Hydric Stress Research Articles

Digital Analysis with the Help of an Integrated UAV System for the Surveillance of Fruit and Wine Areas

The main purpose of this study was to create a prototype of an unmanned aerial system equipped with intelligent hardware and software technologies necessary for surveillance and monitoring the health and growth of crops from orchards with vines and fruit trees. Using low-cost sensors that accurately measure ultraviolet solar radiation was an important objective. The device, which needed to be attached to the commercial DJI Mini 4 Pro drone, had to be small, portable, and have low energy consumption. For this purpose, the widely used Vishay VEML6075 digital optical sensor was selected and implemented in a prototype, alongside a Raspberry Pi Zero 2 W minicomputer. To collect data from these sensors, a program written in Python was used, containing specific blocks for data acquisition from each sensor, to facilitate the monitoring of ultraviolet (UV) radiation, or battery current. By analyzing the data obtained from the sensors, several important conclusions were drawn that may provide valuable pathways for the further development of mobile or modular equipment. Furthermore, the plantation state analysis results with proposed models in the geographic information system (GIS) environment are also presented. The visualization of maps indicating variations in vegetation conditions led to identifying problems such as hydric stress.

Performance of bread wheat advanced lines under late sowing and hydric stress during the crop season 2020-2021

Thirty four advanced bread wheat lines and cultivar Borlaug 100 were sown on January 15 and 30, 2021, at the Norman E. Borlaug Experimental Station, in the Yaqui Valley, Sonora, México. Plots consisted of 1 bed 2 m long with two rows and 0.80 m apart with two replications, and a seed density of 100 kg ha-1 with two complementary irrigations. Average daily temperature (°C), maximum, minimum, relative humidity, rainfall, heat and cold units were recorded from January 1 to May 15, 2021. The average days for heading of the group was 69 for the first sowing date and 66 for the second one, while days for physiological maturity were 99 and 90, respectively. The average plant height of the group for the first date was 97.5 cm and 94 for the second one; the sister line SOKOLL/5/W15.92/4/PASTOR//HXL7573/2*BAU/3/ WBLL1/6/SOKOLL/3/PASTOR//HXL7573/2*BAU (PTSS12SHB00020T-0TOPB-099Y-099B-25Y-020Y-0B) consistently reached the maximum height with 107.5 and 110 cm in each date, respectively. The average grain yield per plot was 294 g; line BAJ #1*2/4/BETTY/3/CHEN/AE.SQ//2*OPATA showed the highest yield with 345.4 g which was above 4.3 t ha-1, followed by SOKOLL/3/PASTOR//HXL7573/2*BAU/4/ASTREB/5/PIHA//WORRAKATTA/2*PASTOR /3/PRL/2*PASTOR, also above 4.3 t ha-1. The average hourly temperature was 18.4 °C with a maximum of 40.8 °C and a minimum of 3.9 °C, and the number of heat and cold units was 203 and 393, respectively.

Exploring the relationship between field available water capacity (AWC) and atypical aging (ATA) development in base wines.

Hydric stress is a leading cause of atypical aging (ATA) in wine, characterized by unpleasant olfactory notes. The main sensorial and chemical marker of ATA is 2-aminoacetophenone (AAP). Early detection of ATA before the second fermentation in sparkling wines (SWs) is crucial for producing high-quality products. Climate change-induced droughts significantly impact agriculture, including grape farming, particularly in vineyards with shallow soils and reduced available water capacity (AWC). This study examined the relationship between AWC and ATA in base wines (BWs) intended for SW production. Ten vineyards were classified into three AWC categories (low, medium, and high). Hydric stress levels were monitored over two growing seasons to explore their effects on vegeto-productive behavior and AAP development. During the first vintage, drought conditions led to potentially ATA-tainted BWs across all AWC classes. The impact varied with AWC, with low-AWC vineyards experiencing higher stress and producing BWs with elevated AAP levels and vegeto-productive imbalance. In contrast, the following season had unusual rainfall, resulting in some potentially ATA-affected BWs, but no significant differences in AAP content or vegeto-productive balance among the AWC classes were observed. In conclusion, grapevines on low-AWC soils are at a higher risk of producing faulty BWs, particularly in dry vintages.

