Human Compatibility Research Articles

Frequency of human leukocyte antigen compatibility and graft rejection in kidney transplant patients from living donors

Frequency of human leukocyte antigen compatibility and graft rejection in kidney transplant patients from living donors

Human leukocyte antigen compatibility and incidence of donor-specific antibodies in pediatric liver transplant recipients.

Antibody-mediated rejection following liver transplantation (LT) has been increasingly recognized, particularly with respect to the emergence of de novo donor-specific antibodies (DSAs) and their impact on graft longevity. While substantial evidence for adult populations exists, research focusing on pediatric LT outcomes remains limited. To investigate the prevalence of human leukocyte antigen (HLA) mismatches and DSA and evaluate their association with rejection episodes after pediatric LT. A cohort of pediatric LT recipients underwent HLA testing at Santa Casa de Porto Alegre, Brazil, between December 2013 and December 2023. Only patients who survived for > 30 days after LT with at least one DSA analysis were included. DSA classes I and II and cross-matches were analyzed. The presence of de novo DSA (dnDSA) was evaluated at least 3 months after LT using the Luminex® single antigen bead method, with a positive reaction threshold set at 1000 MFI. Rejection episodes were confirmed by liver biopsy. Overall, 67 transplanted children were analyzed; 61 received grafts from living donors, 85% of whom were related to recipients. Pre-transplant DSA (class I or II) was detected in 28.3% of patients, and dnDSA was detected in 48.4%. The median time to DSA detection after LT was 19.7 [interquartile range (IQR): 4.3-35.6] months. Biopsy-proven rejection occurred in 13 patients at follow-up, with C4d positivity observed in 5/13 Liver biopsies. The median time to rejection was 7.8 (IQR: 5.7-12.8) months. The presence of dnDSA was significantly associated with rejection (36% vs 3%, P < 0.001). The rejection-free survival rates at 12 and 24 months were 76% vs 100% and 58% vs 95% for patients with dnDSA anti-DQ vs those without, respectively. Our findings highlight the importance of incorporating DSA assessment into pre- and post-transplantation protocols for pediatric LT recipients. Future implications may include immunosuppression minimization strategies based on this analysis in pediatric LT recipients.

FLUID: a fluorescence-friendly lipid-compatible ultrafast clearing method.

Many clearing methods achieve high transparency by removing lipid components from tissues, which damages microstructure and limits their application in lipid research. As for methods which preserve lipid, it is difficult to balance transparency, fluorescence preservation and clearing speed. In this study, we propose a rapid water-based clearing method that is fluorescence-friendly and preserves lipid components. FLUID allows for preservation of endogenous fluorescence over 60 days. It shows negligible tissue distortion and is compatible with various types of fluorescent labeling and tissue staining methods. High quality imaging of human brain tissue and compatibility with pathological staining demonstrated the potential of our method for three-dimensional (3D) biopsy and clinical pathological diagnosis.

Highly compressible porous polyethyleneimine/chitosan composite aerogel with excellent mechanical properties as wide detection range sensors for human motion monitoring

AbstractBiomass three‐dimensional composite aerogels have garnered significant attention in the realm of wearable electronic skin owing to their favorable properties, including excellent human compatibility, environmentally benign degradability, and continuous porous architecture. However, conventional biomass aerogels suffer from inadequate mechanical flexibility, susceptibility to irreversible deformation under high compressive stress, and limited reusability, thereby constraining their applicability in sensing technologies. To address these limitations, this study presents the development of porous CCS/KH560/PEI/CNT‐COOH (CKPC) composite aerogels through a freeze–drying process. Chemical crosslinking was achieved using silane coupling agent (KH560) with carboxymethyl chitosan (CCS) and polyethyleneimine (PEI), while carboxylated carbon nanotubes (CNT‐COOH) were incorporated as conductive fillers. This approach successfully overcame the issues of poor mechanical properties, low elasticity, and unbalanced sensitivity‐sensing range trade‐off in chitosan‐based aerogel sensors. The results revealed that the porous CKPC aerogels exhibited a remarkable mechanical compressive strain of 86.3% while maintaining structural integrity post‐unloading. The CKPC composite aerogel‐based sensor demonstrated a high sensitivity of 42.9 within a wide strain range of 60%–76.3%, accompanied by a stable and repeatable electrical signal response across varying strains. The porous structure of the CKPC conductive aerogel sensor holds promising applications in human motion monitoring and flexible electronics.

