Hydrodynamic Surface Research Articles

Nanoparticles functionalised with recombinant single chain Fv antibody fragments (scFv) for the magnetic resonance imaging of cancer cells

Superparamagnetic iron oxide nanoparticles (SPIONs) can substantially improve the sensitivity of magnetic resonance imaging (MRI). We propose that SPIONs could be used to target and image cancer cells if functionalised with recombinant single chain Fv antibody fragments (scFv). We tested our hypothesis by generating antibody-functionalised (ab f) SPIONs using a scFv specific for carcinoembryonic antigen (CEA), an oncofoetal cell surface protein. SPIONs of different hydrodynamic diameter and surface chemistry were investigated and targeting was confirmed by ELISA, cellular iron uptake, confocal laser scanning microscopy (CLSM) and MRI. Results demonstrated that ab f-SPIONs bound specifically to CEA-expressing human tumour cells, generating selective image contrast on MRI. In addition, we observed that the cellular interaction of the ab f-SPIONs was influenced by hydrodynamic size and surface coating. The results indicate that ab f-SPIONs have potential for cancer-specific MRI.

Viscoelastic surface instabilities

We review three different types of viscoelastic surface instabilities: The Rayleigh -- Plateau, the Saffman -- Taylor and the Faraday instability. These instabilities are classical examples of hydrodynamic surface instabilities. The addition of a small amount of polymers to pure water can alter its flow behavior drastically and the type of instability may change not only quantitatively but also qualitatively. We will show that some of the observed new phenomena can be explained by the use of simple rheological models that contain most of the underlying physical mechanisms leading to the instability. A quantitative description however is often only possible close to the onset of the instability or for weak deviations from Newtonian behavior. A complete theoretical description is still lacking when the system is driven far from equilibrium or for fluids with strong non-Newtonian behavior.

Metal Deficiency Increases Aberrant Hydrophobicity of Mutant Superoxide Dismutases That Cause Amyotrophic Lateral Sclerosis

The mechanisms by which mutant variants of Cu/Zn-superoxide dismutase (SOD1) cause familial amyotrophic lateral sclerosis are not clearly understood. Evidence to date suggests that altered conformations of amyotrophic lateral sclerosis mutant SOD1s trigger perturbations of cellular homeostasis that ultimately cause motor neuron degeneration. In this study we correlated the metal contents and disulfide bond status of purified wild-type (WT) and mutant SOD1 proteins to changes in electrophoretic mobility and surface hydrophobicity as detected by 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence. As-isolated WT and mutant SOD1s were copper-deficient and exhibited mobilities that correlated with their expected negative charge. However, upon disulfide reduction and demetallation at physiological pH, both WT and mutant SOD1s underwent a conformational change that produced a slower mobility indicative of partial unfolding. Furthermore, although ANS did not bind appreciably to the WT holoenzyme, incubation of metal-deficient WT or mutant SOD1s with ANS increased the ANS fluorescence and shifted its peak toward shorter wavelengths. This increased interaction with ANS was greater for the mutant SOD1s and could be reversed by the addition of metal ions, especially Cu(2+), even for SOD1 variants incapable of forming the disulfide bond. Overall, our findings support the notion that misfolding associated with metal deficiency may facilitate aberrant interactions of SOD1 with itself or with other cellular constituents and may thereby contribute to neuronal toxicity.

"Scale oriented" embedded modeling of the North-Western Mediterranean in the frame of MFSTEP

Abstract. An embedded forecasting system was developed for the North-Western Mediterranean at 3-km resolution. The system is based on the Symphonie hydrodynamic free surface model and on the variational initialization and forcing platform VIFOP. The regional model is initialized and forced at its open lateral boundaries by the MFS GCM and forced at the surface by the ALADIN numerical weather prediction model. Once a week, a five-day forecast is produced after a hindcast of seven days. This pre-modeling period of 7 days before beginning the forecast allows the development of the small scale features associated to the high resolution. The relevance of the 5-day forecast strategy has been examined by comparing the forecasted fields to hindcast fields (forced by meteorological and oceanic analyses) considered as a reference. Mismatches remain at a very low level indicating a good quality of the forecasted forcing fields and also possibly to the strong wind conditions which prevailed during the period. The embedded forecasts have been compared to the MFS observing system (SST and MedArgo) during 6 forecast cycles between September 2004 and February 2005. It was basically found that in the North-Western Mediterranean, the MFS basin-scale model and thus the regional model forecasts are characterized by large negative biases of salinity in the first 100 m under the surface leading thus to too light subsurface waters. The underestimation of temperature by the regional model just below the surface and its overestimation at 30m deep can be attributed to an overestimation of the turbulent mixing. The regional model allows to represent a number of processes especially those induced by the wind as coastal upwelling under stratified conditions, dense water formation over the Gulf of Lion shelf, deep mixing in the convection zone or influence on the Northern Current penetration in the Gulf of Lion.

