House Mouse Mus Musculus Domesticus Research Articles

The adaptive immune response to Trichuris in wild versus laboratory mice: An established model system in context.

Laboratory model organisms have provided a window into how the immune system functions. An increasing body of evidence, however, suggests that the immune responses of naive laboratory animals may differ substantially to those of their wild counterparts. Past exposure, environmental challenges and physiological condition may all impact on immune responsiveness. Chronic infections of soil-transmitted helminths, which we define as establishment of adult, fecund worms, impose significant health burdens on humans, livestock and wildlife, with limited treatment success. In laboratory mice, Th1 versus Th2 immune polarisation is the major determinant of helminth infection outcome. Here we compared antigen-specific immune responses to the soil-transmitted whipworm Trichuris muris between controlled laboratory and wild free-ranging populations of house mice (Mus musculus domesticus). Wild mice harbouring chronic, low-level infections produced lower levels of cytokines in response to Trichuris antigen than laboratory-housed C57BL/6 mice. Wild mouse effector/memory CD4+ T cell phenotype reflected the antigen-specific cytokine response across the Th1/Th2 spectrum. Increasing egg shedding was associated with body condition loss. However, local Trichuris-specific Th1/Th2 balance was positively associated with worm burden only in older wild mice. Thus, although the fundamental relationships between the CD4+ T helper cell response and resistance to T. muris infection are similar in both laboratory and wild M. m. domesticus, there are quantitative differences and age-specific effects that are analogous to human immune responses. These context-dependent immune responses demonstrate the fundamental importance of understanding the differences between model and natural systems for translating mechanistic models to 'real world' immune function.

Alternative splicing and environmental adaptation in wild house mice.

A major goal of evolutionary genetics is to understand the genetic and molecular mechanisms underlying adaptation. Previous work has established that changes in gene regulation may contribute to adaptive evolution, but most studies have focused on mRNA abundance and only a few studies have investigated the role of post-transcriptional processing. Here, we use a combination of exome sequences and short-read RNA-Seq data from wild house mice (Mus musculus domesticus) collected along a latitudinal transect in eastern North America to identify candidate genes for local adaptation through alternative splicing. First, we identified alternatively spliced transcripts that differ in frequency between mice from the northern-most and southern-most populations in this transect. We then identified the subset of these transcripts that exhibit clinal patterns of variation among all populations in the transect. Finally, we conducted association studies to identify cis-acting splicing quantitative trait loci (cis-sQTL), and we identified cis-sQTL that overlapped with previously ascertained targets of selection from genome scans. Together, these analyses identified a small set of alternatively spliced transcripts that may underlie environmental adaptation in house mice. Many of these genes have known phenotypes associated with body size, a trait that varies clinally in these populations. We observed no overlap between these genes and genes previously identified by changes in mRNA abundance, indicating that alternative splicing and changes in mRNA abundance may provide separate molecular mechanisms of adaptation.

Home-site advantage for host species-specific gut microbiota.

Mammalian species harbor compositionally distinct gut microbial communities, but the mechanisms that maintain specificity of symbionts to host species remain unclear. Here, we show that natural selection within house mice (Mus musculus domesticus) drives deterministic assembly of the house-mouse gut microbiota from mixtures of native and non-native microbiotas. Competing microbiotas from wild-derived lines of house mice and other mouse species (Mus and Peromyscus spp.) within germ-free wild-type (WT) and Rag1-knockout (Rag1-/-) house mice revealed widespread fitness advantages for native gut bacteria. Native bacterial lineages significantly outcompeted non-native lineages in both WT and Rag1-/- mice, indicating home-site advantage for native microbiota independent of host adaptive immunity. However, a minority of native Bacteriodetes and Firmicutes favored by selection in WT hosts were not favored or disfavored in Rag1-/- hosts, indicating that Rag1 mediates fitness advantages of these strains. This study demonstrates home-site advantage for native gut bacteria, consistent with local adaptation of gut microbiota to their mammalian species.

