Humoral Components Of Immune System Research Articles

Interrelation of immunological and hemodynamic parameters of the fetoplacental system in exacerbation of chronic bronchitis of cytomegalovirus etiology

Aim. To establish the relationship of immunological and hemodynamic parameters of the fetoplacental system during exacerbation of chronic bronchitis of cytomegalovirus etiology. Materials and methods. A study was carried out of the concentration of TNFα, IgA, IgM in the blood serum and the systolic-diastolic ratio (SDR) in the right uterine artery (RUA) in 80 women in the second trimester of pregnancy, uncomplicated and complicated by exacerbation of cytomegalovirus infection (CMVI). Three groups were retrospectively distinguished. The first group included 30 patients with CMV seronegative physiological pregnancy, the second group included 25 women with exacerbation of chronic bronchitis caused by CMVI reactivation, initiating the development of chronic compensated placental insufficiency (CCPI), in the third group – 25 pregnant women with chronic bronchitis in the acute stage, induced by CMVI, leading to the formation of chronic subcompensated placental insufficiency (CSPI) in the third trimester of gestation. Results. In the first group, the concentration of TNFα in the blood serum was Me 16.4 (13.1–33.8) pg/mL, IgA – Me 2.5 (2.23–2.62) mg/mL, IgM – Me 0.99 (0.87–1.56) mg/mL, and SDR in RUA – Me 1.95 (1.87–2.30). In the second group, compared with the first one, there was an increase in the concentration of TNFα by 4.95 times (p=0.000001), IgM − by 2.51 times (p = 0.000001) and SDR in RUA by 1.25 times (p = 0.000001) with a 1.49-fold decrease in the IgA level (p = 0.000001). In the third group, compared with the first one, there was an increase in TNFα by 6.12 times (p = 0.000001), IgM − by 2.98 times (p = 0.000001) and the value of SDR in RUA by 1.70 times (p = 0.000001) against the background of a 2.31-fold decrease in IgA concentration (p = 0.000001). In patients of the third group, in comparison with the second group, higher levels of TNFα (by 1.23 times, p = 0.000001), IgM (by 1.18 times, p = 0.000001) and SDR in the RUA (by 1.35 times, p = 0.000001), as well as lower values of the IgA concentration (1.54 times, p = 0.000001). Conclusion. In women with exacerbation of chronic bronchitis caused by CMVI reactivation, leading to the formation of CSPI, in contrast to patients with a similar bronchopulmonary pathology of cytomegalovirus origin and the development of CCPI, a more pronounced activation of the systemic inflammatory response and an imbalance in the humoral component of immune system lead to an increase in vascular resistance in the RUA pool.

AGE-DEPENDENT INDEXES OF IMMUNITY IN THE PATIENTS WITH PSORIATIC ARTHRITIS

Psoriatic arthritis is a chronic progressive systemic inflammatory disease of joints and spine, which leads to the development of erosive arthritis, bone resorbtion, multiple enthesitis and spondylitis. Severe clinical course, resistance to therapy, high prevalence of disability, increased mortality of patients determine a need for further study of the disease. Psoriatic arthritis is a multifactorial disease including immune pathogenic factors representing a complex process of interaction between cellular and humoral components of immune system. The most important way of activating epidermal cells and synovial membrane proliferation in psoriatic arthritis is an imbalance of proinflammatory and anti-inflammatory cytokines. It is noted that the features of clinical course in psoriasis are age-dependent. The study of immune response indices in different age groups allows to reveal distinct progression features of the psoriatic pathology.The purpose of this study was to compare concentrations of proinflammatory and anti-inflammatory cytokines, cellular and humoral immunity, and to conduct a comparative analysis in young and adult patients with psoriatic arthritis.The study included a group of patients with psoriatic arthritis (n = 101) who were divided by their age: group 1, from 18 to 44 years (n = 43); group 2, over 44 years (n = 58). The control groups (3 and 4) included virtually healthy people (n = 103) matched for sex and age with the patients. Populational and subpopulation profiling of blood lymphocytes was performed by flow-cytometry using monoclonal antibodies to CD3, CD4, CD8, CD16, CD19 (LLC “Sorbent”, Moscow, Russia). Phagocytic activity of peripheral blood neutrophils was assessed microscopically by uptake of latex particles. Concentrations of IgA, IgM, and IgG immunoglobulins, circulating immune complexes, cytokines (IL-4, IL-6, IL-10, TNFα) in blood serum was determined by enzyme-linked immunosorbent assay. Statistical evaluation of the results obtained was performed using applied “Statistica 6.0” software.In all the age subgroups of patients with psoriatic arthritis, we have revealedstatistically significant differences against controls, i.e., increased relative and absolute number of CD3-CD16+lymphocytes in peripheral blood, higher concentration of CIC-C1q, with decreased concentrations of IgA, IgM, IgG in blood serum. The analysis of the main cellular and humoral indicators of immunity in psoriatic arthritis patients revealed a statistically significant differences for psoriatic arthritis in young and adulthood. E.g., the serum concentration of IL- 10 was statistically significantly lower in psoriatic arthritis at a young age in comparison with adult psoriatic arthritis. Phagocytic number and IgG concentration in serum were statistically significantly lower in adults with psoriatic arthritis adulthood than in young patients.In conclusion, The revealed changes in immunological indices in psoriatic arthritis in young and adult patients indicate to differences against healthy controls, as well as intergroup differences. Previous studies have not revealed statistically any significant differences in immunological parameters between young and adult patients with psoriatic arthritis in, thus suggesting a presence of immune disorders associated with psoriatic pathology, but not with the age of patients. However, changes of immunological reactivity are observed with increasing age of patients with psoriatic arthritis and development of severe clinical forms, and they can be considered as markers of psoriatic disease progression, such as increased concentrations of IL-10 and lower IgG amounts in blood serum, a decreased phagocytic number.

