However, significant increase in mean Ra was revealed after laser treatment for High and Ultra-Translucent zirconias. For SBS test, results revealed insignificant difference in average bond strength after laser treatment in Translucent and High-translucent zirconia groups. Significant increase in mean bond strength of Ultra-Translucent zirconia was recorded. Er: YAG laser treatment significantly affected surface properties and shear-bond strength of Ultra-Translucent zirconia. Er:YAG laser irradiation may be a promising zirconia surface treatment method.Graphical

Effective post-cleaning strategy for vat photopolymerization 3D printed complex-structured polymer-derived ceramics

Achieving ideal surface quality and high structural integrity of complex-structured polymer-derived ceramics manufactured by vat photopolymerization 3D printing not only depends on the high temperature pyrolysis processing, but also greatly relies on the complete removal of uncured resin on the surface and internal holes of the green bodies before pyrolysis. In this work, based on the characteristics of precursor resins and printed bodies, a novel cleaning strategy with excellent cleaning efficiency and the corresponding cleaning agent with good flowability, appropriate solubility, and strong volatility are proposed. The cleaning agent consists of two monomers, butyl acrylate (BA), and 1,6-hexanediol diacrylate (HDDA), which are components of the precursor resin, thereby avoiding chemical reactions between the cleaning agent and the printed bodies. Meanwhile, by adjusting their proportions, suitable solubility and volatility are achieved. Appropriate solubility ensures that under diluted conditions, it does not damage the cured resin, while strong volatility removes the diluted uncured resin in a short time. Additionally, good flowability enables thorough penetration into the internal holes to mix with and contact the uncured resin. This cleaning process achieves efficient and high-quality cleaning of ceramic precursor 3D printed bodies. The superiority of this strategy is confirmed by observing and characterizing the pyrolyzed samples. It achieves high surface quality, structural fidelity, and no crack defects, with 100 % opening of 150μm diameter holes, high through-hole rates, low surface roughness, and peak-to-valley height. In contrast, traditional cleaning processes have lower through-hole rates, higher roughness, and suffer from structural distortion and cracks. Thus, the proposed post-cleaning strategy provides a guidance on the design and selection of cleaning agents and methods for effective cleaning of these ceramic samples derived from similar ceramic precursors.

Dysregulated T-cell homeostasis and decreased CD30+ Treg proliferating in aplastic anemia

Aplastic anemia (AA) is an autoimmune hematopoietic disease mediated by autoreactive T cells leading to bone marrow failure. However, the precise role of autoreactive T cells in the development of AA is not fully understood, hindering the advancement of therapeutic and diagnostic strategies. In this study, we conducted a single-cell transcriptome analysis of CD8+ T cells, conventional CD4+ T (CD4+ Tconv) cells, and Treg cells, to elucidate the potential disruption of T cell homeostasis in patients with AA. We identified changes in CD4+ Tconv cells, including loss of homeostasis in naïve and memory cells and increased differentiation potential in T helper type 1 (TH1), T helper type 2 (TH2), and T helper type 17 (TH17) cells. Additionally, we identified naïve and memory CD8+ T cells that were enforced into an effector state. CD127 is an ideal surface marker for assessing the immune state of CD8+ T cells，as identified by flow cytometry. Abnormal expression of TNFSF8 has been observed in AA and other autoimmune diseases. Flow cytometry analysis revealed that TNFRSF8 (CD30), a receptor for TNFSF8, was predominantly present in human Treg cells. Importantly, patients with AA have a decreased CD30+ Treg subset. RNA-sequencing analysis revealed, that the CD30+ Treg cells are characterized by high proliferation and a remarkable immunosuppressive phenotype. Taken, together, we propose that abnormal TNFSF8/TNFRSF8 signaling is involved in dysfunctional T cell immunity by increasing the destruction of CD30+ Treg cells.