Holstein-Friesian Breed Research Articles

Digital technologies in dairy cattle breeding to improve the reproductive function of cows and heifers: A case study in Northern Kazakhstan

Background and Aim: In some countries, the application of digital technologies in dairy cattle breeding is still under development. This study aimed to investigate the use of digital technologies in dairy cattle breeding to improve the reproductive function of cows and heifers in three northern regions of Kazakhstan. Materials and Methods: This study explores the application of Austrian smaXtec bolus sensors, which enable the daily monitoring of the reproductive functions of cows and heifers in livestock. To control indicators of reproductive function in Simmental and Holstein-Friesian cattle breeds, a series of experiments were conducted before and after the introduction of boluses in the rumen. Results: It was established that the application of smaXtec boluses increases milk yield in 305 days, the percentage of conception in the first insemination and in cows with up to three inseminations, the duration of dry secretion, and the percentage of calve output per 100 heads. Moreover, the use of smaXtec boluses reduced the insemination index, duration of the calving-to-conception interval (open days), reproductive rate, and percentage of abortions and culls due to gynecological problems. Conclusion: The use of smaXtec boluses allows farmers and veterinarians to determine indicators, such as the period of sexual heat in livestock and diseases, in a timely manner and to increase the efficiency of feeding and controlling drinking cycles. Moreover, the application of smaXtec boluses minimizes labor costs associated with collecting data on indicators of reproductive function in cows and heifers and increases accuracy. Keywords: bolus, cows, digital technology, reproductive function, smaXtec.

Exposure of Cattle Breeding Herds to Naturally Co-Contaminated Zearalenone and Deoxynivalenol: The Relevance of a Urinary Mycotoxin Monitoring System for Herd Health and Food Safety.

The widespread presence of Fusarium mycotoxins in animal feed is a global issue, not only for the health of livestock but also for ensure the safety of food as an end product. High concentrations of zearalenone (ZEN) and deoxynivalenol (DON) have been detected in the diets of Japanese Black (JB) and Holstein Friesian (HF) breeding herds. Consequently, we monitored serum biochemical parameters over a long time in both herds, focusing on anti-Müllerian hormone (AMH) levels and acute-phase inflammation. Additionally, urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) and progesterone levels were measured in the HF herd. The JB herd, a ZEN-dominant model with low DON contamination, demonstrated ZEN levels that exceeded the Japanese limit in the purchased total mixed rations (TMR). Conversely, the HF herd, which primary consumes DON-dominant feed with low ZEN contamination, had high DON levels in the dent corn silage. Specifically, the JB herd's TMR contained 1.79 mg/kg ZEN and 0.58 mg/kg DON, whereas the HF herd's silage had 15.3 mg/kg DON (dried sample) and 0.1 mg/kg ZEN. Enzyme-linked immunoassay were used to measure urinary ZEN-DON levels following confirmation through liquid chromatography-tandem mass spectrometry. Urinary ZEN-DON levels measured were significantly correlated (p < 0.05, r > 0.6) in both herds. In the HF herd, AMH levels increased (p = 0.01) and serum amyloid A (SAA) levels decreased (p = 0.02) when contaminated and at the end of the monitoring period. Additionally, urinary ZEN and DON levels were significantly correlated with SAA levels (ZEN: p = 0.00, r = 0.46; DON: p = 0.03, r = 0.33), with an increase in ZEN and DON levels resulting in higher SAA levels. The JB herd showed no significant differences. Additionally, in the HF herd, 8-OHdG/Cre levels increased significantly during major contamination periods (p < 0.05). Clinical data from the HF herd indicated an increase in mastitis cases and treatment rates during periods of major contamination. Abortion rates in the HF herd decreased from 22.9% (before monitoring) to 8.9% (during the high contamination period) and finally to 1% (at the end of the monitoring period), with corresponding increases in progesterone levels. ZEN-DON contamination adversely affects breeding cattle's productivity, reproductive performance, and health. Therefore, monitoring urinary ZEN-DON is valuable for detecting contaminants and ensuring the safety of food products.