ДОСЛІДЖЕННЯ ГУМОРАЛЬНОЇ ЛАНКИ ІМУННОЇ СИСТЕМИ У ПОСТРАЖДАЛИХ ІЗ ТРАВМАТИЧНИМИ ПЕРЕЛОМАМИ НИЖНЬОЇ ЩЕЛЕПИ РІЗНОГО ПСИХОСОМАТИЧНОГО ТИПУ ОСОБИСТОСТІ

У регуляції перебігу репаративних процесів у кістковій тканині й виникненні гнійних ускладнень визначальну роль відіграє імунна система. Останніми роками з’явились дослідження, в яких встановлено кореляцію між частотою виникнення гнійних ускладнень і психосоматичним типом особистості. Однак публікацій, у яких висвітлювались би особливості порушень гуморальної ланки імунної системи залежно від психосоматичного типу особистості постраждалих із переломами нижньої щелепи, ми не знайшли.Мета дослідження – вивчити функціональні порушення гуморальної ланки імунної системи у постраждалих із травматичними переломами нижньої щелепи (ТПНЩ) залежно від психосоматичного типу особистості.Матеріали і методи. 60 постраждалих осіб чоловічої статі, у яких діагностовано травматичні односторонні відкриті переломи нижньої щелепи в ділянці кута, поділили на дві групи: перша група – екстраверти (36 осіб) й друга – інтроверти (24 особи) згідно з класифікацією Айзенка. Матеріалом для проведення імунологічних досліджень слугувала венозна кров. Концентрацію імуноглобулінів класів A, M, G визначали методом G. Mancini в динаміці на 1-й, 3-й, 7-й дні після травми.Результати досліджень та їх обговорення. Встановлено підвищення кількості СD 22+-лімфоцитів упродовж усього терміну спостереження у постраждалих обох досліджуваних груп. Вміст IgG у перші дні після травми був підвищеним у обох досліджуваних групах, а на 7-й день його вміст нормалізувався тільки у постраждалих пешої групи, а другої ставав нижчим від показника контрольної групи. Кількісні зміни IgA однотипні в обох досліджуваних групах – підвищений рівень у перші дні після травми, а до 7-го дня дослідження ставав нижчим від контролю у обох групах, що вказує на депресію даної ланки імунітету. Встановлене підвищення вмісту IgM у обох досліджуваних групах упродовж усього терміну спостереження було найбільш виражене в ранній період після травми.Висновки. Отримані результати свідчать про напруження в роботі гуморальної ланки імунної системи, яке проявляється підвищенням концентрації усіх класів імуноглобулінів, особливо виражене у перші дні після травми. У постраждалих осіб-інтровертів кількісні зміни всіх класів імуноглобулінів більш виражені.

Some immunophysiological features of sports activity under climatic and geographical conditions of the middle Ob region

We analyzed the cellular and humoral.components of immune system in athletes who were born, lived and trained in the conditions of the Middle Ob Region (Khanty-Mansi Autonomous Okrug - Ugra). The main group included young males and females engaged in cross country skiing (12 males, 10 females) and volleyball (11 males, 10 females). The control group (normal inhabitants of the region) consisted of 10 males and 10 females who were born and currently live in. KMAO-Ugra and do not do any sports on a regular basis. The age of subjects ranged from 19 to 22 years. It was found that the indices of cell and humoral components of immune system differed slightly between young women in the control and main groups. As for men, a significant decrease in total leukocyte, neutrophil and lymphocyte counts were observed; a decrease in the parameters of humoral immunity (b-lymphocytes and IgA) was also observed in male skiers. It was found that the total leukocyte count, the level of B-lymphocytes and the concentration of IgG in men who live in the Middle Ob region and do not do any sports is higher than in women of the same group.

