Functions Of Human Body Research Articles

Plasma processed Zn fortification: the next generation sustainable technology for the improvement of agronomic traits of paddy

Zinc performs different metabolic functions in human body and its deficiency is a major concern mainly in the third world countries. Fortification of Zn in grain crops may have the possibility to meet the challenge. The present study was designed utilizing plasma technology for paddy crop in two ways: (i) seed treatment with gliding arc argon-oxygen-ZnSO4-H2O discharge plasma jet in combination with nebulizing system for the fortification of Zn in the seeds, and (ii) plasma treated water (PTW) was sprayed to the plants cultivated from treated seeds and thereby investigated the growth and development of paddy plants defence system, nutritional composition and yield of paddy. Gliding arc argon-oxygen-ZnSO4-H2O discharge plasma jet was employed to treat paddy seeds with nebulizing system for the duration of 2-, 4-, 6-, and 8 min and the 4 min treatment condition provided the maximum 98% germination among the conditions considered. Seedlings germinated from the 4 min treated seeds were resettled in the research area and PTW were applied two, four, and six times at the time of their growing period. The results reveal that the growth parameters, and the plants defence mechanisms were enhanced. Further, Zn concentration in the grains and the yield of paddy were increased by 59.07%, 24.06% and 38.94%, 22.27% respectively, compared to control and the plants grown from the treated seeds where no PTW foliar spray was applied. This investigation exposed that the fortification of Zn in seed by plasma processing and PTW spray to the plants which in turn improved seed germination, enzymatic activities, Zn accumulation in rice, and yield. It is fascinating that the paddy harvesting time was reduced more than two weeks compared to control.

Assessment of Prognostic Factors, Clinical Features Including the Microbiome, and Treatment Outcomes in Patients with Cancer of Unknown Primary Site

Introductions: cancer of unknown primary site (CUP) is a heterogeneous group of cancers in which metastases are found, and the primary tumor is not detected with available diagnostic methods. CUP is a disease that has not been fully researched, and its biology is unclear. The clinical characteristics of CUP are variable, but the prognosis of patients is usually unfavorable, and the possibilities of radical treatment are limited. The microbiome is the genes and gene products of microorganisms residing in a human body. In recent years, thanks to the use of next-generation sequencing, it is possible to assess the impact of the microbiome on human body functions. Head and neck cancers, due to the rich microbiome of this area, are influenced by it, and dysbiosis may be a risk factor for the development of cancer. Objective of this work: the aim of this study was to evaluate prognostic factors, clinical features including the microbiome, and treatment outcomes in patients with cancer of unknown primary site. Results: in the study group, increased numbers of bacteria of the phyla Bacteroides, Fusobacteria, Bacillota, Actinomycetota, Actinobacteria, and Candidatus were detected, while Firmicutes and Proteobacteria were detected in smaller numbers. Independent predictors of CUP occurrence were the following: leukocyte count of at most 6.49 × 103/mm, bacteria from the Proteobacteria phylum in the microbiome below 11.6%, Firmicutes below 22.1%, and Actinobacteria at least 11.0%. Increased numbers of Porphyromonas and Fusobacterium bacteria were associated with the risk of radiotherapy complications and shortened survival rate. Conclusions: clinical diagnosis and treatment of patients with CUP is complicated and difficult due to the lack of consensus on this issue. Treatment and prognosis of patients with CUP is unsatisfactory. The clinical value of the influence of the microbiome on the development, course, and treatment of cancer is becoming increasingly important. The microbiome may become a marker of response to anticancer treatment and the risk of its complications. Immunity modulation with the microbiome provides opportunities for further research on improving the effectiveness of oncological treatment. Fusobacterium and Porphyromonas seem to be the bacteria most important for the development of cancer, also worsening the prognosis of patients by increasing the risk of complications of radiotherapy and shortening the survival rate of patients. Streptococcus and Lactobacillus seem to be bacteria that reduce the risk of cancer, reduce the risk of complications, and improve the prognosis of patients. Total protein deficiency and elevated inflammatory markers are also important predictors of cancer risk.

