Worker Honeybees Apis Mellifera Research Articles

Effects of chronic oral exposure to insecticide teflubenzuron on the midgut of the honey bee Apis mellifera workers: histopathological insights into pesticide toxicity.

The honey bee Apis mellifera plays a significant role as a pollinator of native and cultivated plants, by increasing the productivity of several cultures, preserving the flora, and producing forest seeds. However, bee populations are declining worldwide, including A. mellifera, due to Colony Collapse Disorder, mainly resulting from the constant use of pesticides in the crops. Teflubenzuron is a physiological insecticide that belongs to the benzoylurea group, which inhibits chitin synthesis, the main component of the insect integument classified as safe for non-target insects, including bees. However, its effect on non-target organs of insects remains unknown. The midgut is the main organ of the digestive tract, which works in digestion and absorption and may be exposed to pesticides that contaminate food resources. The present work aimed to verify if the insecticide teflubenzuron is toxic and has histopathological effects on the midgut of A. mellifera adult workers. Workers exposed orally and chronically to the field-realistic concentration of teflubenzuron present 81.54% mortality. The epithelium of the midgut of these bees presents high vacuolization, spherocrystals, cell fragments released to the organ lumen, apocrine secretion, nuclear pyknosis, loss of cell-cell contact, and damage to regenerative cell nests and to the peritrophic matrix. These results indicate that the chitin synthesis-inhibiting insecticide teflubenzuron is toxic to A. mellifera after chronic oral exposure, at realistic field concentration, although it is classified as non-toxic to adult and non-target insects.

Acute oral exposure to imidacloprid induces apoptosis and autophagy in the midgut of honey bee Apis mellifera workers

The honey bee Apis mellifera is an important pollinator that increases the yield and quality of crops. In recent years, honey bee populations have declined in some parts of the world, which has been associated with several causes, including pesticides used in agriculture. Neonicotinoids are neurotoxic insecticides widely used in the world with systemic action mode contaminating nectar and pollen that may be consumed by bees. This study evaluated the side effects of imidacloprid in the midgut of A. mellifera after acute oral exposure. Toxicity, histopathology, cytotoxicity, and expression of autophagy-related gene atg1 were evaluated in honey bee workers orally exposed to imidacloprid. The estimated imidacloprid LC50 was 1.44 mg L−1. The midgut epithelium of bees fed on imidacloprid LC50 has the occurrence of cytoplasm vacuoles, enlarged intercellular spaces, disorganization of the striated border, and nuclear pyknosis, with an organ injury index that increases with time exposure. The midgut digestive cells of treated bees have apical protrusions, damaged mitochondria, and autophagosomes that were characterized for content with organelle debris and high expression of atg1. These features indicate the occurrence of high cell death in the midgut of workers exposed to imidacloprid, which may affect the digestibility the physiology of the insect.

Attractiveness of honey bee workers Apis mellifera L. to some food alternatives and their effect on increasing Honey bee colonies activity.

The experiment was carried out in the apiary of the College of Agricultural Engineering Sciences/University of Baghdad located in Al-Jadriya on hybrid only honey bee colonies Apis mellifera L. to find out the effect of nutrients (Matricaria chamomilla , coriandrum sativum, Foeniculum vulgare,Vicia faba, Dianthus chinensis) mixed with the bean powder on the attraction of honey bee workers outside the hives and added. It has the sugar solution on the area of honey, brood (closed and open) and pollen grains inside the hives, where the results showed that chamomile mixed with bean powder is more attractive to worker bees at a rate of 99.8 bees / hour, and less attractive with cloves with bean powder at a rate of 7.7 bees / hour, and that the evening feeding period More attractive to worker bees to food, at a rate of 69.9 bees per hour. The results showed that the rate of the effect of foodstuffs mixed with the sugar solution, as the treatment of chamomile with the sugar solution was higher than the rest of the treatments at a rate of 198.75 Eng2 at reading 3/6, then the rate gradually increased until it reached the highest rate at reading 10/6 and it was The treatment of chamomile with the sugar solution is superior to the rest of the treatments as it reached 257.5 Eng 2. Then the rate gradually decreased until it reached the lowest rate as it reached at the reading 6 / 24 in the treatment of chamomile with the sugar solution with the rest of the other treatments 187.67 Eng2.Corresponding author: E-mail (mohammed.shakir@coagri.uobaghdad.edu.iq) Al-Muthanna University All rights reserved