Analysis of Inflammatory and Regulatory Cytokines in the Milk of Dairy Cows with Mastitis: A Comparative Study with Healthy Animals

ABSTRACT Bovine mastitis remains a major problem in the global dairy cattle industry. The acute invasion of udder by pathogens induces innate immune response as the first defence mechanism in subclinical and clinical mastitis. The aim of the study was to determine inflammatory and regulatory cytokines IL-2, IL-4, TGF-β1, IL-17A, beta-defensin 3 and IL-10 and their potential changes in milk of dairy cows with subclinical and clinical mastitis, and to compare the findings with healthy animals. Milk samples from 15 holstein Friesian breed cows were used in the study. Cows were divided into three groups based on their health status (5 healthy, 5 subclinical and 5 clinical animals). All samples were tested using immunohistochemistry to evaluate IL-2, IL-4, IL-10, IL17A, TGF-β1 and β-Def 3 proteins. Expression of all proteins was detected in all milk samples. High expression of IL-2, IL-4, IL17A, TGF-β1 was detected in healthy cows’ milk and in milk of cows with subclinical and clinical mastitis. However, expression of IL-10 and β-Def 3 in milk samples of healthy cows was significantly higher compared to the milk of cows with subclinical and clinical mastitis (p < .001). IL-10 and β-Def 3 can be considered as informative biomarkers in diagnosis of subclinical and clinical mastitis.

The Impact of Varying Pasture Levels on the Metabolomic Profile of Bovine Ruminal Fluid.

A pasture or concentrate-based dietary regime impacts a variety of factors including both ruminal health and function, and consequently milk production and quality. The objective of this study was to examine the effect of feeding differing pasture levels on the metabolite composition of bovine ruminal fluid. Ruminal fluid was obtained from rumen-cannulated spring-calving cows (N = 9, Holstein-Friesian breed, average lactation number = 5) fed one of three diets across a full lactation season. Group 1 (pasture) consumed perennial ryegrass supplemented with 5% concentrates; group 2 received a total mixed ration (TMR) diet; and group 3 received a partial mixed ration (PMR) diet which included pasture and a TMR. Samples were taken at two timepoints: morning and evening. Metabolomic analysis was performed using nuclear magnetic resonance (1H-NMR) spectroscopy. Statistical analysis revealed significant changes across the dietary regimes in both morning and evening samples, with distinct alterations in the metabolite composition of ruminal fluid from pasture-fed cows (FDR-adjusted p-value < 0.05). Acetate and butyrate were significantly higher in samples derived from a pasture-based diet whereas sugar-related metabolites were higher in concentrate-based samples. Furthermore, a distinct diurnal impact on the metabolite profile was evident. This work lays the foundation for understanding the complex interaction between dietary regime and ruminal health.

The Influence of Selected Environmental Factors on the Number of Somatic Cells in Cistern and Alveolar Milk of Polish Holstein-Friesian Cows.

The aim of the study was to evaluate the influence of the milking phase on somatic cell count (SCC) in milk obtained from the cisternal and alveolar parts of udders of selected Polish Holstein-Friesian cows. The study also assessed the impact of other genetic and environmental factors on SCC variability in cisternal and alveolar milk, including: the individual cow, lactation stage, age of cow, production level, milking speed, fat-to-protein ratio, and milking type. The research included 15 cows of Polish Holstein-Friesian breed at different ages, lactation stages, and with varying daily milk yield. A total of 210 milk observations were conducted, including 105 for 1 min milking and 105 for 8 min milking. The results obtained in the study indicated that milk obtained during two different milking phases exhibited similar SCC levels (F for LOGSCC = 0.79). The average actual SCC in milk produced by 15 cows in 105 observations for 1 min milking was 219,000 cells/mL, while for 8 min milking it was 229,000 cells/mL. The results were inconclusive, suggesting that SCC in cisternal and alveolar milk must be influenced by factors other than the milking phase. The analysis of variance conducted for this purpose provided the basis for stating a highly statistically significant effect of the individual cow (F for LOGSCC = 147.9), lactation stage (F for LOGSCC = 54.64), age of cow (F for LOGSCC = 12.39), daily production level (F for LOGSCC = 34.49), milking speed (F for LOGSCC = 17.56), and fat-to-protein ratio (F for LOGSCC = 22.99) on the variability of characteristics defining SCC in milk. In summary, SCC is characterized by high variability, influenced by a range of environmental and genetic factors such as the individual cow, lactation stage, age of cow, milking speed, and dietary fat-to-protein ratio. The influence of milking phase (1 min or 8 min) and milking type (morning or evening) should be considered inconclusive based on the entire population studied. For half of the cows, SCC in cisternal milk was higher than in alveolar milk, while for the other half, the situation was reversed. Further observations are required to confirm the hypothesis regarding the extent to which cows' immunological response to bacterial infections is concentrated in the cisternal or alveolar part of the udder under national environmental conditions.

