Hyla Orientalis Research Articles

Ionizing radiation has negligible effects on the age, telomere length and corticosterone levels of Chornobyl tree frogs.

The accident that occurred at the Chornobyl nuclear power plant (Ukraine, 1986) contaminated a large extension of territory after the deposition of radioactive material. It is still under debate whether the chronic exposure to the radiation levels currently present in the area has long-term effects on organisms, such as decreases in longevity. Here, we investigate whether current levels of radiation in Chornobyl negatively impact the age of the Eastern tree frog Hyla orientalis. We also explore whether radiation induces changes in an ageing marker, telomere length or the stress hormone corticosterone. We found no effect of total individual absorbed radiation (including both external and internal exposure) on frog age (n = 197 individuals sampled in 3 consecutive years). We also did not find any relationship between individual absorbed radiation and telomere length, nor between individual absorbed radiation and corticosterone levels. Our results suggest that radiation levels currently experienced by Chornobyl tree frogs may not be high enough to cause severe chronic damage to semi-aquatic vertebrates such as this species. This is the first study addressing age and stress hormones in Chornobyl wildlife, and thus future research will confirm if these results can be extended to other taxa.

Not so cryptic-differences between mating calls of Hyla arborea and Hyla orientalis from Bulgaria.

Anurans are among the most vocally active vertebrate animals and emit calls with different functions. In order to attract a mate, during the breeding season male frogs produce mating calls which have species-specific structure and parameters, and have been successfully used to resolve issues in taxonomy and phylogenetic relations. This is particularly useful when closely related taxa are concerned, as many species are morphologically almost identical, but still their status is well-supported by molecular and genetic data, suggesting the existence of mechanisms for reproductive isolation. Such is the case for treefrogs from the Hyla arborea group, which are now recognized as several distinct species. The present study aims to establish differences in call parameters between the European tree frog, Hyla arborea, and the Eastern tree frog, Hyla orientalis, which both occur on the territory of Bulgaria. Using autonomous audio loggers, calls from six sites (three in the range of H. arborea and three in the range of H. orientalis) were recorded between 7 p.m. and 12 a.m. during the breeding season in 2020-2023. The following parameters in a total of 390 mating calls were analyzed: call count, pulse count, call series duration, call period, peak (dominant) frequency, entropy. Results indicated that sites formed two distinct groups, which corresponded to the known distribution ranges of H. arborea and H. orientalis. The first two components of the PCA explained 71% of the total variance, with variables call count, call series duration, peak frequency and entropy being most important for differentiation between the sites. This study presents the first attempt to differentiate between the calls of these two sister taxa, which both fall within the "short-call treefrogs" group, and results are discussed in terms of known data for mating calls in Hyla sp., as well as limitations and future perspectives.

ВПЛИВ ПОГОДНИХ УМОВ НА ЧИСЕЛЬНІСТЬ АМФІБІЙ ТА РЕПТИЛІЙ В УМОВАХ ПРИРОДНОГО ЗАПОВІДНИКА «МЕДОБОРИ» ЗА ПЕРІОД 2016–2020 РОКІВ

