Cannibalism Research Articles

Protective effects of exercise on responses to combined social and environmental stress in prairie voles.

The combination of social and environmental stressors significantly influences psychological and physical health in males and females, and contributes to both depression and cardiovascular diseases. Animal models support these findings. Voluntary exercise may protect against some forms of stress; however, the protective effects of exercise against social stressors require further investigation. This study evaluated the influence of exercise on the impact of combined social and environmental stressors in socially monogamous prairie voles. Following a period of social isolation plus additional chronic environmental stress, prairie voles were either allowed access to a running wheel in a larger cage for 2 weeks or remained in sedentary conditions. A behavioral stress task was conducted prior to and following exercise or sedentary conditions. Heart rate (HR) and HR variability were evaluated after exercise or sedentary conditions. Group-based analyses indicated that exercise prevented elevated resting HR and promoted autonomic control of the heart. Exercise was also effective against social and environmental stress-induced forced swim test immobility. Some minor sex differences in behavior were observed in response to exercise intensity. This research informs our understanding of the protective influence of physical exercise against social and environmental stressors in male and female humans.

Exposing 24-hour cycles in bile acids of male humans

Bile acids are trans-genomic molecules arising from the concerted metabolism of the human host and the intestinal microbiota and are important for digestion, energy homeostasis and metabolic regulation. While diurnal variation has been demonstrated in the enterohepatic circulation and the gut microbiota, existing human data are poorly resolved, and the influence of the host circadian system has not been determined. Using entrained laboratory protocols, we demonstrate robust daily rhythms in the circulating bile acid pool in healthy male participants. We identify temporal relationships between bile acids and plasma lipids and show that these relationships are lost following sleep deprivation. We also highlight that bile acid rhythmicity is predominantly lost when environmental timing cues are held constant. Here we show that the environment is a stronger determinant of these temporal dynamics than the intrinsic circadian system of the host. This has significance for the intimate relationship between circadian timing and metabolism.

Muscularity bias internalization moderates the associations of muscularity dissatisfaction with muscularity-oriented disordered eating and psychosocial well-being in men but not women

Prior research demonstrates positive associations between muscularity dissatisfaction and an array of negative health outcomes, including muscularity-oriented disordered eating, eating-related psychosocial impairment, and psychological distress. However, muscularity dissatisfaction differs by gender and is not always linked to these outcomes, indicating the existence of moderators of these associations. We proposed and examined muscularity bias internalization (MBI) as a moderator of these associations. The study included online samples of 450 Chinese men and 450 Chinese women who completed measures assessing MBI, muscularity dissatisfaction, muscularity-oriented disordered eating, eating-related psychosocial impairment, and psychological distress. Pearson correlation analyses examined the bivariate associations among study variables. Multiple linear regression analyses examined the moderating effects of MBI. Results revealed that both muscularity dissatisfaction and MBI were significantly and positively related to all outcome variables, except for a non-significant correlation between muscularity dissatisfaction and psychological distress in women. MBI was a significant moderator in men, not women, with muscularity dissatisfaction being more strongly and positively related to all outcome variables in men reporting higher MBI. Our study provides preliminary evidence supporting gender differences in the role of MBI in the associations of muscularity dissatisfaction with muscularity-oriented eating disorder psychopathology and psychosocial well-being.

Ejaculate Adjustment in Response to Sperm Competition Risk in Humans.

Previous research suggests that human males, like males of many mammalian and avian species, adjust their ejaculate quality in accordance with sperm competition risk. Men who spend less time with their regular female partner since the couple's last copulation produce ejaculates with more sperm at the couple's next copulation (Baker & Bellis, 1993). We conducted a conceptual replication of this research to investigate whether sperm competition risk predicts ejaculate adjustment in human males using additional measures of sperm competition risk (e.g., perceptions of partner infidelity, presence of potential sexual rivals) and updated laser-optic semen analysis technology. We collected data from 34 heterosexual couples (age range 18-32years) from a university population who completed self-report surveys on their relationship dynamics and provided six ejaculate samples (three copulatory and three masturbatory) across a 45-day period. Time spent together since the couple's last copulation was not significantly associated with ejaculate quality. However, sperm concentration for copulatory ejaculates was higher for men who perceived more potential sexual rivals. Discussion situates the current results within the literature on human sperm competition and suggests several directions for future research.