Geoenvironmental Effects of the Hydric Relationship Between the Del Sauce Wetland and the Laguna Verde Detritic Coastal Aquifer, Central Chile

In the central region of Chile, the Mega-Drought together with the demographic increase near the coast threatens groundwater availability and the hydrogeological functioning of coastal wetlands. To understand the hydric relationship between an aquifer and a wetland in a semi-arid coastal region of Central Chile (Valparaíso, Chile), as well as its geoenvironmental effects, four data collection campaigns were conducted in the wetland–estuary hydric system and surroundings, between 2021 and 2022, including physical, hydrochemical, and isotopic analyses in groundwater (n = 16 sites) and surface water (n = 8 sites). The results generated a conceptual model that indicates a hydraulic connection between the wetland and the aquifer, where the water use in one affects the availability in the other. With an average precipitation of 400 mm per year, the main recharge for both systems is rainwater. Three specific sources of pollution were identified from anthropic discharges that affect the water quality of the wetland and the estuary (flow from sanitary landfill, agricultural and livestock industry, and septic tank discharges in populated areas), exacerbated by the infiltration of seawater laterally and superficially through sandy sediments and the estuary, increasing salinity and electrical conductivity in the coastal zone (i.e., 3694 µS/cm). The Del Sauce subbasin faces strong hydric stress triggered by the poor conservation state of the riparian–coastal wetland and groundwater in the same area. This study provides a detailed understanding of hydrological interactions and serves as a model for understanding the possible effects on similar ecosystems, highlighting the need for integrated and appropriate environmental management.

Contrasted agronomical and physiological responses of five Coffea arabica genotypes under soil water deficit in field conditions.

Breeding programs have developed high-yielding Coffea arabica F1-hybrids as an adaptation against adverse conditions associated with climate change. However, theresponse to drought of coffee F1 hybrids has seldom been assessed. A trial was established with five C. arabica genotypes (2 pure lines: Catimor and Marsellesa and 3 F1 hybrids: Starmaya, Centroamericano and Mundo Maya) planted under the leguminous tree species Leuceana leucocephala. Coffee growth, yield and physiological responses were assessed under a rain-fed (control: CON) and a rainfall reduction treatment (RR) for 2 years. The RR treatment created a long-term rainfall deficit in a region with suboptimal temperature similar to those predicted by climate change scenarios. Moreover, the RR treatment reduced soil water content by 14% over 2 successive years of production and increased hydric stress of the three F1-hybrids (leaf water potentials averaged -0.8 MPa under RR compared with -0.4 MPa under CON). Under RR, coffee yields were reduced from 16 to 75% compared to CON. Mundo Maya F1 hybrid was the sole high-yielding genotype apable of sustaining its yield under RR conditions. Our results suggested that its significant increase in fine root density (CON = 300 and RR = 910 root.m-2) and its maintenance of photosynthetic rate (2.5 - 3.5 mmol CO2 m-2 s-1) at high evaporative demand might explain why this genotype maintained high yield under RR condition. This work highlights a possible drought tolerance mechanism in fruit bearing adult coffee trees where the plant fine root number increases to intake more water in order to preserve turgor and sustainphotosynthesis at high ETo and therefore conserves high yield in dry conditions.

How have atmospheric components of the local water cycle changed around the abrupt climatic shift over France?

The significant increase in surface air temperature experienced by Western Europe over the last few decades has resulted in an abrupt warming in France, around 1987/1988 years. This climatic shift impacted hydrological cycle, particularly by reducing runoff in spring and summer. Evapotranspiration and precipitation have been identified as the main drivers of climatic water balance. But the impact of the 1987/1988 climatic shift on local water cycle over France has not been quantified yet. This study tries to assess the consequences of this rapid warming on the main climatic components of local water cycle. Climate variables linked to the water cycle extracted from a reanalysed observed climate database are analysed using robust Bayesian change points detection and mean comparison techniques. After the abrupt rise in surface air temperature and surface solar radiation, water demand increases significantly on almost the entire French territory in spring, summer and autumn. Our results show that, from March to May, the vegetation cover is able to respond to this increase by drawing from the soil water reservoirs. But in summer, most of the territory is facing a significant rise in water constraint (i.e. difference between potential and actual evapotranspiration), extending on the last decade over autumn. In spring and summer, the increase in potential evapotranspiration is the main driver of the intensification of water constraint. In the beginning of autumn, longer dry spell also plays a major role in the lengthening of periods of water constraint. This innovative study highlights the specific impact of a climatic shift on the main components of the atmospheric water balance at regional and local scale. As the observed changes in climate hazard linked to water cycle affect growth cycle of the majority of the vegetation covers and crops, this could lead to a worsening of hydric stress events. As such climatic shifts are expected to happen again in the future, their impacts on the local water cycle represent a major issue for natural terrestrial ecosystems as well as for agriculture.

