Human Cultures Research Articles

Controlled cell proliferation and immortalization of human dental pulp stem cells with a doxycycline-inducible expression system.

Human dental pulp stem cells are a potentially useful resource for cell-based therapies and tissue repair in dental and medical applications. However, the primary culture of isolated dental pulp stem cells has notably been limited. A major requirement of an ideal human dental pulp stem cell culture system is the preservation of efficient proliferation and innate stemness over prolonged passaging, while also ensuring ease of handling through standard, user-friendly culture methods. In this study, we have engineered a novel human dental pulp stem cell line, distinguished by the constitutive expression of telomerase reverse transcriptase (TERT), and the conditional expression of the R24C mutant cyclin-dependent kinase 4 (CDK4R24C) and Cyclin D1. We have named this cell line Tet-off K4DT hDPSCs. Furthermore, we have conducted a comprehensive comparative analysis of their biological attributes in relation to a previously immortalized human dental pulp stem cells, hDPSC-K4DT, which were immortalized by the constitutive expression of CDK4R24C, Cyclin D1 and TERT. In Tet-off K4DT cells, the expression of the K4D genes can be precisely suppressed by the inclusion of doxycycline. Remarkably, Tet-off K4DT cells demonstrated an extended cellular lifespan, increased proliferative capacity, and enhanced osteogenic differentiation potential when compared to K4DT cells. Moreover, Tet-off K4DT cells had no observable genomic aberrations and also displayed a sustained expression of stem cell markers even at relatively advanced passages. Taken together, the establishment of this new cell line holds immense promise as powerful experimental tool for both fundamental and applied research involving dental pulp stem cells.

Lyric, lovers, and limitlessness: Shakespeare’s quarrel with time in Sonnet 55

As a genre, lyric remains difficult to define. Paul Celan perhaps best summarizes its meaning by referring to an encounter that lyric effects. In Sonnet 55, Shakespeare tests the limits of lyric against the most powerful of forces—time—showing that it can withstand its assaults better than stone monuments can preserve the king’s image. Shakespeare takes it one step further, however, hinting at the resurrective power of lyric and at the possibility of lyric’s lasting into eternity. Ultimately, Shakespeare succeeds in giving us an image of lyric as fostering human culture, birthing lovers and civilizations and outlasting both.

The influence of renewable energy, humanistic culture, and green knowledge on corporate social responsibility and corporate environmental performance

The influence of renewable energy, humanistic culture, and green knowledge on corporate social responsibility and corporate environmental performance

Comparing the nutritional composition and antioxidant properties of an Australian native grain variety with commonly consumed wheat

SummaryClimate change is threatening our current food systems and countries around the world are seeking alternative food sources to safeguard against potential food insecurity and nutrient deficiency concerns. Various traditional foods used by Aboriginal groups of Australia have previously been shown to be highly nutritious, contain beneficial functional properties and can thrive in the harsh climatic conditions found across Australia. As interest in these traditional foods grows, Aboriginal groups are looking at developing a range of foods for modern markets. However, for many of these foods, there is still little known about their nutritional and functional properties. In this study, we report the nutrition composition and antioxidant properties of a native Australian grain species that is currently being developed for commercial markets by a collective of Aboriginal Traditional Custodians and compare its properties to a commonly consumed wheat variety. Compared with wheat, the native grain species contained 2‐fold greater protein and total fats, and higher levels of essential minerals and trace elements, including 8‐fold iron levels and >2.5‐fold calcium, magnesium, zinc, copper and manganese levels. Functionally, the native grain contained 2.4‐fold greater polyphenol content and displayed greater antioxidant potential in exposed human monocyte cultures. Importantly, the native grain was also found to contain a very low gluten content. Altogether, this shows that the native grain is nutritionally and functionally superior to commonly consumed wheat for the measured parameters and could potentially serve as a grain alternative or be used to fortify current wheat‐based and gluten‐free products.