Gene Expression Level and Immunohistochemical Localization of Cannabinoid and Cannabinoid-Related Receptors in The Small Intestine of Holstein Bulls (Bos Taurus Taurus)

Abstract The gastrointestinal tract plays a crucial role in nutrient absorption, secretion, and motility, ensuring proper digestion and overall homeostasis. Regulation of this complex system involves the coordination of various communication pathways, including neural and humoral mechanisms. One such mechanism is the endocannabinoid system (ECS), a signaling network comprising endogenous cannabinoids, receptors, and enzymes involved in the regulation of physiological processes in mammals and non-mammalian species. While extensive research has been conducted on the ECS in monogastric animals, limited information is available on its presence and distribution in cattle. This study aimed to investigate the distribution and localization patterns of cannabinoid receptors type 1 (CB1R) and type 2 (CB2R) and transient receptor potential vanilloid type 1 (TRPV1) in the bovine small intestine. The study included immunohistochemical analysis of intestinal tissue samples from Polish Holstein-Friesian breed bulls. Gene expression levels of CNR1, CNR2, and TRPV1 genes, encoding CB1R, CB2R, and TRPV1, respectively, were quantified using qPCR analysis. The results showed that all three receptors were expressed in the bovine small intestine, with TRPV1 exhibiting a significant upregulation in the jejunum compared to the duodenum and ileum. Immunoreactivity for CB1R and CB2R was predominantly observed in neurons of the enteric plexuses, while TRPV1 immunolabeling was detected in both enteric neurons and duodenal Brunner’s glands. These findings may establish an anatomical foundation for further investigations, lending support to the potential therapeutic efficacy of cannabinoid receptor agonists in alleviating gastrointestinal motility disorders associated with bovine enteropathies and optimizing milk production in dairy cattle.

The Effectiveness of Using Bulls With Increased Immune Response in Terms of Milk Yield, Milk Contents, and Somatic Cell Count in Polish Holstein-Friesian Cows

Abstract The dairy cattle industry has witnessed significant advancements in production traits, including milk yield, reproduction, and health, through decades of breeding efforts. Despite these improvements, cow health and milk hygiene remain essential. High immune response (HIR) technology identifies cattle with optimized immune responses, enabling the selection of bulls with high immune responses for breeding. Studies indicate that HIR-based breeding improves animal health without compromising production traits. This study aimed to assess the effectiveness of bulls with a high immune response in improving milk yield, milk contents, and somatic cell count in the milk of Polish Holstein-Friesian cows. A total of 11,509 cows of the Polish Holstein-Friesian breed were used. Among those, 1,773 animals were the offspring of HIR bulls, including 926 first-generation offspring (called I50 group) and 847 second-generation offspring (I25 group). There were 9,736 animals unrelated to HIR bulls (I0 group). Selected production and functional traits were analyzed. The results show that cows with higher kinship to HIR bulls showed increased milk yield. However, their milk exhibited lower fat, protein, and casein content. A favorable impact of HIR bulls was demonstrated by the decrease in somatic cell count in their offspring’s milk compared to the milk of cows from the I0 group. This research contributes valuable insights into the potential benefits of using HIR bulls in dairy cattle breeding programs, emphasizing the importance of balancing production traits with health considerations.

