Human Stem Cell Conditioned Medium Research Articles

Regenerative effect of combined laser and human stem cell-conditioned medium therapy on hypertrophic burn scar

PurposeThis study aimed to determine the effect of combined treatment with non-ablative laser and human stem cell-conditioned medium (HSCM) on tissue regeneration after burn-induced hypertrophic scar (HTS) formation. MethodsFourteen patients with HTSs on both sides of the same body part were subjected to three sessions of non-ablative laser treatment, with an interval of four weeks between each treatment. Following laser treatment, HSCM and normal saline were applied to the HTSs of the right (experimental) and left side (control), respectively. Over the next 6 days, HSCM and moisturizer were applied to experimental scars, while only moisturizer was applied to control scars. Skin characteristics were evaluated before laser treatment and on the seventh day after the third laser treatment. ResultsNo significant intergroup differences were noted in the initial evaluation (P > 0.05). We found significant differences between the pre- and post-treatment measurements of erythema (P < 0.001), trans-epidermal water loss (TEWL; P < 0.001), and Cutometer® parameters (all parameters; P <0.05) of experimental scars. Control scars also showed significant differences between pre- and post-treatment measurements of thickness (P = 0.01), erythema (P < 0.001), TEWL (P < 0.001), and Cutometer® parameters (all parameters; P < 0.05). Changes (pre- to post-treatment) in scar thickness between the experimental (−0.003 ± 0.09) and control scars (0.04 ± 0.12) were significant (P = 0.01). ConclusionThese results suggest that HSCM has a positive effect on short-term results when applied after laser treatment of hypertrophic scars.

Statement of Retraction: Skin rejuvenation by microneedle fractional radiofrequency and a human stem cell conditioned medium in Asian skin: a randomized controlled investigator blinded split-face study

Statement of Retraction: Skin rejuvenation by microneedle fractional radiofrequency and a human stem cell conditioned medium in Asian skin: a randomized controlled investigator blinded split-face study

A study on clinical effectiveness of cosmetics containing human stem cell conditioned media

BackgroundWith the steadily increasing trend of functional cosmetics consumption, the public is increasingly expecting the efficacy of cosmetics, and in order to meet these consumer needs, the development of various effective raw materials, as well as products through technical convergence research, has been activating focusing on dermatology. In the Republic of Korea, much attention was paid to cosmetic raw materials using human stem cells as a new growth engine at the end of the 2000s. Accordingly, the Ministry of Food and Drug Safety implemented the Regulations on Cosmetic Safety Standards, etc., which contains the safety standards of human-derived cell and tissue culture as raw materials for cosmetics.MethodsTwice a day, morning and evening, for 28 days, 3 mL of the sample provided for each subject was applied onto the entire face using an airbrush. The subjects were divided into a control group (C), an experimental group 1 (ADSC-CM 5% group, E1) and an experimental group 2 (polymersome-containing of ADSC-CM 5% group, E2), and there were 20 subjects in each group.ResultsMoisture content on the U-zone, melanin and erythema of lid-cheek, overall size and depth of eye wrinkles, and skin roughness of the items showed significant differences among all three groups. More specifically, E1 had a higher rate of skin improvement than C, and E2 had the highest skin improvement rate in all items: skin moisture content, transepidermal water loss (TEWL), melanin and erythema of lid-cheek, eye wrinkles, and skin roughness of lid-cheek.ConclusionsThe effectiveness of ADSC-CM was verified in all items: increases in moisture content, decrease in TEWL, and improvements of whitening and wrinkles. Moreover, the effectiveness of ADSC-CM was observed as higher in samples of polymersome-containing ADSC-CM than those of raw material ADSC-CM. Furthermore, percutaneous absorption was improved compared with previous studies on formulations in relation to polymersome at the same concentration of ADSC-CM, thereby resulting in higher effectiveness.Trial registrationKorea National Institute for Bioethics Policy, P01–201809–13-001, Registered 06 September 2018, http://public.irb.or.kr/.

Objective analysis of volume restoration in atrophic acne scars and skin pores: a split study using human stem cell-conditioned media

Background: It is anticipated that the combined treatments of ablative laser and human stem cell-conditioned media produce the synergistic effects.Objectives: To investigated the effects of human stem cell-conditioned media (HSCM) as a post-procedural agent after fractional CO2 laser procedure in patients with atrophic acne scars and skin pores. Method: Both cheeks of 15 subjects were treated with a fractional CO2 laser. HSCM was randomly applied to one of the resurfacing sites (T) and normal saline was applied to the other site as a control (C). During the next six days, a solution containing 80% HSCM and hyaluronic acid (HA) was applied on the treated side (T) and HA alone was applied to the control side (C). Scar volume and erythema were objectively evaluated using an Antera 3D® CS. Result: After two months, the scar volume was reduced by 23.5% (T) versus 15.0% (C) (p = .143) and the volume of the skin pores was reduced by 37.6% (T) versus 15.9% (C) (p = .006), while the erythema was increased by 2.8% (T) versus 3.1% (C) (p = .934). Atrophic scar and the skin pores in the HSCM-applied area improved by at least 15.0% after a single treatment session, suggesting better results compared with the control side.Conclusion: HSCM may augment the regenerative effects of fractional CO2 laser.

Efficacy of microneedling plus human stem cell conditioned medium for skin rejuvenation: a randomized, controlled, blinded split-face study.

BackgroundThe use of growth factors in skin rejuvenation is emerging as a novel anti-aging treatment. While the role of growth factors in wound healing is well established, their use in skin rejuvenation has only recently been to be studied and no controlled trials have been performed.ObjectiveWe evaluated the anti-aging effects of secretory factors of endothelial precursor cells differentiated from human embryonic stem cells (hESC-EPC) in Asian skin.MethodsA total of 25 women were included in this randomized, controlled split-face study. The right and left sides of each participant's face were randomly allocated to hESC-EPC conditioned medium (CM) or saline. To enhance epidermal penetration, a 0.25-mm microneedle roller was used. Five treatment sessions were repeated at 2-week intervals.ResultsPhysician's global assessment of pigmentation and wrinkles after treatment revealed statistically significant effects of microneedling plus hESC-EPC CM compared to microneedling alone (p<0.05). Skin measurements by Mexameter and Visiometer also revealed statistically significant effects of microneedling plus hESC-EPC CM on both pigmentation and wrinkles (p<0.05). The only minimal adverse event was mild desquamation in one participant.ConclusionSecretory factors of hESC-EPC improve the signs of skin aging and could be a potential option for skin rejuvenation.