Day-to-day associations between testosterone, sexual desire and courtship efforts in young men.

Testosterone plays important roles in reproductive behaviour in many species. Despite a common belief that testosterone regulates fluctuations in human sexual desire, there is little direct evidence that relates within-person changes in natural testosterone production to within-person changes in sexual desire. Here, we measured daily salivary testosterone concentrations from 41 adult men for one month, along with daily self-reports of sexual desire (n = 759 observations for the main analyses). We analysed concurrent relationships between within-person changes in testosterone and desire, and also lagged relationships that were analysed using a continuous-time modelling framework. We found no evidence for significant, positive relationships between testosterone and desire, which argues against the notion that day-to-day changes in eugonadal men's baseline testosterone regulates changes in their sexual desire. However, additional analyses provided preliminary evidence for a positive relationship between testosterone and self-reported courtship effort, particularly on days when single participants interacted with potential romantic partners. Our findings add original evidence regarding day-to-day associations between testosterone and desire, and suggest that testosterone above minimum threshold concentrations does not increase sexual desire. We propose that the evolved functions of testosterone in human males are more closely associated with courtship efforts than with sexual desire.

Human-specific protein-coding and lncRNA genes cast sex-biased genes in the brain and their relationships with brain diseases

BackgroundGene expression shows sex bias in the brain as it does in other organs. Since female and male humans exhibit noticeable differences in emotions, logical thinking, movement, spatial orientation, and even the incidence of neurological disorders, sex biases in the brain are especially interesting, but how they are determined, whether they are conserved or lineage specific, and what the consequences of the biases are, remain poorly explored and understood.MethodsBased on RNA-seq datasets from 16 and 14 brain regions in humans and macaques across developmental periods and from patients with brain diseases, we used linear mixed models (LMMs) to differentiate variations in gene expression caused by factors of interest and confounding factors and identify four types of sex-biased genes. Effect size and confidence in each effect were measured upon the local false sign rate (LFSR). We utilized the biomaRt R package to acquire orthologous genes in humans and macaques from the BioMart Ensembl website. Transcriptional regulation of sex-biased genes by sex hormones and lncRNAs were analyzed using the CellOracle, GENIE3, and Longtarget programs. Sex-biased genes’ functions were revealed by gene set enrichment analysis using multiple methods.ResultsLineage-specific sex-biased genes greatly determine the distinct sex biases in human and macaque brains. In humans, those encoding proteins contribute directly to immune-related functions, and those encoding lncRNAs intensively regulate the expression of other sex-biased genes, especially genes with immune-related functions. The identified sex-specific differentially expressed genes (ssDEGs) upon gene expression in disease and normal samples also indicate that protein-coding ssDEGs are conserved in humans and macaques but that lncRNA ssDEGs are not conserved. The results answer the above questions, reveal an intrinsic relationship between sex biases in the brain and sex-biased susceptibility to brain diseases, and will help researchers investigate human- and sex-specific ncRNA targets for brain diseases.ConclusionsHuman-specific genes greatly cast sex-biased genes in the brain and their relationships with brain diseases, with protein-coding genes contributing to immune response related functions and lncRNA genes critically regulating sex-biased genes. The high proportions of lineage-specific lncRNAs in mammalian genomes indicate that sex biases may have evolved rapidly in not only the brain but also other organs.

