Hydroalcoholic Solution Research Articles

Zein decorated rifaximin nanosuspension: approach for sustained release and anti-bacterial efficacy enhancement.

Aim: The goal of the present work was to formulate zein-decorated rifaximin (RFX) nanosuspension to attain sustained release as well as effectiveness against Escherichia coli (E. coli).Methods: The RFX nanosuspension was fabricated by using antisolvent addition method followed by coating using hydroalcoholic zein solution. The optimized RFX-NS and RFX-NS@zein was lyophilized for further spectroscopic evaluations. In vitro antibacterial potential was elucidated using well diffusion method whereas MIC value was determined by microbroth dilution method against E. coli for RFX-NS and pure RFX.Results: Box-Behnken Design was employed to assess the effects of independent variables on quality target product profile of RFX-NS. Optimized RFX-NS depicted particle size of 193.5±4.45nm with 76.49±1.71% drug content. The significant change in particle size and zeta potential confirmed the formation of zein coated RFX-NS (RFX-NS@zein). In vitro release study depicted, 96.91±1.21% release of RFX from RFX-NS in 6h whereas 97.47±1.99% RFX release was observed from RFX-NS@zein at the end of 12h. Antibacterial assay of RFX-NS and free RFX against E. coli displayed MIC value of 15.44±0.01μg/ml and 72.96±0.25μg/ml, respectively.Conclusion: The results highlighted a significance of nanosuspension for improving the solubility of RFX and its antibacterial potential against E. coli.

Bio-Guided Extraction of a Phenolic-Rich Extract from Industrial Peanut Skin with Antioxidant and Hypotensive Potential.

Peanut composition includes phenolic compounds, especially in the skins, which are often not consumed. High blood pressure affects more than one billion people worldwide and is considered a high-risk factor for cardiovascular diseases. Several studies have correlated antihypertensive activity with the total phenolic content present in the plants. This study evaluated the hydroethanolic extraction of phenolic compounds from the industrial residue of peanut skin and evaluated the antioxidant and antihypertensive capacity of these extracts using in vitro models. A rotational central composite design (DCCR) was proposed to study the influence of the variables: (1) the ethanol concentration on the hydroalcoholic extractor solution, and (2) the proportion of solid sample (waste) per liquid in the extraction (mass/volume) in a simple solid-a liquid extraction process. The optimal extraction conditions within this model were 50% ethanol in water, and the proportion of sample to extraction solution (m/v) equaled to 0.2. The extract obtained had significant antioxidant capacity, both in chemical (ORAC) and in cellular models, with potential for free radical scavenging. Significant levels of ACE inhibition were also found, indicating antihypertensive activity.

Understanding of Wetting Mechanism Toward the Sticky Powder and Machine Learning in Predicting Granule Size Distribution Under High Shear Wet Granulation.

The granulation of traditional Chinese medicine (TCM) has attracted widespread attention, there is limited research on the high shear wet granulation (HSWG) and wetting mechanisms of sticky TCM powders, which profoundly impact the granule size distribution (GSD). Here we investigate the wetting mechanism of binders and the influence of various parameters on the GSD of HSWG and establish a GSD prediction model. Permeability and contact angle experiments combined with molecular dynamics (MD) simulations were used to explore the wetting mechanism of hydroalcoholic solutions with TCM powder. Machine learning (ML) was employed to build a GSD prediction model, feature importance explained the influence of features on the predictive performance of the model, and correlation analysis was used to assess the influence of various parameters on GSD. The results show that water increases powder viscosity, forming high-viscosity aggregates, while ethanol primarily acted as a wetting agent. The contact angle of water on the powder bed was the largest and decreased with an increase in ethanol concentration. Extreme Gradient Boosting (XGBoost) outperformed other models in overall prediction accuracy in GSD prediction, the binder had the greatest impact on the predictions and GSD, adjusting the amount and concentration of adhesive can control the adhesion and growth of granules while the impeller speed had the least influence on granulation. The study elucidates the wetting mechanism and provides a GSD prediction model, along with the impact of material properties, formulation, and process parameters obtained, aiding the intelligent manufacturing and formulation development of TMC.

