Hydroxycarboxylic Acids Research Articles

Hydroxy-Carboxylic Acids as Green and Abundant Ligands for Sustainable Recovery of Copper from a Multimetallic Powder: A Proof of Concept.

The recovery of copper and other valuable metals had become increasingly strategic for the future of the global economy, particularly in regions lacking abundant mineral resources, such as most European countries. In this study, we investigated the viability of utilizing environmentally friendly, cost-effective, abundant and bio-based ligands, specifically carboxylic acids and their derivatives, for copper leaching in a low-temperature hydrometallurgical process. Our investigation focused on elucidating the impact of substituents in the α position of hydroxy-carboxylic acids on copper solubilization efficacy. Notably, hydroxy-carboxylic acids, like malic acid and lactic acid, were evidenced as particularly promising ligands for leaching copper from a custom-made multimetallic powder. By thoroughly characterizing the obtained complexes (Raman, UV-Vis) and by supporting the experimental efforts by a Design of Experiment (DoE) approach, we optimized the leaching process. The influence of experimental parameters such as pH, temperature, leaching time, and Cu/ligand molar ratio on process yield (determined through Inductively Coupled Plasma - Optical Emission Spectroscopy, ICP-OES, analysis) was thoroughly investigated. Additionally, we developed a subsequent copper recovery step by precipitating copper (II) hydroxide in an alkaline environment, guided by speciation diagrams tailored for each copper-ligand system.

Urine organic acid metabolomic profiling by gas chromatography mass spectrometry: Assessment of solvent extract evaporation parameters on the recovery of key diagnostic metabolites

BackgroundAnalysis of urinary organic acids (UOAs) by gas chromatography mass-spectrometry (GC–MS) is widely used in metabolomic studies. It is a complex test with many limitations and pitfalls yet there is limited evidence in the literature to support best practice. This study investigated the impact of drying down time and temperature on the recovery of 16 key analytes from solvent extracts. MethodsPooled urine specimens were enriched with organic acids. Urine aliquots (n = 3) were acidified and extracted into diethyl ether and ethyl acetate. Extracts were dried under nitrogen at ambient temperature (25 °C); 40 °C; 60 °C then left for 0; +5; +15 min. Dried extracts were derivatised with N,O,-bis-(trimethylsilyl)trifluoroacetamide prior to analysis by GC–MS. Urine specimens from individuals with biotinidase deficiency, maple syrup urine disease (MSUD) and ketotic hypoglycemia were analysed to demonstrate the potential clinical impact. ResultsRecovery of shorter chain hydroxycarboxylic acids decreased significantly when extracts were dried above 25 °C (mean recovery 89 % at 60 °C, p < 0.01) or left under nitrogen post-drying (mean recovery at ambient + 15 min, 40 °C + 15mins and 60 °C + 15mins was 56 %, 12 % and 2 %, respectively, p < 0.01). Whilst dicarboxylic acids/medium chain fatty acids were unaffected by temperature (mean recovery 100 %), prolonged drying reduced recovery (mean recovery 85 % at 60 °C + 15mins, p < 0.01). ConclusionsEvaporation of solvent extracts with heat and/or prolonged drying under nitrogen results in significant losses of the shorter chain hydroxycarboxylic acids. The evaporation protocol must be carefully controlled to ensure accurate and reproducible results, preventing misdiagnoses and/or misinterpretation of results.

New recyclable and functionalized chitosan-based polyurethane foams for effective and incessant removal of Orange II (OII) and Rhodamine B (RhB) dyes from water