Patterns of overeating in middle-aged Chinese from Hong Kong

Abstract Background Obesity is one of the most important risk factors for non-communicable diseases worldwide. Overweightness may be attributable to a wide spectrum of overeating behaviours. However, the various types of overeating behaviors are under-studied in Asia, particularly in non-adolescent age groups. This study aims to estimate the prevalence and factors associated with various overeating patterns among middle-aged Chinese adults. Methods A random telephone survey was conducted in 2023 on Hong Kong Chinese adults between 35 and 59 years or age (n = 785). An instrument composed of modified Positive-Negative Emotional Eating (EE) Scale, Night Eating Questionnaire-Chinese (NEQ), Binge Eating Disorder Screener (BED-7), Depression Anxiety and Stress Scales (DASS-21), demographic factors, BMI and number of reported gastrointestinal (GI) conditions was used. Multiple regression analyses were conducted to test the association of demographic factors, BMI, GI conditions as well as dysphoric mood with overeating behaviors. Results Hong Kong Chinese middle-aged adults reported the following past-month prevalence of overeating behaviors: positive EE (26.1%), general overeating (12.4%), negative EE (9.2%), binge eating (6.0%) and night eating behavior (1.8%). Nightshift work, mental stress, being single and younger age were common risk factors for the various overeating patterns among middle-aged men. Anxiety, high/low educational levels and younger age were common risk factors among middle-aged women. Obesity was associated with binge eating and positive EE in men (OR: 1.7-4.0) and associated with negative EE and general overeating (OR:2.3-2.7) in women. Conclusions This study noted that binge eating, night eating, positive/negative EE, and GOB were distinct eating patterns requiring different clinical approaches. The significant association between various overeating behaviors with BMI, indicates that these individuals should be prioritized to receive healthy eating interventions. Key messages • Adult overeating is not a singular behavior but represents an array of different eating patterns with different risk factors. • Like their Western counterparts, overeating behaviors in Chinese adults are often linked to dysphoric mood, therefore treatment of obesity may require psychological counseling.

Evolutionarily conserved fMRI network dynamics in the mouse, macaque, and human brain

Evolutionarily relevant networks have been previously described in several mammalian species using time-averaged analyses of fMRI time-series. However, fMRI network activity is highly dynamic and continually evolves over timescales of seconds. Whether the dynamic organization of resting-state fMRI network activity is conserved across mammalian species remains unclear. Using frame-wise clustering of fMRI time-series, we find that intrinsic fMRI network dynamics in awake male macaques and humans is characterized by recurrent transitions between a set of 4 dominant, neuroanatomically homologous fMRI coactivation modes (C-modes), three of which are also plausibly represented in the male rodent brain. Importantly, in all species C-modes exhibit species-invariant dynamic features, including preferred occurrence at specific phases of fMRI global signal fluctuations, and a state transition structure compatible with infraslow coupled oscillator dynamics. Moreover, dominant C-mode occurrence reconstitutes the static organization of the fMRI connectome in all species, and is predictive of ranking of corresponding fMRI connectivity gradients. These results reveal a set of species-invariant principles underlying the dynamic organization of fMRI networks in mammalian species, and offer novel opportunities to relate fMRI network findings across the phylogenetic tree.

Androgen signaling restricts glutaminolysis to drive sex-specific Th17 metabolism in allergic airway inflammation.

Females have an increased prevalence of many Th17 cell-mediated diseases, including asthma. Androgen signaling decreases Th17 cell-mediated airway inflammation, and Th17 cells rely on glutaminolysis. However, it remains unclear whether androgen receptor (AR) signaling modifies glutamine metabolism to suppress Th17 cell-mediated airway inflammation. We show that Th17 cells from male humans and mice had decreased glutaminolysis compared to females, and that AR signaling attenuated Th17 cell mitochondrial respiration and glutaminolysis in mice. Using allergen-induced airway inflammation mouse models, we determined females had a selective reliance upon glutaminolysis for Th17-mediated airway inflammation, and AR signaling attenuated glutamine uptake in CD4+ T cells by reducing expression of glutamine transporters. Minimal reliance on glutamine uptake in male Th17 cells compared to female Th17 cells was also found in circulating T cells from patients with asthma. AR signaling thus attenuates glutaminolysis, demonstrating sex-specific metabolic regulation of Th17 cells with implications for Th17 or glutaminolysis targeted therapeutics.