#1502 Short-term effects of gadolinium and gadoteric acid on kidney — impact on inflammation

Abstract Background and Aims The nephrotoxicity of free gadolinium [Gd (III)] has been reported, raising concerns about the safety of gadolinium-based contrast agents (GBCA), widely used in magnetic resonance imaging. The GBCA with macrocyclic structure, such as gadoteric acid (Gd-DOTA), appear as more stable. Using human proximal tubular cells cultures, we found that free Gd (III) triggers death by apoptosis and necrosis, and increases the expression of modulators of inflammation, hypoxia and fibrosis, which are known as major risk factors for the development and worsening of kidney disease. Our aim was, using animal models, to evaluate the short-term effects of Gd (III) and of gadoteric acid on inflammatory biomarkers, at blood and renal tissue levels. Method Wistar rats were exposed to a single dose of Gd (III) or Gd-DOTA (groups A and B, respectively); a control group was also included (C). After 48h, blood and kidney samples were collected. The plasma levels of interleukin (IL)6 and transforming growth factor (TGF) beta 1 were assessed by ELISA; the kidney gene expression of IL6 (il6), TGF beta 1 (tgbf1) and nuclear factor-kappa B (nfkb1) was determined through qPCR. Results Gd (III) group (A) presented higher circulating IL6 levels (P = 0.001) and increased kidney gene expression of il6 (P = 0.013), tgbf1 (P = 0.006) and nfkb1 (P = 0.011) than the control group (C); compared to Gd-DOTA group (B), the IL6 plasma concentration (P = 0.005) and tgbf1 mRNA levels were also higher (P = 0.004). The Gd-DOTA group (B) presented increased gene expression of il6 (P = 0.006) than the control group (C). Conclusion Short-term exposition to free Gd (III) induced an inflammatory response, shown by the increase in circulating IL6, and in kidney gene expression of il6, tgbf1 and nfkb1. The exposition to Gd-DOTA was associated with a lower inflammatory response, showing only increased kidney gene expression of il6. Despite the much safer profile for Gd-DOTA, further studies are warranted, evaluating other biomarkers, to clarify the short-term, and even the long-term effects, of these compounds. Acknowledgements This work was financed by FCT through the project 2022.08400.PTDC; FCT, in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences—UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB.

Способы мифотворчества и их специфика (на примере художественных и публицистических текстов Умберто Эко)

The study aims to identify the specifics of myth-making techniques that are comprehended and tested by the 20th- and 21st-century European philosopher Umberto Eco in his numerous publicistic and literary texts. As a result of the study, classifications of the “traditional” ways of myth-making (the reference to traditional mythologized archetypes, the use of binary oppositions, etc.) and the “modern” ways (double coding, depersonalization, discreteness of history, etc.), determined by the cultural and historical context of postmodernism and post-structuralism were provided. Using these classifications, the significance and influence of myth and myth-making techniques on thinking, human behavior, and cultural and historical processes of past and modern eras were proven. The paper is novel in that by analyzing the complex of Umberto Eco’s texts, myth is considered as the most important construct both of human culture and human thinking. The mechanisms of constructing certain types of modern myths, as well as the ways of their influence on the mass consciousness, on historical and socio-cultural processes are described.

Electrospun fibers modified with polydopamine for enhancing human mesenchymal stem cell culture

In this study, we coated electrospun polycaprolactone (PCL) fibers with polydopamine (PDA) to modify their hydrophobicity and fabricated a matrix for culturing mesenchymal stem cells (MSCs). Additionally, we incorporated Arg-Gly-Asp (RGD) peptides into PDA to enhance MSCs culture performance on PCL fibers. PDA and RGD were successfully coated in one step by immersing the electrospun fibers in a coating solution, without requiring an additional surface activation process. The characteristics of functionalized PCL fibers were analyzed by scanning electron microscopy with energy-dispersive x-ray analysis, Fourier transform infrared spectroscopy, water contact angle measurement, and fluorescence measurements using a carboxylic-modified fluorescent microsphere. MSCs cultured on the modified PCL fibers demonstrated enhanced cell adhesion, proliferation, and osteogenic- and chondrogenic differentiation. This study provides insight into potential applications for scaffold fabrication in MSCs-based tissue engineering, wound dressing, implantation, and a deeper understanding of MSCs behavior in vitro.