Fundamental problems with the model of uniform flow over acoustic linings

The walls of ducts containing flow are often acoustically lined in order to reduce sound. Many simple models of acoustic linings assume the lining to be linear and locally reacting; examples considered here include the three-parameter, mass–spring–damper, Helmholtz resonator and enhanced Helmholtz resonator models. All of these models have been found to have stability issues with uniform mean grazing flow, and there has been some confusion over the existence of hydrodynamic instability surface waves over such linings. Mathematically, the standard proven Briggs–Bers stability analysis is not applicable. Computationally, the hydrodynamic modes are routinely ignored (in the frequency domain) and instabilities filtered out (in the time domain). This confusion also causes significant problems for mode-matching, Green functions, and scattering analyses. In this paper, it is shown that any situation not capable of being analysed using the Briggs–Bers criterion is illposed, and that this is the root cause of the confusion over hydrodynamic instabilities. A large class of lining models, including all those mentioned above, are shown to be illposed with uniform mean flow. An explanation is given of the effects of this illposedness in practice, and it is argued that illposed models should not be used.An alternative stability criterion to Briggs–Bers has been occasionally used in the literature. This alternative criterion, involving analytic continuation to purely imaginary frequencies, was recently christened the “Crighton–Leppington” stability criterion. However, this stability criterion is incorrect, and several counter-examples are given here.

Microneedle mediated delivery of nanoparticles into human skin

The development of novel cutaneous delivery technologies that can produce micron-sized channels within the outermost skin layers has stimulated interest in the skin as an interface for localised and systemic delivery of macromolecular and nanoparticulate therapeutics. This investigation assesses the contribution of physicochemical factors to the rate and extent of nanoparticle delivery through microchannels created in a biological tissue, the skin, by novel delivery technologies such as the microneedle array. The hydrodynamic diameter, zeta potential and surface morphology of a representative fluorescent nanoparticle formulation were characterised. Permeation studies using static Franz-type diffusion cells assessed (i) the diffusion of nanoparticle formulations through a model membrane containing uniform cylindrical microchannels of variable diameter and (ii) nanoparticle penetration across microneedle treated human skin. Wet-etch microneedle array devices can be used to significantly enhance the intra/transdermal delivery of nanoparticle formulations. However the physicochemical factors, microchannel size and particle surface charge, have a significant influence on the permeation and subsequent distribution of a nanoparticle formulation within the skin. Further work is required to understand the behaviour of nanoparticle formulations within the biological environment and their interaction with the skin layers following disruption of the skin barrier with novel delivery devices such as the microneedle array.

Erosion of a granular bed driven by laminar fluid flow

Motivated by examples of erosive incision of channels in sand, we investigate the motion of individual grains in a granular bed driven by a laminar fluid to give us new insights into the relationship between hydrodynamic stress and surface granular flow. A closed cell of rectangular cross-section is partially filled with glass beads and a constant fluid fluxQflows through the cell. The refractive indices of the fluid and the glass beads are matched and the cell is illuminated with a laser sheet, allowing us to image individual beads. The bed erodes to a rest heighthrwhich depends onQ. The Shields threshold criterion assumes that the non-dimensional ratio θ of the viscous stress on the bed to the hydrostatic pressure difference across a grain is sufficient to predict the granular flux. Furthermore, the Shields criterion states that the granular flux is non-zero only for θ > θc. We find that the Shields criterion describes the observed relationshiphr∝Q1/2when the bed height is offset by approximately half a grain diameter. Introducing this offset in the estimation of θ yields a collapse of the measured Einstein numberq* to a power-law function of θ − θcwith exponent 1.75 ± 0.25. The dynamics of the bed height relaxation are described well by the power-law relationship between the granular flux and the bed stress.

