Homemade Extract Research Articles

Insecticidal potential of home-made neem extract against pod sucking bug, Clavigralla gibbosa (Spinola) in pigeonpea

Insecticidal potential of home-made neem extract against pod sucking bug, Clavigralla gibbosa (Spinola) in pigeonpea

Assessment of the anti-oxidant, anti-inflammatory and anti-bacterial activities of different types of turmeric (Curcuma longa) powder in Bangladesh

Turmeric (Curcuma longa) powder is one of the most popular spices in Bangladesh and it is widely available under different brands for consumers to choose from. The present study assessed the anti-oxidant, anti-inflammatory, and antibacterial activities of ethanolic extracts of turmeric powder from different forms (home-made, open-market and two commercially packed brands). The home-made and commercially packed (CP I) turmeric (Curcuma longa) powder extracts presented with all the tested phytochemical properties, yet saponin terpenoid and tannins were absent in the commercially packed (CP II) and open-market brands. The home-made turmeric powder had the largest amount (4.53 ± 0.41%) of curcumin compared to the others. Significant anti-oxidant activity was observed in the home-made sample compared to the others (IC50 = 126.78 ± 1.30 and 144.44 ± 3.47 μg/mL for H2O2 and DPPH scavenging activity). Furthermore the home-made turmeric powder exhibited significant (p<0.001) anti-inflammatory properties (IC50 = 114.73 ± 0.41 μg/mL). The most effective antibacterial activity was observed in home-made turmeric powder against Escherichia coli O157: H7 and Staphylococcus aureus NCTC 8325 with zone of inhibition ranged from 17.07 ± 0.27 mm and 17.03 ± 0.30 mm, respectively. Revealed in this study is the significant antioxidant, anti-inflammatory, and antimicrobial properties of all tested turmeric powder (CP I, CP II, open-market and home-made) extracts. The home-made extract showed the best potential. All the tested turmeric powders are good sources of spice for people to consume, but the home-made turmeric powder proved to be the best.

Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity

Pretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes. In the present work, an ultrasonic probe reaction system was used to evaluate the potential of swine hair pretreatment. The pretreated material was submerged with non-pretreated residues for 9 days to obtain the enzyme. Enzyme activity was measured in the extracts obtained using the ultrasonic probe, ultrasonic bath, and microwave. We also used the enzymatic concentration technique with NaCl and acetone. Homemade enzymatic extracts were evaluated for their ability to degrade swine hair and chicken feathers by comparing them with the activities commercial enzymes. Macrobeads gave greater energy dissipation in less time, providing greater enzyme activity (50.8 U/mL over 3 days). In terms of waste degradation, non-pretreated swine hair was more promising. The ultrasonic probe reaction system had the potential to evaluate increased enzyme activity (38.4% relative activity) and the enzyme concentration increased activity by 53.5%. The homemade enzymatic extract showed promise for degradation of keratinous residues.

ECONOMIC PROFITABILITY OF THE UTILIZATION OF HOMEMADE ORGANIC PESTICIDE FOR SMALL SCALE PRODUCTION OF Brassica Napus L. Varchinensis (PECHAY)

The study focused on the economic profitability of the utilization of homemade organic pesticide for small scale production of Brassica napus L. varchinensis (Pechay). An experiment was undertaken at the Palayamanan Program at the University of Eastern Philippines to determine the effectiveness of homemade organic pesticide in controlling or preventing insect infestation in Pechay, to find out the effective level of this homemade pesticide and to determine the economic profitability of using homemade organic pesticide and insecticide. To explore the use of homemade extract from garlic, Allium sativum L., hot pepper, Capsicum frutescens L., vinegar and mineral oil in the management of insect pests of Pechay. The design used was the randomized complete block design (RCBD) and the data gathered were statistically analyzed using ANOVA and DMRT at 5% level of significant. There were five treatments made up of 50% extracted organic insecticide diluted in 500ml and 1 litter of water treatment before the insects will attack (pre–treatment) in weekly intervals and separate application of the same formulation of the treatment after the insect will attack (post-treatment) two days interval and control without any treatment. The result of the study showed that those that gained higher profitable weight in treatment plots were the post – treatment.

Quantification through TLC-densitometric analysis, repellency and anticholinesterase activity of the homemade extract of Indian cloves.