Fatty acids and elemental composition changes in honey bee workers (Apis mellifera) irradiated with gamma radiation

The effect of gamma radiation on the whole body contents of fatty acids and elemental composition were investigated for honey bee workers Apis mellifera. The data indicated that the quantities of fatty acids and elemental composition within the whole body of the workers of A. mellifera were affected significantly especially at the dose of 20 rad. Data obtained indicated that the palmitic (C16:0), oleic (C18:1) and linolic (C18:2) acids increased when workers irradiated with 20 rad. Analysis of the 7 elements; Mg, P, K, Ca, Fe, Cu and Zn were detected by energy dispersive X- ray analyzer by using (EDX). The most dominant elements in control workers were K followed by P; the Fe occupied the last order. In irradiated adult workers with 20 rad, K recorded a higher percentage than its control. In the same dose (20 rad), the total heavy metals Copper + Zink (Cu + Zn) decreased from 19.81% to 14.8%.

The Effect of An Alternative Diet Fermented by Bee Bread Microorganisms on Hypopharyngeal Glands Development and Acini Size of Honey Bee Workers, (Apis mellifera L.)

The hypopharyngeal glands of worker bees located in the head; consist of thousands of two-cell units that are composed of a secretory cell and a duct cell and that are arranged in sets of about 12 around a long collecting duct. The glands contribute to the production of the royal jelly fed to queens and larvae. They are highly sensitive to the quantity and quality of the food as pollen and pollen substitutes that the nurse bee consumes. The role of the worker honey bee Apis mellifera L. changes depending on age after eclosion (age polyethism): young workers (nurse bees) take care of their brood by synthesizing and secreting brood food (royal jelly), while older workers (foragers) forage for nectar and process it into honey. In our experiment, we tested how diets impact hypopharyngeal gland development and their acini size, where our diets compared were (bee bread diet; unfermented diet; fermented diet in a simulation method for nature; and sucrose syrup). Also, we mentioned understanding the role of these glands in hive health. For this study, we have examined the morphogenesis of the hypopharyngeal gland during different ages of workers honeybee Apis mellifera L. that fed on the different diets; we measured the size of glandular acini in a robust measure. These results obtained indicated that the hypopharyngeal gland development has flexibility and can depending on the condition of the colony as the pollen substitute diet we prepared in the periods of food shortage in nature. This described the hypothesis that feeding plays an important role in the development of HG according to diet nutritional values, ensuring the importance of the fermentation process for the better health of honey bees.

Stinging risk and sting pain of the ivy bee, Colletes hederae

The ivy bee, Colletes hederae, was first detected in Britain in 2001 and is abundant in many locations. Their ground nesting aggregations can occur in public areas and cause concern about stinging risk. Here we assess that risk. The likelihood of being stung or collided with when in an active aggregation was low, with only 1 sting in 10 h of activity (standing, walking, and “gardening”) by a human subject. Collisions were low at 6 per hour. The ivy bee sting is frequently unable to penetrate human skin. Only half were able to sting the fingertips and 75% the inner forearm when held against these areas. Pain was scored using a modified Starr/Schmidt scale. Ivy bee stings were significantly less painful than those of honey bee workers Apis mellifera, with similar scores to a nettle sting, Urtica dioica. Pain subsided after 10 min with weals smaller than both honey bees and nettles. The ivy bee venom sac was ca. 39% the volume of that of a worker honey bee. The sting is similar in size to the honey bee sting and barbs were not seen at 35× magnification. We conclude that the ivy bee is not a danger to the public. They rarely sting people active in a nest aggregation area, have a sting that is frequently unable to penetrate human skin and that causes minimal pain for a short duration, comparable to that of a nettle sting. Nettle stings are considered to be annoying but not dangerous.

Royal Jelly and Its Components Promote Healthy Aging and Longevity: From Animal Models to Humans.