Spread of Ideas: The Legacy of Diffusionism School

This comprehensive analysis explores the concept and development of diffusionism throughout the history of anthropology. Diffusionism, a theory that emerged in the early 20th century, posits that cultural elements are assimilated and disseminated throughout multiple nations, leading to transformative shifts in society. The German and British diffusionist schools were the primary institutions that concentrated on the transmission of cultural characteristics across temporal and spatial dimensions. The notion of "culture circles," first proposed by German diffusionism, led by figures like Wilhelm Schmidt and Robert Fritz Craebner, posited that the cultural traits of ancient civilizations spread to the surrounding regions. British diffusionism, spearheaded by Grafton Elliot Smith and V. J. Perry, emphasized Egypt as the birthplace of civilization and attributed cultural advancements to Egyptian origins. Franz Boas, the founder of the US diffusionist school, advocated for a historical-cultural perspective that opposed the idea of linear progress. Boas emphasized the importance of culture as a unified entity shaped by particular historical circumstances and dismissed notions of cultural advancement. Notable American diffusionists include J. Robert Lowie, Edward Sapir, Clark Wissler, and Alfred Louis Kroeber. Kroeber's examination of women's fashion trends and cultural zeniths demonstrates the multifaceted nature of diffusionist analysis. Lowie's study focused on challenging linear evolution ideas and emphasizing the crucial significance of spatial dispersion in understanding cultural events. Wissler concentrated on the biological components of disperse on, whereas Sapir examined how individual personalities shape cultural patterns. While diffusionism is not as common in modern anthropology, it is important for comprehending the intricacies of cultural transmission and societal progress. Diffusionism provides valuable insights into the dynamics of human culture and civilization by examining historical processes, geographic distribution, and cultural interaction.

Enhancing Organizational Resilience: The Transformative Influence of Strategic Human Resource Management Practices and Organizational Culture

In an era characterized by rapid changes and uncertainty, organizational resilience emerges as a critical factor for the survival and prosperity of businesses. Confronted with a progressively volatile and unpredictable organizational landscape, an organization’s capacity to swiftly and effectively address challenges and adapt to changes becomes indispensable. This paper investigates the role of strategic human resource management (SHRM) practices and organizational culture (OC) in enhancing organizational resilience (OR). The research employed a questionnaire distributed to 501 employees within public institutions, with data analysis conducted using structural equation modeling. The study assesses the direct effects of SHRM practices on organizational resilience while also investigating the mediating effects of organizational culture on the relationship between these practices and resilience. The primary findings highlight both the direct and indirect impacts of SHRM practices on organizational resilience. The study underscores the importance of adopting an integrated approach, wherein SHRM practices and organizational culture are strategically aligned to bolster organizational resilience and secure sustainable success in a dynamic and turbulent organizational landscape.

A youthful touch: Reversal of aging hallmarks by cell reprogramming.

With the elderly population projected to double by 2050, there is an urgent need to address the increasing prevalence of age-related debilitating diseases and ultimately minimize discrepancies between the rising lifespan and stagnant healthspan. Cellular reprogramming by over-expression of Oct3/4, Klf4, Sox2, and cMyc (OKSM) transcription factors is gaining attention in this context thanks to demonstrated rejuvenating effects in human cell cultures and live mice, many of which can be uncoupled from de-differentiation and loss of cell identity. Here, we review current evidence of the impact of cell reprogramming on established aging hallmarks and the underlying mechanisms that mediate these effects. We also provide a critical assessment of the challenges in translating these findings and, overall, cell reprogramming technologies into clinically translatable anti-aging interventions. cellular reprogramming has the potential to reverse at least partially some key hallmarks of aging. However, further research is necessary to determine the biological significance and duration of such changes, and to ensure the safety of cell reprogramming as a rejuvenation approach. With this review, we hope to stimulate new research directions in the quest to extend healthspan effectively.