Based on the results of long-term observations, the influence of weather conditions on the dynamics of the number and biodiversity of amphibians and reptiles over five years in the "Medobory" nature reserve was analyzed. The number, reproduction, metamorphosis stages, and distribution of amphibians and reptiles depend significantly on climatic conditions. Low temperatures in spring inhibit spawning and larval development. During critical periods, a low amount of precipitation (e.g., 15.7 mm in May 2016, 9.1 mm in April 2018, and 12.1 mm in April 2019, compared to the norm of 45 mm) also negatively affects amphibian populations. This factor led to the disappearance of Triturus cristatus Laur., Lissotriton vulgaris L. (an outbreak was noted in 2014), and Bufo viridis Laur. from the reserve (last observed at MG-1 in 2013), as well as the less common Hyla orientalis Bedriaga. Amphibians and reptiles were recorded during the active period along two regular herpetological routes, MG-1 and MG-2, and observations were made during field research, covering all areas suitable for the reproduction and habitat of amphibians. Eleven species of amphibians have been confirmed in the reserve, belonging to six families: Salamandridae, Bombinatoridae, Pelobatidae, Bufonidae, Hylidae, and Ranidae. Among these amphibians, five species are listed in Appendix II of the Berne Convention, and one species (T. cristatus Laur.) is included in the Red Book of Ukraine. The most common amphibian species are Rana temporaria L., Rana arvalis Nilsson, and Bufo bufo L. Frogs such as Pelophylax lessonae Camerano and Pelophylax ridibundus Pallas occasionally occur within the reserve, as they require an aquatic environment even after reproduction. There are seven species of reptiles in the reserve and one in the immediate vicinity, out of the ten recorded in the Ternopil region. These reptiles occupy the most suitable habitats for their survival. The species represent five families: Emydidae (freshwater turtles), Anguidae (spindly lizards), Lacertidae (true lizards), Colubridae (snakes), and Viperidae (vipers). Common species include Lacerta agilis Andrz., Natrix natrix L., and Anguis fragilis L. Six species of reptiles are included in Appendix II of the Berne Convention, and the common copperhead (Coronella austriaca Laur.) is included in the Red Book of Ukraine.

Invasion of the North: The Eastern Tree Frog (<i>Hyla orientalis</i>) Range Shift in the East Baltic Sea Region

Peculiarities of distribution of eastern tree frog in Kaliningrad Oblast’ of Russia were studied. Until recently, only rare records of tree frogs were observed here, which are probably associated with introductions or penetration of the species from border regions of Lithuania and Poland. However, in the period 2007 – 2022 we revealed here 41 new localities of Hyla orientalis throughout the southern part of Kaliningrad Oblast’. In all studied localities, where we found mating tree frogs, shallow fish-free puddles surrounding by agricultural fields were used as breeding habitats. Local populations of the species are quite abundant. The northern boundary of native range of H. orientalis in 1940 was on the adjacent territory of north-eastern Poland. Thus, we recorded a shift to the north of the range boundary of the species by approximately 30 – 40 km over the past 82 years. This is most likely is related to global warming. In the region, the average temperature and the amount of precipitation are increasing, which lead to favorable conditions for the long-term existence of warm puddles in the fields, in which tadpoles can successfully and more rapidly develop. The appearance of a large number of such biotopes allowed this species to successfully populate this territory.

New data regarding the distribution and status of the herpetofauna from urban and peri-urban habitats in the city of Pitești, Argeș county (Romania)

Identifying urban and peri-urban areas inhabited by species of reptiles and amphibians represents the first step in understanding how herpetofauna can adapt to anthropogenic factors. This is particularly true for regions where sampling biases have left significant gaps in our knowledge of fauna distribution, such as Romania. The aim of the study is to show my preliminary observations on the herpetofauna and its use of habitats in the urban and peri-urban environments from the city of Pitești, Argeș County, located in southern Romania. I identified nine species of amphibians (Salamandra salamandra, Lissotriton vulgaris, Triturus cristatus, Bombina variegata, Bufo bufo, Bufotes viridis, Hyla orientalis, Pelophylax ridibundus and Rana dalmatina) and nine species of reptiles (Emys orbicularis, Trachemys scripta, Lacerta agilis, Lacerta viridis, Podarcis muralis, Anguis colchica, Coronella austriaca, Natrix natrix and Natrix tessellata). The most abundent species in urban environments were Podarcis muralis and Bufotes viridis, with other species naturally present in the same habitats being Lacerta viridis, Hyla orientalis and Bufo bufo. In an artificial urban pond there were present Emys orbicularis and the invasive species Trachemys scripta (together with other exotic turtles belonging to other genera) as a result of translocation and releases. When taking into account the peri-urban areas as well, the most abundant species in the study area were Bombina variegata, Rana dalmatina and Bufotes viridis, the first two being limited to the woodland area. The most widespread species were Hyla orientalis, Bufotes viridis, Lacerta viridis, Podarcis muralis and Natrix natrix. These widespread species were present throughout all the study area. I have also recorded some species of reptiles with very few observations in the Argeș County: Natrix tessellata and Trachemys scripta. Finally, I identified anthropogenic factors that negatively impact the herpetofauna, such as habitat loss, direct persecution, road mortality, invasive species and poaching.