2‐Oxazoline‐Based Polymer for Pharmaceutical Products Adsorption in Aqueous Media

ABSTRACTWe present the synthesis and comprehensive evaluation of a novel 2‐oxazoline‐based polymer (polyPhOx) functionalized with phenyl groups, designed for the efficient adsorption of pharmaceutical contaminants from aqueous solutions. Employing living polymerization techniques (CROP) with two distinct initiators, we optimize reaction parameters including time, temperature, and initiator concentration. The rigorous characterization of the obtained polymer is depicted as well as the demonstration of its significant binding capacity for various pharmaceuticals, exploiting π–π and electrostatic interactions to achieve superior adsorption efficiency. Adsorption experiments are performed using high concentrations of model pharmaceuticals, including aspirin, ibuprofen, metoclopramide, pyramidone, oestradiol, and indomethacin, in hydroalcoholic solutions. Initial investigations confirm polyPhOx's exceptional efficacy, particularly with hydrophobic compounds such as oestradiol, achieving adsorption capacities of up to 56.67 mg g−1. Further validation in real environmental samples highlights the polymer's practical applicability, showing substantial removal rates even under complex matrix conditions and bringing hope for effective water purification solutions. Remarkably, polyPhOx retains high adsorption performance after 10 cycles, underscoring its potential for sustainable and cost‐effective water purification. This study not only introduces a promising material for environmental remediation but also demonstrates its robustness and reusability, paving the way for advanced wastewater treatment solutions targeting persistent pharmaceutical pollutants.

Storage stability of phycobiliproteins in a hydroalcoholic solution evaluated by an optical method

The aim was to evaluate the stability of pigments of the phycobiliprotein group extracted from the biomass of the Spirulina (Arthrospira) platensis cyanobacterium and the Porphyridium purpureum red microalgae. Water extracts of phycobiliproteins were obtained following a double freezing of the raw biomass of Arthrospira platensis and Porphyridium purpureum. An extraction was carried out with a phosphate buffer (0.05 M, pH = 7) in the cold (5 °C) for 24 hours. To the extracts obtained, 96% ethanol was added until its concentration in the solution was 20%. The hydroalcoholic extracts of phycobiliproteins were stored for three months. Pigment concentrations were monitored by an optical method. The allophycocyanin pigment demonstrated the highest storage stability. The highest degradation rate of phycobiliproteins was observed during their storage in the light at room temperature. The degradation rate of pigments under these conditions was 9and 80-fold higher (for B-phycoerythrin and C-phycocyanin, respectively) than similar indices during their storage in the dark and in the cold. C-phycocyanin was the least stable, compared to other studied phycobiliproteins. Its degradation rate under all storage options was 5to 10-fold higher than that of B-phycoerythrin under similar conditions. An essential conservation requirement for C-phycocyanin and β-phycoerythrin in hydroalcoholic solutions was the absence of light. For C-phycocyanin, a low temperature was necessary as well. Storage of B-phycoerythrin in the dark at room temperature is acceptable. These conditions can ensure the conservation of up to 86% of pigments in hydroalcoholic solutions for 25–30 days.

Novel embelin-loaded transniosomes for topical delivery: comprehensive exploration of in vitro, ex vivo and dermatokinetic assessment for anti-cancer activity.