WPŁYW GENOTYPU I CZASU DOJRZEWANIA NA CECHY FIZYKOCHEMICZNE MI ˛ESNI ´ SZKIELETOWYCH BUHAJKÓW OPASANYCH W SYSTEMIE PÓŁINTENSYWNYM

The aim of the study was to determine the effect of the genotype and ageing time on pH, water holding capacity, shear force and energy, and the myofibrillar index of m. longissimus lumborum and m. semitendinosus muscles in semi-intensively fed bulls. Carcass value indices and the chemical composition of the muscles depending on the genetic group were assessed as well. The study involved 46 young bulls from four genetic groups, i.e. the Hereford breed (HER, 8 bulls), the Limousin breed (LIM, 8 bulls), commercial cross-breeds (MT, 14 bulls), and the Polish Black-and-White Holstein-Friesian breed (PHF, 16 bulls). The analyses showed that the semi-intensive fattening system ensures production of carcasses with satisfactory slaughter yield and quality parameters (good musculature and moderate fatness). The best quality parameters were determined in the meat from the LIM and MT young bulls, whereas the poorest results were exhibited by the PHF beef. The ageing time had a positive effect on the meat quality parameters (especially tenderness and color), contributing to a significant reduction in the differences between the genotypes observed in the initial postmortem period. The results of the shear force and myofibrillar index, i.e. parameters of meat tenderness, suggest that a 14-day ageing period seems to be the necessary minimum. This period should even be extended in the case of the LL muscle of HER and PHF young bulls or the ST muscle of HER, MT, and PHF young bulls. Further research with consumer assessment is therefore advisable to determine the optimal ageing time of beef produced in the country.

Some Environmental Factors Causing First Calving Difficulties in Holstein Friesian Cattle

Calving efficiency, an important target in cattle breeding, has been negatively affected by some environmental factors. Therefore, calving difficulty creates negative economic consequences. In this study, it was aimed to investigate the relationship between the first calving difficulty in Holstein Friesian (HF) cows in terms of management and origin factors. The material of the research consists of 1475 calving difficulty records from 5 different enterprises engaged in HF breeding, located in the Central Anatolia Region of Turkey, covering the years from 2013 to 2019. The scoring system used to determine calving difficulty: normal without intervention (ND), normal with intervention (MND), difficult/intervention with equipment (ZD), and abnormal birth (AD). Chi-square tests were performed. In the calving difficulty analysis, the management factor is classified as 1-2-3-4-5 and the origin of the cow is classified as 1 (foreign origin) and 2 (native origin). No findings were observed for MND scores. Total ND, ZD, and AD scores were 1250 (84.74%), 192 (13.01), and 33 (2.25%), respectively. Chi-square test was performed to test the differences among farms. Among the enterprises, the highest ND rate was observed in the 5th enterprise with 90.07%, the minimum ZD rate was observed in the 5th enterprise with 8.45%, and the AD rate was at least 0.66% in the 1st enterprise. The difference between farms was significant for calving difficulty (P

A retrospective case series of the surgical management of thirty-one penile fibropapilloma cases presented to University College Dublin Veterinary Hospital (UCDVH) between 2017 and 2023

BackgroundPenile fibropapilloma is a condition caused by bovine papilloma virus and is frequently encountered in young bulls. Penile fibropapillomatosis is thought to be spread through homosexual mounting behaviour. Fibropapillomas of the penis are painful, often bleed and can impede normal intromission. Treatment may range from allowing time for slow, spontaneous regression to surgical resection but recurrence following surgery is reported by some authors.Case PresentationThirty one bulls that were presented to University College Dublin Veterinary Hospital from March 2017 to March 2023 for surgical resection of penile fibropapillomas were included in this retrospective case series. Twenty-seven of the 31 bulls (87%) were under two years of age. The majority (42%) of bulls presented were Hereford, but Angus, Charolais, Holstein-Friesian and Limousin breeds were also seen. Following examination and diagnosis of penile fibropapilloma, regional anaesthesia (xylazine-procaine epidural and internal pudendal nerve block) and standing surgical intervention (resection and cautery) was performed in each case. Phone call follow-up was performed by one author (EET) in all 31 cases and 2 cases out of the 28 that were contactable showed post-surgical recurrence of penile fibropapillomatosis (i.e., 7.1% recurrence rate).ConclusionThis case series summarises the history and presenting findings of 31 bovine penile fibropapilloma cases and describes a regional anaesthetic and standing surgical approach for successful case management.