RAbDesFlow: a novel workflow for computational recombinant antibody design for healthcare engineering.

Recombinant antibodies (rAbs) have emerged as a promising solution to tackle antigen specificity, enhancement of immunogenic potential and versatile functionalization to treat human diseases. The development of single chain variable fragments has helped accelerate treatment in cancers and viral infections, due to their favorable pharmacokinetics and human compatibility. However, designing rAbs is traditionally viewed as a genetic engineering problem, with phage display and cell free systems playing a major role in sequence selection for gene synthesis. The process of antibody engineering involves complex and time-consuming laboratory techniques, which demand substantial resources and expertise. The success rate of obtaining desired antibody candidates through experimental approaches can be modest, necessitating iterative cycles of selection and optimization. With ongoing advancements in technology, in silico design of diverse antibody libraries, screening and identification of potential candidates for in vitro validation can be accelerated. To meet this need, we have developed rAbDesFlow, a unified computational workflow for recombinant antibody engineering with open-source programs and tools for ease of implementation. The workflow encompasses five computational modules to perform antigen selection, antibody library generation, antigen and antibody structure modeling, antigen-antibody interaction modeling, structure analysis, and consensus ranking of potential antibody sequences for synthesis and experimental validation. The proposed workflow has been demonstrated through design of rAbs for the ovarian cancer antigen Mucin-16 (CA-125). This approach can serve as a blueprint for designing similar engineered molecules targeting other biomarkers, allowing for a simplified adaptation to different cancer types or disease-specific antigens.

Comparative study of iodine-doped and undoped pyrrole grafting with plasma on poly (glycerol sebacate) scaffolds and its human dental pulp stem cells compatibility.

This study addresses the morphological and chemical characterization of PGS scaffolds after (6, 12, 18, 24, and 30 min) residence in undoped pyrrole plasma (PGS-PPy) and the evaluation of cell viability with human dental pulp stem cells (hDPSCs). The results were compared with a previous study that used iodine-doped pyrrole (PGS-PPy/I). Analyses through SEM and AFM revealed alterations in the topography and quantity of deposited PPy particles. FTIR spectra of PGS-PPy scaffolds confirmed the presence of characteristic absorption peaks of PPy, with higher intensities observed in the nitrile and -C≡C- groups compared to PGS-PPy/I scaffolds, while raman spectra indicated a lower presence of polaron N+ groups. On the other hand, PGS scaffolds modified with PPy exhibited lower cytotoxicity compared to PGS-PPy/I scaffolds, as evidenced by the Live/Dead assay. Furthermore, the PGS-PPy scaffolds at 6 and 12 min, and particularly the PGS-PPy/I scaffold at 6 min, showed the best results in terms of cell viability by the fifth day of culture. The findings of this study suggest that undoped pyrrole plasma modification for short durations could also be a viable option to enhance the interaction with hDPSCs, especially when the treatment times range between 6 min and 12 min.

Prospects for the computational humanization of antibodies and nanobodies.

To be viable therapeutics, antibodies must be tolerated by the human immune system. Rational approaches to reduce the risk of unwanted immunogenicity involve maximizing the 'humanness' of the candidate drug. However, despite the emergence of new discovery technologies, many of which start from entirely human gene fragments, most antibody therapeutics continue to be derived from non-human sources with concomitant humanization to increase their human compatibility. Early experimental humanization strategies that focus on CDR loop grafting onto human frameworks have been critical to the dominance of this discovery route but do not consider the context of each antibody sequence, impacting their success rate. Other challenges include the simultaneous optimization of other drug-like properties alongside humanness and the humanization of fundamentally non-human modalities such as nanobodies. Significant efforts have been made to develop in silico methodologies able to address these issues, most recently incorporating machine learning techniques. Here, we outline these recent advancements in antibody and nanobody humanization, focusing on computational strategies that make use of the increasing volume of sequence and structural data available and the validation of these tools. We highlight that structural distinctions between antibodies and nanobodies make the application of antibody-focused in silico tools to nanobody humanization non-trivial. Furthermore, we discuss the effects of humanizing mutations on other essential drug-like properties such as binding affinity and developability, and methods that aim to tackle this multi-parameter optimization problem.