This study systematically designed and optimised a transniosomal formulation containing embelin for skin cancer management. The transniosomes were developed using a rotary evaporation method and then optimised using a Box-Behnken design. The optimized embelin-loaded transniosomes (Opt-EMB-TNs) exhibited a vesicle size of 149.01 nm, polydispersity index of 0.184, a zeta potential of -21.14 mV, an entrapment efficiency of 75.6 ± 0.65%, drug loading of 3.36 ± 0.03% and drug release of 80.88 ± 2.55%. The antioxidant potential of Opt-EMB-TNs was found to be 88.54% when compared to standard ascorbic acid. Dermatokinetic studies showed a greater drug deposition in targeted skin areas with Opt-EMB-TN gel compared to the embelin conventional gel (EMB-CF gel). In addition, the penetration depth study of the skin sample revealed that the transniosomal gel containing rhodamine B dye exhibited higher penetration than that of the rhodamine B dye containing hydroalcoholic solution. The efficacy of Opt-EMB-TNs for skin cancer was confirmed by cytotoxicity assay against the B16F10 melanoma cell line. The study concluded that the Opt-EMB-TN gel formulation is a promising and effective topical treatment for skin cancer, demonstrating significant potential for further development and clinical application. © 2024 Society of Chemical Industry.

Preparation of Polyphenol-Rich Herbal Beverages from White Willow (Salix alba) Bark with Potential Alzheimer’s Disease Inhibitory Activity In Silico

White willow (Salix alba) is a medicinal plant traditionally used to treat pain and inflammation. The aims of this study were to produce polyphenol-rich herbal beverages from willow bark with different ethanol content, temperatures, and solvent pH and to explore neuroprotective potentials of willow polyphenols. The phenolic compounds quantified in the willow infusions were salicin, chlorogenic acid, epicatechin, p-salicylic acid, and p-coumaric acid; the former three compounds exhibited promising inhibitory potentials against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) in molecular docking studies. Total phenol content and antioxidant activity were maximum when prepared with 50% ethanol-in-water at room temperature. Although aqueous infusions contained fewer total phenols than those extracted with 50% hydroalcoholic solutions, they enhanced the extraction of chlorogenic acid and salicin content, which may possess promising neuroprotective potentials. The addition of citric acids in hot water infusions led to a higher proportion of non-tannins and had a lighter appearance, which may result in less astringent mouthfeel and better consumer acceptance. Overall, the obtained results indicate that willow bark prepared with hot water and/or with addition of citric acids is rich in bioactive compounds with high antioxidant activity and possible neuroprotective activities in silico, which could serve as valuable ingredients for inclusion in functional beverages.

Game-based training to promote handwashing, handrub and gloving for hospital pharmacy operators

ObjectivesAdherence to handwashing, handrub and gloving procedures is mandatory for safe, aseptic drug compounding in hospital pharmacies. This study measured participants’ satisfaction and effectiveness of a game-based training tool (Handtastic...

Araucaria angustifolia and Picrasma crenata extracts as potential agents for the control of phytopathogenic fungi of agricultural interest

To expand knowledge about native species in southern Brazil, different types of extracts from the thin vinaceous bark of A. angustifolia and fragments of branches of P. crenata were evaluated. These were crushed and kept for fifteen days in contact with hydroalcoholic solutions at 70 % v/v and ethanolic (96 % v/v), seven days in the cold aqueous extract, and through the decoction process for 10 min. Afterward, they were evaluated at concentrations of 1.0 %, 2.5 %, 5.0 %, 10.0 %, and 20.0 % v/v on the mycelial development of the phytopathogenic fungi Colletotrichum gloeosporioides, Botrytis cinerea, and Sclerotinia sclerotiorum. The levels of phenolic compounds and total flavonoids were evaluated, and the alcoholic extracts of A. angustifolia had the highest total phenolic content. The main phenolic compounds identified in the P. crenata extract were resveratrol, followed by herperidin, rutin, ferulic acid, and kaempferol, and the main chemical compounds identified in the extracts of A. angustifolia were quercetin, rutin, ferulic acid, kaempferol, and gallic acid. A. angustifolia extracts and P. crenata, during the decoction, were more effective in controlling the phytopathogens C. gloeosporioides and B. cinerea from a concentration of 5 % v/v. The fungus S. sclerotiorum was moderately inhibited in hydroalcoholic extracts at 70 % v/v of P. crenata and A. angustifolia at 1.0 % and 2.5 % v/v concentrations. Effectively controlling these phytopathogenic fungi of agricultural importance confers importance on the conservation of these native species of socio-biodiversity in the Atlantic Forest in southern Brazil.