The development of new efficient materials for the removal of water-soluble toxic organic dyes has been one of the focused research areas in the recent past. There is a strong demand for the new materials as most of the reported techniques/materials suffer from serious limitations. In this regard, a series of flexible chitosan-based task-specific polyurethane foams (PUCS-GP, PUCS-CA-GP, PUCS-TA-GP, and PUCS-GA-GP) associated with naturally available hydroxycarboxylic acids was developed. The basis for the preparation of these task-specific and functionalized PU foams is to possess amine groups for trapping the anionic dyes (example: Orange II denoted as OII) and carboxylic acid groups for attracting the cationic dyes (example: Rhodamine B denoted as RhB) under specified pH conditions. Batch adsorption experiments were conducted to assess and improve various parametric conditions. The experimental results revealed that the adsorption kinetics closely agree with the pseudo-second-order model having a maximum sorption capacity of 38.3 mg/g at pH 3 for OII on PUCS-GP and 48.4 mg/g at pH 6 for RhB on PUCS-CA-GP. Furthermore, the adsorption process was described by isotherms, kinetic equations and thermodynamic parameters (ΔG°, ΔH° and ΔS°). Notably, the regeneration of OII and RhB dyes from the exhausted PUCS-GP and PUCS-CA-GP materials was effectively accomplished. The recovered PUCS-GP shows >90 % OII and PUCS-CA-GP displays >70 % RhB removal efficiency even after twelve adsorption–desorption processes under mild conditions, demonstrating excellent recyclability/durability. The advantages of these functionalized foam materials are facile preparation, high adsorption capacity, good reusability, and very efficient removal of organic dyes from wastewater streams.

Novel ternary deep eutectic solvent fractionation for effective utilization of willow

A novel ternary deep eutectic solvent (TDES), consisting of zinc chloride, ethylene glycol and alpha hydroxy carboxylic acids (i.e., glycolic acid, citric acid and malic acid), was first proposed to effectively fractionate and convert willow (Salix matsudana cv. Zhuliu) into fermentable sugar. In particular, the zinc chloride/ethylene glycol/malic acid (ZnCl2/EG/MA) TDES system showed remarkable fractionation performance with 91.66 % xylan and 90.12 % lignin removals at 130 °C for 1.5 h, resulting in 96.01 % glucose yield in the subsequent enzymatic hydrolysis stage. Moreover, the regenerated lignin showed regular nanoparticle morphology and good antioxidant properties. Even after four recycling, the TDES showed 70.16 % of delignification and 83.70 % glucose yield with the TDES pretreated willow. Overall, this study demonstrated an effective solvent fractionation approach to maximize the utilization of total lignocellulose under mild conditions.

Thorium Complexation with Aliphatic and Aromatic Hydroxycarboxylates: A Combined Experimental and Theoretical Study.

Hydroxycarboxylic acids, viz., α-hydroxyisobutyric acid (HIBA) and mandelic acid (MA), have been widely employed as eluents for inner transition metal separation studies. Both extractants have identical functional groups (OH and COOH) with different side-chains. Despite their similarities in binding motifs, they show different retention behaviors for thorium and uranium in liquid chromatography. To understand the mechanism behind the trend, a detailed study on the aqueous phase interaction of thorium with both extractants is carried out by speciation, spectroscopy, and density functional theory-based calculations. Potentiometric titration experiments are carried out to reveal the stability and species formed. Electrospray ionization mass spectrometry is performed to identify the formation of different species by Th with both HIBA and MA. It is seen that for Th-HIBA and Th-MA, the dominating species are ML3 and ML4, respectively. A similar pattern observed in potentiometric speciation analysis supports the tendency of Th to form higher stoichiometric species with MA than with HIBA. The difference in the dominating species thus helps in explaining the reversal in the retention behavior of uranium and thorium in the reverse-phase liquid chromatographic separation. The results obtained are corroborated with extended X-ray absorption fine structure spectroscopic measurements and density functional theory (DFT) calculations.

Two Lactones Non-toxic to Humans Are Effective Repellents for the German Cockroach.

