Porous materials are able to exchange moisture with the surrounding air. The more hygroscopic they are, the more they contribute to regulate ambient humidity. This ability is characterized by the moisture buffer value (MBV) which is measured under dynamic solicitations according to different protocols. The NORDTEST protocol is the most commonly-used. It gives recommendations regarding the air velocity and the ambient conditions for initial stabilization. The purpose of this article is to measure the MBV according to the NORDTEST protocol and to study the effect of air velocity and of initial conditioning on the MBV results for different materials. Two mineral and two bio-based materials are considered: gypsum (GY), cellular concrete (CC), thermo-hemp (TH) and fine-hemp (FH). Following the NORDTEST classification, GY is a moderate hygric regulator, CC is good, TH and FH are excellent. When the air velocity ranges from 0.1 to 2.6 m/s, the MBV of GY and CC materials remains constant, but the MBV of TH and FH materials is highly affected. The initial conditioning has no effect on the MBV, but has an effect on the water content of the material, whatever the material.