PRELIMINARY OBSERVATIONS REGARDING THE HERPETOLOGICAL FAUNA FROM CORCOVA (MEHEDINȚI COUNTY)

The paper presents preliminary data on amphibian and reptile fauna from Corcova (Mehedinți county). The commune, with an area of 75.32 km2, is located in the south-west of Romania (north-east of the county), on the right bank of the Motru River. Hills prevail in terms of landforms. The study aimed to update the herpetological database with new information resulting from research conducted between March and October 2022. The preliminary list includes: 10 species of amphibians (Urodela - 3 species: Salamandra salamandra, Triturus cristatus, Lissotriton vulgaris and Anura - 7 species: Bombina bombina, Hyla orientalis, Pelobates fuscus, Bufotes viridis, Rana dalmatina, Pelophylax kl. esculentus, P. ridibundus) and 11 species of reptiles (2 species of turtles: Testudo hermanni, Emys orbicularis; 4 species of lizards: Lacerta agilis, L. viridis, Podarcis tauricus, Anguis fragilis; 5 snake species: Dolichophis caspius, Coronella austriaca, Zamenis longissimus, Natrix natrix, N. tessellata). The variety of habitats, the temperate-continental climate with sub-Mediterranean influences, the trophic resource have influenced the diversity of herpetofauna in the area. The highlighted species of amphibians and reptiles are listed on different national protectionist lists (ordinances, laws, etc.). Species that are subject to special habitat conservation measures have drawn our attention: Testudo hermanni, Emys orbicularis, Triturus cristatus, Bombina bombina, Pelobates fuscus (Annex 3 to OUG 57/2007).

Rice Fields as Important Habitats for Three Anuran Species-Significance and Implications for Conservation.

Agriculture can have different effects on wildlife depending on land-use type and extensive/intensive practices. The aim of this study was to establish the significance of rice fields in Central Bulgaria as anuran habitats. We used Audiomoth acoustic loggers to record calling activity at three sites near the city of Plovdiv-one shallow pond and two rice fields-from June to September in 2022 and 2023. We registered the three most widespread species in the region-Bufotes viridis, Hyla orientalis and Pelophylax ridibundus-and created pattern-matching models for them using the free web interface Arbimon, which enabled us to perform presence/absence counts and abundance estimates. At the beginning and the end of the rice vegetation period, water samples were collected and analysed for 62 substances. Substance concentrations were compared between ponds and with LC50 data from the available literature. We registered 19 and 21 substances in 2022 and 2023, respectively, with concentrations within the accepted limits, and although some metals were near risk levels, this was not reflected in the presence counts or the abundance estimates. The results indicated that frog activity was not related to any of the registered substances, but that it was positively correlated with daily rainfall and was higher in the rice fields.

Возрастная структура и рост восточной квакши (Hyla orientalis, Amphibia, Hylidae) в Самурском лесу (Северо-Восточный Кавказ)

The paper presents the results of studying the age structure and growth of the eastern tree frog (Hyla orientalis) in the Samur Forest. In the Primorskiy village (Magaramkent district, Republic of Dagestan, Russian Federation) in the first decade of May 2022, 7 females and 13 males were captured. The age of animals was determined by the cross sections of phalanx by standard method of skeletochronology. Age of females was 2–5 years (3.4 on average), males 1–6 years (3.4 on average). Life expectancy (ESP) in females is 4.33 years (S = 0.74), and in males 4.08 years (S = 0.72). The calculated maximum body length in females was 44.25 mm, males 41.54 mm. The growth rate of females is higher (k = 1.33) than that of males (k = 0.89).