Deformable Object Manipulation in Caregiving Scenarios: A Review

This paper reviews the robotic manipulation of deformable objects in caregiving scenarios. Deformable objects like clothing, food, and medical supplies are ubiquitous in care tasks, yet pose modeling, control, and sensing challenges. This paper categorises caregiving deformable objects and analyses their distinct properties influencing manipulation. Key sections examine progress in simulation, perception, planning, control, and system designs for deformable object manipulation, along with end-to-end deep learning’s potential. Hybrid analytical data-driven modeling shows promise. While laboratory successes have been achieved, real-world caregiving applications lag behind. Enhancing safety, speed, generalisation, and human compatibility is crucial for adoption. The review synthesises critical technologies, capabilities, and limitations, while also pointing to open challenges in deformable object manipulation for robotic caregiving. It provides a comprehensive reference for researchers tackling this socially valuable domain. In conclusion, multi-disciplinary innovations combining analytical and data-driven methods are needed to advance real-world robot performance and safety in deformable object manipulation for patient care.

Design and testing of a humanized porcine donor for xenotransplantation

Recent human decedent model studies1,2 and compassionate xenograft use3 have explored the promise of porcine organs for human transplantation. To proceed to human studies, a clinically ready porcine donor must be engineered and its xenograft successfully tested in nonhuman primates. Here we describe the design, creation and long-term life-supporting function of kidney grafts from a genetically engineered porcine donor transplanted into a cynomolgus monkey model. The porcine donor was engineered to carry 69 genomic edits, eliminating glycan antigens, overexpressing human transgenes and inactivating porcine endogenous retroviruses. In vitro functional analyses showed that the edited kidney endothelial cells modulated inflammation to an extent that was indistinguishable from that of human endothelial cells, suggesting that these edited cells acquired a high level of human immune compatibility. When transplanted into cynomolgus monkeys, the kidneys with three glycan antigen knockouts alone experienced poor graft survival, whereas those with glycan antigen knockouts and human transgene expression demonstrated significantly longer survival time, suggesting the benefit of human transgene expression in vivo. These results show that preclinical studies of renal xenotransplantation could be successfully conducted in nonhuman primates and bring us closer to clinical trials of genetically engineered porcine renal grafts.

Development of a multi-modal graphene nanoparticles (GNP)- Polydimethylsiloxane (PDMS) flexible sensor for human activity monitoring and health assessment

Recent technological advancements have popularized flexible sensors, which can function as highly sensitive electronic skin, detecting motions and pressure with precision. By utilizing a Polydimethylsiloxane (PDMS) base to encapsulate the functional layer, this paper demonstrates a flexible strain sensor fabricated on Polydimethylsiloxane (PDMS) base encapsulating the functional layer. In this paper, a strain sensor using functional materials Graphene Nanoplatelets (GNP) and PDMS substrate has been proposed and fabricated. The proposed sensor has been analyzed in terms of sensitivity, stability, response time and human compatibility. This sensor showed improved stretchability, flexibility and sensing performance, which can detect both fine and large strains with stability and repeatability. Human compatibility test revealed that it finds application in wearable electronics, biomedical equipment etc.

The enhancement of mechanical properties and uniform degradation of electrodeposited Fe-Zn alloys by multilayered design for biodegradable stent applications.

Pure Fe is a potential biodegradable stent material due to its better biocompatibility and mechanical properties, but its degradation rate needs to be improved. Alloying with Zn to form Fe-Zn alloy is anticipated to meet the degradation rate requirements while retaining the iron's inherent properties. Therefore, Fe-Zn alloys with monolayered and multilayered structures were prepared by electrodeposition. The alloys' composition, microstructure, mechanical properties, in vitro degradation and biocompatibility were assessed. Results showed that the Zn content ranged from 2.1 wt% to 11.6 wt%. After annealing at 450°C, all the alloys consisted of α(Fe) solid solution and Zn-rich B2 ordered coherent phase, except for the alloy with 11.6 wt% Zn content, in which a Fe3Zn10 phase appeared. The layered structure consisted of alternating columnar-grain and nano-grain layers, which compensated for the intrinsic brittleness of electrodeposited metals and improved the galvanic effect of the alloy, thus increasing the strength and plasticity and changing the corrosion from localized to uniform while augmenting the corrosion rate. The yield strength of the multilayered alloy exceeded 350 MPa, its elongation was more than 20%, and its corrosion rate obtained by immersion test in Hank's solution reached 0.367 mm·y-1. Fe-Zn alloys with lower Zn content had good cytocompatibility with the human umbilical vein endothelial cells and good blood compatibility. The above results verified that the multilayered Fe-Zn alloy prepared by electrodeposition presented enhanced mechanical properties, higher degradation rate, uniform degradation mechanism and good biocompatibility. It should be qualified for the application of biodegradable stents. STATEMENT OF SIGNIFICANCE: A potential biodegradable Fe-Zn alloy, which is difficult to be obtained by the metallurgical method, was prepared by electrodeposition to solve the low degradation rate of iron-based biomaterials. A multilayered microstructure design composed of alternating columnar-grain and nano-grain layers was achieved by changing the electrical parameters. The layered design compensated for the intrinsic poor plasticity of electrodeposited metals. It increased the galvanic effect of the alloy, thus augmenting the corrosion rate and changing the corrosion mode of the alloy from localized to uniform corrosion. The yield strength of multilayered alloy exceeded 350 MPa; its elongation was more than 20%. Moreover, the layered alloy had good cytocompatibility and blood compatibility. It indicates that the alloy is qualified for biodegradable stent application.

