Hydrazine Carboxamide Research Articles

Synthesis, Structural, DFT studies, Biological Effectiveness, and Molecular Docking Aspects of Multitarget Novel N-(4-Phenylthiazol-2-yl)hydrazine carboxamide Hybrids as COX inhibitors, Anti-TB, and Anti-oxidant activity

We herein report, a series of N-(4-phenylthiazol-2-yl)hydrazinecarboxamide based multitarget compounds 3(a-i) were designed and synthesized. Ten compounds 2, and 3(a-i) were characterized by FTIR, NMR (1H and 13C), and mass analysis and subsequently, tested for their potential COX inhibitors, anti-TB, and anti-oxidant activities. The physicochemical and ADME studies for newly synthesized compounds were disclosed. The DFT calculations were taken for the selected molecules using CAM-B3LYP hybrid functional with a 6–31+g(d) all-electron basis set using the Gaussian 09 package. The compounds 3d, 3e, and 3f recognized outstanding COX-II inhibitions with IC50 values of 0.62, 0.78, and 0.72 μM compared to standard drugs. The compounds 3d, 3e, and 3f showed outstanding anti-TB activity with a MIC value of 0.78μg/mL. The compound 3d, and 3f attested outstanding antioxidant activity at 10 μg/mL with a rate of inhibition of 68.12%, and 68.85% respectively. Finally, the molecular docking studies carried out with cyclooxygenase-2 (PDB ID: 6COX), M. tuberculosis enoyl reductase (INHA) (PDB ID: 4TZK), and cytochrome c peroxidase (PDB ID: 2×08), for all the newly synthesized derivatives. Finally, selected compounds were taken for their molecular dynamic studies.

Synthesis, characterization and bioassay of Cr(III), Mn(II), Fe(II), and Zn(II) complexes with (2E)-2-(4-methoxy benzylidene) hydrazine carboxamide

The ligand (2E)-2-(4-methoxybenzylidene)hydrazine carboxamide [L] has been synthesized from the reaction of 4-methoxybenzyaldehyde with semicarbazide. This ligand was characterized by elemental analysis, 1H-NMR spectra, FT-IR spectra and Uv-visible spectra.Metal complexes of this ligand were synthesized by reaction of metal ions [Cr(III), Fe(II), Mn(II) and Zn(II)] with this ligand [L]. All metal complexes were characterized by elemental analysis, Magnetic susceptibility, electrical conduc- tance, Uv-vis spectra and FT-IR spectra. The antimicrobial activity of the ligand and its metal complexes were investigated for their (In-Vitro) antibacterial on (E. coli) and (Bacillusubtilis) and antifungal (A. niger) and (P. chrysogenum) activities. The result indicates that the growth of the tested organism was inhibited by the most of this complexes.

Synthesis, Molecular Docking and In-silico Admet Screening of New Benzothiazole-Linked Pyrazole Prototype Derivatives: Validation of Resistant Strains and their Biological Activity

Background: The necessity for newer anti-microbial medications with prototypes has arisen as a result of the prevalence of infections caused by resistant strains of microorganisms. Objective: A series of nine novel benzothiazole-linked pyrazole prototype derivatives were synthesized in multistep reactions and evaluated for anti-microbial and anti-fungal activities. The druglikeness along with physicochemical properties of synthesized compounds were determined by docking the ligands with resistant strains. Methods: Synthesis of benzothiazole and pyrazole prototype derivatives was carried out by a sequence of reactions to attain the hydrazine carboxamide derivatives. All the synthesized compounds were characterized and evaluated for their anti-bacterial and anti-fungal activities against carbapenem-resistantresistant pseudomonas aeruginosa (CP-PA), carbapenem-resistantresistant Klebsiella pneumoniae (CP-KP), cephalosporin-resistantclostridium difficile (CR-CD), macrolideresistantresistant streptococcus pyogenes (MR-SP), Voriconazole-resistant Aspergillus Niger (VRAN) and Fluconazole-resistant Candida glabrata (FR-CG). Physico-chemical parameters were done by Medchem DesignerTM software version 5.5 and ADMET parameters by pkCSM online tool. Furthermore, molecular docking was accomplished with PyRx 0.8 by AutoDock Vina program. Results: All the synthesized derivatives were characterized and evaluated for their anti-bacterial activity, which shows the significant activity of 6i with MIC 36.17 μM on CP-PA, MIC 36.86 μM on CP-KP, MIC 38.45 μM on CR-CD, and MIC 37.09 μM on MR-SP, with respect to ciprofloxacin with an average of MIC 32 μM for all resistant bacterial strains. The prototype derivatives were also evaluated for their anti-fungal activity, in which derivative 6i was found to be significant with MIC 35.27 μM for VR-AN, MIC 34.78 μM for FR-CG, and MIC values of 25.60 μM and 27.08 μM for Nystatin for all fungal-resistant strains. In-silico predicted parameters for synthesized prototype derivatives stood to be drug-like. Conclusion: From the novel benzothiazole and pyrazole hybrid derivatives, compound 6i was found to be effective for anti-microbial and anti-fungal drugs and hence can be further explored for dual activities. Furthermore, derivatization was made to synthesize further potent derivatives for anti-microbial and anti-fungal treatments.