Kopexil vs minoxidil: In vivo comparative study on hair growth, hair growth promoting factors and toxicity

Introduction: This study aimed to investigate the impact of a hydro-alcoholic solution containing kopexil on hair growth, and growth factors, and to evaluate its skin penetration, as well as its potential toxic effects on the liver and kidneys in animal models. Methods: Animal studies were conducted on mice over 28 days, involving minoxidil (positive control), kopexil (test), and negative control groups. Morphological characteristics of skin and hair were assessed. Levels of hair growth-promoting markers (HGF and VEGF) were determined through western blot analysis. Toxic effects were examined by isolating and weighing the kidneys and livers, followed by histological examination. Results: The kopexil group demonstrated significant increases in hair weight, follicle count, percentage of anagen hair, and hair growth compared to the minoxidil. Western blot analysis revealed higher expression levels of hair growth-promoting factors in the kopexil-treated group. No statistically significant differences in liver and kidney weights or noticeable morphological variations were observed in the toxicity tests across the groups. Conclusion: The 5% (w/v) hydro-alcoholic solution containing kopexil proved to be an effective hair growth stimulator, influencing various factors. Its daily use can be considered a suitable treatment method for stimulating hair growth, given its improved effectiveness and ease of use for patients.

Potential effects of Resatorvid and alpha lipoic acid on gentamicin-induced nephrotoxicity in rats.

Gentamicin is an aminoglycoside antibiotic with a rapid bactericidal effect on the treatment of many infections. However, its use at high concentrations for more than 7 days causes nephrotoxic side effects. This study investigated the potential of Resatorvid and alpha lipoic acid (ALA) in mitigating gentamicin-induced nephrotoxicity in rats, considering biochemical, histopathological, and molecular parameters. This study randomly distributed 34 Wistar albino rats into four groups: healthy control (n = 6), Gentamicin (80 mg/kg, n = 7), Gentamicin + Sham (%10 hydroalcoholic solution, n = 7), Gentamicin + Resatorvid (5 mg/kg, n = 7), and Gentamicin + ALA (100 mg/kg, n = 7). Resatorvid treatment led to a statistically significant decrease in urinary IL-18, KIM-1, and NGAL levels, whereas ALA treatment significantly reduced KIM-1 levels compared to the gentamicin-only group. Both Resatorvid and ALA showed partial reductions in urine creatinine levels. Moreover, treatments with Resatorvid and ALA resulted in statistically significant decreases in NRF-2, CAS-3, and NR4A2 expressions. However, only Resatorvid demonstrated a statistically significant decrease in NF-B expression. These findings highlight the potential of Resatorvid in ameliorating gentamicin-induced nephrotoxicity, thereby expanding the therapeutic utility of gentamicin and enhancing its efficacy against infections.

Skin Tolerability of Oleic Acid Based Nanovesicles Designed for the Improvement of Icariin and Naproxen Percutaneous Permeation.

Deformable nanovesicles have a crucial role in topical drug delivery through the skin, due to their capability to pass intact the stratum corneum and epidermis (SCE) and significantly increase the efficacy and accumulation of payloads in the deeper layers of the skin. Namely, lipid-based ultradeformable nanovesicles are versatile and load bioactive molecules with different physicochemical properties. For this reason, this study aims to make oleic acid based nanovesicles (oleosomes) for the codelivery of icariin and sodium naproxen and increase their permeation through the skin. Oleosomes have suitable physicochemical properties and long-term stability for a potential dermal or transdermal application. The inclusion of oleic acid in the lipid bilayer increases 3-fold the deformable properties of oleosomes compared to conventional liposomes and significantly improves the percutaneous permeation of icariin and sodium naproxen through the human SCE membranes compared to hydroalcoholic solutions of both drugs. The tolerability studies on human volunteers demonstrate that oleosomes are safer and speed up the recovery of transepidermal water loss (TEWL) baselines compared to saline solution. These results highlight promising properties of icariin/sodium naproxen coloaded oleosomes for the treatment of skin disorders and suggest the potential future applications of these nanovesicles for further in vivo experiments.