Two pup vocalization types are genetically and functionally separable in deer mice

Vocalization is a widespread social behavior in vertebrates that can affect fitness in the wild. Although many vocal behaviors are highly conserved, heritable features of specific vocalization types can vary both within and between species, raising the questions of why and how some vocal behaviors evolve. Here, using new computational tools to automatically detect and cluster vocalizations into distinct acoustic categories, we compare pup isolation calls across neonatal development in eight taxa of deer mice (genus Peromyscus) and compare them with laboratory mice (C57BL6/J strain) and free-living, wild house mice (Mus musculus domesticus). Whereas both Peromyscus and Mus pups produce ultrasonic vocalizations (USVs), Peromyscus pups also produce a second call type with acoustic features, temporal rhythms, and developmental trajectories that are distinct from those of USVs. In deer mice, these lower frequency "cries" are predominantly emitted in postnatal days one through nine, whereas USVs are primarily made after day 9. Using playback assays, we show that cries result in a more rapid approach by Peromyscus mothers than USVs, suggesting a role for cries in eliciting parental care early in neonatal development. Using a genetic cross between two sister species of deer mice exhibiting large, innate differences in the acoustic structure of cries and USVs, we find that variation in vocalization rate, duration, and pitch displays different degrees of genetic dominance and that cry and USV features can be uncoupled in second-generation hybrids. Taken together, this work shows that vocal behavior can evolve quickly between closely related rodent species in which vocalization types, likely serving distinct functions in communication, are controlled by distinct genetic loci.

Reemergence of Lymphocytic Choriomeningitis Mammarenavirus, Germany.

Lymphocytic choriomeningitis mammarenavirus (LCMV) is a globally distributed zoonotic pathogen transmitted by house mice (Mus musculus). We report the reemergence of LCMV (lineages I and II) in wild house mice (Mus musculus domesticus) and LCMV lineage I in a diseased golden lion tamarin (Leontopithecus rosalia) from a zoo in Germany.

The demographic history of house mice (Mus musculus domesticus) in eastern North America.

The Western European house mouse (Mus musculus domesticus) is a widespread human commensal that has recently been introduced to North America. Its introduction to the Americas is thought to have resulted from the transatlantic movements of Europeans that began in the early 16th century. To study the details of this colonization history, we examine population structure, explore relevant demographic models, and infer the timing of divergence among house mouse populations in the eastern United States using published exome sequences from five North American populations and two European populations. For North American populations of house mice, levels of nucleotide variation were lower, and low-frequency alleles were less common than for European populations. These patterns provide evidence of a mild bottleneck associated with the movement of house mice into North America. Several analyses revealed that one North American population is genetically admixed, which indicates at least two source populations from Europe were independently introduced to eastern North America. Estimated divergence times between North American and German populations ranged between ∼1,000 and 7,000 years ago and overlapped with the estimated divergence time between populations from Germany and France. Demographic models comparing different North American populations revealed that these populations diverged from each other mostly within the last 500 years, consistent with the timing of the arrival of Western European settlers to North America. Together, these results support a recent introduction of Western European house mice to eastern North America, highlighting the effects of human migration and colonization on the spread of an invasive human commensal.

Competitive ability is a fast-evolving trait between house mouse populations (Mus musculus domesticus).

House mice are commensal animals with a nearly global distribution, structured into well differentiated local populations. Besides genetic differences between the populations, they have also diverged behaviorally over time, whereby it remains open how fast general behavioral characteristics can change. Here we study the competitive potential of two very recently separated populations of the Western house mouse (Mus musculus domesticus) by using two different approaches-one under controlled cage conditions, the other under more natural conditions in enclosures mimicking a secondary encounter condition. We observe a clear bias in the competitive ability towards one of the populations for both tests. The measured behavioral bias is also reflected in the number of hybrid offspring produced in the enclosures. Our data suggest that key behavioral characteristics with a direct influence on relative fitness can quickly change during the evolution of populations. It seems possible that the colonization situation in Western Europe, with a rapid spread of the mice after their arrival, would have favored more competitive populations at the expansion front. The study shows the possible impact of behavioral changes on the evolution of populations.

Pathogen Detection in Ornithodoros sonrai Ticks and Invasive House Mice Mus musculus domesticus in Senegal.