Exploring Novel Pyridine Carboxamide Derivatives as Urease Inhibitors: Synthesis, Molecular Docking, Kinetic Studies and ADME Profile.

The rapid development of resistance by ureolytic bacteria which are involved in various life-threatening conditions such as gastric and duodenal cancer has induced the need to develop a new line of therapy which has anti-urease activity. A series of pyridine carboxamide and carbothioamide derivatives which also have some novel structures were synthesized via condensation reaction and investigated against urease for their inhibitory action. Among the series, 5-chloropyridine-2 yl-methylene hydrazine carbothioamide (Rx-6) and pyridine 2-yl-methylene hydrazine carboxamide (Rx-7) IC50 = 1.07 ± 0.043 µM, 2.18 ± 0.058 µM both possessed significant activity. Furthermore, molecular docking and kinetic studies were performed for the most potent inhibitors to demonstrate the binding mode of the active pyridine carbothioamide with the enzyme urease and its mode of interaction. The ADME profile also showed that all the synthesized molecules present oral bioavailability and high GI absorption.

Synthesis, Characterization and Biological Activity of ONO Donor Schiff Base and its Metal Complexes

A new Schiff base ligand (E)-2-((7-hydroxy-4-methyl-2-oxo-2H-chromen-8-yl)methylene)-N-(5- methyl-3-phenyl-1H-indol-2-yl)hydrazine carboxamide (L) (3) was synthesized by the reaction of N-(5-methyl-3-phenyl-1H-indol-2-yl)hydrazinecarboxamide (1) and 7-hydroxy-4-methyl-2-oxo-2Hchromene- 8-carbaldehyde (2). The Cu(II), Co(II), Ni(II) and Zn(II) metal complexes (4a-d) were synthesized and its structural elucidation was done by different spectral techniques. The Schiff base (3) behaves as ONO donor ligand and forms the complexes of the sort [M(L)(Cl)(H2O)2] for Cu(II) (4a) and Zn(II) (4d) and [M(L)2] for Co(II) (4b) and Ni(II) (4c). Compounds (3) and (4a-d) were tested in vitro for antimicrobial action, cytotoxicity property against Artemia salina and anti-tuberculosis assay against Mycobacterium tuberculosis (ATCC 25177). The metal complexes showed very good biological activity.

Study of Hydrazine Carboxamide, Characterization Method and Biological Activities with Transition Metal Using Cu (II) and Mn (II) as Central Metal

The co-ordination chemistry of transition metals and their derivatives has received much attention in the recent years, primarily because of their biological importance1-3, Nitrogen, Oxygen and Sulphur donor ligands possess a range of biological applications like antitomour4, antiviral5, antimaterial7 and antifungal activities. The legends of Hydrazine with their metal complexes with Cu (II) and Mn (II). Fungicidal activity of the ligand and their metal complexes were also done to establish any relation with the metal and ligands.

Ultrasound promoted green synthesis, anticancer evaluation, and molecular docking studies of hydrazines: a pilot trial

We reported herein an efficient, environmentally friendly synthesis of hydrazine carboxamides (6a–l) in a water-glycerol (6:4) solvent system using ultrasonic irradiation. Ultrasonicated reactions were found to be much faster and more productive than conventional synthesis. The prepared compounds (6a–l) were tested against nine panels of 60 cancer cell lines according to the National Cancer Institute (NCI US) protocol. N-(4-Chlorophenyl)-2-(2-oxoindolin-3-ylidene)hydrazine-1-carboxamide (6b) was discovered to be promising anticancer agents with higher sensitivity against CCRF-CEM, HOP-92, UO-31, RMPI-8226, HL-60(TB), and MDA-MB-468 with percent growth inhibitions (%GIs) of 143.44, 33.46, 33.21, 33.09, 29.81, and 29.55 respectively. Compounds (6a–l) tested showed greater anticancer activity than Imatinib, except for compound 6k. Compounds 6b and 6c were found to be lethal on the CCRF-CEM leukaemia cell line, with %GIs of 143.44 and 108.91, respectively. Furthermore, molecular docking analysis was performed to investigate ligand binding affinity at the active site of epidermal growth factor (EGFR).