Human Leukocyte Antigen Alleles Compatibility and Immunophenotypic Profile Associations in Infertile Couples.

The maternal immune system has a major role in the successful embryo implantation and maintenance of the pregnancy. This study aimed to investigate the maternal immunophenotyping profile (percentage of Natural Killer [NK] cellsand the CD4/CD8 [cluster designation] ratio in peripheral blood lymphocytes)and the HLA (Human Leukocyte Antigen)-DQA1 alleles sharing in infertile couples. This cross-sectional study included 78 women who had experienced at least two spontaneous miscarriages and 110 women with a history of recurrent implantation failures after in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and embryo transfer (ET) (IVF-ET failures). The NK cell percentage and the CD4/CD8 ratio were determined by flow cytometry. Genotyping of the HLA-DQA1alleles was carried out for all women and their partners, and couple HLA-DQA1 compatibility was expressed as the percentage of commonHLA-DQA1 alleles (totaling 35 alleles)shared between spouses to the sum of the unique alleles observed. In women with recurrent miscarriages, high values (%) of the NK population with a median (interquartile range [IQR]) of 10.3% (7.7% to 12.5%) and CD4/CD8 ratio (1.7) (1.5 to 2.1) were found. In women with IVF-ET failures, the (%) NK population (10.5%) (8.6% to 12.5%) and CD4/CD8 ratio (1.8) (1.5 to 2.1) were similarly increased (p=0.390, and p=0.490, respectively). The proportion of women with >10% NK cells was 53.8% and 58.2% in women with miscarriages and IVF-ET failures, respectively (p=0.554). The prevalence of HLA-DQA1*5 allele carriage was elevated in women with miscarriages as well as those with IVF-ET failures (52.6% and 61.8%, respectively; p=0.206). The proportion of couples with high (>50%) HLA-DQA1 sharing was 65.4% in the group with miscarriages and 73.6% in the group with IVF-ET failures, respectively (p=0.222). The CD4/CD8 ratio was statistically significantly positively correlated with the (%) NK population in women with IVF-ET failures (rho = 0.297, p=0.002) and with the (%) HLA-DQA1 sharing in the group with miscarriages (rho = 0.266, p=0.019). The couples in which both spouses were carriers of the HLA-DQA1*5 allele had an increased probability of high (>50%) HLA-DQA1 compatibilitycompared with the couples in which neither of the spouses carried the allelein the miscarriage group (OR = 24.3, 95% CI: 3.0 to 198.9, p<0.001), and the IVF-ET failure group (OR = 10.5, 95% CI: 2.2 to 49.8, p<0.001). The peripheral NK (%) population and CD4/CD8 ratio, as well as the prevalence of the HLA-DQA1*5 allele, were elevated in women with recurrent miscarriages and IVF-ET failures. Furthermore, these couples with negative reproductive outcomes had a high percentage of HLA-DQA1 allele similarity. The presence of the HLA-DQA1*5 allele in spouses was strongly associated with overall couple HLA-DQA1 compatibility, implying that it could be used as a surrogate marker for assessing overall immunological compatibility in infertile couples.