Technology development for steering and stabilizers

As part of the All-Electric Ship concept the Ministry of Defence (MoD) Defence Procurement Agency Future Business Group's Marine Engineering Development Programme (MEDP) has been investigating the Electrical Actuation of Hydrodynamic Control Surfaces (ELAHCS) Programme for the Royal Navy. The project is presently examining the replacement of traditional hydraulic actuation systems for fixed-fin ship stabilizers, steering gear, and hydroplane actuation with novel electric actuation methods. As part of this programme the project has developed a technical demonstrator – a fixed-fin stabilizer actuator – which was fitted to both stabilizers on an operational Type 23 Frigate as part of a System Demonstrator Programme (SDP). This demonstrator is providing system performance and environmental data and contributing to the wider understanding of hydrodynamic control surface requirements. It is also illustrating the potential benefits of this technology which are reduced lifecycle costs, simpler installation and maintenance, improved survivability, cleaner systems, elimination of high-energy fluidic systems, and hence the associated support and training and hazards. This paper describes the development of the ELAHCS Programme, the progress of the SDP to date, and additional future exploitation of this technology for ship and submarine applications.

Hydrodynamic forces and surface topography: Centimeter‐scale spatial variation in wave forces

We measured the variability in hydrodynamic forces among locations separated by only centimeters along three horizontal transects on a steep rocky shore. Our results extend previous work showing that wave forces have the characteristics of 1/f‐noise, in which variability in wave forces decreases exponentially with decreasing scales of measurement. Furthermore, our results suggest that protection from hydrodynamic forces is not a certain consequence of a rugose substratum, suggesting that investigators should directly test (rather than assume) small‐scale topographic protection from hydrodynamic forces.

Micelles based on amphiphilic PCL‐PEO triblock and star‐shaped diblock copolymers: Potential in drug delivery applications

In this work, the potential in drug nanodelivery of micelles made from poly(epsilon-caprolactone) (PCL) and poly (ethyleneoxide) (PEO) copolymers with triblock and star-diblock architectures was explored. Linear and 4-arm star-shaped PCL macromers with two or four --OH end groups were prepared by ring-opening polymerization of CL and condensed with alpha-methoxy-omega-carboxy-PEO. The resulting amphiphilic copolymers were characterized by (1)H NMR, size exclusion chromatography, and differential scanning calorimetry. Separate PCL and PEO crystalline phases were observed for both copolymers. Copolymers self-assembled in water giving critical association concentrations in the range 0.010-0.023 mg/mL. Micelles with a size of 32-45 nm were prepared by dialysis and characterized for hydrodynamic diameter and surface charge. Their potential as nanocarriers in drug delivery applications was evaluated too. Micelles were nontoxic to both Red blood cells and HeLa cells. Complement activation experiments indicated that micelles can escape the reticuloendothelial system once intravenously injected. Finally, a different uptake on HeLa cells was found for micelles obtained from triblock and star-shaped copolymers.

Multifunctional Nanoparticulate Polyelectrolyte Complexes

Water-soluble, biodegradable, polymeric, polyelectrolyte complex dispersions (PECs) have evolved because of the limitations, in terms of toxicity, of the currently available systems. These aqueous nanoparticulate architectures offer a significant advantage for products that may be used as drug delivery systems in humans. PECs are created by mixing oppositely charged polyions. Their hydrodynamic diameter, surface charge, and polydispersity are highly dependent on concentration, ionic strength, pH, and molecular parameters of the polymers that are used. In particular, the complexation between polyelectrolytes with significantly different molecular weights leads to the formation of water-insoluble aggregates. Several PEC characteristics are favorable for cellular uptake and colloidal stability, including hydrodynamic diameter less than 200 nm, surface charge of >30 mV or <-30 mV, spherical morphology, and polydispersity index (PDI) indicative of a homogeneous distribution. Maintenance of these properties is critical for a successful delivery vehicle. This review focuses on the development and potential applications of PECs as multi-functional, site-specific nanoparticulate drug/gene delivery and imaging devices.

Friction Reduction in Mixed Lubrication

Minimization of frictional losses in the drivetrain of heavy-duty vehicles is important from both consumer satisfaction and environmental perspectives. Approaches to friction reduction in these components can be evaluated using simulation-based investigations. However, nearly all drivetrain components operate in the mixed lubrication regime which is difficult to model because both hydrodynamic lubrication and surface contact are significant and therefore, the total friction consists of hydrodynamic friction due to lubricant shearing and boundary film friction at asperity contact locations. Recent advances in simulation methods for mixed elastohydrodynamic lubrication (EHL) have enabled improved virtual design tools, such as those developed by Zhu and Hu and further improved by Liu et al. Here, these simulation tools are used to evaluate friction reduction and predict the effects on a mixed EHL interface under severe operating conditions. Three practical means of friction reduction are discussed based on the experimentally validated mixed lubrication friction model and its predictions made for representative, sample cases.