Ornithodoros sonrai (O. sonrai) ticks are the only known vectors of Borrelia crocidurae, an agent of tick-borne relapsing fever (TBRF) borreliosis. Rodents serve as principal natural reservoirs for Borrelia. Our research objective was to detect TBRF Borrelia and other zoonotic bacterial infections in ticks and in house mice Mus musculus domesticus, an invasive species currently expanding in rural northern Senegal. Real-time and conventional PCR were utilized for detecting Borrelia and other bacterial taxa. The analyses were performed on 253 specimens of O. sonrai and 150 samples of brain and spleen tissue from rodents. Borrelia crocidurae was found in one O. sonrai tick and 18 Mus musculus domesticus samples, with prevalences of 0.39 percent and 12 percent, respectively, as well as Ehrlichia sp. in one Mus musculus domesticus. Further, we were able to detect the presence of a potentially infectious novel species belonging to the Anaplasmataceae family for the first time in O. sonrai ticks. More attention should be paid to the house mouse and O. sonrai ticks, as they can be potential hosts for novel species of pathogenic bacteria in humans.

Rodents in agriculture and public health in Malawi: Farmers’ knowledge, attitudes, and practices

Given that rodents are responsible for nearly 280 million cases of undernutrition worldwide and that about 400 million people are affected by rodent-associated zoonoses annually, management of rodent populations that are agricultural pests and/or reservoirs of pathogens is a major food security and public health matter. In sub-Saharan Africa, the median crop loss due to rodents is about 16% in the field and around 8% during storage. The impact on public health is not well-established, albeit over 60 zoonotic diseases can be spread to humans via rodents. Therefore, focusing on rodent-related community knowledge, attitudes, and practices is crucial to establishing robust baseline information as a springboard for future targeted studies. The study was conducted in September 2020 in Lilongwe and Nkhata Bay districts in Central and Northern Malawi, respectively. A semi-structured questionnaire, focus group discussions, and interviews with key informants were used. Farmers reported rodents were a major problem for staple crops (maize, rice, and cassava) and the main species responsible were the Natal multimammate mouse (Mastomys natalensis), silver mole-rat (Heliophobius argenteocinereus), and house mouse (Mus musculus domesticus). Awareness of rodent-associated health risks is very low, as exemplified by reports of rodent–human bites, eating rodent-contaminated food, and processing and consumption of wild rodents in poor hygienic conditions, and these practices were flourishing when Malawi was a bubonic plague endemic country. Rodent management is less practiced, and when practiced, it is symptomatic. It is considered a matter of individual households and typically relies on the use of rodenticides and insecticides without proper dosage and user instructions. We recommend rigorous campaigns to create better awareness among the public regarding the impacts of rodents on agriculture and community health and the need for community engagement for effective rodent management. A paradigm shift is needed by adapting and adopting practices of ecologically-based rodent management and reducing dependence on synthetic chemical rodenticides.

Age-related changes in microRNAs expression in cruciate ligaments of wild-stock house mice.

Cruciate ligaments (CL) of the knee joint are injured following trauma or aging. MicroRNAs (miRs) are potential therapeutic targets in musculoskeletal disorders, but there is little known about the role of miRs and their expression ligaments during aging. This study aimed to (1) identify if mice with normal physical activity, wild‐stock house mice are an appropriate model to study age‐related changes in the knee joint and (2) investigate the expression of miRs in aging murine cruciate ligaments. Knee joints were collected from 6 and 24 months old C57BL/6 and wild‐stock house mice (Mus musculus domesticus) for ligament and cartilage (OARSI) histological analysis. Expression of miR targets in CLs was determined in 6‐, 12‐, 24‐, and 30‐month‐old wild‐stock house mice, followed by the analysis of predicted mRNA target genes and Ingenuity Pathway Analysis. Higher CL and knee OARSI histological scores were found in 24‐month‐old wild‐stock house mice compared with 6‐ and 24‐month‐old C57BL/6 and 6‐month‐old wild‐stock house mice (p < 0.05). miR‐29a and miR‐34a were upregulated in 30‐month‐old wild‐stock house mice in comparison with 6‐, 12‐, and 24‐month‐old wild‐stock house mice (p < 0.05). Ingenuity Pathway Analysis on miR‐29a and 34a targets was associated with inflammation through interleukins, TGFβ and Notch genes, and p53 signaling. Collagen type I alpha 1 chain (COL1A1) correlated negatively with both miR‐29a (r = −0.35) and miR‐34a (r = −0.33). The findings of this study support wild‐stock house mice as an appropriate aging model for the murine knee joint. This study also indicated that miR‐29a and miR‐34a may be potential regulators of COL1A1 gene expression in murine CLs.