Musical protein: Mapping the time sequence of music onto the spatial architecture of proteins

Background and objectiveMusic, the ubiquitous language across human cultures, is traditionally considered as a form of art but has been linked to biomolecules in recent years. However, previous efforts have only been addressed on sonification of nucleic acids and proteins to produce so-called life music, the soundscape from the basic building blocks of life. In this study, we attempted to, for the first time, conduct a reverse operation of this process, i.e. conversion of music to protein (CoMtP). MethodsA novel notion termed musical protein (MP) –– the protein defined by music –– was proposed and, on this basis, we described a computational strategy to map the time sequence of music onto the spatial architecture of proteins, which considered that each note in the stave of a music (target) can be simply characterized by two acoustical quantities and that each residue in the primary sequence of a protein (hit) was represented by amino acid descriptors. ResultsA simulated annealing (SA) algorithm was applied to iteratively generate the best matched MP hit for a music target and structural bioinformatics was then used to model spatial advanced structure for the resulting MP. We also demonstrated that some small MPs derived from music segments may have potential biological functions, which, for example, can serve as antimicrobial peptides (AMPs) to inhibit clinical bacterial strains with moderate or high antibacterial potency. ConclusionsThis work may benefit many aspects; for example, it would open a door for the hearing-impaired persons to ‘listen’ music in a biological vision and could be a mean of exposing students to the concepts of biomolecules at an earlier age through the use of auditory characteristics. The CoMtP would also facilitate the rational design of proteins with biological and medicinal significance.

PHYSICAL CULTURE IS A FUNDAMENTAL COMPONENT OF THE MENTAL AND PHYSICAL DEVELOPMENT OF MODERN YOUTH AND STUDENTS

Physical culture is a complex social phenomenon that is not limited to solving problems of physical development, but also performs other social functions of society in the field of morality, education, and ethics. It has no social, professional, biological, age, or geographical boundaries. The theory of physical culture proceeds from the basic principles of the theory of culture and is based on its concepts. At the same time, it has specific terms and concepts that reflect its essence, goals, objectives, content, as well as means, methods, and guidelines. The main and most general concept is “physical culture”. As a type of culture, it represents in general social terms is a vast area of creative activity to create people’s physical readiness for life (health promotion, development of physical abilities and motor skills). In personal terms, physical culture is a measure and method of comprehensive physical development of a person. Thus, physical culture is a type of culture that is a specific process and result of human activity, a means and method of physical improving a person to fulfill social responsibilities. There are other definitions of this concept, in particular: physical culture is a specific part universal human culture, one of the areas of social activity aimed at strengthening health, increasing its level, and the comprehensive development of physical human abilities and their use in social practice, in people’s everyday lives [4]. Physical culture is an important part of the culture of society - the totality of its achievements in its creation and rational use of special means, methods, and conditions for directed physical improvement of a person. In the content of physical culture itself, two main aspects can be distinguished with some convention: – firstly, everything valuable that society creates and uses as special means, methods and conditions for their use, allowing to optimize physical development and ensure a certain level of physical fitness of people (functionally supporting side of physical culture);– secondly, the positive results of using these means, methods and conditions (the effective side of physical culture).

The Interaction Between Cumulative Culture and Gender

In a society characterized by rapid technological and social advancements, we inherit knowledge from our predecessors while simultaneously building upon it to forge new cultural developments, and such process contributes to the Cumulative culture, the continuous and interactive process of adding innovations to a socially heritable unity of knowledge. Considering the prevalence of gender inequality background in our cultures, this academic article explores the intricate relationship between gender equality and cumulative culture. This paper posits that gender, as defined within the constraints of male and female due to various limitations, plays a pivotal role in this cumulative cultural process. As the results, the examination reveals a clear and positive association between cumulative culture and gender equality. Cumulative culture wields a significant influence over children's choices of gender role models, consequently impacting knowledge transmission and children's labor allocation. Simultaneously, fostering gender egalitarianism offers a pivotal avenue for advancing the evolution of human cumulative culture. This paper aims to declare the benefits of gender equality towards cultural evolution and humanity and proposes the potential ways to foster cultural societal thriving.

A concise review: edible mushroom and their medicinal significance

In many areas of human life, including food, health, culture, and religion, mushrooms have had a significant impact. Most people eat mushrooms for their flavor and texture. Recently, they have gained popularity as a protein source and a drug research tool. According to the phyla Ascomycota and Basidiomycota, mushrooms are fungi that produce spongy fruiting bodies, particularly those that possess a stalk and an envelope top. Mushrooms are composed of 90% water and 10% dry material. Additionally, it has a physicochemical composition that is important for nutrition. Edible mushrooms have been shown to offer therapeutic benefits, including anti-cancer, cardiovascular, hepatoprotective, neuroprotective, hypolipidemic, antiviral, antibacterial, and anti-diabetic actions. Mushrooms are a fantastic source of nourishment since they are rich in proteins, minerals, complex sugars, unsaturated fatty acids, and secondary metabolites. The composition and nutritional benefits of edible mushrooms have been carefully investigated in this review. Edible mushrooms have been used as potential therapeutic stand-ins, and bioactive components present in edible mushrooms, such as polyphenolic compounds and antioxidant activity, have also been studied. This review article may also help scientists, researchers, and medical professionals slow the advancement of some lifestyle diseases, neurological disorders, along autoimmune disorders.