Early-life bullying victimization and perpetration and current disordered eating in Chinese men: An integrated theoretical model.

Early-life bullying victimization and perpetration and current disordered eating in Chinese men: An integrated theoretical model.

Heterogeneity of synaptic NMDA receptor responses within individual lamina I pain-processing neurons across sex in rats and humans.

Excitatory glutamatergic NMDA receptors (NMDARs) are key regulators of spinal pain processing, and yet the biophysical properties of NMDARs in dorsal horn nociceptive neurons remain poorly understood. Despite the clinical implications, it is unknown whether the molecular and functional properties of synaptic NMDAR responses are conserved between males and females or translate from rodents to humans. To address these translational gaps, we systematically compared individual and averaged excitatory synaptic responses from lamina I pain-processing neurons of adult Sprague-Dawley rats and human organ donors, including both sexes. By combining patch-clamp recordings of outward miniature excitatory postsynaptic currents with non-biased data analyses, we uncovered a wide range of decay constants of excitatory synaptic events within individual lamina I neurons. Decay constants of synaptic responses were distributed in a continuum from 1-20ms to greater than 1000ms, suggesting that individual lamina I neurons contain AMPA receptor (AMPAR)-only as well as GluN2A-, GluN2B- and GluN2D-NMDAR-dominated synaptic events. This intraneuronal heterogeneity in AMPAR- and NMDAR-mediated decay kinetics was observed across sex and species. However, we discovered an increased relative contribution of GluN2A-dominated NMDAR responses at human lamina I synapses compared with rodent synapses, suggesting a species difference relevant to NMDAR subunit-targeting therapeutic approaches. The conserved heterogeneity in decay rates of excitatory synaptic events within individual lamina I pain-processing neurons may enable synapse-specific forms of plasticity and sensory integration within dorsal horn nociceptive networks. KEY POINTS: Synaptic NMDA receptors (NMDARs) in spinal dorsal horn nociceptive neurons are key regulators of pain processing, but it is unknown whether their functional properties are conserved between males and females or translate from rodents to humans. In this study, we compared individual excitatory synaptic responses from lamina I pain-processing neurons of male and female adult Sprague-Dawley rats and human organ donors. Individual lamina I neurons from male and female rats and humans contain AMPA receptor-only as well as GluN2A, GluN2B- and GluN2D-NMDAR-dominated synaptic events. This may enable synapse-specific forms of plasticity and sensory integration within dorsal horn nociceptive networks. Human lamina I synapses have an increased relative contribution of GluN2A-dominated NMDAR responses compared with rodent synapses. These results uncover a species difference relevant to NMDAR subunit-targeting therapeutic approaches.

Metabolic benefits afforded by estradiol and testosterone in both sexes: clinical considerations.

Testosterone (T) and 17β-estradiol (E2) are produced in male and female humans and are potent metabolic regulators in both sexes. When E2 and T production stops or decreases during aging, metabolic dysfunction develops and promotes degenerative metabolic and vascular disease. Here, we discuss the shared benefits afforded by E2 and T for metabolic function human females and males. In females, E2 is central to bone and vascular health, subcutaneous adipose tissue distribution, skeletal muscle insulin sensitivity, antiinflammatory immune function, and mitochondrial health. However, T also plays a role in female skeletal, vascular, and metabolic health. In males, T's conversion to E2 is fundamental to bone and vascular health, as well as prevention of excess visceral adiposity and the promotion of insulin sensitivity via activation of the estrogen receptors. However, T and its metabolite dihydrotestosterone also prevent excess visceral adiposity and promote skeletal muscle growth and insulin sensitivity via activation of the androgen receptor. In conclusion, T and E2 are produced in both sexes at sex-specific concentrations and provide similar and potent metabolic benefits. Optimizing levels of both hormones may be beneficial to protect patients from cardiometabolic disease and frailty during aging, which requires further study.