Review of factors affecting the release of water from cellulosic fibers during paper manufacture

The ease with which water is released from cellulosic fiber material during the manufacturing of paper can affect both the production rate and the consumption of energy during the manufacturing process. Important theoretical contributions to dewatering phenomena have been based on flow through packed beds of uniformly distributed fibers. Such descriptions are able to explain why resistance to dewatering increases as a function of the hydrodynamic surface area of fibers. More recent studies have demonstrated a critical role of finely divided matter. If the fines are unattached to fibers, then they tend to move freely through the fiber mat and plug channels in the paper web during the dewatering process. Dewatering also is affected by the deformability of cellulosic fibers and by whether the fibers easily slide past each other, thereby forming a dense mat. By emphasizing the role of fine matter, colloidal forces, and conformability of cellulosic materials, one can gain a more realistic understanding of strategies that papermakers use to enhance initial drainage and vacuum-induced dewatering.

On the regularity of the Neumann problem for free surfaces with surface tension

In 1952 H. Lewy established that a hydrodynamic free surface which is at least C 1 in a neighborhood of a point q situated on the free surface is automatically C, possibly in a smaller neighborhood of q. This local result is an example which preceeds the theory developed by D. Kinderlehrer, L. Nirenberg and J. Spruck (1977-79), proving that in many cases free surfaces cannot have an arbitrary regularity; in particular, there exist k, μ such that if the surface in question is C k,μ , then automatically is C ω . In this paper we extend their methods to Neumann type problems for free surfaces with surface tension.

Hydrodynamic surface fluctuations of polymer films by coherent X-ray scattering

We have applied X-ray photon correlation spectroscopy (XPCS) to measure the surface dynamics of polymer films of thicknesses down to a few times of the polymer radius of gyration. XPCS is currently the only technique to measure selectively dynamics of surface and/or interfacial fluctuations of the films thanks to high brilliance and coherence of the third generation synchrotron source. The results show the behavior of the capillary waves expected in viscous liquid when the film thickness is thicker than four times of the radius of gyration. However, thinner films show a deviation indicating the need to account for viscoelasticity. We present also the theory for surface dynamics of the thermally excited fluctuations on homogenous single-layer film with arbitrary depth is generalized to describe surface and interfacial dynamics of polymeric liquid bilayer films in terms of susceptibilities, power spectra and characteristic relaxation time constants. The effects on surface dynamics originating from viscosity inhomogeneities close to surface region are investigated by the bilayer theory and compared with the surface dynamics from homogeneous single-layer films under non-slip and slip boundary conditions.

Review of factors affecting the release of water from cellulosic fibers during paper manufacture

The ease with which water is released from cellulosic fiber material during the manufacturing of paper can affect both the production rate and the consumption of energy during the manufacturing process. Important theoretical contributions to dewatering phenomena have been based on flow through packed beds of uniformly distributed fibers. Such descriptions are able to explain why resistance to dewatering increases as a function of the hydrodynamic surface area of fibers. More recent studies have demonstrated a critical role of finely divided matter. If the fines are unattached to fibers, then they tend to move freely through the fiber mat and plug channels in the paper web during the dewatering process. Dewatering also is affected by the deformability of cellulosic fibers and by whether the fibers easily slide past each other, thereby forming a dense mat. By emphasizing the role of fine matter, colloidal forces, and conformability of cellulosic materials, one can gain a more realistic understanding of strategies that papermakers use to enhance initial drainage and vacuum-induced dewatering.