Hybrid Organic–Inorganic Materials Prepared by Sol–Gel and Sol–Gel-Coating Method for Biomedical Use: Study and Synthetic Review of Synthesis and Properties

The need to improve the expectancy and quality of life of subjects affected by disabling pathologies that require the replacement or regeneration of tissues or parts of the body has fueled the development of innovative, better-performing materials that are capable of integrating into and being tolerated by body tissues. Materials with these characteristics, i.e., bio-functionality, bio-safety, and biocompatibility, are defined as biomaterials. One of the many methods for producing such materials is the sol–gel technique. This process is mainly used for the preparation of ceramic oxides at low temperatures, through hydrolysis and polycondensation reactions of organometallic compounds within a hydroalcoholic solution. This study is based on a specific type of biomaterial: organic–inorganic hybrids. The aim of this study is to provide an overview of the advantages and disadvantages of the sol–gel technique, as well as describe the preparation and chemical and biological characterization, uses, and future prospects of these biomaterials. In particular, the use of plant drugs as organic components of the hybrid material is the innovation of this manuscript. The biological properties of plant extracts are numerous, and for this reason, they deserve great attention from the scientific community.

Design, statistical optimization, and characterization of Esculin-loaded transliposome nanogel for topical delivery: In-vitro, ex vivo study and dermatokinetic evaluation

The purpose of this study was to prepare and optimise an Esculin-loaded transliposome (ECL-TL) for the dermal administration of ECL for skin cancer treatment. The ECL-TL formulation effectively produced closed lamellar vesicles with in-vitro drug release of 81.28 ± 2.82 % and ex-vivo penetration studies showed that had a 2.2-part intensified permeability compared to the conventional preparation. The CLSM data of skin visibly displayed intense permeation of ECL-TL formulated with rhodamine B, equating to the rhodamine B hydroalcoholic solution. The dermatokinetic study suggested that TLs enhance the permeability of the delivery system when topically applied. According to the data, the developed ECL-TL could be an effective drug nano-carrier for the dermal delivery of ECL in treating skin cancer.

Utilization of Antioxidant Fagraea fragrans fruit as Phytocosmetics

The objective is to test the antioxidant activity of methanol (MeOH) extracts, ethyl acetate (EtOAc) fractions, and an isolated compound of F. fragrans (tembesu) fruitby using DPPH method respectively. Antioxidant is one of the main components of phytocosmetic beside anti-inflammatory, anticancer, and antimicrobial activities while phytocosmetics of F. fragrans fruit is the cosmetics that mainly use extracts or components derived from this fruit only, without preservatives, such as products that do not contain water, oils, dry ointments, or hydro-alcoholic solutions. As a result, The IC50 values of the MeOH extracts, EtOAc fractions, and the isolated compound were 186.5 ± 0.52μg/mL, 357.2 ± 0.59 μg/mL, and 5.658 µg/mL respectively. The MeOH extracts exhibited moderate antioxidant activity while the isolated compound exhibited strong antioxidant activity. The total flavonoid content of the MeOH extracts and EtOAc fraction was measured by a colorimetric assay, using reagents of 1 M sodium acetic and 10% aluminum chloride. The EtOAc fraction had a total flavonoid content of 4.505 mgQE/g-extract and total phenolic content of 13.732 mgGAE/g-extract while the MeOH extracts had a total flavonoid content of 9.088 mg QE/g-extract and total phenolic content of 23.34 mgGAE/g-extract respectively. Therefore, the utilization of MeOH extracts or the isolated compound of these fruit may be beneficial for developing skincare phytocosmetics and personal care products

Formulation and Evaluation of Capsule-in-Capsule Technology for Biphasic delivery of Glipizide