Prevalence of Cockroaches, Bed Bugs, and House Mice in Low-Income Housing and Evaluation of Baits for Monitoring House Mouse Infestations.

Pests are frequently found in homes, especially in apartment buildings in low-income communities. We investigated the prevalence and patterns of pest infestations in low-income communities in four cities (Jersey City, Linden, Paterson, Trenton) in New Jersey, USA. Resident interviews, visual inspections, and the placement of monitors were used to identify pest infestations. A total of 1,753 apartments from 19 buildings or building complexes were accessed. The infestation rates of cockroaches, bed bugs (Cimex lectularius L.), and house mice (Mus musculus domesticus Schwarz and Schwarz) were 37, 9, and 20%, respectively. Among apartments with cockroaches, 97.8, 2.5, and 0.8% had German cockroach [Blattella germanica (L.)], American cockroach [Periplaneta americana (L.)], and Oriental cockroach (Blatta orientalis L.), respectively. The percentage of residents who were aware of the presence of cockroaches, bed bugs, and house mice was 70.8, 55.3, and 56.8%, respectively. The prevalence of pest infestation was associated with resident ethnicity and gender. Among 856 interview responses, 78% implemented methods themselves to control pests in their homes in the past six months. Chocolate spread detected 99% of house mouse infestations and was much more sensitive than three commercial blank baits. Significant differences were also observed in the feeding preference of the three commercial blank baits.

The Contribution of Genetic and Environmental Effects to Bergmann's Rule and Allen's Rule in House Mice.

AbstractDistinguishing between genetic, environmental, and genotype × environment effects is central to understanding geographic variation in phenotypic clines. Two of the best-documented phenotypic clines are Bergmann's rule and Allen's rule, which describe larger body sizes and shortened extremities in colder climates, respectively. Although numerous studies have found inter- and intraspecific evidence for both ecogeographic patterns, we still have a poor understanding of the extent to which these patterns are driven by genetics, environment, or both. Here, we measured the genetic and environmental contributions to Bergmann's rule and Allen's rule across introduced populations of house mice (Mus musculus domesticus) in the Americas. First, we documented clines for body mass, tail length, and ear length in natural populations and found that these conform to both Bergmann's rule and Allen's rule. We then raised descendants of wild-caught mice in the lab and showed that these differences persisted in a common environment and are heritable, indicating that they have a genetic basis. Finally, using a full-sib design, we reared mice under warm and cold conditions. We found very little plasticity associated with body size, suggesting that Bergmann's rule has been shaped by strong directional selection in house mice. However, extremities showed considerable plasticity, as both tails and ears grew shorter in cold environments. These results indicate that adaptive phenotypic plasticity as well as genetic changes underlie major patterns of clinal variation in house mice and likely facilitated their rapid expansion into new environments across the Americas.

Interplay between historical and current features of the cityscape in shaping the genetic structure of the house mouse (Mus musculus domesticus) in Dakar (Senegal, West Africa)

Interplay between historical and current features of the cityscape in shaping the genetic structure of the house mouse (Mus musculus domesticus) in Dakar (Senegal, West Africa)

Steroid hormones in hair and fresh wounds reveal sex specific costs of reproductive engagement and reproductive success in wild house mice (Mus musculus domesticus)