Ecological networks in savannas reflect different levels of hydric stress in adjacent palm swamp forest ecosystems.

Palm swamp forests are wetland ecosystems typical of the Brazilian Cerrado, which in recent decades have undergone intense changes due to land use alterations and climate change. As a result of these disturbances, many palm swamps have been experiencing significant drying, which can also affect adjacent vegetation. In the present study, we evaluated whether the drying of palm swamps affects the structure of plant-herbivore networks located in adjacent savanna areas in Brazil. Our results show that savanna areas adjacent to dry zones of palm swamps have fewer interactions, fewer interacting species, and a less specialized topology, which corroborates our expectations. Our findings indicate that the drying of palm swamps also has propagated impacts on adjacent savanna vegetation, impairing more specialized interactions in these environments. On the other hand, contrary to expectations, plant-herbivore networks in dry zones displayed higher modularity, lower nestedness and lower robustness than those in wet zones, suggesting that in dry environments, species tend to compartmentalize their interactions, even with lower interaction specialization. This is the first study to investigate the impacts of environmental drying on the structure of plant-herbivore networks in tropical ecosystems, highlighting the complexity of these effects and their differential impact on specialized and generalized interactions. Understanding these dynamics is crucial for developing effective conservation and management strategies in the face of ongoing environmental changes.

Enhancement of Forskolin Production Using Aeroponic Cultivation of Coleus forskohlii and the Impact on the Plant Phytochemistry.

Accessing plant resources to extract compounds of interest can sometimes be challenging. To facilitate access and limit the environmental impact, innovative cultivation strategies can be developed. Forskolin is a molecule of high interest, mainly found in the roots of Coleus forskohlii. The aim of this study was to develop aeroponic cultivation methods to provide a local source of Coleus forskohlii and to study the impact of abiotic stress on forskolin and bioactive metabolite production. Three cultivation itineraries (LED lighting, biostimulant, and hydric stress) along with a control itinerary were established. The forskolin content in the plant roots was quantified using HPLC-ELSD, and the results showed that LED treatment proved to be the most promising, increasing root biomass and the total forskolin content recovered at the end of the cultivation period threefold (710.1 ± 21.3 mg vs. 229.9 ± 17.7 mg). Statistical analysis comparing the LED itinerary to the control itinerary identified stress-affected metabolites, showing that LEDs positively influence mainly the concentration of phenolic compounds in the roots and diterpenes in the aerial parts of Coleus forskohlii. Moreover, to better define the phytochemical composition of Coleus forskohlii cultivated in France using aeroponic cultivation, an untargeted metabolomic analysis was conducted using UHPLC-HRMS/MS analysis and molecular networks on both the root and aerial parts. This study demonstrates that aeroponic cultivation, especially with the application of an LED treatment, could be a very promising alternative for a local source of Coleus forskohlii leading to easy access to the roots and aerial parts rich in forskolin and other bioactive compounds.