Physics and medicine from antiquity to the medieval period

Abstract Medical physics is commonly held to have begun at the end of the nineteenth century following discoveries about radiation and radioactivity that were to transform medical diagnosis and the treatment of cancer. A wider perspective dates the origin of the relationship between physics and medicine to the scientific revolution of the sixteenth century when physics began to be applied to understanding the workings of the body and to the development of medical technology. This paper argues that the relationship is in fact much older, dating back several millennia. At the heart of ancient Greek φυσική (natural philosophy) was an attempt to understand the nature and composition of the Universe and a belief which remains valid today that the human body functions on the same principles as the Universe as a whole. This approach led to a corpus of medical teachings rooted in physics as it was then understood that remained dominant in Europe and the Islamic world until the sixteenth century. Furthermore, ancient and medieval sources provide evidence of physical principles being used in medical diagnosis, in treatment and as a means of understanding the functioning of the body in a range of ancient cultures including Greece, Egypt and Persia. This mirrors the different ways in which physics continues to contribute to medical science and clinical practice today. Examples described in this paper include the first technique to map body temperature (arguably also the first medical imaging technique), the use of fire and heat in surgery and other forms of treatment, and medieval attempts to understand vision and the physics of the eye.

High-altitude cerebral oxygen saturation detection using wireless wearable cerebral oximeter.

Hypobaric hypoxic conditions encountered at high altitudes can significantly impact the physiological functions of human body. Therefore, accurate and real-time monitoring of physiological characteristics is crucial for the prevention of brain injuries in individuals with acute and chronic high-altitude exposure. In this study, a wireless wearable cerebral oximeter (WORTH band) was used for the continuous, real-time monitoring of physiological parameters, including regional cerebral oxygen saturation (rSO2) and heart rate (HR), among subjects with high-altitude exposure. During the high-altitude (from 46 m to 4300 m) expedition task, there was a significant decrease in rSO2 accompanied by a corresponding increase in heart rate as the altitude increased. Additionally, during the long-term (52 days) high-altitude (from 356 m to 4658 m) cycling task, the altitudes were significantly correlated with the rSO2 and SpO2 in the elderly subjects. The current findings indicate that the WORTH band oximeter can serve as a promising instrument for measuring rSO2 at high altitudes. We hope that the insights derived from this study could contribute to the management of cerebral oxygenation for individuals with high-altitude exposure and further expand the existing understanding of brain functional detection at high altitudes.

Chromosome Gene, Hsl7 Gene in Vitro Studies

Chromosome gene can expression many functions in human body. Eukaryotic cells are presence in yeast and human, in order to more understanding human gene, we use yeast as a model to research. The ORF (open reading frame) position of the Hsl7 gene is in yeast Saccharomyces YBR133C between 501804bp~504287bp of Chromosome II, with a total length of 2483bp, and the protein size is 95 kDa. Hsl7 gene is a methyltransferase. Protein-arginine N-methyltransferase involved in protein catabolism and regulation of the G2/M transition of mitosis; localizes to the septin collar of the bud neck, and the outer plaque of the spindle pole body in the G1 phase of the cell cycle (Saccharomyces Genome Database), Hsl7 (histone synthetic lethal 7) represent protein methylation, protein methylation is a type of post-translational modification that involves the addition of methyl groups to proteins, Protein methylation, including histone methylation and non-histone protein methylation, is one of the most important forms of posttranscriptional and epigenetic modifications.

Investigating the User Experience Design of Home Ventilators Through the Lens of Kansei Engineering

Abstract Obstructive sleep apnea hypopnea syndrome (OSAHS) is a disease characterized by respiratory disorders, and its prevalence rate is on the rise worldwide. Home ventilator can replace or enhance the respiratory function of human body and alleviate the symptoms of OSAHS, and has become an important treatment, rehabilitation, and nursing equipment for patients. However, the existing products lack humanistic care in emotional level, which leads to negative emotions in the process of human–computer interaction. This study seeks to enhance the user experience of home ventilators by applying the principles of Kansei Engineering (KE). Through a principal component analysis of nine pairs of Kansei image adjectives associated with home ventilators, we concluded that users prefer home ventilators that are “friendly,” “technological,” “pleasant ,” “orderly ,” and “easy to understand.” These findings were utilized in the Kansei design of home ventilators. The effectiveness of these conclusions in improving the user experience of ventilators was subsequently validated using the PrEmo method.