Not only males but also females compete over reproduction. In a population of free-living house mice (Mus musculus domesticus), we analyzed how (metabolic) costs of aggressive interactions (reflected in fresh wounds and long-term corticosterone concentrations in hair) are predicted by individual reproductive physiology and reproductive success in males and females. Over eight years, we studied wounds and reproduction of more than 2800 adults under naturally varying environmental conditions and analyzed steroid hormones from more than 1000 hair samples. Hair corticosterone were higher and wounds more frequent in males than females. In males, wound occurrence increased with increasing breeding activity in the population, without affecting hair corticosterone levels. Unexpectedly, individual male reproductive success did not predict wounds, while hair corticosterone increased with increasing levels of hair testosterone and reproductive success. High corticosterone in hair of males might therefore reflect metabolic costs of fighting over reproduction. In females, hair corticosterone was generally lower than in males and high levels did not impede pregnancy. Reproductive investment (reflected in hair progesterone) was dissociated from reproductive success. Occasional wounds in females indicated individuals without recent reproductive success and revealed reproductive competition, presumably driven by instability in the social environment. In both sexes, corticosterone increased with age, but there was no evidence that received overt aggression, as indicated by wounds or elevated corticosterone, suppressed reproductive physiology. Our results diverge from laboratory findings and emphasize the need to also study animals in their natural environment in order to understand the complexity of their behavioral physiology.

The genomic basis of high-elevation adaptation in wild house mice (Mus musculus domesticus) from South America.

Understanding the genetic basis of environmental adaptation in natural populations is a central goal in evolutionary biology. The conditions at high elevation, particularly the low oxygen available in the ambient air, impose a significant and chronic environmental challenge to metabolically active animals with lowland ancestry. To understand the process of adaptation to these novel conditions and to assess the repeatability of evolution over short timescales, we examined the signature of selection from complete exome sequences of house mice (Mus musculus domesticus) sampled across two elevational transects in the Andes of South America. Using phylogenetic analysis, we show that house mice colonized high elevations independently in Ecuador and Bolivia. Overall, we found distinct responses to selection in each transect and largely nonoverlapping sets of candidate genes, consistent with the complex nature of traits that underlie adaptation to low oxygen availability (hypoxia) in other species. Nonetheless, we also identified a small subset of the genome that appears to be under parallel selection at the gene and SNP levels. In particular, three genes (Col22a1, Fgf14, and srGAP1) bore strong signatures of selection in both transects. Finally, we observed several patterns that were common to both transects, including an excess of derived alleles at high elevation, and a number of hypoxia-associated genes exhibiting a threshold effect, with a large allele frequency change only at the highest elevations. This threshold effect suggests that selection pressures may increase disproportionately at high elevations in mammals, consistent with observations of some high-elevation diseases in humans.

Same Invasion, Different Routes: Helminth Assemblages May Favor the Invasion Success of the House Mouse in Senegal.

Previous field-based studies have evidenced patterns in gastrointestinal helminth (GIH) assemblages of rodent communities that are consistent with “enemy release” and “spill-back” hypotheses, suggesting a role of parasites in the ongoing invasion success of the exotic house mouse (Mus musculus domesticus) in Senegal (West Africa). However, these findings came from a single invasion route, thus preventing to ascertain that they did not result from stochastic and/or selective processes that could differ across invasion pathways. In the present study, we investigated the distribution of rodent communities and their GIH assemblages in three distinct zones of Northern Senegal, which corresponded to independent house mouse invasion fronts. Our findings first showed an unexpectedly rapid spread of the house mouse, which reached even remote areas where native species would have been expected to dominate the rodent communities. They also strengthened previous insights suggesting a role of helminths in the invasion success of the house mouse, such as: (i) low infestation rates of invading mice by the exotic nematode Aspiculuris tetraptera at invasion fronts—except in a single zone where the establishment of the house mouse could be older than initially thought, which was consistent with the “enemy release” hypothesis; and (ii) higher infection rates by the local cestode Mathevotaenia symmetrica in native rodents with long co-existence history with invasive mice, bringing support to the “spill-back” hypothesis. Therefore, “enemy release” and “spill-back” mechanisms should be seriously considered when explaining the invasion success of the house mouse—provided further experimental works demonstrate that involved GIHs affect rodent fitness or exert selective pressures. Next steps should also include evolutionary, immunological, and behavioral perspectives to fully capture the complexity, causes and consequences of GIH variations along these invasion routes.