Population transcriptogenomics highlights impaired metabolism and small population sizes in tree frogs living in the Chernobyl Exclusion Zone

BackgroundIndividual functional modifications shape the ability of wildlife populations to cope with anthropogenic environmental changes. But instead of adaptive response, human-altered environments can generate a succession of deleterious functional changes leading to the extinction of the population. To study how persistent anthropogenic changes impacted local species’ population status, we characterised population structure, genetic diversity and individual response of gene expression in the tree frog Hyla orientalis along a gradient of radioactive contamination around the Chernobyl nuclear power plant.ResultsWe detected lower effective population size in populations most exposed to ionizing radiation in the Chernobyl Exclusion Zone that is not compensated by migrations from surrounding areas. We also highlighted a decreased body condition of frogs living in the most contaminated area, a distinctive transcriptomics signature and stop-gained mutations in genes involved in energy metabolism. While the association with dose will remain correlational until further experiments, a body of evidence suggests the direct or indirect involvement of radiation exposure in these changes.ConclusionsDespite ongoing migration and lower total dose rates absorbed than at the time of the accident, our results demonstrate that Hyla orientalis specimens living in the Chernobyl Exclusion Zone are still undergoing deleterious changes, emphasizing the long-term impacts of the nuclear disaster.

Exposure to ionizing radiation and liver histopathology in the tree frogs of Chornobyl (Ukraine)

Ionizing radiation has the potential to damage organic molecules and decrease the health and survival of wildlife. The accident at the Chornobyl Nuclear Plant (Ukraine, 1986) led to the largest release of radioactive material to the environment. Among the different organs of a vertebrate, the liver plays a crucial role in detoxification processes, and has been used as a biomarker to investigate cellular damage in ecotoxicological research. Here, we examined the impact of the exposure to the current levels of ionizing radiation present in the Chornobyl Exclusion Zone on the liver of Eastern tree frogs (Hyla orientalis). We quantified the area of melanomacrophage cells and morphological variables of hepatocytes, two cell types often used to estimate damage caused by pollutants in vertebrates. First, we investigated whether these hepatic parameters were indicative of frog (individual) condition. Then, we analyzed the effect of individual absorbed dose rates and ambient radiation levels on frog livers. Most of the studied parameters were correlated with individual body condition (a good predictor of amphibian fitness and survival). We did not detect marked morphological lesions in the liver of frogs captured in medium-high radiation environments. The area occupied by melanomacrophages and the morphology of hepatocytes did not change across a gradient of radiocontamination covering two orders of magnitude. Once accounting for body condition and sampling locality, the area of melanomacrophages was lower in areas with high radiation levels. Finally, the area occupied by melanomacrophages was not linked to dorsal skin coloration. Our results indicate that current levels of radiation experienced by tree frogs in Chornobyl do not cause histopathological damage in their liver. These results agree with previous physiological work in the species in the Chornobyl area, and encourage further molecular and physiological research to fully disentangle the current impact of the Chornobyl accident on wildlife.

Ionizing radiation and melanism in Chornobyl tree frogs.

Human actions are altering ecosystems worldwide. Among human‐released pollutants, ionizing radiation arises as a rare but potentially devastating threat to natural systems. The Chornobyl accident (1986) represents the largest release of radioactive material to the environment. Our aim was to examine how exposure to radiation from the Chornobyl accident influences dorsal skin coloration of Eastern tree frog (Hyla orientalis) males sampled across a wide gradient of radioactive contamination in northern Ukraine. We assessed the relationship between skin frog coloration (which can act as a protective mechanism against ionizing radiation), radiation conditions and oxidative stress levels. Skin coloration was darker in localities closest to areas with high radiation levels at the time of the accident, whereas current radiation levels seemed not to influence skin coloration in Chornobyl tree frogs. Tree frogs living within the Chornobyl Exclusion Zone had a remarkably darker dorsal skin coloration than frogs from outside the Zone. The maintenance of dark skin coloration was not linked to physiological costs in terms of frog body condition or oxidative status, and we did not detect short‐term changes in frog coloration. Dark coloration is known to protect against different sources of radiation by neutralizing free radicals and reducing DNA damage, and, particularly melanin pigmentation has been proposed as a buffering mechanism against ionizing radiation. Our results suggest that exposure to high levels of ionizing radiation, likely at the time of the accident, may have been selected for darker coloration in Chornobyl tree frogs. Further studies are needed to determine the underlying mechanisms and evolutionary consequences of the patterns found here.