H2O2 priming: Biostimulation, drought tolerance and DNA methylation profile with intergenerational impact in tobacco plant

H2O2 is a promising priming agent due to its role in stress-response pathways and in the regulation of gene expression. Several studies have shown that H2O2 elicitation improves stress responses. However, information regarding of the intergenerational stress memory of the H2O2 priming is limited. Therefore, in this study, we evaluated the stress memory induced by H2O2 priming in tobacco plants by testing hydric stress phenotypic response and changes in DNA methylation in the parental priming line and in the next generation. Priming consisted of three foliar applications of 200 mM H2O2 every 5 days on 4-week-old plants. DNA methylation profiling was performed by Whole Genome Bisulphite Sequencing (WGBS). Hydric stress challenge consisted of three conditions: control (100 %), moderate (40–45 %) and severe (20–25 %) stress according to the available water field capacity. Plant response to the challenge was evaluated by morphological, biochemical, and molecular attributes. The results showed that H2O2 priming displayed a biostimulant effect on vegetative and root development, preventing growth stunting under drought conditions. Plant antioxidant activity was enhanced, as reflected by the decrease in endogenous H2O2. In drought conditions, proline content, CAT activity and gene expression of CHS, PIP1 and AQP1 genes were enhanced in the primed-plants (H), their progeny (F1), and the primed-progeny group (FH). DNA methylation profile revealed 795 differentially methylated cytosines (DmCs) in the progeny, with 89 associated with genes involved in cellular response to environmental stimuli. An interaction network highlighted stress response with flavoreductase enzyme as central interaction node, and in the second layer the Repressor of Silencing 1 (ROS1) gene. Thus, the findings suggest that H2O2 priming may induce intergenerational memory, presenting a potentially cost-effective strategy in stress management in agriculture.

Ecological stress memory in wood architecture of two Neotropical hickory species from central-eastern Mexico

BackgroundDrought periods are major evolutionary triggers of wood anatomical adaptive variation in Lower Tropical Montane Cloud Forests tree species. We tested the influence of historical drought events on the effects of ecological stress memory on latewood width and xylem vessel traits in two relict hickory species (Carya palmeri and Carya myristiciformis) from central-eastern Mexico. We hypothesized that latewood width would decrease during historical drought years, establishing correlations between growth and water stress conditions, and that moisture deficit during past tree growth between successive drought events, would impact on wood anatomical features. We analyzed latewood anatomical traits that developed during historical drought and pre- and post-drought years in both species.ResultsWe found that repeated periods of hydric stress left climatic signatures for annual latewood growth and xylem vessel traits that are essential for hydric adaptation in tropical montane hickory species.ConclusionsOur results demonstrate the existence of cause‒effect relationships in wood anatomical architecture and highlight the ecological stress memory linked with historical drought events. Thus, combined time-series analysis of latewood width and xylem vessel traits is a powerful tool for understanding the ecological behavior of hickory species.

The effect of successive summer drought periods on bacterial diversity along a plant species richness gradient.

Drought is a major stressor to soil microbial communities, and the intensification of climate change is predicted to increase hydric stress worldwide in the coming decades. As a possible mitigating factor for the consequences of prolonged drought periods, above and belowground biodiversity can increase ecosystem resistance and resilience by improving metabolic redundancy and complementarity as biodiversity increases. Here, we investigated the interaction effect between plant richness and successive, simulated summer drought on soil microbial communities during a period of 9 years.To do that, we made use of a well-established biodiversity experiment (The Jena Experiment) to investigate the response of microbial richness and community composition to successive drought periods alongside a plant richness gradient, which covers 1-, 2-, 4-, 8-, 16-, and 60-species plant communities. Plots were covered from natural precipitation by installing rain shelters 6 weeks every summer. Bulk soil samples were collected 1 year after the last summer drought was simulated. Our data indicate that bacterial richness increased after successive exposure to drought, with the increase being stable along the plant richness gradient. We identified a significant effect of plant species richness on the soil microbial community composition and determined the taxa significantly impacted by drought at each plant richness level. Our data successfully demonstrates that summer drought might have a legacy effect on soil bacterial communities.

Humidity modifies species-specific and age-dependent heat stress effects in an insect host-parasitoid interaction.