The effect of baculum shape and mating behavior on mating-induced prolactin release in female house mice

Abstract Male genitalia are subject to rapid divergent evolution, and sexual selection is believed to be responsible for this pattern of evolutionary divergence. Genital stimulation during copulation is an essential feature of sexual reproduction. In mammals, the male intromittent genitalia induces a cascade of physiological and neurological changes in females that promote pregnancy. Previous studies of the house mouse have shown that the shape of the baculum (penis bone) influences male reproductive success and responds to experimentally imposed variation in sexual selection. Here, we test the hypothesis that the baculum is subject to sexual selection due to a stimulatory function during copulation. We selected male and female house mice (Mus musculus domesticus) from families with breeding values at the extremes of baculum shape and performed two series of experimental matings following which we examined the concentration of prolactin in the blood of females either 15 (“early”) or 75 (“late”) min after ejaculation. Our results provide evidence of a mating-induced release of prolactin in the female house mouse early after ejaculation, the level of which is dependent on an interaction between the shape of the baculum and male sexual behavior. Our data thereby provide novel insight into the mechanism(s) of sexual selection acting on the mammalian baculum.

3D models related to the publication: Wild versus lab house mice: Effects of age, diet, and genetics on molar geometry and topography.

This contribution contains 3D models of upper molar rows of house mice (Mus musculus domesticus). The erupted parts of the right row and of the first upper molar are presented for specimens belonging to four groups: wild-trapped mice, wild-derived lab offspring, a typical laboratory strain (Swiss) and hybrids between wild-derived and Swiss mice. These models are analyzed in the following publication: Savriama et al 2021: Wild versus lab house mice: Effects of age, diet, and genetics on molar geometry and topography

The genomics of rapid climatic adaptation and parallel evolution in North American house mice.

Parallel changes in genotype and phenotype in response to similar selection pressures in different populations provide compelling evidence of adaptation. House mice (Mus musculus domesticus) have recently colonized North America and are found in a wide range of environments. Here we measure phenotypic and genotypic differentiation among house mice from five populations sampled across 21° of latitude in western North America, and we compare our results to a parallel latitudinal cline in eastern North America. First, we show that mice are genetically differentiated between transects, indicating that they have independently colonized similar environments in eastern and western North America. Next, we find genetically-based differences in body weight and nest building behavior between mice from the ends of the western transect which mirror differences seen in the eastern transect, demonstrating parallel phenotypic change. We then conduct genome-wide scans for selection and a genome-wide association study to identify targets of selection and candidate genes for body weight. We find some genomic signatures that are unique to each transect, indicating population-specific responses to selection. However, there is significant overlap between genes under selection in eastern and western house mouse transects, providing evidence of parallel genetic evolution in response to similar selection pressures across North America.

Molecular analysis of the blood meals and bacterial communities of bed bugs (Cimex lectularius L.) to assess interactions with alternative hosts.

Common bed bugs (Cimex lectularius L.) are hematophagous pests present in urban environments across the globe. It is widely established that they have a strong host preference for humans. However, there are records of C. lectularius feeding upon a range of mammalian and avian hosts, including rodents, in the field. There is little information available about how frequently common bed bugs feed on alternative hosts in residential settings, but understanding this phenomenon has implications for both management of infestations and public health. Here, we examined cohorts of C. lectularius collected from 13 different dwellings in the state of New Jersey, USA, that were known to be simultaneously infested with house mice (Mus musculus domesticus). Host-specific quantitative polymerase chain reaction (qPCR) was used to determine if blood meals were taken from mice, while 16S rRNA gene amplicon sequencing was used to screen the bed bugs for the presence of zoonotic bacterial pathogens. We found no evidence that any of the bed bugs we collected fed on mice. Furthermore, the insects harbored depauperate bacterial communities that did not include known human pathogens. However, host-specific qPCR detected feline DNA in a pool of bed bugs from one dwelling, suggesting that interaction with domestic pets should be further investigated. Although sampling in this study was limited, the approach described herein will be useful for additional studies of the interactions between bed bugs and alternative blood meal hosts.