Assessment of exposure to ionizing radiation in Chernobyl tree frogs (Hyla orientalis)

Assessment of exposure to ionizing radiation in Chernobyl tree frogs (Hyla orientalis)

Strong Behavioral Effects of Omnivorous Fish on Amphibian Oviposition Habitat Selection: Potential Consequences for Ecosystem Shifts

Perceived predation risk to offspring may have similar ultimate community-level impacts to those of consumptive trophic interactions. The present study investigated the behavioral effects of common carpCyprinus carpio–an omnivorous fish capable of triggering an ecosystem shift to an algae-dominated state–on anurans, using a natural experiment conducted in a system of fish-stocked ponds. We compared oviposition patterns and larval densities of anurans and abundance of zooplankton and phytoplankton in ponds where fish were virtually absent and ponds where common carp was dominant. All studied anuran species bred in fish-poor ponds, while in ponds with high fish densities most of them oviposited infrequently or virtually did not breed. Oviposition habitat selection coupled with fish trophic pressure resulted in diametrically different tadpole densities between fish-poor and fish-dominated ponds. The alimentary tract contents of tadpoles of three locally common anurans,Pelobates fuscus,Hyla orientalis, andRana temporaria, contained large numbers of unicellular algae, but also significant numbers of zooplanktonic grazers. According to stable nitrogen isotope analyses, tadpoles occupied a trophic level similar to a primary consumer, snailLymnaea stagnalis, indicating that they fed mainly on algae. While total biomass of crustacean zooplankton did not differ between pond types, chlorophyllaconcentrations were low in fish-poor ponds compared to fish-dominated ponds and negatively related to total tadpole biomass. Our findings indicate that scarcity of anuran larvae, resulting mainly from the behavioral responses of breeding anurans to fish predation risk, may facilitate algal production, although ecosystem shifts in the presence of fish occur along more pathways than a top-down cascade.

Unusual evolution of tree frog populations in the Chernobyl exclusion zone.

Despite the ubiquity of pollutants in the environment, their long‐term ecological consequences are not always clear and still poorly studied. This is the case concerning the radioactive contamination of the environment following the major nuclear accident at the Chernobyl nuclear power plant. Notwithstanding the implications of evolutionary processes on the population status, few studies concern the evolution of organisms chronically exposed to ionizing radiation in the Chernobyl exclusion zone. Here, we examined genetic markers for 19 populations of Eastern tree frog (Hyla orientalis) sampled in the Chernobyl region about thirty years after the nuclear power plant accident to investigate microevolutionary processes ongoing in local populations. Genetic diversity estimated from nuclear and mitochondrial markers showed an absence of genetic erosion and higher mitochondrial diversity in tree frogs from the Chernobyl exclusion zone compared to other European populations. Moreover, the study of haplotype network permitted us to decipher the presence of an independent recent evolutionary history of Chernobyl exclusion zone's Eastern tree frogs caused by an elevated mutation rate compared to other European populations. By fitting to our data a model of haplotype network evolution, we suspected that Eastern tree frog populations in the Chernobyl exclusion zone have a high mitochondrial mutation rate and small effective population sizes. These data suggest that Eastern tree frog populations might offset the impact of deleterious mutations because of their large clutch size, but also question the long‐term impact of ionizing radiation on the status of other species living in the Chernobyl exclusion zone.

Prilog poznavanju batraho-i herpetofaune jugoistočne i južne Srbije

We present the updated distribution of batracho-and herpetofauna in southern and south-eastern Serbia, based on literature and new field records for 16 amphibian and 22 reptile species. In these two biogeographic regions, already recognized as biodiversity hotspots, we discovered two new amphibian species with limited distribution: Hyla orientalis and Pelobates balcanicus. Also, one species (Triturus ivanbureshi) has restricted distribution, while one (Bombina bombina) is rare. Four reptile species have limited distributions and six are rare in these regions. The presence of hybrid zones and the influence of the Mediterranean climate make southern and south-eastern Serbia areas of great importance and the target for future ecological and conservation studies in Serbia.

