Purpose: Sub-regions of hypoxia within the tumour microenvironment are associated with genetic instability and adverse prognosis after surgery or radiotherapy [1]. Identification of hypoxic tumours in patients prior to and during radiotherapy (RT) would facilitate stratification to hypoxia-modifying treatments, such as hypoxic cell radiosensitisers [2], hypoxic region RT dose escalation and avoidance of ultra-fractionated RT regimens. As validated hypoxia detection sequences on the MR Linac would allow the targeting and tracking of hypoxia during RT, we undertook sequence development for three candidate MR hypoxia detection biomarkers for use on the MR Linac.