Study of immunogenic properties of transmissible gastroenteritis virus antigen conjugated to gold nanoparticles

There was performed the study of immunogenic properties of the transmissible gastroenteritis virus antigen conjugated to gold nanoparticles. In comparative immunobiological studies there was found that immunization of guinea pigs driven by the colloidal gold conjugated to transmissible gastroenteritis virus antigen of swine, leads to activation of the respiratory activity of lymphoid cells and peritoneal macrophages, which is directly related to increased activity of antibody-producing cells and activation of antibody generating. The obtained data suggest that the colloid particles promote antigen presentation to the reticuloendothelial system organs. In addition, there was established that these carriers stimulate production of proinflammatory cytokines, which leads to a complete and consistent immune response of both cellular and humoral components of immune system.

ANA HEp-2 cells image classification using number, size, shape and localization of targeted cell regions

The ANA HEp-2 medical test is a powerful tool in autoimmune disease diagnostics. The last step of this test, the interpretation of immunofluorescent images by trained experts, represents a potential source of errors and could theoretically be replaced by automated methods. Here we present a fully automatic method for recognition of types of immunofluorescent images produced by the ANA HEp-2 medical test. The proposed method makes use of the difference in number, size, shape and localization of cell regions that are targeted by the antinuclear antibodies – the humoral components of immune system that bind human antigens as a result of the immune system malfunction. The method extracts morphological properties of stained cell regions using a combination of thresholding-based and thresholding-less approaches and applies a conventional machine-learning algorithm for image classification.

Immunologic Alterations in Long-Term Divers

Event Abstract Back to Event Immunologic Alterations in Long-Term Divers Hassan Abolhassani1*, Asghar Aghamohammadi1, Nima Rezaei1, Khodayar Ghorban2, Banafsheh Dormanesh3 and Maryam Dadmaneh2 1 Tehran University of Medical Science, Research Center for Immunodeficiency,Children's Medical Center Hospital, Pediatrics Center of Excellence, Iran 2 AJA University of Medical Sciences, Department of Immunology, Iran 3 AJA University of Medical Sciences, Pediatric Nephrology, Iran Diving could encounter the immune system of divers due to long term stressors and disasters. This study was designed to understand comprehensive effect of diving on the cellular and humoral components of immune system in a number of persons with long-term exposures. The cross-sectional study was developed on 25-male-expert divers. The immunologic parameters were compared in these individuals with 25 sex-age-matched healthy controls. Serum levels of IgA(p<0.001)and IgM(p=0.003)were significantly decreased in divers compared to the controls; while the levels of IgG and IgE(p<0.001)were significantly increased. Absolute count of B cells was significantly lower in the divers group, compared to the controls(p<0.001). Specific-antibody-responses of divers was also defected(p<0.01).An increased activity of classic pathway of complement components was detected in the divers(p<0.001). Absolute count of natural killer cells in the divers was also significantly higher than the controls(p<0.001).Humoral, cellular and complement components of the immune system could be involved during long-term diving. Keywords: Diving, Immune function, Infection, secondary immunodeficiency, hyperbaric oxygen exposure Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Abolhassani H, Aghamohammadi A, Rezaei N, Ghorban K, Dormanesh B and Dadmaneh M (2013). Immunologic Alterations in Long-Term Divers. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00377 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Hassan Abolhassani, Tehran University of Medical Science, Research Center for Immunodeficiency,Children's Medical Center Hospital, Pediatrics Center of Excellence, Tehran, 14194, Iran, hassan.abolhassani@ki.se Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract Supplemental Data The Authors in Frontiers Hassan Abolhassani Asghar Aghamohammadi Nima Rezaei Khodayar Ghorban Banafsheh Dormanesh Maryam Dadmaneh Google Hassan Abolhassani Asghar Aghamohammadi Nima Rezaei Khodayar Ghorban Banafsheh Dormanesh Maryam Dadmaneh Google Scholar Hassan Abolhassani Asghar Aghamohammadi Nima Rezaei Khodayar Ghorban Banafsheh Dormanesh Maryam Dadmaneh PubMed Hassan Abolhassani Asghar Aghamohammadi Nima Rezaei Khodayar Ghorban Banafsheh Dormanesh Maryam Dadmaneh Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.