Preuniversity Students' Perceptions and Attitudes About an Anatomy and Physiology Outreach Program: Survey Study and Inductive Thematic Analysis.

Science and health outreach activities are aimed at motivating and sparking interest among prospective students to pursue careers in these fields; however, research studies supporting this hypothesis are limited. The aim of our study was to organize an integrated Anatomy and Physiology outreach to examine preuniversity students' learning experiences (learning tools, activities, and facilitators) and motivation to pursue a career in health care and to gather evidence on their attitudes and perceptions of such activities. A 2-day course on cardiorespiratory and gastrointestinal anatomical systems was presented at the Lee Kong Chian School of Medicine in Singapore using its key pedagogies, that is, multimodal practical and team-based learning. Ninety preuniversity students from 21 preuniversity institutions in Singapore participated in this 2-day course, and their experiences were evaluated using a 4-point Likert scale and open-ended survey questions. Free-text comments were analyzed using inductive thematic analysis. The survey using the 4-point Likert scale was completed by 81 (92%) of the 88 participants. Most students felt that the course materials were adequate (mean 3.57, SD 0.57) and met the learning objectives (mean 3.73, SD 0.52). The students felt that the instructors were clear (mean 3.73, SD 0.52) and effective (mean 3.70, SD 0.53). They liked the organization of the outreach session (mean 3.64, SD 0.48) and were highly motivated to study medicine or allied/biomedical sciences (mean 3.69, SD 0.54). Practical and team-based learning were regarded as exceedingly satisfactory (mean 3.63, SD 0.53 and mean 3.58, SD 0.54, respectively). All the respondents said that they would recommend this course to peers. Thematic analysis revealed that the participants gained a new perspective of the human body structure and function, they liked the unique learning settings, they were motivated to pursue a career in health care, they were satisfied with the sessions, and interactions with the facilitators increased their understanding of the human anatomy and physiology. Structured health outreach activities provide students with unique opportunities to experience a preclinical learning environment in a medical school, deepen their understanding of human body structure and function, and increase their motivation and interest in science. Further, outreach programs may lay the foundations for potential students aiming to pursue health profession education.

Research activity of the kyiv anatomical school: from origins to the present day

The history of the Department of Human Anatomy at the O. O. Bogomolets National Medical University demonstrates the significant contribution of prominent individuals to the development of Ukrainian anatomy, who devoted their lives to scientific and educational work, nurtured successors, and passed on their knowledge and experience. Invaluable contributions to the advancement of anatomical science were made by scholars who facilitated the refinement of research methods, the development of innovative technologies, and practical applications of acquired knowledge. The anatomical school of Kyiv distinguished itself by establishing the key principle of the interdependence of form and function, leading to the integration of theory and practice into a unified system. This was a crucial step towards further progress in the field of human body structure and function science. Such an approach allowed the merging of theoretical knowledge with practical reality, enhancing the more effective utilization of acquired data in medical practice. Anatomical science is constantly evolving and changing. Scientists not only enhance objective research and diagnostic methods but also make significant contributions to conducting clinical studies and developing innovative treatment techniques. An essential aspect of anatomy development is its connection to the practice of Ukrainian healthcare. Advances in anatomy contribute to improving the quality of medical services and ensure more effective patient treatment. Anatomy experts continue to work on improving education methods and medical personnel training to secure the future health and well-being of the nation. The Ukrainian anatomical school continues to evolve and maintain high standards in the field of medical science, providing a comprehensive approach to studying the structure and functions of the human body. Its achievements and contributions to the advancement of global anatomical science are of utmost importance to the medical community and society as a whole.