Aging is a natural phenomenon that occurs in all living organisms. In humans, aging is associated with lowered overall functioning and increased mortality out of the risk for various age-related diseases. Hence, researchers are pushed to find effective natural interventions that can promote healthy aging and extend lifespan. Royal jelly (RJ) is a natural product that is fed to bee queens throughout their entire life. Thanks to RJ, bee queens enjoy an excellent reproductive function and lengthened lifespan compared with bee workers, despite the fact that they have the same genome. This review aimed to investigate the effect of RJ and/or its components on lifespan/healthspan in various species by evaluating the most relevant studies. Moreover, we briefly discussed the positive effects of RJ on health maintenance and age-related disorders in humans. Whenever possible, we explored the metabolic, molecular, and cellular mechanisms through which RJ can modulate age-related mechanisms to extend lifespan. RJ and its ingredients—proteins and their derivatives e.g., royalactin; lipids e.g., 10-hydroxydecenoic acid; and vitamins e.g., pantothenic acid—improved healthspan and extended lifespan in worker honeybees Apis mellifera, Drosophila Melanogaster flies, Gryllus bimaculatus crickets, silkworms, Caenorhabditis elegans nematodes, and mice. The longevity effect was attained via various mechanisms: downregulation of insulin-like growth factors and targeting of rapamycin, upregulation of the epidermal growth factor signaling, dietary restriction, and enhancement of antioxidative capacity. RJ and its protein and lipid ingredients have the potential to extend lifespan in various creatures and prevent senescence of human tissues in cell cultures. These findings pave the way to inventing specific RJ anti-aging drugs. However, much work is needed to understand the effect of RJ interactions with microbiome, diet, activity level, gender, and other genetic variation factors that affect healthspan and longevity.

Effects of coumaphos and imidacloprid on honey bee (Hymenoptera: Apidae) lifespan and antioxidant gene regulations in laboratory experiments

The main objective of this study was to test comparatively the effects of two common insecticides on honey bee Apis mellifera worker’s lifespan, food consumption, mortality, and expression of antioxidant genes. Newly emerged worker bees were exposed to organophosphate insecticide coumaphos, a neonicotinoid imidacloprid, and their mixtures. Toxicity tests were conducted along with bee midgut immunohistological TUNEL analyses. RT-qPCR assessed the regulation of 10 bee antioxidant genes linked to pesticide toxicity. We tested coumaphos at 92,600 ppb concentration, in combination with 5 and 20 ppb imidacloprid. Coumaphos induced significantly higher bee mortality, which was associated with down regulation of catalase compared to coumaphos and imidacloprid (5/20 ppb) mixtures, whereas, both imidacloprid concentrations independently had no effect on bee mortality. Mixture of coumaphos and imidacloprid reduced daily bee consumption of a control food patty to 10 mg from a coumaphos intake of 14.3 mg and 18.4 and 13.7 mg for imidacloprid (5 and 20) ppb, respectively. While coumaphos and imidacloprid mixtures induced down-regulation of antioxidant genes with noticeable midgut tissue damage, imidacloprid induced intensive gene up-regulations with less midgut apoptosis.

Measuring Hypopharyngeal Gland Acinus Size in Honey Bee (Apis mellifera) Workers.

The nurse hypopharyngeal glands produce the protein fraction of the worker and royal jelly that is fed to developing larvae and queens. These paired glands that are located in the head of the bee are highly sensitive to the quantity and quality of pollen and pollen substitutes that the nurse bee consumes. The glands get smaller when nurses are fed deficient diets and are large when they are fed complete diets. Because nurse hypopharyngeal gland size is a robust indicator of nurse nutrition, it is essential that those studying honey bee nutrition know how to measure these glands. Here, we provide detailed methods for dissecting, staining, imaging, and measuring nurse bee hypopharyngeal glands. We present comparisons of unstained and stained tissue and data that were used to study the impact of pollen on gland size. This method has been used to test how diet impacts hypopharyngeal gland size but has further use for understanding the role of these glands in hive health.

Draft Genome Sequences of Lactobacillus salivarius A3iob and Lactobacillus johnsonii CRL1647, Novel Potential Probiotic Strains for Honeybees (Apis mellifera L.).

This report describes the draft genome sequences of Lactobacillus salivarius A3iob and Lactobacillus johnsonii CRL1647, probiotic strains isolated from the gut of honeybee Apis mellifera workers. The reads were generated by a whole-genome sequencing (WGS) strategy on an Illumina MiSeq sequencer and were assembled into contigs with total sizes of 2,054,490 and 2,137,413 bp for the A3iob and CRL1647 strains, respectively. The draft genome sequences of L. salivarius A3iob and L. johnsonii CRL1647 will be useful for further studies of the specific genetic features of these strains and for understanding the mechanisms of their probiotic properties.