Autonomous and Self-sustained Circadian Oscillators Displayed in Human Primary Parathyroid Cell Culture Drive Parathormone Secretion

Abstract Background Most cells in our body possess circadian oscillators controlling diurnal rhythmicity of body metabolism. The role of molecular clocks in regulating the function and disfunction of human parathyroid gland (PG), responsible for calcium homeostasis, has not been unraveled. Hyperparathyroidism is a common endocrine pathology, characterized by an unadapted PTH secretion to calcium levels. Aims Here we aimed at characterizing molecular makeup of circadian clocks in human primary parathyroid cell culture (HPPCC), and at establishing differential transcriptional patterns of normal and pathological PGs. Methods RNA extracted from normal, adenomatous, and hyperplastic PG tissues was subjected to RNA sequencing (RNAseq) analysis. The rim of normal PG tissue was dissected from parathyroid adenoma specimens based on the higher autofluorescence intensity assessed by the near infra-red imaging. HPPCCs were established from adenomatous and hyperplastic PG biopsies. Efficient circadian clock disruption was achieved in HPPCCs by small interfering RNA-mediated knockdown of CLOCK. The functionality of circadian clock machinery was assessed by continuous recording of circadian bioluminescence introduced via lentivectors and paralleled with around-the-clock measurement of PTH secretion using perfusion system. Results The RNAseq analyses revealed transcriptional signatures that distinguished between pathological and normal PGs, and among adenomatous and hyperplastic PGs. We report, for the first time, robust anti-phasic circadian rhythmicity of Per2-luciferase and Bmal1-luciferase reporters in HPPCCs synchronized in vitro. Strikingly, we observed circadian rhythmicity of PTH secretion by parathyroid adenoma cells. Moreover, clock disruption in PG adenoma cells has an impact on transcription of functional genes. Conclusion Our data indicate presence of cell-autonomous molecular clock in human PG cells paralleled with circadian rhythmic PTH secretion and provide large-scale transcriptional pattern of parathyroid adenoma and hyperplastic tissues.

Antiseizure properties of fenamate NSAIDs determined in mature human stem-cell derived neuroglial circuits

Repeated and uncontrolled seizures in epilepsy result in brain cell loss and neural inflammation. Current anticonvulsants primarily target ion channels and receptors implicated in seizure activity. Identification of neurotherapeutics that can inhibit epileptiform activity and reduce inflammation in the brain may offer significant benefits in the long-term management of epilepsy. Fenamates are unique because they are both non-steroidal anti-inflammatory drugs (NSAIDs) and highly subunit selective modulators of GABAA receptors. In the current study we have investigated the hypothesis that fenamates have antiseizure properties using mature human stem cell-derived neuro-glia cell cultures, maintained in long-term culture, and previously shown to be sensitive to first, second and third generation antiepileptics. Mefenamic acid, flufenamic acid, meclofenamic acid, niflumic acid, and tolfenamic acid (each tested at 10–100 μM) attenuated 4-aminopyridine (4-AP, 100 μM) evoked epileptiform activity in a dose-dependent fashion. These actions were as effective diazepam (3–30 μM) and up to 200 times more potent than phenobarbital (300–1,000 μM). The low (micromolar) concentrations of fenamates that inhibited 4-AP evoked epileptiform activity correspond to those reported to potentiate GABAA receptor function. In contrast, the fenamates had no effect on neural spike amplitudes, indicating that their antiseizure actions did not result from inhibition of sodium-channels. The antiseizure actions of fenamates were also not replicated by either of the two non-fenamate NSAIDs, ibuprofen (10–100 μM) or indomethacin (10–100 μM), indicating that inhibition of cyclooxygenases is not the mechanism through which fenamates have anticonvulsant properties. This study therefore shows for the first time, using functionally mature human stem cell-derived neuroglial circuits, that fenamate NSAIDs have powerful antiseizure actions independent of, and in addition to their well-established anti-inflammatory properties, suggesting these drugs may provide a novel insight and new approach to the treatment of epilepsy in the future.