Lactones are cyclic esters of hydroxy carboxylic acids, present in several fruits and animals consumed by humans. There is evidence that some lactones modify insect behavior. The aim of the present study was to evaluate the repellent effect of four lactones (γ- and δ-nonalactone, and γ- and δ-dodecalactone) in first instar nymphs of the German cockroach, Blattella germanica (Linnaeus). To assess repellency, a nymph was placed on a circle of filter paper, half of which had been treated with lactone dissolved in acetone and the other half with acetone alone. The behavior of the nymph was recorded and the time the nymph spent in each half of the paper was quantified using Ethovision XT 10.1 software. Values of Distribution Coefficient (DC) were calculated: DC = (Tt - TA) / Tt, where Tt is the experimental time and TA is the time the nymph spent in the area treated with the repellent agent. DC can vary between 0 and 1. Values significantly higher than 0.5 indicate repellency. N,N-diethyl-meta-toluamide (DEET) was used as a positive control. DEET, δ- and γ-nonalactone caused repellency as from 77.9µg/cm2, whereas γ- and δ-dodecalactone had a repellent effect starting at 779.0µg/cm2. The values of DC for these concentrations were 0.89 (DEET), 0.86 (γ-nonalactone), 0.87 (δ-nonalactone), 0.83 (γ-dodecalactone), and 0.72 (δ-dodecalactone). To our knowledge, this is the first report of repellency produced by lactones in the German cockroach. This work allowed to identify two lactones that have a repellent effect similar to DEET.

Photocatalytic coproduction of hydrogen peroxide and Aldehydic/Keto acid on carbon nitride

Photocatalytic H2O2 production is emerging as an alternative technology to contribute H2O2-based advanced oxidation applications. However, the present reaction systems rarely consider the selective oxidation of sacrificial agent along with oxygen reduction reaction. Herein, we design a reaction system by using hydroxycarboxylic acids as additives to boost photocatalytic H2O2 production. More importantly, hydroxycarboxylic acids could simultaneously be dehydrogenated into aldehydic/keto acids with high selectivity. Lactic acid as one of the typical hydroxycarboxylic acids reveals the feasibility of this hypothesis, delivering 6.56 mM/h of H2O2 generation along with > 70 % pyruvic acid selectivity. The universality of this reaction system is demonstrated by using other hydroxycarboxylic acids. This works sheds new light on coupling H2O2 generation with value-added chemicals production by mild photocatalytic approach.

Biosynthesis of C4-C8 3-Hydroxycarboxylic Acids from Glucose through the Inverted Fatty Acid β-Oxidation by Metabolically Engineered Escherichia coli.

Inverted fatty acid β-oxidation represents a versatile biochemical platform for biosynthesis by the engineered microbial strains of numerous value-added chemicals from convenient and abundant renewable carbon sources, including biomass-derived sugars. Although, in recent years, significant progress has been made in the production through this pathway of n-alcohols, 1,3-diols, and carboxylic acids and its 2,3-unsaturated derivatives, the potential of the pathway for the biosynthesis of 3-hydroxycarboxylic acids remained almost undisclosed. In this study, we demonstrate the microaerobic production of even-chain-length C4-C8 3-hydroxycarboxylic acids from glucose through the inverted fatty acid β-oxidation by engineered E. coli strains. The notable accumulation of target compounds was achieved upon the strong constitutive expression of the genes atoB, fadA, fadB, fadE/fabI, and tesB, which code for the key enzymes catalysing reactions of aerobic fatty acid β-oxidation and thioesterase II, in strains devoid of mixed-acid fermentation pathways and lacking nonspecific thioesterase YciA. The best performing recombinants were able to synthesise up to 14.5 mM of 3-hydroxycarboxylic acids from glucose with a total yield of 0.34 mol/mol and a C4/C6/C8 ratio averaging approximately 63/28/9. The results provide a framework for the development of highly efficient strains and processes for the bio-based production of valuable 3-hydroxycarboxylates from renewable raw materials.

