HRD practices in private and cooperative sector milk processing organizations in Pune region were investigated through 175 management and 1195 employee respondents of selected milk processing organizations from both the sector by adopting proportionate convenience sampling technique; which indicates that overall, fair & poor level of HRD practices found in private and cooperative milk processing organizations in Pune region; an enlightened organization would initiate immediate drastic HRD interventions to prevent the situation from deteriorating further by designing/re-designing effective HRD policy and developing good HRD practices. Management of both the sector milk processing organizations needs to design the existing HRD policy in line with standard practices so as to develop good HRD culture. Again, in between the two sectors, private and cooperative sector milk processing organizations in Pune region, cooperative sector was noticed to be drag behind by most of the poor HRD practices and indicating the acute need for addressing the issues. It strongly indicates the need for addressing the challenges of HRD practices being followed by implementing all HRD practices as per standard way to get the best HRD practices result in both the sectors milk processing organizations so as to sustain in global competition.