In the present research work, to formulation and evaluation of capsule-in-capsule technology for biphasic delivery of Glipizide. The advantages of fast releasing liquid-filled-capsules and slow release beads-filled-capsules were combined to meet the optimized requirements of our biphasic drug delivery system. Glipizide slow releasing beads were prepared by ionotrophic gelation method by using natural polymers like Sodium alginate, Pectin and were filled into a smaller capsule. Glipizide fast releasing liquid dispersion was prepared by using either Castor oil carriers and further prepared a glipizide emulsion. This fast releasing liquid and slow releasing beads-filled-capsule was further inserted into a bigger capsule body and Seal the capsule by hydro alcoholic solution. The various formulation batches were subjected to physicochemical studies, entrapment efficiency, drug content, in vitro drug release and stability studies. Interaction studies reveal that there was no interaction between drug and polymers employed in this study. The optimized capsule-in-a-capsule formulation released 22.65±0.74% of drug at the end of 30 min and 95.04±0.88% of drug at the end of 12h. The drug release profile of Glipizide capsule-in-a-capsule formulation fits well with Pepas model followed by zero order, first order and Korsemeyer-peppa’s model. Korsmeyer-Peppas model analysis indicated that the drug release followed non-Fickian transport mechanism. The stability results indicate that the various parameters of our optimized formulation are not affected on storage at 45°C/75%RH up to 4 months. Target of capsule-in-capsule drug delivery loading dose reaches therapeutic drug level in blood plasma for quicker onset of action and Maintenance dose which maintain an effective therapeutic level for prolong period. The prepared Glipizide biphasic cap-in-cap will be used for treatment of Diabetes.

Formulation of Antioxidant Gummies Based on Gelatin Enriched with Citrus Fruit Peels Extract.

In this work, the peels of red and blonde oranges as well as lemons were efficiently (5.75-9.65% yield) extracted by hydroalcoholic solution with ultrasound assistance and employed as active molecule sources in the preparation of functional gummies. Antioxidant performances of the hydroalcoholic extracts were characterized by colorimetric assays, whereas LC-HRMS analyses identified the main bioactive compounds (phenolic acids and flavonoids). The highest scavenging activity was recorded for lemon extract in an aqueous environment (IC50 = 0.081 mg mL-1). An ecofriendly grafting procedure was performed to anchor polyphenols to gelatin chains, providing macromolecular systems characterized by thermal analysis and antioxidant properties. Scavenger abilities (IC50 = 0.201-0.454 mg mL-1) allowed the employment of the conjugates as functional ingredients in the preparation of gummies with remarkable antioxidant and rheological properties over time (14 days). These findings confirmed the possible employment of highly polluting wastes as valuable sources of bioactive compounds for functional gummies preparation.

The Potential of Cochlospermum tinctorium, Flueggea virosa, and Waltheria indica Traditional Plants From Burkina Faso in Treating Periodontitis: A Systematic Review.

Periodontitis is a chronic, infectious, and inflammatory oral disease with a high prevalence in developing countries, where limited access to modern dental care curtails its treatment. This review is dedicated to examining three indigenous botanical species frequently recommended by traditional therapists for the treatment of periodontal disease, namely, Cochlospermum tinctorium, Flueggea virosa, and Waltheria indica, with the aim of elucidating their chemical constituents and pharmacological properties that may support their empirical use. This review adheres to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines extension for scoping reviews. An electronic search was conducted in three databases (PubMed, Science Direct, and Google Scholar) up to July 2022. Out of 700 articles initially identified, only 11 were deemed eligible for inclusion; a substantial majority (80%) of these comprised in vitro studies. Among the trio of botanicals considered, Waltheria indica emerged as the most extensively investigated (65% of the studies). The administration of these plants was predominantly in the form of decoctions or macerations, with extraction methods employing alcoholic agents (ethanolic and methanolic), hydroalcoholic solutions, or aqueous solvents. The selected plants exhibited notable richness in polyphenolic compounds, particularly flavonoids, and demonstrated anti-inflammatory effects, as indicated in 60% of the studies, along with antibacterial properties (against Streptococcus aureus and Helicobacter pylori). None of the studies reported antibacterial activity against periodontal pathogens. The pharmacological properties of these plants may hold promise for the management of oral inflammatory and infectious conditions. Nevertheless, further comprehensive investigations are imperative to establish their safety and efficacy for periodontitis treatment before conclusive recommendations can be formulated.