Hydrodynamic Dimensions, Electrophoretic Mobility, and Stability of Hydrophilic Quantum Dots

Luminescent semiconductor quantum dots (QDs) have great potential for use in biological assays and imaging. These nanocrystals are capped with surface ligands (bifunctional molecules, amphiphilic polymers, phospholipids, etc.) that render them hydrophilic and provide them with functional properties. These coatings alters their hydrodynamic radii and surface charge, which can drastically affect properties such as diffusion within the cell cytoplasm. Heavy atom techniques such as transmission electron microscopy and X-ray scattering probe the inorganic core and do not take into account the ligand coating. Herein we use dynamic light scattering to characterize the hydrodynamic radius (R(H)) of CdSe-ZnS QDs capped with various hydrophilic surface coatings (including dihydrolipoic acid and amphiphilic polymers) and self-assembled QD-protein bioconjugates. Experiments were complemented with measurements of the geometric size and zeta potential using agarose gel electrophoresis and laser Doppler velocimetry. We find that the effects of surface ligands on the hydrodynamic radius and on the nanoparticle mobility are complex and strongly depend on a combination of the inorganic core size and nature and lateral extension of the hydrophilic surface coating. These properties are critical for the design of QD-based biosensing assays as well as QD bioconjugate diffusion in live cells.

Electro-osmosis at inhomogeneous charged surfaces: Hydrodynamic versus electric friction

Electrokinetic methods are efficient in probing the electrostatic surface properties of charged systems. However, anomalies observed in experiments indicate that the classical electrokinetic theory should be reconsidered. Using Green's function methods and hydrodynamic simulations, we investigate electro-osmosis driven by electric-field-induced ion motion near a charged planar substrate with smooth or rough boundary. First, a reformulation of electro-osmotic theory for planar charged surfaces employing Green's functions shows that the Helmholtz-Smoluchowski (HS) relation between electrostatic potential and solvent velocity is exact for smooth surfaces, even in the presence of ion correlations. Deviations from HS theory are caused by combined hydrodynamic and electric surface friction, as our hydrodynamic simulations of ions at smooth and corrugated charged surfaces in lateral electric fields demonstrate. Within the simulations, hydrodynamic interactions are treated in the continuum limit and the presence of a no-slip boundary condition at the surface is taken into account. While electrofriction is relevant in highly charged system and/or for multivalent ions, hydrodynamic friction is dominant in systems with moderate surface charge density and/or low ionic valency. We also derive the effective electrokinetic surface charge from the electro-osmotic solvent profiles, which is substantially reduced when compared with the bare value and shows qualitative agreement with the experimental tendency.

Size, Charge, and Interactions with Giant Lipid Vesicles of Quantum Dots Coated with an Amphiphilic Macromolecule

Semiconductor colloidal quantum dots (QDs) are promising fluorescent probes for biology. Initially synthesized in organic solvents, they can be dispersed in aqueous solution by noncovalent coating with amphiphilic macromolecules, which renders the particles hydrophilic and modifies their interactions with other biological compounds. Here, after coating QDs with an alkyl-modified polyacrilic acid, we investigated their colloidal properties in aqueous buffers by electrophoresis, electron microscopy, light scattering, and rate zonal centrifugation. Despite polymer dispersity and variation of the size of the inorganic nanoparticles, the polymer-dot complexes appeared relatively well-defined in terms of hydrodynamic radius and surface charge. Our data show that these complexes contain isolated QD surrounded by a polymer layer with thickness 8-10 nm. We then analyzed their interaction with giant unilamellar vesicles, either neutral or cationic, by optical microscopy. At neutral pH, we found the absence of binding of the coated particles to lipid membrane, irrespective of their lipid composition. An abrupt surface aggregation of the nanoparticles on the lipid membrane was observed in a narrow pH range (6.0-6.2), indicative of critical binding triggered by polymer properties. The overall features of QDs coated with amphiphilic polymers open the route to using these nanoparticles in vivo as optically stable probes with switchable properties.

Experimental Detection and Reception Performance for Uplink Underwater Acoustic Communication Using a Remote, In-Air, Acousto-Optic Sensor

Covert communications between underwater and aerial platforms would increase the flexibility of surface and air vehicles engaged in undersea warfare by providing a new netcentric warfare communications capability and could have a variety of commercial and oceanographic applications. Research into an acousto-optic sensor shows promise as a means for detecting acoustic data projected toward the water surface from a submerged platform. The laser-based sensor probes the water surface to detect perturbations caused by an impinging acoustic pressure field. Experimental studies were conducted to demonstrate acousto-optic sensor feasibility for obtaining accurate phase preserved recordings of communication signals across the air-water interface. The recorded surface velocity signals were transferred to an acoustic communications receiver that used conventional acoustic telemetry algorithms such as adaptive equalization to decode the signal. The detected, equalized, and decoded bit error rate performance is presented for hydrostatic and more realistic, hydrodynamic water surface conditions