The analysis of the essential metal contents in khat (Catha edulis Forsk) from Meru County, Kenya

BackgroundChemical composition of plants has attracted considerable interest in recent years with considerable research tailored into determining metal contents in food substances. Particularly, essential elements which are necessary for normal human body functioning and are strongly recommended in optimal proportions for better health. This study determined the levels of essential elements; calcium (Ca), magnesium (Mg), sodium (Na), potassium (K) and manganese (Mn) in eleven khat (Catha edulis Forsk) samples collected from Meru County. Methodology1.0 g of dry ground khat samples were wet-digested in a solvent mixture of 10 mL of HCl, HNO3 and 20 vol of H2O2 for 3 h at variable temperature of 50–90 °C and later reconstituted in 25 mL 0.05 molL−1 HCl before analysis. The samples were analyzed using inductively coupled plasma atomic emission spectrometry (ICP AES) after acid digestion whereas Hierarchical cluster analysis (HCA) and principal component analysis (PCA) were used to investigate elemental characteristics and determine the sources of essential metals in khat. In addition, Pearson correlation coefficient (PCC) was used to evaluate the relationships between the essential metals. ResultsSpinach leaves certified reference material (CRM 1750a) was analyzed and the percentage recoveries (%) of 98.4 and 101.7 determined showing that the digestion method employed in this work had adequate accuracy. The validated method was employed for the analysis of all the khat samples. The analyzed metal concentration ranges (mg100g−1) in fresh-weight basis per day of five metals were 160.9–267.3, 44.6–81.7, 2.0–3.0, 237.6–393.6, and 220.3 -349.0 for Ca, Mg, Mn, K, and Na, respectively. Both HCA and PCA results showed that these elements could be linked to natural soils and anthropogenic sources. PCC reported significant correlations between Na and K indicating they may have been derived from the same source. The daily metal intakes were below the World Health Organization (WHO) permissible limits implying no associated health risks for khat users. ConclusionBased on the findings of this study, the consumption of khat from regions of Meru County may be a potential source for essential elements required for human biological processes.

Biological analysis method based on the relationship between athlete’s PSI rhythm and sports injury

Biorhythm is the law of cyclical changes in individual life activity characteristics as time changes. Biorhythm is an internal law of a biological organism, which comes from the changes that a biological organism is affected by changes in the external environment during natural activities, so its change law is similar to the cycle of the natural environment. Biological rhythms play an important role in mediating human body functions, vital activities, body temperature and other physiological reactions. The balance and stability of biological rhythms can ensure the healthy life activities of the human body. Disrupting the balance of biological rhythms may cause various kinds of human body. In this paper, the research of biological analysis method based on the relationship between athlete’s PSI (Physical, Sensitive, Intellectual) rhythm and sports injury is mainly introduced, and ideas and directions are intended to be provided for analyzing the relationship between athlete’s physical strength, emotion, intelligence and sports injury. This paper proposes a research strategy based on the biological analysis method of athlete’s PSI rhythm and sports injury, including measurement of biological rhythm, literature retrieval method, expert interview method, questionnaire survey method, clustering algorithm-based biological analysis method. A research experiment on the biological analysis method of the relationship between PSI rhythm and sports injury is conducted. The experimental results of this paper indicated that most sports injuries of athletes occur in the critical period of PSI rhythm, with an average incidence of 70.14%. Athletes’ training and competition can be avoided in this critical period as much as possible.

Highly stable Tb(III) metal-organic framework derived from a new benzothiadiazole functionalized ligand for fluorescence recognition of ascorbic acid

Because ascorbic acid (AA) is one of the basic elements to maintain the normal physiological functions of human body, it is urgent to develop a material that can achieve efficient, rapid and in-situ detection for AA. A new fluorescence organic compound 4′,4′′′-(benzo[c][1,2,5]thiadiazole-4,7-diyl)bis([1,1′-biphenyl]-4-carboxylic acid) (H2BTBC) based on benzothiadiazole group has been synthesized, which can detect Fe3+ ions by fluorescence turn-off effect with a detection limit of 0.015 μM, as well as recognize linear amines by fluorescence turn-on effect. Moreover, a highly stable Tb(III) metal–organic framework has been solvothermally prepared with H2BTBC, namely {[(CH3)2NH2]2[Tb2(BTBC)4]∙solvents}n (JXUST-39), which can selectively detect AA among biological fluids by fluorescence enhancement effect with a detection limit of 0.077 μM. In addition, the mechanism for JXUST-39 detecting AA is possibly the cooperative effect of absorbance-caused enhancement and charge transfer between JXUST-39 and AA. Moreover, LED lamp beads, fluorescent films and fluorescent detection test paper based on JXUST-39 were prepared to achieve portable detection via fluorescence enhancement effect.