Sexual Dimorphism in the Architectural Design of Rat and Human Pelvic Floor Muscles.

Skeletal muscle architecture is a strong predictor of in vivo functional capacity and is evaluated in fixed tissues, accommodating the study of human muscles from cadaveric donors. Previous studies evaluating the pelvic floor muscles (PFMs) demonstrated that the rat is the most appropriate small animal model for the study of female PFM architecture, but the rat's suitability for the study of male PFMs is undetermined. We aimed to determine (1) whether PFM architecture exhibits sexual dimorphism in rats or humans, and (2) if the rat is also a suitable animal model for the study of male human PFMs. PFMs were fixed in situ and harvested en bloc from male and female cadaveric donors and 3-month-old male and female Sprague-Dawley rats. Three architectural parameters influenced by species size were used to compare male versus female PFMs within species, while four size-independent measures compared species within sex. All comparisons were made with two-way analysis of variances and Tukey's multiple comparisons tests post hoc. Sarcomere length (rats and humans, p = 0.016 and = 0.002) and normalized fiber length (rats, p < 0.001) were significantly larger in male PFMs. Three of the size-independent measures exhibited similar species trends in both sexes, while the size-independent sarcomere length measure (Ls/Lso) differed between male rats and humans (p < 0.001). Thus, sexual dimorphism is present in rat and human PFM architecture, and the male rat is suitable for studies of human male PFMs.

Longitudinal proteomics of leptin treatment in humans with acute and chronic energy deficiency-induced hypoleptinemia reveal novel, mainly immune-related, pleiotropic effects

BackgroundLeptin is known for its metabolic, immunomodulatory and neuroendocrine properties, but the full spectrum of molecules downstream of leptin and relevant underlying mechanisms remain to be fully clarified. Our objective was to identify proteins and pathways influenced by leptin through untargeted proteomics in two clinical trials involving leptin administration in lean individuals. MethodsWe performed untargeted liquid chromatography-tandem mass spectrometry serum proteomics across two studies a) Short-term randomized controlled crossover study of lean male and female humans undergoing a 72-h fast with concurrent administration of either placebo or high-dose leptin; b) Long-term (36-week) randomized controlled trial of leptin replacement therapy in human females with acquired relative energy deficiency and hypoleptinemia. We explored longitudinal proteomic changes and run adjusted mixed models followed by post-hoc tests. We further attempted to identify ontological pathways modulated during each experimental condition and/or comparison, through integrated qualitative pathway and enrichment analyses. We also explored dynamic longitudinal relationships between the circulating proteome with clinical and hormonal outcomes. Results289 and 357 unique proteins were identified per each respective study. Short-term leptin administration during fasting markedly upregulated several proinflammatory molecules, notably C-reactive protein (CRP) and cluster of differentiation (CD) 14, and downregulated lecithin cholesterol acyltransferase and several immunoglobulin variable chains, in contrast with placebo, which produced minimal changes. Quantitative pathway enrichment further indicated an upregulation of the acute phase response and downregulation of immunoglobulin- and B cell-mediated immunity by leptin. These changes were independent of participants' biological sex. In the long term study, leptin likewise robustly and persistently upregulated proteins of the acute phase response, and downregulated immunoglobulin-mediated immunity. Leptin also significantly and differentially affected a wide array of proteins related to immune function, defense response, coagulation, and inflammation compared with placebo. These changes were more notable at the 24-week visit, coinciding with the highest measured levels of serum leptin. We further identified distinct co-regulated clusters of proteins and clinical features during leptin administration indicating robust longitudinal correlations between the regulation of immunoglobulins, immune-related molecules, serpins (including cortisol and thyroxine-binding globulins), lipid transport molecules and growth factors, in contrast with placebo, which did not produce similar associations. ConclusionsThese high-throughput longitudinal results provide unique functional insights into leptin physiology, and pave the way for affinity-based proteomic analyses measuring several thousands of molecules, that will confirm these data and may fully delineate underlying mechanisms.

