BackgroundThis study aimed to characterize a tigecycline-resistant Hypervirulent Klebsiella pneumoniae (HvKP) strain, identified as KLZT, carrying a tigecycline resistance gene cluster tmexC2-tmexD2-toprJ2 belonging to ST29 and serotype K212. Materials and methodsAntimicrobial susceptibility and virulence phenotypes were assessed, followed by whole genome sequencing (WGS) using PacBio II and MiSeq sequencers. Genome annotation was conducted using the RAST server, and bioinformatics analysis revealed the genetic characteristics for this strain. ResultsAntimicrobial and virulence phenotype testing indicated that K.pneumoniae strain KLZT can be considered as the Multi-Drug Resistant HvKP. WGS analysis showed that it has one 5,536,506 bp chromosome containing three plasmids in the size of 290963bp (pKLZT-1), 199,302bp (pKLZT-2) and 4820bp (pKLZT-3). KLZT was classified as ST29 and K212 serotype. Five (blaSHV-187, oqxA, oqxB, fosA6) and 6 resistance genes (tmexC2-tmxeD2-toprJ2, blaOXA-1, aac(6′)-Ib-cr, catB3, arr-3 and blaLEN27) were identified from the chromosome and plasmid pKLZT-1, respectively. Resistance genes genetic context of plasmid pKLZT-1 analysis showed that tigecycline resistance gene cluster carrying region was flanked by umuC and umuD (umuD-hps-IS5-tmexC2-tmexD2-toprJ2-umuC) and other resistance genes and virulence factors (ureB, ureC and ureG) were carried by a (IS5075-Tn3-intI1 -aac(6′)-Ib-cr-blaOXA-1-catB3-arr-3-blaLEN27-Tn3-ISkpn26-ureBCG- IS5075). ConclusionsHere, we present the WGS of a Multi-Drug Resistant HvKP KLZT belonging to ST29 with capsular serotype K212 contains a muti-drug resistance plasmid.