Jejunal transcriptomic profiling of carnosine synthesis precursor-related genes and pathways in slow-growing Korat chicken

Carnosine is a physiologically important molecule in normal human body functions. Chicken meat is an excellent source of carnosine; especially slow-growing Korat chicken (KR) females have a high carnosine content in their meat. The carnosine content of chicken meat can be increased by dietary supplementation of β-alanine (βA) and L-histidine (L-His). Our objective was to reveal the pathways and genes through jejunal transcriptomic profiling related to βA and L-His absorption and transportation. We collected whole jejunum samples from 5 control and 5 experimental KR chicken, fed with 1% βA and 0.5% L-His supplementation. A total of 407 differentially expressed genes (P < 0.05, log2 fold change ≥2) were identified, 272 of which were down-regulated and 135 up-regulated in the group with dietary supplementation compared to the control group. Based on the integrated analysis of the protein-protein interaction network and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway maps, 87 gene ontology terms were identified and 6 KEGG pathways were significantly (P < 0.05) enriched in the jejunum. The analyses revealed 6 key genes, KCND3, OPRM1, CCK, GCG, TRH, and GABBR2, that are related to neuroactive ligand-receptor interaction and the calcium signaling pathway. These findings give insight regarding the molecular mechanism related to carnosine precursor absorption and transportation in the jejunum and help to identify useful molecular markers for improving the carnosine content in slow-growing KR chicken meat.

Differential SNARE chaperoning by Munc13-1 and Munc18-1 dictates fusion pore fate at the release site.

The regulated release of chemical messengers is crucial for cell-to-cell communication; abnormalities in which impact coordinated human body function. During vesicular secretion, multiple SNARE complexes assemble at the release site, leading to fusion pore opening. How membrane fusion regulators act on heterogeneous SNARE populations to assemble fusion pores in a timely and synchronized manner, is unknown. Here, we demonstrate the role of SNARE chaperones Munc13-1 and Munc18-1 in rescuing individual nascent fusion pores from their diacylglycerol lipid-mediated inhibitory states. At the onset of membrane fusion, Munc13-1 clusters multiple SNARE complexes at the release site and synchronizes release events, while Munc18-1 stoichiometrically interacts with trans-SNARE complexes to enhance N- to C-terminal zippering. When both Munc proteins are present simultaneously, they differentially access dynamic trans-SNARE complexes to regulate pore properties. Overall, Munc proteins' direct action on fusion pore assembly indicates their role in controlling quantal size during vesicular secretion.

Pathogenesis of ferroptosis on chronic kidney disease and traditional Chinese medicine intervention: a review

Chronic kidney disease(CKD) is an insidious disease that has become a significant global public health issue due to its high incidence rate, low awareness, low diagnostic rate, poor prognosis, and high medical costs. Recent studies have shown that CKD development is associated with varying degrees of ferroptosis features. Traditional Chinese medicine(TCM) can regulate iron metabolism, lipid peroxidation, antioxidant systems to inhibit ferroptosis and delay the progression of CKD. Consequently, the intervention mechanism of ferroptosis has become one of the focuses of CKD research. TCM has thousands of years of traditional experience and wisdom. It focuses on the overall regulation of human body functions and can stimulate the body's disease resistance and recovery capabilities, which has certain advantages in treating CKD. However, there is currently a lack of comprehensive articles on the application of TCM in intervening ferroptosis to treat CKD and the pathogenesis of ferroptosis in CKD. Therefore, this article summarizes the latest research progress both domestically and internationally, briefly introduces the main mechanisms of ferroptosis, and systematically reviews the relationship between ferroptosis and CKD. The article integrates TCM theories related to ferroptosis in CKD, including &quot;deficiency&quot; &quot;stasis&quot; &quot;phlegm turbidity&quot; and &quot;toxins&quot; and summarizes the research status of active ingredients and herbal formulas in intervening ferroptosis to treat CKD. By considering ferroptosis from a new perspective, this article aims to provide new targets and directions for the application of TCM in treating CKD.