Climate change is projected to increase the frequency and intensity of extreme heat events, and may increase humidity levels, leading to coupled thermal and hydric stress. However, how humidity modulates the impacts of heat stress on species and their interactions is currently unknown. Using an insect host-parasitoid interaction: the Indian meal moth, Plodia interpunctella, and its endoparasitoid wasp, Venturia canescens, we investigated how humidity interacted with heat stress duration, applied at different host developmental stages, to affect life history traits. Hosts parasitized as 4th instar larvae and unparasitized hosts were maintained in high- (60.8% RH) or low-humidity (32.5% RH) at constant 28°C. They were then exposed to a 38°C thermal stress with a duration of 0 (no heat stress), 6 or 72 h in either the 4th or 5th host instar. Neither humidity nor heat stress duration affected emergence of unparasitized hosts, but increasing heat stress duration during the 4th instar decreased parasitoid emergence irrespective of humidity. When applied during the 5th instar, increasing heat duration decreased parasitoid emergence under low humidity, but no effect of heat stress was found under high humidity. Moreover, experiencing longer heat stress in the 4th instar increased host larval development time and decreased body size under high humidity, but this effect differed under low humidity; increasing heat duration in the 5th instar decreased parasitoid body sizes only under low humidity. Larval stage and heat stress duration directly affected parasitized host survival time, with a concomitant indirect reduction of parasitoid sizes. We show that humidity modifies key life history responses of hosts and parasitoids to heat stress in species-specific ways, highlighting the potential importance of humidity in regulating host-parasitoid interactions and their population dynamics. Finally, we emphasize that interactions between environmental stressors need to be considered in climate change research.

Are Bergmann's and Jordan's rules valid for a neotropical pitviper?

AbstractMorphological variation along the spatial distribution of species has been extensively investigated in ecological studies, and several ecogeographical rules explore the relationships between morphological traits and the environment. Many morphological traits are correlated, providing an opportunity to evaluate the validity of multiple ecogeographical rules simultaneously. Bergmann's rule predicts that endothermic animals in colder locations are larger than those in warmer locations. Jordan's rule predicts that fish from colder locations have more vertebrae than those from warmer locations. We tested the validity of Bergmann's and Jordan's rules for the neotropical lancehead snake Bothrops jararaca. We evaluated three morphological characters of 342 specimens: number of ventral scales (proxy for vertebrae number), snout–vent length (a linear measure of body size) and stoutness (volumetric body size). We implemented spatial regressions to evaluate the variation of morphological dimensions using climatic predictors: the minimum temperature and evapotranspiration. SVL was poorly related to minimum temperature and evapotranspiration. However, stouter individuals were found in colder places with greater evapotranspiration, following Bergmann's rule and the water conservation hypothesis. Individuals in warmer locations also had a greater number of ventral scales, reversing Jordan's rule. We showed that different selective pressures act on different morphological dimensions. Although stoutness follows Bergmann's rule, its variation would arise from an energy storage demand rather than heat conservation. Also, stoutness variation along evapotranspiration gradients could represent a mechanism to avoid hydric stress in environments with considerable climatic variations. The variation in vertebrae number along temperature gradients could be related to ecological factors and foraging. We highlight that physioecological mechanisms to deal with climatic variation and ecological aspects could be identified in snakes through intraspecific analyses, contrasting with interspecific studies that can hardly detect general trends. Due to different environmental effects on body size, we shed new light on the importance of exploring multiple morphological dimensions in macroecological studies.

Methodological approaches in the study of hydric stress in plants

Drought significantly affects plant growth and development, having a negative impact on the productivity of crops and, respectively, on food security worldwide. To predict the response of agricultural crops to future global warming and water deficit and to develop effective risk management strategies, there is a need to understand, at various levels of organization, the processes that determine tolerance to adverse environments. In this context it is important to use appropriate models of hydric stress induction and response assessment. The paper presents a brief description of the methodological approaches used in plant drought stress studies (field, greenhouse, laboratory experiments; experimental models using different types of substrates; distinct methods to induce water deficit), reflects their particularities, advantages and limitations and highlights the importance of combining and adapting methods of analysis according to the studied species, the environment and the research objectives.

