PurposeTo identify priority areas and practice improvement strategies related to breastfeeding support, in a neonatal intensive care unit (NICU) in Colombia. MethodsAs part of a quality improvement initiative, this multi-method qualitative descriptive study involved 5 focus group interviews with NICU staff and 6 individual interviews with clinical leaders. Data were complemented by informal conversations with NICU staff and a document review of the hospital's breastfeeding policies and educational materials. Data were analyzed using qualitative content analysis. ResultsPriorities for practice improvement included (1) support for milk expression, (2) support for the transition to direct breastfeeding, and (3) creation of policies and tools related to breastfeeding. Findings highlighted ways in which resource limitations influence priority setting to favour the redistribution of existing assets and mobilization of local facilitators for breastfeeding support. ConclusionFindings will inform the development and implementation of future strategies to enhance breastfeeding support in this unit.