Modelling of reaction kinetics in production of hydroxy carboxylic acids by alkaline degradation of cellulosic waste

The reaction kinetics in alkaline degradation of cellulosic materials to monomeric compounds at elevated temperatures was studied. The target compounds are hydroxy carboxylic acids, preferably glucoisosaccharinic acid (GISA), that are valuable platform chemicals. This methodology, which focuses on depolymerizing cellulosic materials and transforming them into hydroxy carboxylic acids, has not been explored in existing literature. To this end, a rigorous mathematical model was developed that considers phenomena at macroscopic level (transformation of crystalline cellulose into amorphous) and at microscopic level (cleavage of glycosidic bonds). Experimental cellulose degradation data in 10 wt-% NaOH and 20–200 °C was correlated with the model. The agreement between the model results and the experimental data confirmed that the process obeys the proposed reaction pathway. Around 80 % of degradation occurs during the reactor warming up period. Analysis of rate constants indicates that GISA is not degraded into smaller hydroxy acids (SHA) at the temperatures studied. Instead, monosaccharides are converted into SHA as soon as they are produced. Conversion of crystalline cellulose into amorphous form was identified as the rate determining step.

Using Nuclear Magnetic Resonance to Troubleshoot a Stability Issue in a Real-World Formulation Chassis-Application to Consumer Oral Healthcare.

With direct application to current and future consumer healthcare products, this research sheds light on the importance of packaging and its potential effects on both Active Pharmaceutical Ingredient (API) delivery and stability. Industrially sourced, proprietary experimental formulations (PEFs), specifically oral cleansers, based on salicylic acid and hydrogen peroxide, discolored over time at different rates, depending on packaging type used. This discoloration stemmed from an interplay of two factors, involving both spontaneous formulation degradation and the interaction of both degradants and salicylic acid with the internal surface of the packaging. This manuscript reports on the investigation to uncover the origins of discoloration. To investigate this real-world, industrial pipeline problem, we exploited the high dimensionality and simple sample preparation uniquely afforded by NMR. Using a combination of 1D/2D NMR and diffusion-ordered NMR spectroscopy (DOSY) to leverage molecular mass estimations from, we not only quickly confirmed the identities of these degradants, but also assessed their formation as a function of temperature and pH, providing insight into the mechanisms underlying their formation. We were able to identify catechol as the main source of discoloration over a period of several weeks, being formed at the ppm level. Furthermore, we evaluated the formulation-container interaction, employing NMR, ICP-MS, and ATR-IR. Despite this comprehensive analysis, the root causes of discoloration could only tentatively be assigned to a surface Ti complex of salicylic acid and other hydroxy carboxylic acids. Through the understanding of formulation degradation pathways, we were able to support further toxicology assessment, vital to both consumer safety and the manufacturer. This work underscores the invaluable role of NMR in the analysis of intricate proprietary mixtures with a consumer-centric purpose. Our findings demonstrate that conventional analytical techniques falter in the face of such complexity, requiring extensive preparation and pre-analytical processing, highlighting the novelty and crucial relevance of NMR research to manufacturers and consumers. Such an analysis is of value in the pursuit of materials within the consumer-healthcare space, which meet the requirements for successful recycling or re-use.

New Biodegradable Copolymers Based on Betulin and Hydroxycarboxylic Acid Derivatives.

In this study, we propose an approach to the synthesis of new biodegradable polymer materials based on renewable raw feedstock (betulin) and derivatives of hydroxycarboxylic acids using a catalyst/catalytic system (γ-Al2O3, γ-Al2O3/TBHP) that is safe for health and the environment. The resulting polymers are linear thermoplastic polymers that undergo collapse upon melting in the presence of atmospheric oxygen. Moreover, these polymers demonstrate non-toxicity towards a range of Gram-positive and Gram-negative bacteria. The polycondensation of betulin with butyl lactate is particularly noteworthy.

