Saussurea superba Anthony belongs to the Compositae family and is one of the major medicinal plants in China. Its roots have long been used as folk medicine to treat influenza, pharyngeal swelling, measles, urticaria, and food poisoning [1]. The present research deals with the isolation and elucidation of compounds 1–20 from the whole plant of S. superba. The compounds isolated were identified as follows: stigmast-1,4-dien-3-one (1) [2], stigmast-4-en-3-one (2) [3], 6 -hydroxystigmast-4-en-3-one (3) [3], -sitosterol (4) [3], sitosterol-5-en-3 ,7 -diol (5) [3], 3 -hydroxystigmast-5-en7-one (6) [3, 4], 5,6 -epoxy-5 -sitostan-3 -ol (7) [5], 5,6 -epoxy-5 -sitostan-3 -ol (8) [12], 5 ,8 -epidioxy-24(S)methylcholesterol-6,22-dien-3 -ol (9) [6], lupeol (10) [7, 8], -amyrin (11) [9], 3 -hydroxy-urs-12-en-11-one (12) [10], luteolin (13) [11], luteolin-3 -O-D-glucopyranoside (14) [12], umbelliferone (15) [13], scopoletin (16) [14], skimmin (17) [13, 15], scopolin (18) [13, 15], 2-hydroxy-4-acetoanisole (19) [16], and phenylacetic acid (20) [17]. These compounds were characterized by spectral data and comparison with those reported in previous work. Plant Materials. The whole plant of Saussurea superba was collected in Huzhu county, Qinghai province, P. R. China, in August 2002. It was identified by Dr. Huan-Yang Qi. The voucher specimens (2002002) were deposited in the Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, P. R. China. Extract, Isolation, and Purification. The dried plant material (2.0 kg) was powdered and extracted with 95% of industrial ethanol at 60 C for 24 h and then evaporated. The residue (120 g) was mixed with 500 mL water and extracted with petroleum ether, EtOAc, and n-butanol, respectively, to yield three parts (A, B, and C). Part A (30 g) was chromatographed on a silica gel column, eluting with petroleum ether (60–90 C)–acetone (v/v = 30:1, 20:1, 15:1, 10:1, 5:1, 3:1, 1:1, each about 3.0 L), to give four fractions (A1–A4) after concentration at reduced pressure. Fraction A1 was applied to a silica gel column, eluting with petroleum ether–EtOAc (v/v, from 30:1 to 25:1), to yield compounds 19 (600 mg), 4 (168 mg), and 10 (20 mg). Fraction A2 was eluted on a silica gel column with CHCl3–acetone (40:1) to give compounds 1 (7 mg ) and 3 (5 mg) after purified by pre-TLC. Compounds 2 (27 mg) and 11 (4 mg) were subsequently crystallized from fraction A3, eluting with CHCl3–acetone (20:1). Fraction A4 was eluted on a silica gel column with CHCl3–EtOAc (15:1) to afford a crude mixture of 5, 9, and 6 (10 mg) and 12 (3 mg). The mixture was processed by pre-TLC to give crystals 5 (2 mg) and 9 (7 mg). Part B (20 g) was eluted on a silica gel column with petroleum ether–EtOAc (6:1) and petroleum ether–acetone (v/v, from 10:1 to 7:1) to afford compounds 13 (14 mg), 15 (1 g), and 16 (14 mg), and then by pre-TLC (petroleum ether–EtOAc, 8:1) to give white solids 20 (120 mg), 7 (2 mg), and 8 (2 mg). Part C (54 g) was applied to a silica gel column, eluting with a CHCl3–CH3OH gradient to yield 14 (272 mg), 17 (19 mg), and 18 (7 g), respectively.
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