Distribucija gatalinki (Hyla SPP.) u Srbiji - implikacije skorašnje taksonomske revizije

We present the updated distribution data of the Hyla arborea species complex in Serbia. Hyla arborea inhabits all biogeographic regions, while Hyla orientalis inhabits only the easternmost parts of northeastern , Eastern and southeastern Serbia. Potential hybrid zone encompasses parts of central and southern Serbia from the Đerdap gorge, over Niš to Bosilegrad. Since the taxonomic separation UROŠEVIĆ, A. ET AL.: DISTRIBUTION OF TREE FROGS (HYLA SPP.) IN Serbia 138 between the tree frog taxa was not yet recognized in national nomenclature, we propose the official Serbian names. The species H. arborea will keep the standard Serbian name "Gatalinka", while for the species H. orientalis we propose the name "Istočna gatalinka".

Amphibian diversity of the Dnipro river within the limits of the Dnipro city agglomeration

Amphibians play an important role in the formation of biodiversity and functioning in most terrestrial and freshwater ecosystems. Urbanization threatens to survive many species due to the destruction of natural ecosystems whose remains become fragmented, isolated, and modified by urbanization processes. The floodplain and coastal ecosystems along both banks of the Dnipro River within the limits of the Dnipro city agglomeration are investigated. The current diversity of amphibians in this territory is represented by six species, which is 55% of the total amount of amphibian fauna of the Dnipropetrovsk region. They are Lissotriton vulgaris, Pelophylаx ridibundus, Pelobates vespertinus, Bombina bombina, Hyla orientalis, and Bufotes viridis. The largest diversity of amphibians was found on the site near the Samara Gulf, where small urbanization and anthropogenic influence are observed. The smallest diversity of amphibians was found for the site of residential community "Pobeda", where only two species of amphibians were revealed. The assessment of the similarity of amphibian communities shows that their diversity is the most similar for ecosystems of the right bank of the Dnipro River near the residential communities "Chervony Kamin", "Pokrovsky", and "Parus" and the ecosystems on the left bank near the Samara Gulf. The most numerous and widespread species is the marsh frog P. ridibundus, which lives along the coastline of the Dnieper River. Its populations are relatively stable according to their high number and distribution. The populations of L. vulgaris, B. bombina, and H. orientalis are under the threat of declining, as evidenced by their low number and dependence on small water bodies. Therefore, the reconstruction of the coastline and any earthwork and building activity should be accompanied by the measures on the preservation of small reservoirs along the Dnieper River. The obtained results develop our knowledge about the actual state of biodiversity and species wealth abundance of the Dnieper River within the northern steppe subzone of Ukraine and may be used for planning of the environmental protection measures.

Assessment of exposure to ionizing radiation in Chernobyl tree frogs (Hyla orientalis)

Ionizing radiation can damage organic molecules, causing detrimental effects on human and wildlife health. The accident at the Chernobyl nuclear power plant (1986) represents the largest release of radioactive material to the environment. An accurate estimation of the current exposure to radiation in wildlife, often reduced to ambient dose rate assessments, is crucial to understand the long-term impact of radiation on living organisms. Here, we present an evaluation of the sources and variation of current exposure to radiation in breeding Eastern tree frogs (Hyla orientalis) males living in the Chernobyl Exclusion Zone. Total absorbed dose rates in H. orientalis were highly variable, although generally below widely used thresholds considered harmful for animal health. Internal exposure was the main source of absorbed dose rate (81% on average), with 90Sr being the main contributor (78% of total dose rate, on average). These results highlight the importance of assessing both internal and external exposure levels in order to perform a robust evaluation of the exposure to radiation in wildlife. Further studies incorporating life-history, ecological, and evolutionary traits are needed to fully evaluate the effects that these exposure levels can have in amphibians and other taxa inhabiting radio-contaminated environments.