Assessing groundwater quality in semi-arid conditions: a geographic information systems-integrated approach using water quality index

Meeting freshwater demands in water-scarce regions is imperative, given challenges like hydric stress, urbanization, aridity, and population growth. This study focuses on the Tebessa aquifer system, a critical water source facing escalating demands. A primary objective is to identify actionable management strategies to improve groundwater quality dynamics in semi-arid regions. The methodology involves a comprehensive assessment of groundwater health using the Water Quality Index (WQI) approach. This method integrates diverse parameters influenced by intensive agricultural and industrial activities. Additionally, spatial relationship analysis, facilitated by Geographic Information System (GIS) technology, is employed to gain insights into complex hydrogeological processes. The multi-parameter strategy implemented results in water quality maps that highlight various ions, revealing spatial disparities and assigning quality priority classes from 'Good' to 'Poor' across the groundwater. The research, conducted in the semi-arid Tebessa-Morsott Plain, encompasses field and laboratory investigations to delineate hydrochemical traits, including alkalinity, salinity, and heightened ion concentrations. The results of the study contribute to scientific understanding by uncovering interrelationships and spatial intricacies, offering valuable insights for water resource governance. The effectiveness of the methodology, incorporating WQI and GIS, is demonstrated in the comprehensive evaluation of groundwater quality. Ultimately, this research provides a foundation for informed decision-making and sustainable management of groundwater resources in semi-arid regions.

High levels of genetic variation and differentiation in wild tropical gourds provide a novel resource for cucurbit crop improvement

Social Impact StatementThe genetic variation of crop wild relatives will be key for our survival, as environmental change represents an increasing global threat for agriculture and food security. Cucurbita lundelliana and Cucurbita okeechobeensis subsp. martinezii are wild relatives of cultivated squashes and pumpkins that could be used for crop improvement. A genomic approach was used to characterize genetic resources in these taxa, that is, to identify candidate sites in the genome involved in responses to abiotic stress, and to understand the roles of gene flow and environmental differentiation in their divergence. This study highlights the importance of conserving these two species as phytogenetic resources for crop improvement.Summary Crop wild relatives (CWR) are reservoirs of genetic diversity and they are important for the maintenance of crop evolutionary potential. Mexico is the centre of domestication and diversity for many CWR. The genus Cucurbita originated in America, where at least six independent domestication events took place. Nonetheless, Cucurbita CWR have been seldom studied. In the present study, we test the role of incomplete lineage sorting (ILS) and secondary contact in the divergence of C. okeechobeensis subsp. martinezii and C. lundelliana. Additionally, we seek to understand the role of environmental differentiation in their divergence. We obtained 1,638 single nucleotide polymorphisms (SNPs) for 107 individuals from the most recently diverged wild species in this genus, encompassing 25 localities of C. lundelliana and nine localities of C. okeechobeensis subsp. martinezii in Mexico. We found higher genetic variation in C. lundelliana (HE = 0.227) than in C. okeechobeensis subsp. martinezii (HE = 0.187). Each taxon constitutes a well‐differentiated genetic group, with an area of introgression in Pantanos de Centla. We found candidate loci associated to hydric and thermal stress, which could be valuable for crop improvement. Our study supported a scenario of ILS followed by secondary contact, where divergence was probably driven by Pleistocene climate change. These CWR represent important phytogenetic resources for crop improvement given their high levels of genetic variation and differentiation and their SNPs associated to different climatic variables.

Agrooter® - Skillful Natural Tubes to Enhance Soil Productivity and Manage Hydric Stress

Agrooter® is a Hungarian patent created by the first author, tested in several field experiments in Hungary and recently in some African countries. The point of the invention is that small tubes are mixed into the fertile soil, which keeps moisture or air in the soil, depending on their position. In the case of an entirely random distribution of the tubes within the soil, the tubes achieve both advantages. The point of this patent is the carefully designed size and amount of empty tubes fabricated from organic bio-degradable materials and micro-grinded specific minerals. Using these tubes in the plantations helps to avoid hydric stress and increases yield and improves quality. These small particles improve the water budget (significantly decreases water demand) and enhance the soil’s porosity. After the description of the principle of the tubes, including the positive impact of the tubes on micro-biotic life in the soil, technical advice is also presented on the optimum amounts of mixing the tubes into the soil, and ways of planting are illustrated for plant seedlings and tree saplings. Finally, six different plants planted and observed in Hungary demonstrate the usefulness of these effects. The most common feature of these results is that significant improvements are obtained by using the Agrooter® compared to the traditional plantation by using a 50% smaller amount of irrigation water.