Spectroscopic studies, DFT calculation, electronic properties and antimicrobial studies on 2-(2-(naphthalene-2-yloxy)-1-phenylethylidene) hydrazine carboxamide

We report the 2-(2-(naphthalene-2-yloxy)-1-phenylethylidene) hydrazinecarboxamide (NAPCAR) compound has been synthesized from 2-(naphthalene-2-yloxy)-1-phenylethanone (2-NAPETH), the parent compound. The characterization of both the parent and product has been done using FT-IR, 1H NMR and 13C NMR spectroscopic and theoretical studies. Solid double bonds have been identified near some of the aromatic ring carbons instead of the partial double bond in both the parent and the title compounds. The Highest Occupied Molecular Orbital (HOMO) is found to be concentrated mainly on the naphthyl ring in both 2- NAPETH and NAPCAR. However, major portion of Lowest Unoccupied Molecular Orbital (LUMO) has been found to be spread over the naphthyl ring of NAPCAR unlike the2- NAPETH which has LUMO on the benzyl ring. The electrostatic potential surface (ESP) shows that both the 2- NAPETH and NAPCAR have nucleophilic site only. This NAPCAR is found to be against the pathogenic bacteria and fungi indicating that this compound may be considered as a candidate to be used in the development of new drugs capable of treating microbial infections.

Ultrasound promoted synthesis of N-(substituted phenyl)-2-(7‑hydroxy-4-methyl-2H-chromen-2-ylidene)hydrazine-1-carboxamides as cytotoxic and antioxidant agents

New N-(substituted phenyl)-2-(7‑hydroxy-4-methyl-2H-chromen-2-ylidene)hydrazine-1-carboxamides (5a-l) was synthesized in two steps using environmentally friendly ultrasound irradiation. The first step involved solvent free synthesis of 7‑hydroxy-4-methyl-2H-chromen-2-one (3) by using catalytic amount (10% mol) of FeCl3. The second step involved condensation of intermediate 3 and hydrazine carboxamide HCl analogues (4a-l) under ultrasonic influence in the water-ethanol (2:1) solvent system to obtain the title compounds (5a-l) in good yield. The structure of prepared compounds (5a-l) was confirmed by spectroscopic study. The synthetic strategy described here has many advantages, including a simple procedure and high conversion with quick reaction times. The cytotoxicity was tested on 60 NCI cancer cell lines at 10 µM and N-(4-chlorophenyl)-2-(7‑hydroxy-4-methyl-2H-chromen-2-ylidene)hydrazine-1-carboxamide (5b) showed promising activity with maximum sensitivity against CCRF-CEM, UO-31, RPMI-8226, NCI-H522, and HL-60(TB) with growth percent (GP) of 17.50, 79.67, 80.59, 82.35, and 83.70 respectively. In a DPPH free radical scavenging assay, the compounds 5b and 5d showed promising antioxidant activity with IC50 values of 16.32±1.29 and 14.28±1.82 M, respectively. The binding affinity of the target ligands (5a-l) against the active site of FGFR was also investigated using molecular docking. Within the active site of FGFR4, the ligand 5b interacted with four H-bonds with Asp630 (carbonyl function), Glu520 (with amide NH and ArNH), and Ile609 (phenol function), while an imine “N” showed two salt bridges with Asp630 and Glu520.

A Chromone Based Fluorescent Probe for the Effective Detection of Aluminium Ion.