Development and characterization of nintedanib inhalable powders as a potential pulmonary fibrosis treatment

Nintedanib is an orally administered intracellular tyrosine kinase inhibitor approved for idiopathic pulmonary fibrosis. Differently from oral treatment, dry powder inhalers (DPIs) can deliver a higher drug concentration at the target site, reducing doses and systemic side effects. The aim of this work was to develop dry powders of Nintedanib with properties suitable for inhalation therapy. Particles were prepared by spray drying the drug alone (3–5% w/v) or with appropriate excipients, from different hydroalcoholic solutions i.e., water/ethanol (7/3 v/v) or water/isopropyl alcohol (from 7/3 v/v to 5/5 v/v) mixtures. The powders obtained were characterized in terms of process yield, particle size distribution, morphology, density and aerodynamic properties. The results indicated that leucine improved the aerosol performance of the powders, increasing the value of the fine particle fraction (FPF) from 47.9 % in the drug only batches to 73.1 % using 5 % w/w leucine. This improvement was attributed to the ability of the amino acid to change both the surface area of the particles and the fluidity of the powder. Therefore, this study demonstrated the possibility of developing dry powder of Nintedanib for local administration in the lungs for the treatment of pulmonary fibrosis.

Fungitoxic effect and phytochemical characteristics of Brazilian Cerrado weeds against Rhizoctonia solani and Macrophomina phaseolina fungi.

The use of natural products obtained from plants, for example, invasive plants, offers a variety of allelochemicals with fungicidal potential. With this in perspective, the objective was to evaluate the fungicidal potential of ethanolic extracts of Cerrado plants on Rhizoctonia solani and Macrophomina phaseolina. The ethanolic hydroalcoholic extract of the 12 plants identified as invaders in the Brazilian Cerrado was prepared (Anacardium humile Saint Hill; Baccharis dracunculifolia DC.; Cenchrus echinatus L; Commelina erecta L.; Erigeron bonariensis L.; Digitaria horizontalis Willd.; Digitaria insularis L.; Porophyllum ruderale Jacq. Cass; Richardia brasiliensis Gomes; Sida rhombifolia L.; Turnera ulmifolia L.; Smilax fluminensis Steud)) and phytochemical screening and determination of total phenols and flavonoids were performed. To evaluate the in vitro antifungal activity, the hydroalcoholic solutions at concentrations of 800, 1200, 1600, 2000, and 2400 µL 100 mL-1 were separately incorporated into BDA agar and poured into Petri dishes, followed by the mycelium disk of the fungus. As a control, two solutions were prepared, one ethanolic solution added to the BDA medium (2400 µg 100 mL-1) and the other with BDA medium only. They were poured into Petri dishes, followed by a 0.5 cm diameter disk of mycelium of the fungus, incubated (23±2 ºC), with a 24-hour photoperiod. Among the constituents found in the plants, 75% are phenolic compounds, 58.3% are cardiotonic heterosides, 50% are steroids, 33.3% are flavonoids, 16.7% are anthraquinones, and 8.3% are alkaloids, saponins, and reducing sugars. Out of the 12 species, only the extracts of C. erecta and R. brasiliensis were active for M. phaseolina and R. solani. Thus, it is concluded that the ethanolic extract of C. erecta has the fungicidal potential to control diseases caused by fungi that are soil inhabitants. Of the other species, A. humille, B. dracuncufolia, D. insulares, C. erecta, D. insulares, P. ruderale, and R. brasiliensis have natural fungitoxic potential because they stand out in the content of polyphenols efficient in reducing the mycelial growth of M. phaseolina and R. solani.