Lack of impact of radiation on blood physiology biomarkers of Chernobyl tree frogs

BackgroundHuman actions have altered natural ecosystems worldwide. Among the many pollutants released to the environment, ionizing radiation can cause severe damage at different molecular and functional levels. The accident in the Chernobyl Nuclear Power Plant (1986) caused the largest release of ionizing radiation to the environment in human history. Here, we examined the impact of the current exposure to ionizing radiation on blood physiology biomarkers of adult males of the Eastern tree frog (Hyla orientalis) inhabiting within and outside the Chernobyl Exclusion Zone. We measured the levels of eight blood parameters (sodium, potassium, chloride, ionized calcium, total carbon dioxide, glucose, urea nitrogen, and anion gap), physiological markers of homeostasis, as well as of liver and kidney function.ResultsLevels of blood physiology biomarkers did not vary in function of the current exposure of tree frogs to ionizing radiation within the Chernobyl Exclusion Zone. Physiological blood levels were similar in frogs inhabiting Chernobyl (both in areas with medium-high or low radiation) than in tree frogs living outside Chernobyl exposed only to background radiation levels.ConclusionsThe observed lack of effects of current radiation levels on blood biomarkers can be a consequence of the low levels of radiation currently experienced by Chernobyl tree frogs, but also to the fact that our sampling was restricted to active breeding males, i.e. potentially healthy adult individuals. Despite the clear absence of effects of current radiation levels on physiological blood parameters in tree frogs, more research covering different life stages and ecological scenarios is still needed to clarify the impact of ionizing radiation on the physiology, ecology, and dynamics of wildlife inhabiting radioactive-contaminated areas.

Faunistic review of amphibians in protected areas of Ukrainian Roztochia

Background. Study of amphibian fauna as a necessary component of ecosystems is always relevant and requires a regular renewal of information, especially in protected areas. We conducted a field investigation of amphibians, a literature review and an inventory of the collections of herpetological funds in relation to three protected areas: the Roztochia Biosphere Reserve, the Yavorivskyi National Park, and the Cholgini Ornithological Reserve. Materials and Methods. The field study was performed by classical methods: route method with manual catching and with herpetological nets, vocalization method, sampling of amphibians crushed on the road, and catching individuals with frog fences. We also analyzed the literature and amphibian collections in the Zoological Museum of Ivan Franko National University of Lviv. Results. According to the available literature, the amphibian fauna of these three areas under protection is similar and typical of this region. The results of our field research slightly differ from the literature data, thus, there is a need for further studies. On the study areas, we found 12 species of amphibians, which is more than half of the number of amphibian species in Ukraine, in particular: Smooth Newt Lissotriton vulgaris, Great Cres­ted Newt Triturus cristatus, Fire-bellied Toad Bombina bombina, Eastern Tree Frog Hyla orientalis, Common Spadefoot Toad Pelobates fuscus, Common Toad Bufo bufo, Green Toad Bufotes viridis, Common Frog Rana temporaria, Moor Frog Rana arvalis, Marsh Frog Pelophylax ridibundus, Edible Frog Pelophylax esculentus and Pool Frog Pelophylax lessonae. We detected 2 species of Caudata and 10 species of Anura. 10 species of amphibians were found in the Roztochia Biosphere Reserve, 5 species – in the Yavorivskyi National Park, and 9 species – in the Cholgini Ornithological Reserve. Conclusions. According to the field results, 4 species inhabit all three protected areas – Common Toad, Eastern Tree Frog, Marsh Frog and Edible Frog. A relatively small variety of amphibians found in the Yavorivskyi National Park can be due to the homogeneity of habitats and a proximity of one of the largest military proving grounds in Europe, which limits amphibians in choice of the reproductive and trophic habitats. These habitats deserve special attention because of their fast degradation for the last 10 years. Keywords: amphibians, fauna, protected areas, Ukrainian Roztochia, the Roztochia Biosphere Reserve, the Yavorivskyi National Park, the Cholgini Ornithological Reserve