A new chromone moiety fused with phenyl hydrazine carboxamide as a binding site for metal ion was designed and synthesized as a colorimetric/fluorimetric probe for selective sensing of Al3+ ion. The absorption and fluorescence spectral results showed selective and sensitive recognition of Al3+ with no significant interference from other competitive metal ions. The addition of Al3+ to R brought a prompt color change from colorless to yellow and the turn-on fluorescence response with Al3+ among different cations was studied. The visible color change and turn-on fluorescence Al3+ ion with R was attributed to the intra molecular charge transfer transition. The plausible binding nature of the receptor with Al3+ has also been anticipated by the results of 1H NMR and mass spectral analysis. For the practical effectiveness, test strip prepared using probe R was successfully applied to monitor the presence of Al3+ ion in aqueous medium.

Intracellular and Extracellular Zinc Detection by Organic Fluorescent Receptor

Keeping in view the ever growing demand and application of the organic small molecules based sensitive and selective fluorescence detection strategies for the trace metallic ions in the ecosystem, fluids and inside intracellular media, the present literature survey was focused on the recent development on the organic skeleton based fluorescence sensor for the zinc ion as Zn2+ is the second most abundant transition metal after iron in human body. The prominent organic based skeletons introduced during the past three years for zinc detection including azine, ((Z)-N´-(quinolin-2-ylmethylene)furan-2- carbohydrazide), nicotinohydrazide, hydrazone, phenolic cage, 4-methyl-2,6-bis[(E)-(2- (4-phenylthiazol-2-yl)hydrazono)methyl]phenol, bipyridine, N-(quinoline-8-yl)pyridine-2- carboxamide, anthracene, Schiff base, salen, helicene, Carbon Quantum Dots (CDs) functionalized with Calix[4]arene, coumarin, diaminomaleonitrile, peptide, hydroxypyrazole, salicylhydrazide were discussed in detail with particular focus on ligand-zinc complexation mechanism, UV-visible and fluorescence investigation, spectral variation, isosbestic emergence, limit of detection, ligand-zinc binding stoichiometry, association/binding constant and applications for intracellular tracing of metallic contamination via confocal fluorescence microscopic studies. Among the several discussed optical probes, rhodamine and fluorescein based material offer appreciable sensitivity, exhibiting drawback of pH sensitivity. Probes based on these ligands triggered “turn-on” signal even in the absence of metals upon fluctuation in pH e.g., acidic in former case and basic in the latter case. Hydroxypyrazole-based ligands also showed detection signal variation by switching the pH of the solution. Schiff base and bipyridyl scaffold were found to possess good ligation toward the several transition metals. Azole, oxazole, thiazole, thiadiazole, hydrazine carboxamide and hydrazine carbothiomide are the bioactive molecules exhibiting good cell viability and probes designed by using these central nucleus might be better to invest for intracellular imaging. Symmetrical heterocyclic cage like probe showed better chelation toward several transition metals and it is a good choice for the design and development of sensor for simultaneous detection of several transition metals.

Synthesis, Structure, and Biological Activity of Coordination Compounds of Cobalt(II), Nickel(II), and Copper(II) with N-(Methoxyphenyl)-2-[(5-nitrofuryl)methylene]hydrazine Carbothioamides

4-(2-Methoxyphenyl)-, 4-(3-methoxyphenyl)-, and 4-(4-memoxyphenyl)-2-[(5-mttofuryl)methylene]-hydrazine carboxamide (HL1–3) react with hydrates of cobalt (nickel, copper) chloride (nitrate, acetate) with the formation of the M(HL1–3)2X2 (M = Co2+, Ni2+, Cu2+; X = Cl−, NO3−) and M(L1–3)2 (M = Ni2+, Cu2+) coordination compounds. Structure of the obtained compounds has been studied by means of X-ray diffraction analysis. Their antimicrobial and antifungal activity towards a series of Staphylococcus aureus, Escherichia coli, and yeast-like fungi standard strains has been investigated.

Comprehensive Fractionation of Antioxidants and GC-MS and ESI-MS Fingerprints of Celastrus hindsii Leaves.