Analisis Beban Kerja Mental saat Mengoperasikan Smartphone dengan Memperhatikan Postur Tubuh: Pendekatan Menggunakan Metode NASA-TLX

Work physiology is the study of understanding how the human body adapts and functions in the work environment. Occupational physiology involves the study of the body's physiological responses to work-related demands and stress. This understanding is necessary to protect workers' health and welfare, as well as increase work productivity and efficiency. This research aims to analyze physiological workload by focusing on measuring mental workload, using the NASA-TLX method. Research activities were carried out on 12 students from the Department of Industrial Engineering, Universitas Mataram. Students were divided into 3 groups according to their assigned work posture, namely standing (Group A), sitting (Group B), and lying down (Group C). The results showed that the order of subjects with the highest mental workload was subject 1A (78.97%) with a standing posture, 2B (77.33%) with a sitting posture, and 4A (73.57%) with a standing posture. Based on these results, the final conclusion that can be drawn is that the mental workload in standing and sitting postures is what is most felt by the subject when operating the smartphone. These results conclude that different postures when operating a smartphone can affect the user's mental workload Keywords: Mental, NASA-TLX, Posture, Smartphone, Workload.

Nanotechnology - an innovative approach to cope with the distinctive challenges linked with Autism Spectrum Disorder

The brain stands out as the most intricate organ in the human body, governing cognitive, behavioral, and emotional functions. It is susceptible to various diseases, ranging from injuries to cancers and neurodegenerative conditions, making brain disorders a significant cause of disability and mortality. Overcoming challenges such as delivery, specificity, and toxicity has been a persistent issue in developing drugs that enhance brain structure and function, especially those that can traverse the complex barriers surrounding the brain. Nanotechnology represents a groundbreaking approach to address the unique challenges associated with Autism Spectrum Disorder (ASD). One significant challenge in ASD is early and accurate diagnosis. Nanotechnology can contribute to the development of highly sensitive and specific diagnostic tools, enabling the identification of biomarkers associated with ASD at an earlier stage. These nanoscale devices may facilitate a more precise understanding of the underlying biological mechanisms, leading to improved diagnostic capabilities. In terms of intervention, nanotechnology can enhance drug delivery systems, allowing for the more targeted and efficient administration of medications. This targeted drug delivery can potentially mitigate side effects while maximizing the therapeutic impact, addressing some of the challenges in managing the diverse symptoms of ASD. Despite the promising potential of nanotechnology in addressing ASD challenges, it is essential to approach these innovations with ethical considerations, ensuring that the benefits are accessible and equitable for individuals with ASD. Ongoing research and collaboration between experts in nanotechnology and autism can lead to transformative advancements in understanding, diagnosing, and managing ASD. This review delves into the application of nanotechnology in diagnosing and treating ASD and shedding light on the promising role of nanoparticles.

Research progress on the microbiota in bladder cancer tumors.

The microbiota, also referred to as the microbial community, is a crucial component of the human microenvironment. It is located predominantly in various organs, including the intestines, skin, oral cavity, respiratory tract, and reproductive tract. The microbiota maintains a symbiotic relationship with the human body, influencing physiological and pathological functions to a significant degree. There is increasing evidence linking the microbial flora to human cancers. In contrast to the traditional belief that the urethra and urine of normal individuals are sterile, recent advancements in high-throughput sequencing technology and bacterial cultivation methods have led to the discovery of specific microbial communities in the urethras of healthy individuals. Given the prevalence of bladder cancer (BCa) as a common malignancy of the urinary system, researchers have shifted their focus to exploring the connection between disease development and the unique microbial community within tumors. This shift has led to a deeper investigation into the role of microbiota in the onset, progression, metastasis, prognosis, and potential for early detection of BCa. This article reviews the existing research on the microbiota within BCa tumors and summarizes the findings regarding the roles of different microbes in various aspects of this disease.