Arriving the optimum retarder dosage level of sugar on the setting time, compressive strength, and microstructure property of Portland pozzolana cement

AbstractCement and concrete are the most crucial and dominating engineering materials in the construction sector. Cement serves as an excellent binder for concrete and when it is treated under unusual conditions either to satisfy accelerating or retarding requirements by the construction industries, admixtures role comes into the effect. The construction industry has been searching for retarding admixtures and the optimal dosage level, particularly for ready mix concrete applications. Generally the retarding admixtures such as organic retarders (lignosulphonates, hydroxycarboxylic acids and their salts, phosphonates, sugars) or inorganic retarders (phosphonates, borates) were generally used to reduce the speed of the reaction between cement and water by altering the growth of the hydration products and/or limiting the rate of water penetration to the cement particles. The research was still in its infancy stage in terms of determining not just the appropriate dosage amount, but also the ideal retarder materials for the aforementioned uses. In considering this industrial need, an attempt was made on utilizing the usage of sugar in the concrete to study the retarding effect without affecting the strength properties of cement. The sugars usage levels were at 5, 10, 15, and 20% of the usage of cement to investigate consistency, setting time, compressive strength and micro structure properties at the curing age of 7, 14 and 28 days on the mortar specimens. The conclusions of the study reported that setting time was extended with increase of the dosage levels of sugar. However in considering the strength property, less than 5% of sugar dosage level can be better for the application as retarding agent in the industry applications.

Biotransformation of 2-keto-4-hydroxybutyrate via aldol condensation using an efficient and thermostable carboligase from Deinococcus radiodurans

The bioconversion of 4-hydroxy-2-keto acid derivatives via aldol condensation of formaldehyde and pyruvate has received substantial attention as potential source of chemicals for production of amino acids, hydroxy carboxylic acids, and chiral aldehydes. We developed an environmentally friendly biocatalyst consisting of a novel thermostable class II pyruvate aldolase from Deinococcus radiodurans with maltose-binding protein (MBP-DrADL), which has specific activity of 46.3 µmol min–1 mg–1. Surprisingly, MBP-DrADL maintained over 60% of enzyme activity for 4 days at 50 to 65 °C, we used MBP-DrADL as the best candidate enzyme to produce 2-keto-4-hydroxybutyrate (2-KHB) from formaldehyde and pyruvate via aldol condensation. The optimum reaction conditions for 2-KHB production were 50 °C, pH 8.0, 5 mM Mg2+, 100 mM formaldehyde, and 200 mM pyruvate. Under these optimized conditions, MBP-DrADL produced 76.5 mM (8.94 g L–1) 2-KHB over 60 min with a volumetric productivity of 8.94 g L–1 h–1 and a specific productivity of 357.6 mg mg-enzyme–1 h–1. Furthermore, 2-KHB production was improved by continuous addition of substrates, which produced approximately 124.8 mM (14.6 g L–1) of 2-KHB over 60 min with a volumetric productivity and specific productivity of 14.6 g L–1 h–1 and 583.4 mg mg-enzyme–1 h–1, respectively. MBP-DrADL showed the highest specific productivity for 2-KHB production yet reported. Our study provides a highly efficient biocatalyst for the synthesis of 2-KHB and lays the foundation for large-scale production and application of high-value compounds from formaldehyde.Graphical

Protecting-group-free mechanosynthesis of amides from hydroxycarboxylic acids: application to the synthesis of imatinib

The mechanochemical protecting-group-free amidation of hydroxycarboxylic acids is presented. The transformation is applied to the synthesis of imatinib via a two-fold C–N bond construction sequence that bypasses a chlorinated genotoxic intermediate.

Electro-Oxidation of Diols in Polymer Recycling: Facilitating Separation and Refining Products