Background: In this study, column chromatography was applied to separate active fractions from the ethyl acetate extract of Celastrus hindsii, a medicinal plant widely used in Southern China, Northern Vietnam, Myanmar, and Malaysia. Methods: Fourteen fractions from different dilutions of chloroform and methanol were separated by column chromatography and examined for biological activities. Results: It was found that a dilution of 50–70% methanol in chloroform yielded the highest total phenolics, flavonoids, and antioxidant activities (1,1-dipheny1-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ehtylbenzothiazoline-6-sulfonic acid), diammonium salt (ABTS) radical scavenging activity, and β-carotene bleaching method measured by lipid peroxidation inhibition). In addition, by gas chromatography-mass spectrometry (GC-MS) and electrospray ionization-mass spectrometry (ESI-MS) analyses, fifteen principal compounds from bioactive fractions belonging to fatty acids, amides, flavonoids, sterols, terpenes, and phenols were identified. Of these compounds, α-amyrin, β-amyrin, hydrazine carboxamide, hexadecanoic acid, fucosterol, (3β)-D:C-friedours-7-en-3-ol, rutin, and 2-hydroxy-1-ethyl ester accounted for maximal quantities, whilst concentrations of other constituents were <5%. Conclusions: It is suggested that these identified compounds may greatly contribute to the antioxidant capacity of C. hindsii as well as its potential pharmaceutical properties.

Novel benzothiazole hydrazine carboxamide hybrid scaffolds as potential in vitro GABA AT enzyme inhibitors: Synthesis, molecular docking and antiepileptic evaluation.

In the present study, a series of newer benzothiazole derivatives containing thiazolidin-4-one (5a-g) and azetidin-2-one (6a-g), were synthesized by the cyclization of benzothiazolyl arylidene hydrazine carboxamide derivatives with thioglycolic acid and chloroacetyl chloride, respectively. Results of in vivo anticonvulsant screening revealed that compounds having 2,4-dicholoro (5c and 6c) and 4-nitro substituent (5g) at the phenyl ring have promising anticonvulsant activities without any neurotoxicity. Selected compounds were also evaluated for their in vitro GABA AT inhibition. The results indicated that compound 5c (IC50 15.26 μM) exhibited excellent activity as compared to the standard drug vigabatrin (IC50 39.72 μM) suggesting the potential of these benzothiazole analogues as new anticonvulsant agents.

Structure-Activity Relationships for Itraconazole-Based Triazolone Analogues as Hedgehog Pathway Inhibitors.

The Food and Drug Administration-approved antifungal agent, itraconazole (ITZ), has been increasingly studied for its novel biological properties. In particular, ITZ inhibits the hedgehog (Hh) signaling pathway and has the potential to serve as an anticancer chemotherapeutic against several Hh-dependent malignancies. We have extended our studies on ITZ analogues as Hh pathway inhibitors through the design, synthesis, and evaluation of novel des-triazole ITZ analogues that incorporate modifications to the triazolone/side chain region of the scaffold. Our overall results suggest that the triazolone/side chain region can be replaced with various functionalities (hydrazine carboxamides and meta-substituted amides) resulting in improved potency when compared to ITZ. Our studies also indicate that the stereochemical orientation of the dioxolane ring is important for both potent Hh pathway inhibition and compound stability. Finally, our studies suggest that the ITZ scaffold can be successfully modified in terms of functionality and stereochemistry to further improve its anti-Hh potency and physicochemical properties.

Assessment of Bioenergy Potential of Lemon Grass

Grasses can serve as an alternative biomass for the production of bioenergy which plays a vital role in solving some of the challenges faced in the production of renewable energy and also help in tackling some of the environmental challenges faced all over the world. In this research, the phytochemical and biofuel content of Cymbopogon citratus was assessed. The phytochemical screening and proximate analysis were carried out according to standard qualitative and quantitative methods. Alkaloids, balsam, flavonoids, glycosides, saponin, carbohydrates, protein, volatile oil, minerals are analyzed. Bioenergy was produced using enzymatic hydrolysis after pretreatment under different pH viz: pH 5, pH 7 and pH 8 all under an ambient temperature of 36˚C. Each of the pretreated samples of varying pH was then fermented using Saccharomyces cerevisiae. Each of the pre-treatment under three different pH was made in triplicate. The absorbance was determined for reducing sugar and at the end of the production, the pretreated samples were subjected to Gas chromatography and mass spectroscopy to analyze the end product of bioenergy produced. The result for phytochemicals shows the presence of flavonoids and alkaloids (1.60%) followed by volatile oil (7%), saponin (3.20%) and tannins (0.6%). The result for proximate analysis shows that lemon grass has the highest percentage of carbohydrates (87.63%). This was followed by ash (11.67%), protein (11.14%) and fibres (2.83%). However, the mineral analysis shows the presence of Calcium (1.88 mg/ml), Magnesium (0.13 mg/ml), Phosphorous (5.87 mg/ml), Potassium (2866.67 mg/ml) and Sodium (89.17 mg/ml). The absorbance in C. citratus is higher at pH 5 (0.0651). The GC-MS analysis of the bioenergy produced shows the presence of Ethyl alcohol (bioethanol) in all the samples at different pH. Hydrazine carboxamide was present in all the treatments under different pH. In addition glycidol, acetyldehyde, acetic acid, 1,2-propanediamine were found present, having fuel potential and are good source of gasoline. These are produced as a result of fermentation and enzymatic activities of the organic compound present in the biomass sample used.