Prevalence and analysis of risk factors of udder and teat health disorders in free-grazing and zero-grazing small holder dairy cows in south wollo, Ethiopia

Udder and teat health disorders (UTHDs) cause profound economic loss and have a major influence on dairy cows’ welfare and productivity. This study was carried out to identify the prevalence and risk factors of UTHDs and to investigate a potential association with clinical mastitis (CM). The prevalence of UTHDs in this study was 28.4%. The UTHDs identified were CM (15.1%), teat ulcer/cracks (7.6%), udder wound (7.3%), teat end lesions (3.1%), teat papilloma (2.6%), pendulous udder (2.3%), hyperthelia (1.6%), athelia (1.0%), udder abscess (1.0%), teat stenosis (0.8%), teat gangrene (0.8%) and udder impetigo (0.8%). A significantly higher prevalence of UTHDs was identified in Holstein-Friesian (HF) cross breeds (P &lt; 0.001; OR = 0.134) than indigenous breeds; in cows between 4-5 years of age (P &lt; 0.001; OR = 11.666) compared to &gt; 7 years; in cows with good BCS (P = 0.007; OR = 0.263) than those with poor BCS and in milking (P &lt; 0.001; OR = 4.682) and dry cows (P = 0.003; OR = 4.226) than pregnant cows. Prevalence did not differ significantly (P &gt; 0.05) between the locations and management systems of herds. In addition, the occurrence of CM was associated with teat ulcer/cracks (P &lt; 0.001; OR = 0.036), udder wound (P &lt; 0.001; OR = 0.068); teat end lesions (P = 0.008; OR = 0.142); pendulous udder (P = 0.031; OR = 0.153) and udder impetigo (P = 0.040; OR = 0.058). A statistically insignificant difference was found in the prevalence of UTHDs between free-grazing and zero-grazing smallholder dairy herds. The study identifies teat ulcers/cracks, udder wounds, and teat end lesions as risk factors for CM.

Heritability and genetic correlations of rumination time with milk-yield and milking traits in Holstein-Friesian cows using an automated milking system

Knowledge of the values of genetic parameters is a prerequisite for conducting a breeding program. This is especially important for rumination, which is considered an indicator of cow’s health. Exploring the genetic relations between rumination time, milk yield, and milking traits could make it a valuable tool in dairy cattle breeding strategies. The objective of the research was to estimate heritability, repeatability, and genetic and phenotypic correlations of rumination time (RT), as well as traits associated with milk yield and milking of dairy cows of the Polish Holstein-Friesian breed kept in herds equipped with an automatic milking system. The research takes into consideration daily results for milking in the first lactation and second lactation, from 1 486 cows of the breed milked between 2013 and 2015 year. Cows were housed in 24 free-stall barns and fed a Partial Mixed Ration feed. The barns had an automated milking system (Astronaut A4 – Lely Industry). The cows received a varied dose of the concentrate, either in the milking robot or the feeding station, depending on the level of their milk yield. Our research has shown that RT was a low heritable trait (0.140 ± 0.039) and had a medium repeatability (0.572 ± 0.007). We detected a positive genetic correlation between RT and milk yield (0.341); however, a statistically significant negative relationship was identified between RT and urea content (−0.418) in milk. Estimations of genetic correlations suggest that selecting for higher RT may correspond to reduced urea content in milk. Investigating the genetics aspect of RT and the relationship with milk yield and milking traits may turn this into one of the useful criterion selections for dairy cattle breeding strategies, but should be used carefully. Further analyses on larger data sets and different populations are necessary.

New Implications of Metabolites and Free Fatty Acids in Quality Control of Crossbred Wagyu Beef during Wet Aging Cold Storage.