Increasing environmental awareness has highlighted the importance of plastic recycling and rightfully so, considering plastic pollution and an ever-increasing consumption of plastic products. Currently, numerous recycling strategies are being investigated with varying approaches depending on the type of polymer [1]. One attractive process is the chemical recycling of polyesters via alkaline hydrolysis which produces the dicarboxylic acid salts and diols of the respective polyester. The dicarboxylic acids can be separated by crystallization, while the diol separation remains a challenge. A process concept is proposed that utilizes two electrochemical separation technologies to process the polyester hydrolysis solution. A selective electro-oxidation of diols is proposed to mediate and simplify the challenging diol separation. The dicarboxylic acids can be crystalized using an electrochemical pH shift crystallization, which can simultaneously provide the base required for the alkaline hydrolysis of polyesters.The electrochemical pH shift crystallization utilizes water electrolysis in a membrane-separated parallel plate electrolyzer. The pH of the anode and cathode chambers is shifted towards acidic and basic pH values, respectively [2]. The acidification of the anolyte shifts the dissociation equilibrium of the respective dicarboxylic acid salt to the less water-soluble, fully protonated dicarboxylic acid. Crystallization is carried out within the electrolyzer, while the alkaline catholyte solution may be applied in the polyester hydrolysis.To circumvent the challenging removal of diols from aqueous solution [3], a selective electro-oxidation of said diols is proposed. The oxidation towards hydroxycarboxylic acids or dicarboxylic acids facilitates simplified separation processes such as pH shift extraction or crystallization, respectively. Rather than a closed-loop recycling towards both monomers, the proposed process suggests an upgrading of the diols to value-added molecules and crystallization of the dicarboxylic acid, which facilitates and simplifies separation.The study investigates the selective electro-oxidation of diols from three different polyesters: polyethylene terephthalate (PET), polytrimethylene terephthalate (PTT), and polybutylene succinate (PBS). A three-electrode setup utilizing plate electrodes as working electrodes is used in a custom cyclic voltammetry cell. Electro-oxidation is carried out in 1M KOH as electrolyte with diol concentrations ranging from 0.2 to 1M. Following the electrocatalyst activity studies, the selectivity of the electro-oxidation utilizing the most promising catalyst is studied in membrane-separated parallel plate electrolyzer experiments. A custom Luggin-Haber capillary setup monitors and controls the oxidation potential during the experiment. This presentation features the preliminary results of plate electrode catalysts’ electro-oxidation of three different diols in alkaline media. Selectivity studies for the oxidation of the three diols (ethylene glycol, 1,3-propane diol, and 1,4-butane diol) in the membrane-separated parallel plate electrolyzer are presented.[1] Hamad, K., Kaseem, M., & Deri, F. (2013). Recycling of waste from polymer materials: An overview of the recent works. Polymer Degradation and Stability, 98(12), 2801–2812. DOI: 10.1016/j.polymdegradstab.2013.09.025[2] Kocks, C., Wall, D., & Jupke, A. (2022). Evaluation of a Prototype for Electrochemical pH-Shift Crystallization of Succinic Acid. Materials (Basel, Switzerland), 15(23), 8412. DOI: 10.3390/ma15238412[3] Garcia-Chavez, L. Y., Hermans, A. J., Schuur, B., & Haan, A. B. de (2012). COSMO-RS assisted solvent screening for liquid–liquid extraction of mono ethylene glycol from aqueous streams. Separation and Purification Technology, 97, 2–10. DOI: 10.1016/j.seppur.2011.11.041

Influence of Carboxylic Acids on the Nucleation of Cementitious Phases Studied by Titrimetric Methods

AbstractIt is important to accurately control the setting of concrete and mortars. Functional admixtures such as hydroxy carboxylic acids can modify the hydration mechanism by influencing the dissolution of clinker phases and the crystallization of hydrate phases. While the crystallization of phases like CaCO3 under the influence of various additives has been studied intensively, there is substantially less understanding with regard to the main products of cement hydration, i.e., C‐S‐H, portlandite, ettringite, etc. In this work a potentiometry based setup was used to study the influence of succinic, tartaric, and citric acid on the nucleation and growth of portlandite and C‐S‐H. We found that the investigated molecules not only lead to a significant delay in nucleation by stabilization of prenucleation clusters, but also show a strong influence on the process of crystal growth. Further the effect of these molecules on the hydration reaction of tricalcium silicate was examined using ex‐situ calorimetry. We found a decent retarding effect on this reaction, even when there was almost no observable effect on the nucleation process in the previous experiments.