The Common Types of Pollen Grains Collected by Honey Bee Workers Apis Mellifera, L. (Hymenoptera:Apidae) in El-Sabheia Region, Alexandria Governorate, Egypt

The Common Types of Pollen Grains Collected by Honey Bee Workers Apis Mellifera, L. (Hymenoptera:Apidae) in El-Sabheia Region, Alexandria Governorate, Egypt

Effect of climate and radiation on the behavior of honey bee Apis mellifera workers

Effect of climate and radiation on the behavior of honey bee Apis mellifera workers

Morphological Changes and Colony Activity in Honeybee Workers Apis mellifera Produced from Irradiated Queens.

Morphological Changes and Colony Activity in Honeybee Workers Apis mellifera Produced from Irradiated Queens.

Reproductive traits and mandibular gland pheromone of anarchistic honey bee workers Apis mellifera occurring in China

In honey bee colonies, workers, in particular of “anarchistic” lineages, can activate their ovaries and lay eggs, even in the presence of the queen. We identified three queenright colonies showing typical signs of worker reproduction. To characterize this new lineage, we extracted the mandibular gland and analyzed it using gas chromatography. The total amounts of the five main components of the mandibular gland, namely methyl p-hydroxyben-zoate (HOB), 9-oxo-2(E)-decenoic acid (9-ODA), (S)-9-hydroxy-(E)-2-decenoic acid (9-HDA), 10-HDA, and 10-hydroxyde-canoic acid (10-HDAA) were significantly higher in the mandibular gland profiles of workers with activated ovaries (AWs, 8.88 ± 1.71 μg) compared to workers with inactivated ovaries (IAWs, 4.00 ± 2.09 μg). Furthermore, the chemical profiles of IAWs were dominated by the “worker substances” 10-HDA (34.64 ± 8.19 %) and its precursor 10-HDAA (22.88 ± 4.95 %), while the chemical profiles in AWs were dominated by the precursor of the queen substance 9-HDA (40.04 ± 7.55 %). The ratios of two precursor substances 10-HDAA/9-HDA of IAWs were more worker like (>1.0) whereas AWs were more queen like (≤1.0). These results suggest that the mandibular pheromones of anarchistic workers resemble a more queen-like reproductive active profile and that these workers may represent a reversion to a more basal reproductive phenotype.

Coumaphos affects food transfer between workers of honeybee Apis mellifera

We investigated whether coumaphos, an organophosphate used to control the parasitic mite Varroa destructor, affects trophallaxis in honeybees. Donors were treated with coumaphos 1 h before experiments. In this, uncontaminated food was transferred from donors to recipients. The amount of food transferred was measured (a) directly by weighing food or (b) indirectly by the number of recipients that died due to insufficient trophallaxis. A large reduction in the amount of transferred food was observed when the donors received 5 μg of coumaphos 1 h prior to the experiment. High mortality was observed among the recipients that were fed by donors treated with 2 or 5 μg of coumaphos indicating insufficient food transfer. Demonstrated side effects of coumaphos on trophallaxis could reduce distribution of this systemic agent in the colony and decreases its efficacy. The described assays are feasible and could be a useful tool to test the effects of pesticides on trophallaxis.

Effect of different feeding diets on the haemolymph of the newly emerged honeybee workers Apis mellifera L.

Five diets were compared for measuring their effects on the differential haemocytes types of the newly emerged worker bees, and also on their haemolymph proteins, lipids and glucose content. As blood haemocytes cell play a role in defending honeybees against parasites and pathogens. Five groups of newly emerged worker bees were fed with one of the following diets in patty form with sugar: faba bean (Vicia faba) pollen, maize (Zea mays) pollen, date palm (Phoenix dactylifera) pollen, Egyptian clover (Trifolium alexandrinum) pollen and soya bean (Glycine max) flour (as pollen supplement). Differential haemocyte counts (DHC) were evaluated in smears of their haemolymph. The types of the haemocytes (prohaemocytes, plasmatocytes, oenocytoids, granulocytes, coagulocytes and binucleated cells) were recorded. The most abundant type was plasmtoycte cells (over 90%) followed by granulocyte cells and coagulocyte cells. These blood cells perform phagocytosis and encapsulation of foreign bodies in the honeybee body cavity. Feeding honeybees with these different diets caused significant differences between the haemocyte cells. The highest number of plasmatocyte cells recorded in bees which fed on maize pollen, while the lowest was found in bees fed on bean pollen. The highest protein content was found in the haemolymph of bees fed on date palm and the lowest was found in bees fed on bean pollen and the difference was non-significant. The difference in lipids content was non-significant in worker bees fed on these different diets. There was a significant difference in glucose content between bees fed on maize pollen and date palm pollen.