Design, synthesis and biological evaluation of novel 4-anlinoquinazoline derivatives as EGFR inhibitors with the potential to inhibit the gefitinib-resistant nonsmall cell lung cancers

A series of quinazoline derivatives with benzylidene hydrazine carboxamide were designed and synthesised as EGFR inhibitors. Most compounds exhibited exceptional anti-proliferative activity against A549, HepG2, MCF-7 and H1975 cells. Furthermore, six compounds demonstrated excellent inhibition activity against EGFRWT with the IC50 value both less than 2 nM. Among the six compounds, 44 exhibited the strongest activity (0.4 nM) and potently inhibited EGFRL858R/T790M (0.1 μM). Excitingly, the most potent compound 14 showed excellent enzyme inhibitory activity with 6.3 nM and 8.4 nM for both EGFRWT and EGFRT790M/L858R. The result of AO single staining and Annexin V/PI staining showed that the compound 14 and 44 could induce remarkable apoptosis of A549 cells. The compound 14 arrested the cell cycle at the S phase and compound 44 arrested the cell cycle at the G0 phase in A549 cells. These preliminary results demonstrate that compound 14 and 44 may be promising lead compound-targeting EGFR.

AIE based “on-off” fluorescence probe for the detection of Cu2+ ions in aqueous media

A simple hydrazine carboxamide derivative with intramolecular charge transfer and aggregation induced emission (AIE) properties have been designed and synthesized. The derivative (Probe 1) shows typical AIE characteristic emission in THF-water mixtures and emits yellowish orange color on reaching the water fraction 98%. The probe molecule show selective sensing response for Cu2+ ions via fluorescence turn-off mechanism in aqueous media over other metal ions. The emission changes towards Cu2+ ions that could be clearly observed by the naked eyes under 365 nm UV lamp promote the probe molecule as a promising candidate for practical utilization. Furthermore, from the fluorescence titration data, the detection limit for Cu2+ ions was found to be 0.44 μM.

Synthesis, Characterization and Antimicrobial Activity of 2-[1-(2,4-Dihydroxyphenyl)Ethylene]Hydrazine Carboxamide and its Zn(II) Metal Complex

The synthesis of 2-[1-(2,4- dihydroxyphenyl)ethylene]hydrazinecarboxamide and its Zn(II) metal complex was achieved. 2, 4 dihydroxy acetophenone is synthesized by resorcinol with glacial acetic acid. This further treated with semicarbazide hydrochloride to form its semicarbazone. Zn(II) metal complex was prepared by dissolving equimolar quantities of metal salt and Schiff base ligand in ethanol. All the target compounds were characterized by M.P, TLC and UV-visible and IR spectral data. Antimicrobial activities of these synthesized compounds were studied in sterile saline by Agar well diffusion method against M. luteus, B. subtilis, S. aureus, E. coli, P. aeruginosa bacteria. All the synthesized compounds have shown good to moderate antimicrobial activity.

Synthesis, Characterization and Antimicrobial Activity of 2-[1-(2,4-Dihydroxyphenyl)Ethylene]Hydrazine Carboxamide and its Zn(II) Metal Complex

The synthesis of 2-[1-(2,4- dihydroxyphenyl)ethylene]hydrazinecarboxamide and its Zn(II) metal complex was achieved. 2, 4 dihydroxy acetophenone is synthesized by resorcinol with glacial acetic acid. This further treated with semicarbazide hydrochloride to form its semicarbazone. Zn(II) metal complex was prepared by dissolving equimolar quantities of metal salt and Schiff base ligand in ethanol. All the target compounds were characterized by M.P, TLC and UV-visible and IR spectral data. Antimicrobial activities of these synthesized compounds were studied in sterile saline by Agar well diffusion method against M. luteus, B. subtilis, S. aureus, E. coli, P. aeruginosa bacteria. All the synthesized compounds have shown good to moderate antimicrobial activity.