Orthosteric and allosteric modulation of human HCAR2 signaling complex

Hydroxycarboxylic acids are crucial metabolic intermediates involved in various physiological and pathological processes, some of which are recognized by specific hydroxycarboxylic acid receptors (HCARs). HCAR2 is one such receptor, activated by endogenous β-hydroxybutyrate (3-HB) and butyrate, and is the target for Niacin. Interest in HCAR2 has been driven by its potential as a therapeutic target in cardiovascular and neuroinflammatory diseases. However, the limited understanding of how ligands bind to this receptor has hindered the development of alternative drugs able to avoid the common flushing side-effects associated with Niacin therapy. Here, we present three high-resolution structures of HCAR2-Gi1 complexes bound to four different ligands, one potent synthetic agonist (MK-6892) bound alone, and the two structures bound to the allosteric agonist compound 9n in conjunction with either the endogenous ligand 3-HB or niacin. These structures coupled with our functional and computational analyses further our understanding of ligand recognition, allosteric modulation, and activation of HCAR2 and pave the way for the development of high-efficiency drugs with reduced side-effects.

ENANTIOSELECTIVE AND CHEMOSELECTIVE OPTICAL DETECTION OF CHIRAL ORGANIC COMPOUNDS WITHOUT RESORTING TO CHROMATOGRAPHY.

Enantiorecognition and resolution are of essential importance in many diverse areas of science. Whenever there arises a need to analyze/investigate enantiomers in different situations chromatography stands up in our minds immediately. Nevertheless, chemoselective and enantioselective recognition/discrimination (without going for separation) constitutes a different perception and requirement. The techniques using chiroptical sensing cause detection based on molecular interactions induced in different manners. Enantioselective sensing of monosaccharides in γ-cyclodextrin assembly and by diboronic acid based fluorescent sensors, application of bi-naphthol and H8BINOL based sensors and dendrimers, metal-to-ligand charge transfer transitions in CD, exciton-coupled circular dichroism, surface enhanced Raman spectroscopy, and enantioselective indicator displacement sensor arrays for enantioselective recognition/detection of chiral organic compounds, such as amines, amino acids/alcohols, and hydroxycarboxylic acids have been discussed in progressive manner with mechanistic explanations, wherever available. Besides, the chiroptical vs LC approach has been discussed. The present paper is focused on certain different non-chromatographic optical techniques and aims to extend an understanding and a view to consider such techniques which have been successful in selective detection, and determination of absolute configuration and enantiomeric excess, (without resorting to separation vis-à-vis LC) and that have potential use in high-throughput chiral assay and combinatorial search for asymmetric catalysts and reagents.

Metabolite-sensing GPCRs controlling interactions between adipose tissue and inflammation.

Metabolic disorders including obesity, diabetes and non-alcoholic steatohepatitis are a group of conditions characterised by chronic low-grade inflammation of metabolic tissues. There is now a growing appreciation that various metabolites released from adipose tissue serve as key signalling mediators, influencing this interaction with inflammation. G protein-coupled receptors (GPCRs) are the largest family of signal transduction proteins and most historically successful drug targets. The signalling pathways for several key adipose metabolites are mediated through GPCRs expressed both on the adipocytes themselves and on infiltrating macrophages. These include three main groups of GPCRs: the FFA4 receptor, which is activated by long chain free fatty acids; the HCA2 and HCA3 receptors, activated by hydroxy carboxylic acids; and the succinate receptor. Understanding the roles these metabolites and their receptors play in metabolic-immune interactions is critical to establishing how these GPCRs may be exploited for the treatment of metabolic disorders.

Hydroxycarboxylic acid receptors in GtoPdb v.2023.1

The hydroxycarboxylic acid family of receptors (ENSFM00500000271913, nomenclature as agreed by the NC-IUPHAR Subcommittee on Hydroxycarboxylic acid receptors [36, 12]) respond to organic acids, including the endogenous hydroxy carboxylic acids 3-hydroxy butyric acid and L-lactic acid, as well as the lipid lowering agents nicotinic acid (niacin), acipimox and acifran [53, 60, 65]. These receptors were provisionally described as nicotinic acid receptors, although nicotinic acid shows submicromolar potency at HCA2 receptors only and is unlikely to be the natural ligand [60, 65].