The relationship between semen quality in male infertility clinic patients and bisphenol A：A Chinese cross-sectional study

Bisphenol A (BPA) is a growing concern as an endocrine-disrupting chemical due to its adverse health effects. However, the association between BPA and sperm quality in adult human males remains unclear. The aim of this study was to assess the daily life exposure level of BPA and analyze its correlation with sperm quality in males. Patients who sought treatment in Chinese infertility clinics between May and October 2023 were selected as study subjects. We determined participants' serum BPA content using high-performance liquid chromatography. Sperm count and motility were assessed using a computer-aided sperm analysis system, while sperm morphology was analyzed using an improved Papanicolaou stain. A total of 405 participants, averaging 33.01 ± 5.44 years old, were included. We observed low semen quality among participants in infertility clinics. Principal component analysis was performed for each semen quality index, and three principal components reflecting sperm motility, count, and morphology were extracted. The participants' mean serum BPA level was 6.96 ng/mL. Negative correlations were observed between serum BPA content and total sperm count, sperm density, forward motility rate, and non-forward motility rate. A positive correlation was found between the non-motile sperm rate and the head deformity rate. Morphological abnormalities were the predominant adverse effects observed. Despite low daily life BPA exposure, long-term low-dose exposure in the general population may damage semen quality. This study provides a scientific basis for managing health risks associated with BPA exposure.

Single nucleotide polymorphisms in protamine 2 genes in fertile and infertile human males in Southwest, Nigeria

Single nucleotide polymorphisms in protamine 2 genes in fertile and infertile human males in Southwest, Nigeria

Human risk assessment through development and application of a physiologically based toxicokinetic model for 4-tert-octylphenol

4-tert-octylphenol (4-tert-OP) is an ecologically hazardous substance, and exposure to it in the environment has been consistently reported in the past. Despite the hazards and widespread exposure to 4-tert-OP, tools for scientific assessment of 4-tert-OP exposure risk level in humans are lacking. The main purpose of this study was to develop a physiologically-based-toxicokinetic (PBTK) model for 4-tert-OP and to perform quantitative risk assessment of 4-tert-OP in various population groups using the established model. Based on the results of toxicokinetic experiments on male rats, the PBTK model for 4-tert-OP was established and verified, and this was converted to a model for humans through interspecies extrapolation. Based on the previously reported no-observed-adverse-effect-levels for rats, it was possible to estimate the 4-tert-OP reference dose in humans through reverse dosimetry using the model. Biomonitoring data derived from various population groups were applied to the human PBTK model to calculate external exposures and margin of safety for 4-tert-OP for each population group. The PBTK model established in this study adequately explained the toxicokinetic experimental values at acceptable levels and was able to quantitatively predict the 4-tert-OP exposure level in the testes related to male reproductive toxicity. In addition, the degree of external exposure to 4-tert-OP could be scientifically estimated based on biomonitoring values derived from various biological matrices. The reference doses for systemic and reproductive toxicity caused by 4-tert-OP in male humans were calculated to be 0.16 and 1.12 mg/kg/day, respectively. The mean external exposure to 4-tert-OP in each population group estimated based on plasma and urine biomonitoring data was 0.04–66.24 mg/kg/day, showing very large exposure diversity between groups. Exposure risks to 4-tert-OP in populations ranged from safe to risky, suggesting the need for continued monitoring and risk management of 4-tert-OP worldwide. This study provides valuable scientific insight regarding the 4-tert-OP human risk assessment.

