In this paper, we study the polynomial integrability of natural Hamiltonian systems with two degrees of freedom having a homogeneous potential of degree k given either by a polynomial, or by an inverse of a polynomial. For k = − 2 , − 1 , … , 3 , 4 , their polynomial integrability has been characterized. Here, we have two main results. First, we characterize the polynomial integrability of those Hamiltonian systems with homogeneous potential of degree −3. Second, we extend a relation between the nontrivial eigenvalues of the Hessian of the potential calculated at a Darboux point to a family of Hamiltonian systems with potentials given by an inverse of a homogeneous polynomial. This relation was known for such Hamiltonian systems with homogeneous polynomial potentials. Finally, we present three open problems related with the polynomial integrability of Hamiltonian systems with a rational potential.