Body Shape-Aware Object-Level Outfit Completion for Full-Body Portrait Images

Modeling fashion compatibility between different categories of items and forming personalized outfits have become important topics in recommender systems recently. However, item compatibility and outfit recommendation have been explored in perfect settings in the past, where high-quality images of items from the front view or user profiles are available. In this paper, we propose a new task called Complete The full-body Portrait (CTP) for real-world fashion images (e.g., street photos and selfies), which is able to recommend the most compatible item for a masked scene where the outfit is incomplete. Visual compatibility and personalization are the key points for accurate scene-based recommendations. In our approach, the former is accomplished by calculating the visual distance of the query scene and target item in latent space, while the latter is achieved by taking the body-shape information of the human subject into consideration. To obtain side information to train our model, ResNet-50, YOLOv3 and SMPLify-X models are adopted to extract visual features, detect item objects, and reconstruct a 3D body mesh, respectively. Our approach first predicts the missing item category from the masked scene, and then finds the most compatible items from the predicted category through computing visual distances at image level, region level and object level, together with measuring human body-shape compatibility. We conduct extensive experiments on two real-world datasets, Street2Shop and STL-Fashion. Both quantitative and qualitative results show that our model outperforms all baseline models.

Donor selection for adoptive cell therapy with CD45RA− memory T cells for patients with coronavirus disease 2019, and dexamethasone and interleukin-15 effects on the phenotype, proliferation and interferon gamma release

Background aimsWe have previously demonstrated the safety and feasibility of adoptive cell therapy with CD45RA− memory T cells containing severe acute respiratory syndrome coronavirus 2–specific T cells for patients with coronavirus disease 2019 from an unvaccinated donor who was chosen based on human leukocyte antigen compatibility and cellular response. In this study, we examined the durability of cellular and humoral immunity within CD45RA− memory T cells and the effect of dexamethasone, the current standard of care treatment, and interleukin-15, a cytokine critically involved in T-cell maintenance and survival. MethodsWe performed a longitudinal analysis from previously severe acute respiratory syndrome coronavirus 2–infected and infection-naïve individuals covering 21 months from infection and 10 months after full vaccination with the BNT162b2 Pfizer/BioNTech vaccine. ResultsWe observed that cellular responses are maintained over time. Humoral responses increased after vaccination but were gradually lost. In addition, dexamethasone did not alter cell functionality or proliferation of CD45RA- T cells, and interleukin-15 increased the memory T-cell activation state, regulatory T cell expression, and interferon gamma release. ConclusionsOur results suggest that the best donors for adoptive cell therapy would be recovered individuals and 2 months after vaccination, although further studies with larger cohorts would be needed to confirm this finding. Dexamethasone did not affect the characteristics of the memory T cells at a concentration used in the clinical practice and IL-15 showed a positive effect on SARS-CoV-2-specific CD45RA- T cells.

Structural basis of human SNAPc recognizing proximal sequence element of snRNA promoter

In eukaryotes, small nuclear RNAs (snRNAs) function in many fundamental cellular events such as precursor messenger RNA splicing, gene expression regulation, and ribosomal RNA processing. The snRNA activating protein complex (SNAPc) exclusively recognizes the proximal sequence element (PSE) at snRNA promoters and recruits RNA polymerase II or III to initiate transcription. In view that homozygous gene-knockout of SNAPc core subunits causes mouse embryonic lethality, functions of SNAPc are almost housekeeping. But so far, the structural insight into how SNAPc assembles and regulates snRNA transcription initiation remains unclear. Here we present the cryo-electron microscopy structure of the essential part of human SNAPc in complex with human U6-1 PSE at an overall resolution of 3.49 Å. This structure reveals the three-dimensional features of three conserved subunits (N-terminal domain of SNAP190, SNAP50, and SNAP43) and explains how they are assembled into a stable mini-SNAPc in PSE-binding state with a “wrap-around” mode. We identify three important motifs of SNAP50 that are involved in both major groove and minor groove recognition of PSE, in coordination with the Myb domain of SNAP190. Our findings further elaborate human PSE sequence conservation and compatibility for SNAPc recognition, providing a clear framework of snRNA transcription initiation, especially the U6 system.

Mechanical properties and in vitro human gingival fibroblasts compatibility of plasma-sprayed zirconia-coated titanium alloy abutment

A zirconia coating on the titanium alloy was prepared using plasma spray technique. The aim was to investigate the mechanical properties of zirconia coatings and the biocompatibility with human gingival fibroblasts (HGFs), which determines early soft tissue sealing. The following groups were examined: (1) medical grade 5 titanium alloy (T), (2) sintered zirconia (Z), and (3) plasma-sprayed zirconia-coated titanium (Z-T). The results indicated that the plasma-sprayed zirconia-coating had good mechanical properties, and it enhanced the bioactivity of HGFs.