A Call for More Culture-Based Research to Understand Body Dissatisfaction and Disordered Eating in Men: Commentary on Monocello et al. (2024).

Using culture-based approaches, Monocello et al. revealed that young Korean men generally shared the same perceptions of fatness, including three categories ("too thin," "balanced," and "too fat"). The "balanced" category was most consistent with local South Korean culture, and the "too fat" category was associated with higher body dissatisfaction and disordered eating. Even though cultural differences in body ideals are an essential factor to consider in the development of body dissatisfaction and disordered eating, little research has applied culture-based approaches to explore body ideals in men, including how these ideals may be related to men's body image or eating behaviors. Thus, Monocello et al.'s work is a timely and vital contribution to the literature. In this commentary, we expand on Monocello et al.'s work by not only illustrating the roles of local cultures but also introducing the potential influences of external cultures via acculturation in shaping body ideals which, in turn, contribute to body dissatisfaction and disordered eating in men. We also provide future research directions to call for more culture-based research to understand body dissatisfaction and disordered eating among men in different cultural contexts.

Evaluating the effect of ashwagandharishta on human respiratory system by utilizing spirometry

The key objective of this present study is to analyze the effect(s) of Ashwagandharishta on the lung function of human males. After completing the 45 days of study, the parameter FVC decreased by 3.315 % than the initial period of the study of the control group. On the other hand, the parameters FEV1, FVFC, PEF, FEF25–75%, MEF 75%, MEF 50% and MEF 25% level was increased by 0.2951 %, 5.600 %, 0.971 %, 4.137 %, 1.909 %, 4.590 %, 5.777 %, 7.182 % and 10.635 % respectively after the 45 days of study than the initial period of the control group. Here no result was statistically significant. After the Chronic administration of Ashwagandharishta (ASG) to human male subjects for 45 days, the values of the parameters FVC, FEV1, FVFC, PEF, FEF25–75%, MEF 75% and MEF 50% was decreased by 4.109 %, 5.351 %, 1.077 %, 16.042 %, 7.199 % and 21.074 % respectively than the values of the initial period of the study of the ASG treated group. On the other hand, the parameter MEF 25% level was increased by 2.202 % after the 45 days chronic administration of the ASG. No result was statistically significant. Jahangirnagar University J. Biol. Sci. 12(1 &amp; 2): 45-53, 2023 (June &amp; December)

Common and unique neurophysiological signatures for the stopping and revising of actions reveal the temporal dynamics of inhibitory control.

Inhibitory control is a crucial cognitive-control ability for behavioral flexibility that has been extensively investigated through action-stopping tasks. Multiple neurophysiological features have been proposed to represent 'signatures' of inhibitory control during action-stopping, though the processes signified by these signatures are still controversially discussed. The present study aimed to disentangle these processes by comparing simple stopping situations with those in which additional action revisions were needed. Three experiments in female and male humans were performed to characterize the neurophysiological dynamics involved in action-stopping and - changing, with hypotheses derived from recently developed two-stage 'pause-then-cancel' models of inhibitory control. Both stopping and revising an action triggered an early broad 'pause'-process, marked by frontal EEG β-bursts and non-selective suppression of corticospinal excitability. However, partial-EMG responses showed that motor activity was only partially inhibited by this 'pause', and that this activity can be further modulated during action-revision. In line with two-stage models of inhibitory control, subsequent frontocentral EEG activity after this initial 'pause' selectively scaled depending on the required action revisions, with more activity observed for more complex revisions. This demonstrates the presence of a selective, effector-specific 'retune' phase as the second process involved in action-stopping and -revision. Together, these findings show that inhibitory control is implemented over an extended period of time and in at least two phases. We are further able to align the most commonly proposed neurophysiological signatures to these phases and show that they are differentially modulated by the complexity of action-revision.