Genome‐wide analysis of genes related to ovary activation in worker honey bees

A defining characteristic of eusocial animals is their division of labour into reproductive and nonreproductive specialists. Here, we used a microarray study to identify genes associated with functional sterility in the worker honey bee Apis mellifera. We contrasted gene expression in workers from a functionally sterile wild-type strain with that in a mutant (anarchist) strain selected for high rates of ovary activation. We identified a small set of genes from the brain (n = 7) and from the abdomen (n = 5) that are correlated in their expression with early stages of ovary activation. Sterile wild-type workers up-regulated two unknown genes and a homologue of Drosophila CG6004. By contrast, reproductive anarchist workers up-regulated genes for the yolk protein vitellogenin, venom peptides and a member of the AdoHycase superfamily, among others. The differentially expressed genes identified are likely to be involved in early differentiation into sterile and reproductive worker phenotypes and may therefore form part of the gene networks associated with the regulation of honey bee worker sterility. Our study may have lacked sufficient power to detect all but a minority of biologically relevant changes taking place; however, the differential expression of vitellogenin and a putative AdoHycase suggests that our screen has captured core reproductive genes and that ovary activation may involve an epigenetic mechanism.

Senescence in the worker honey bee Apis Mellifera

Honey bees are social insects that exhibit striking caste-specific differences in longevity. Queen honey bees live on average 1–2 years, whereas workers live 2–6 weeks in the summer and about 20 weeks in the winter. It is not clear whether queen–worker differences in longevity are due to intrinsic physiological differences in the rate of senescence, to differential exposure to extrinsic factors such as predation and adverse environmental conditions, or both. To determine if the relatively short lifespan of worker bees involves senescence, we measured age-specific resistance to three different physiological stressors (starvation, thermal, and oxidative stress) while eliminating age-related differences in foraging activity and minimizing age-related differences in energy expenditure. Despite these manipulations, older worker bees were still significantly less resistant to all three stressors than were younger bees. These results indicate that the regulation of worker bee lifespan involves senescence, in addition to extrinsic factors.

A hypopharyngeal gland protein of the worker honeybee Apis mellifera L. enhances proliferation of primary-cultured rat hepatocytes and suppresses apoptosis in the absence of serum

The worker honeybees of Apis mellifera L. age-dependently change from nurse bees which take care of their brood to forager bees which collect for nectar and convert it into honey during their lifespan of 30–40 days. A 56-kDa protein (p56kP-4) was shown to be present only in the nurse-bee hypopharyngeal gland. Here, we investigated the physiological effects of p56kP-4 on primary-cultured rat hepatocytes in the absence of serum. The p56kP-4 recombinant protein expressed in Escherichia coli significantly stimulated hepatocyte DNA synthesis and protected cells from apoptosis induced by serum deprivation. It also activated mitogen-activated protein kinase, as well as protein kinase B, which is a key regulator of cell survival. These findings suggest that p56kP-4 has a growth factor-like action on primary-cultured rat hepatocytes.

Pollination biology and breeding systems in the terrestrial orchid Bletilla striata

The levels of fruit set in different habitats (three inland populations vs. three coastal populations) and the breeding system in the terrestrial orchid Bletilla striata were examined in two groups of populations in Haenam-gun, Jollanam-do Province in southern Korea. In total inland populations (mean fruit set=13.3%) showed about eight-fold higher fruit set per plant than the coastal populations (1.6%), probably due to mild climatic conditions in the inland populations during the flowering period. Honey bee workers Apis mellifera were observed in the inland populations, but no visitors were found in the coastal populations. However, a great increase in fruit set was achieved by hand-pollination: the percentage of fruit set through artificial self-pollination and geitonogamous pollination were close to 90%, indicating that B. striata is self-compatible. Agamospermy and spontaneous autogamy (automatic self-pollination) were not detected in this study. These indicate that pollinia vectors are essential for achieving fruit set in natural populations of B. striata. Except in one inland population examined in 2001, there was no significant correlation between the patch size per population and the average percentage of fruit set, which might reflect deception-pollination of B. striata and/or paucity of pollinators. Overall levels of fruit sets within and among patches in each population were homogeneous during a two-year period.