Research progress of fibre-based organic electrochemical transistors

&lt;p&gt;Organic electrochemical transistors are flexible in design with characteristics such as miniaturisation, biocompatibility and amplification and are one of the rapidly developing research topics in recent years. As an excellent flexible material, fibre has unparalleled advantages in weaving and compatibility with the human body. Combining fibres with organic electrochemical transistors is a promising research direction that has the high sensitivity of organic electrochemical transistor testing and the human body compatibility and flexibility of wearable electronic products. This paper introduces the relevant operating principles, working modes and commonly used channel materials of organic electrochemical transistors. Based on the basic device structure of organic electrochemical transistors, the development and changes of organic electrochemical transistors in recent years are discussed, and the research results of fibre-based electrochemical transistors by researchers focusing on the application of fibre-based organic electrochemical transistors in chemical sensing, bio-sensing and other application explorations are summarized. Finally, this paper visioned the future development trend of fibre-based organic electrochemical transistors.&lt;/p&gt;

When ill is not ill enough—timeframe until expected death restrictions in Australian Voluntary Assisted Dying laws and human rights compatibility

ABSTRACT In the past, aiding another person in ending their life or taking the life of another, even upon their request and with their consent, gave rise to criminal liability in all Australian jurisdictions. Consequently, neither doctors nor relatives could lawfully assist someone in dying. Since 2017, five Australian jurisdictions have introduced Voluntary Assisted Dying (‘VAD’) Acts allowing eligible individuals to end their lives with assistance. A person seeking access to VAD in Victoria, Western Australia, Tasmania, South Australia and Queensland must meet specific eligibility requirements including be diagnosed with a disease, illness or medical condition which is advanced, progressive and is expected to cause death within a specific timeframe ranging from six to 12 months depending on the jurisdiction. Using Queensland as an exemplar, this article analyses whether the VAD timeframe until death restriction limits individual human rights in Australian jurisdictions with human rights legislation and contemplates whether potential limitations are reasonable and justified. The article concludes that due to its severe impact on individuals wishing to end their life with assistance, the restriction is raising severe concerns about its human rights compatibility.

COVID-19, International Human Rights Law and the State-Corporate Complex

Abstract Data-driven technologies (such as mobile phone-based tracing apps) have been at the forefront of public health responses to the COVID-19 pandemic. However, we have also seen high-level expressions of concern about how state actions ostensibly in pursuit of public health goals have in fact greatly accelerated existing human rights concerns about newer technologies, especially increased state and corporate surveillance. This article explores issues at the nexus of COVID-19 public health responses, civil-political rights under international human rights law, and the responsible governance of data-driven technologies. In particular, it offers a framework to evaluate the human rights compatibility of tech-assisted COVID-related state measures. The articles also explore analogies between COVID-related measures and post-2001 counter-terrorism actions taken by states in the name of public security. It cautions against exceptional measures becoming hardwired in ways that may unreasonably impact on pre-COVID freedoms. The article argues that the blurring of state and corporate surveillance and data-gathering and the often symbiotic relationship between tech firms and governments (the ‘state-corporate complex’) complicate efforts to assert clear frames of responsibility.

Flexible enzymatic biofuel cell based on 1, 4-naphthoquinone/MWCNT-Modified bio-anode and polyvinyl alcohol hydrogel electrolyte

To meet the emerging power demand of microelectronic and electronic skin based sensing platform, enzymatic fuel cells have received increasing attention due to their good human's compatibility, easy integration and cost effectiveness. Herein, we use multi-walled carbon nanotube/naphthoquinone to modify the lactate oxidase bio-anode to facilitate the electron transfer between electrocatalytic active site and electrode support. Polyvinyl alcohol hydrogel serves as the separator and lactate container. The bio-anode, Pt/C cathode and hydrogel are assembled in layer-by-layer structure, which can successfully utilize pre-stored and external lactate from human's sweat to generate the electricity. It delivers a power-density of 62.2 ± 2.4 μW cm−2 under bending/torsion conditions. Given that the broad substrate scope in sweat and easily assembled structure, it provides a plausible solution to power the miniaturized sensors and generic circuits.