The rise of the mosquitoes-transmitted diseases, like dengue, zika and chikungunya in Brazil in the last years has increased concerns on protection against mosquitoes bites. However, the prohibitive prices of the commercially available repellents for the majority of the Brazilian population has provoked a search for cheaper solutions, like the use of the homemade ethanolic extract of Indian clove (Syzygium aromaticum L.) as repellent, which has been reported as quite efficient by the local press. In order to verify this, we performed here the quantification of the main components of this extract through high-performance thin-layer chromatography (HPTLC)-densitometry and evaluated its efficiency as a repellent and its acetylcholinesterase(AChE) inhibition capacity. Our results have proved HPTLC-densitometry as an efficient and appropriate method for this quantification and confirmed the repellency activity, as well as its capacity of AChE inhibition.

Anaphylaxis to Wheat Flour-Derived Foodstuffs and the Lipid Transfer Protein Syndrome: A Potential Role of Wheat Lipid Transfer Protein Tri a 14

Background: Food allergy to wheat-derived foodstuffs is on the rise. Tri a 14, a wheat flour lipid transfer protein (LTP) allergen, has been described as a major allergen associated with baker’s asthma and wheat food allergy. Cross-reactivity among LTP allergens leads to the so-called ‘LTP syndrome’. Methods: Eight adult patients showing anaphylaxis after ingestion of wheat-derived foodstuffs were selected. A homemade wheat extract, purified natural (n) and recombinant (r) Tri a 14, and peach fruit and Artemisia pollen LTP allergens Pru p 3 and Art v 3 were subjected to skin prick test, specific IgE determination (ELISA) and IgE immunodetection assays. Results: All tests were positive in the 8 selected patients with the homemade extract. Positive skin prick test responses to nTri a 14, Pru p 3 and Art v 3 were found in 5/8, 6/8 and 4/4 patients, respectively. Specific IgE determined by ELISA assays was detected in 6 to nTri a 14 and rTri a 14, in 4 to Pru p 3 and in 3 to Art v 3 out of 8 individual sera tested, whereas all these sera showed IgE binding to nTri a 14 and Pru p 3 in immunodetection after SDS-PAGE separation. Conclusions: Tri a 14 seems to be a relevant allergen in patients with anaphylaxis after ingestion of wheat flour foodstuffs, according to in vitro and in vivo results. Clinical history of the analyzed patients, together with sensitization to peach Pru p 3 and Artemisia pollen Art v 3, suggests that 6 of them suffer from LTP syndrome.

Online RPLC-GC via TOTAD Method To Isolate (+)-Methyl Epijasmonate from Lemon (Citrus limon Burm.)

Pure (+)-methyl epijasmonate was isolated from lemon ( Citrus limon Burm.) for the first time. To that aim, two commercial essential oils and one homemade extract were included in the present paper. First, a study on the appropriate chromatographic conditions to avoid the epimerization from methyl epijasmonate to the more stable methyl jasmonate was accomplished. The results obtained are discussed. The presence of (+)-methyl epijasmonate in the three samples studied was initially established through the direct injection into GC-MS. However, the overlapping of (+)-methyl epijasmonate with other matrix components made it necessary to employ a multidimensional technique. RPLC-GC analysis via through-oven transfer adsorption-desorption (TOTAD) provided the selectivity and sensitivity required, reflecting that the homemade lemon extract was an adequate natural source to obtain pure (+)-methyl epijasmonate by means of the collection of the corresponding RPLC fraction.

Poisoning by lavandin extract in a 18-month-old boy

An 18-month-old boy ingested a small amount of homemade lavandin extract. The child developed a central nervous system depression and a confused state three hours after ingestion. The electroencephalogram showed fast rhythm disorders consistent with a toxic etiology. The outcome was favorable. Poisoning was confirmed by headspace-gas chromatography-mass spectrometry. Linalyl acetate, linalyl formate, and acetone were identified in pure lavandin extract and in the child's blood and urine. We report the only case of lavandin extract poisoning confirmed by toxicological analysis.

Prevention of the onset of hyperglycaemia by extracts of Aloe barbadensis in rabbits treated with alloxan

The ability of a home-made aqueous extract of Aloe barbadensis to prevent the onset of alloxaninduced hyperglycaemia was examined and compared with that of a factory-produced gel. Three groups of animals were administered 200 mg/kg body weight of alloxan intraperitoneally. A fourth group of animals was left uninjected. Animals in group 2 also received a uniform administration of 1 mg/ml twice daily oral intake of Aloe gel (the home-made extract) commenced at the same time the alloxan was administered and continued thereafter for the next six days. Animals in group 3 were administered the factory-produced extract in a similar way as was done for animals in group 2. Plasma glucose levels at the end of the experiment were 142.50±6.28, 82.50±2.72, 88.17±1.92 and 94.17±1.51mg/dl for animals in groups 1, 2, 3 and 4, respectively. Alloxan administration possibly resulted in an increase of 51% in the plasma glucose level of animals in group 1 relative to animals in group 4 (p