Comparative analysis of floral transition and floral organ formation in two contrasting species: Disocactus speciosus and D. eichlamii (Cactaceae)

Key messageContrasting morphologies in Disocactus are the result of differential development of the vegetative and floral tissue where intercalary growth is involved, resulting in a complex structure, the floral axis.Species from the Cactaceae bear adaptations related with their growth in environments under hydric stress. These adaptations have translated into the reduction and modification of various structures such as leaves, stems, lateral branches, roots and the structuring of flowers in a so-called flower-shoot. While cacti flowers and fruits have a consistent structure with showy hermaphrodite or unisexual flowers that produce a fruit called cactidium, the developmental dynamics of vegetative and reproductive tissues comprising the reproductive unit have only been inferred through the analysis of pre-anthetic buds. Here we present a comparative analysis of two developmental series covering the early stages of flower formation and organ differentiation in Disocactus speciosus and Disocactus eichlamii, which have contrasting floral morphologies. We observe that within the areole, a shoot apical meristem commences to grow upward, producing lateral leaves with a spiral arrangement, rapidly transitioning to a floral meristem. The floral meristem produces tepal primordia and a staminal ring meristem from which numerous or few stamens develop in a centrifugal manner in D. speciosus and D. eichlamii, respectively. Also, the inferior ovary derives from the floral meristem flattening and an upward growth of the surrounding tissue of the underlying stem, producing the pericarpel. This structure is novel to cacti and lacks a clear anatomical delimitation with the carpel wall. Here, we present a first study that documents the early processes taking place during initial meristem determination related to pericarpel development and early floral organ formation in cacti until the establishment of mature floral organs.

Smart Rainwater Harvesting Service Design

Megacities expansion around the globe have significantly increased the demand for resources to the point of depleting available reservoirs and requiring importing resources from nearby locations sometimes implementing major infrastructure projects for guaranteeing access to resources. This is particularly true for one of the largest Megalopolis in the world, Mexico City, that imports 40% of its water supply from neighboring states. Despite of being a city holding important hydric resources, the lack of strategies, technological innovation, user-centered service design, consistent data acquiring, and optimization tools have led to a poor water management that maintains the metropolis with a constant scarcity of the resource. Therefore, the aim of the current paper is to present a conceptual service design for smart rainwater harvesting in urban settlements that provides an alternative source for citizens to reduce the local hydric stress, diminish aquifer overexploitation and increase water well recovery contributing to more sustainable cities.

Arbuscular Mycorrhizal Fungi Colonization of Jatropha curcas Roots and Its Impact on Growth and Survival under Greenhouse-Induced Hydric Stress

Arbuscular mycorrhizal fungi (AMF) provide benefits to host plants by enhancing nutrition and overall fitness. In this study, AMF species were isolated from the soil rhizosphere of Jatropha curcas and were identified and evaluated for their potential in fostering the development of Jatropha seedlings within a controlled greenhouse environment. The first experiment assessed the interplay between hydric stress and AMF inoculation on mycorrhizal colonization. The next experiment examined the impact of quercetin on mycorrhizal colonization. Out of 204 glomerospores corresponding to 28 species spanning 10 genera, Acaulospora (14) and Scutellospora (5) were the most abundant taxa. Six new records of AMF for Costa Rica are reported. Mycorrhizal colonization was observed in 43.2% of Jatropha plants (34.7% by AMF typical hyphae; arbuscules 8.9%; coils 5.6%; and vesicles 5.4%). Significant survival effects due to AMF inoculation under hydric stress were observed. On day 85, non-mycorrhizal plants subjected to hydric stress showed a mere 30% survival rate, whereas their mycorrhizal counterparts under hydric stress exhibited survival rates of 80% and 100% with and without irrigation, respectively. Furthermore, plants with irrigation and mycorrhizas showed greater hydric stress tolerance and superior growth. The inoculated plants, irrespective of irrigation, demonstrated mycorrhizal colonization rates of 63% and 72%, respectively. Quercetin did not affect Jatropha’s growth, but there were differences in AMF root colonization. In summary, these findings accentuate the viability of a native consortium in augmenting Jatropha survival, warranting consideration as a potent biofertilizer within greenhouse settings. The AMF described can be used for Jatropha propagation programs.