Recent trends of diabetes and phase angle (PhA) by bioelectrical impedance analysis (BIA)

Recent topics regarding diabetes and Phase Angle (PhA) by the Bioelectrical Impedance Analysis (BIA) method were introduced. Decreased PhA value means impaired cell function of human body. PhA was significantly lower in Type 2 diabetes (T2D). T2D cases of 30-83 years showed average data of PhA 6.84/5.99, and HbA1c 7.35/5.95% as male/female. When male cases were analyzed for lowest quartile with the data of PhA ≤4.9, they showed odds ratio (OR) 2.02 with significantly higher prevalence of diabetes. Regarding diabetic polyneuropathy (DPN), PhA value showed control 6.18, T2D without DPN 6.07 and T2D with DPN 5.71 with significant difference.

Stable and reproducible MIP-ECL sensors for ultra-sensitive and accurate quantitative detection of Estrone.

Estrone (E1), as an endogenous estrogen, has a variety of physiological functions in human body and is of great significance to human health. On the other hand, it is a widely distributed and highly disturbing environmental endocrine disruptor in water. Therefore, there is an urgent need to develop a sensitive, rapid, and inexpensive method for the on-site determination of E1, which is not only for clinical diagnosis and treatment, but also for the investigation and monitoring of endogenous estrogen pollution in environmental water. In this study, Ru(bpy)3 2+/MWCNTs/Nafion/gold electrodes were prepared by surface electrostatic adsorption and ion exchange. A molecularly imprinted membrane (MIP) with the capability to recognize E1 molecules was prepared by sol-gel method, and the electrodes were modified with MIP to form an electrochemical luminescence sensor (MIP-ECL). This method simultaneously possesses ECL's advantage of high sensitivity and MIP's advantage of high selectivity. Moreover, the addition of carboxylated multi-walled carbon nanotubes (MWCNT-COOH) improved the functionalization of the gold electrode surface and increased the binding sites of MIP. Meanwhile, the good conductivity of MWCNTs promoted electron transfer and further improved the sensitivity of the sensor. The sensor showed a wide linear interval in which the E1 concentrations can range from 0.1μg/L to 200μg/L, along with a high linear correlation coefficient (R 2 = 0.999). The linear regression equation of the sensor was Y = 243.64x-79.989, and the detection limit (LOD) was 0.0047μg/L. To validate our sensor, actual samples were also measured by the reference method (LC-MS/MS), and it was found that the relative deviation of quantitative results of the two different methods was less than 4.1%. This indicates that the quantitative results obtained by this sensor are accurate and can be used for rapid in situ determination of E1 in clinical samples and environmental water.

A study of splenic notches in human cadavers and its clinical implications

: Spleen is the largest and secondary lymphoid organ with high vascularity which has important role in immunological and haematological functions of human body. Spleen developes from mesoderm and in the initial stages of development, different lobules are formed, which fuses later. The lobulated structure of spleen disappears, but is indicated by the presence of notches in superior border in adults. The aim of study was to find morphological variations of spleen with respect to its number of notches in borders and anomalous fissure on its surfaces.: The study was done on 40 formalin fixed cadaveric spleens from the department of Anatomy, Sri Siddhartha Medical College, Tumkur, and Karnataka. Out of 40 spleens studied, the various shapes such as wedge shaped, tetrahedral, oval and triangular were found. The number of spleen showing notches in superior border was 28(70%) and in the inferior border it was 3(7.5%). Absence of Splenic notches was observed in 5(12.5%) and 2(5%) spleens had notches in both borders. The anomalous fissure was found in 2(5%) spleen on its diaphragmatic surface.: The knowledge of variations in morphology of spleen are essential for physician, surgeon, radiologists and forensic surgeon to differentiate from the splenic pathology and splenic injury. Morphological variations of spleen and its clinical importance need to be discussed at dissection tables during routine anatomy dissection hours.