Human Pluripotent Mesenchymal Stem Cells Research Articles

Biological evaluation of aerosol–gel-derived hydroxyapatite coatings with human mesenchymal stem cells

The bioactive properties of hydroxyapatite (HAP) are evaluated for applications involving the enhancement of biocompatible prostheses by seeding human pluripotent mesenchymal stem cells (MSCs). The in vitro response of human MSCs seeded on aerosol–gel HAP coatings is addressed in this work. The processing of the HAP coatings has been carried out by the aerosol–gel technique using calcium nitrate and triethylphosphate as starting precursors. The characterization of the coatings was carried out by using transmission electron microscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy disperse X-ray microanalysis, and surface force microscopy, which confirmed the high performance of the HAP coatings. In vitro tests show that human MSCs adhere to aerosol–gel-derived HAP coatings and show proliferation signals on these surfaces.

Development of human mesenchymal stem cells on DC sputtered titanium nitride thin films.

The biocompatible properties of titanium nitride (TiN) have opened a new field of applications for this material. In the present work, TiN coatings with thicknesses around 1 microm have been prepared by DC magnetron sputtering. The aim has been to evaluate the adherence, growth and proliferation of human pluripotent mesenchymal stem cells (hMSCs) on the surface of TiN films with contrasted structural, electrical, and mechanical properties. For this purpose, the films were characterized by X-ray diffraction, scanning electron microscopy, sheet resistance measurements, and nanoindentation. Biological tests show that hMSCs adhere and proliferate onto TiN surfaces. The combination of the mechanical, electrical, and biological responses suggest that TiN coatings present appropriate properties to induce the in vitro stimulated differentiation of hMSCs. This possibility gives an added value to TiN based biomaterial coatings.