Hypersensitive blood vessels in Clarkson disease.

Idiopathic systemic capillary leak syndrome (ISCLS) is a rare, recurrent condition with dramatically increased blood vessel permeability and, therefore, induction of systemic edema, which may lead to organ damage and death. In this issue of the JCI, Ablooglu et al. showed that ISCLS vessels were hypersensitive to agents known to increase vascular permeability, using human biopsies, cell culture, and mouse models. Several endothelium-specific proteins that regulate endothelial junctions were dysregulated and thereby compromised the vascular barrier. These findings suggest that endothelium-intrinsic dysregulation underlies hyperpermeability and implicate the cytoplasmic serine/threonine protein phosphatase 2A (PP2A) as a potential drug target for the treatment of ISCLS.

The Biological Impact of Some Phosphonic and Phosphinic Acid Derivatives on Human Osteosarcoma.

Osteosarcoma malignancy currently represents a major health problem; therefore, the need for new therapy approaches is of great interest. In this regard, the current study aims to evaluate the anti-neoplastic potential of a newly developed phosphinic acid derivative (2-carboxyethylphenylphosphinic acid) and, subsequently, to outline its pharmaco-toxicological profile by employing two different in vitro human cell cultures (keratinocytes-HaCaT-and osteosarcoma SAOS-2 cells), employing different techniques (MTT assay, cell morphology assessment, LDH assay, Hoechst staining and RT-PCR). Additionally, the results obtained are compared with three commercially available phosphorus-containing compounds (P1, P2, P3). The results recorded for the newly developed compound (P4) revealed good biocompatibility (cell viability of 77%) when concentrations up to 5 mM were used on HaCaT cells for 24 h. Also, the HaCaT cultures showed no significant morphological alterations or gene modulation, thus achieving a biosafety profile even superior to some of the commercial products tested herein. Moreover, in terms of anti-osteosarcoma activity, 2-carboxyethylphenylphosphinic acid expressed promising activity on SAOS-2 monolayers, the cells showing viability of only 55%, as well as apoptosis features and important gene expression modulation, especially Bid downregulation. Therefore, the newly developed compound should be considered a promising candidate for further in vitro and in vivo research related to osteosarcoma therapy.

A Literary and Interpretive analysis of Bihu Songs' Identity

A Literary and Interpretive analysis of Bihu Songs' Identity

Rural reality contradicts the ethnographic literature—a nationwide survey on folk beliefs and people's affection for the stork in Poland

BackgroundDue to early synanthropization and ecological and behavioural features, the White Stork Ciconia ciconia became the most cherished of European birds. Rooted in human culture, the species has been well studied; nevertheless, knowledge of people’s attitudes and stork-related folk beliefs remain descriptive. Here, we attempt to quantify these issues in the world's largest stronghold of the species, Poland, in the 1950s.MethodsThe study is based on recently discovered, original nationwide survey data from the 1958 International White Stork Census. These materials were gathered to assess the population size, but they also included issues belonging to the humanities. We have worked them up in a quantitative manner, which has resulted in an original approach rarely found in ethnological studies. We aim to propose an original typology of stork-related beliefs, their spread and regional diversity in Poland and the relationship with stork abundance.ResultsA sample of 2343 questionnaires revealed that affection towards storks was widespread (91.4% positive responses), more so in eastern Poland. The most frequent beliefs relate to respect for the stork (65%) and prophesies (24%), thereafter parental beliefs (7%) and stork biology (3%). Positive attitudes and the dissemination of beliefs increased with stork densities but were unrelated to the respondents’ sex. Utilitarian beliefs outweighed those prioritized in ethnographic studies (e.g. the stork’s human origins) or popular today (baby-bringing), and expressed the real concerns of country folk.ConclusionsThe discovery of long-lost data bordering on ethnology and nature conservation and their novel work-up highlights a realistic dimension of the human–nature relationship and provides a benchmark for further interdisciplinary research.