Efficient cold-chain delivery is essential for maintaining a sustainable global food supply. This study used metabolomic analysis to examine meat quality changes during the "wet aging" of crossbred Wagyu beef during cold storage. The longissimus thoracic (Loin) and adductor muscles (Round) of hybrid Wagyu beef, a cross between the Japanese Black and Holstein-Friesian breeds, were packaged in vacuum film and refrigerated for up to 40 days. Sensory evaluation indicated an increase in the umami and kokumi taste owing to wet aging. Comprehensive analysis using gas chromatography-mass spectrometry identified metabolite changes during wet aging. In the Loin, 94 metabolites increased, and 24 decreased; in the Round, 91 increased and 18 decreased. Metabolites contributing to the umami taste of the meat showed different profiles during wet aging. Glutamic acid increased in a cold storage-dependent manner, whereas creatinine and inosinic acid degraded rapidly even during cold storage. In terms of lipids, wet aging led to an increase in free fatty acids. In particular, linoleic acid, a polyunsaturated fatty acid, increased significantly among the free fatty acids. These results provide new insight into the effects of wet aging on Wagyu-type beef, emphasizing the role of free amino acids, organic acids, and free fatty acids generated during cold storage.

Expression of Genes Encoding Selected Orexigenic and Anorexigenic Peptides and Their Receptors in the Organs of the Gastrointestinal Tract of Calves and Adult Domestic Cattle (Bos taurus taurus).

The regulation of food intake occurs at multiple levels, and two of the components of this process are orexigenic and anorexigenic peptides, which stimulate or inhibit appetite, respectively. The study of the function of these compounds in domestic cattle is essential for production efficiency, animal welfare, and health, as well as for economic benefits, environmental protection, and the contribution to a better understanding of physiological aspects that can be applied to other species. In this study, the real-time PCR method was utilized to determine the expression levels of GHRL, GHSR, SMIM20, GPR173, LEP, LEPR, and NUCB2 (which encode ghrelin, its receptor, phoenixin-14, its receptor, leptin, its receptor, and nesfatin-1, respectively) in the gastrointestinal tract (GIT) of Polish Holstein-Friesian breed cattle. In all analyzed GIT segments, mRNA for all the genes was present in both age groups, confirming their significance in these tissues. Gene expression levels varied distinctly across different GIT segments and between young and mature subjects. The differences between calves and adults were particularly pronounced in areas such as the forestomachs, ileum, and jejunum, indicating potential changes in peptides regulating food intake based on the developmental phase. In mature individuals, the forestomachs predominantly displayed an increase in GHRL expression, while the intestines had elevated levels of GHSR, GPR173, LEP, and NUCB2. In contrast, the forestomachs in calves showed upregulated expressions of LEP, LEPR, and NUCB2, highlighting the potential importance of peptides from these genes in bovine forestomach development.

A Research on Fertility, Herd Life, Milk Production and Milk Quality Characteristics of Simmental (Fleckvieh) Cows: 2. Milk Quality

The aim of this study was to determine the milk quality characteristics of Simmental (SIM) cows of Austrian origin, which have increased the interest of breeders in Türkiye in recent years. For this aim, the milk analysis results of a farm located in Menemen County, İzmir/Türkiye from 2012 to 2021 were used. Milk fat (MF, %), protein (MP, %), lactose (ML, %), total dry matter (TDM, %) contents and somatic cell count (SCC, cell/ml) were determined. In order to determine the current situation, milk samples were taken from the cows (90 heads) in August 2021, and in addition to the above milk components, the solid non-fat (SNF) and freezing point (FP) were determined. The effects of sampling season, calving month, lactation month, sampling season x calving month and sampling season x lactation month interactions were found to be statistically significant for all traits (P&lt;0.05). Parity and calving month effects on Log10SCC were also detected to be statistically significant (P&lt;0.05). The mean MF, MP, ML, TDM, FP and SCC of SIM cattle were 3.71±0.018%, 3.42±0.009%, 4.63±0.009%, 12.49±0.03, -0.535±0.003oC and 5.14±0.01 (138.038 cells/ml), respectively. It was concluded that the milk components of Austrian-origin SIM cattle are not very different from the Holstein-Friesian (HF) breed, however, in the low SCC average for many years, besides the important contribution of the measures taken against mastitis in the farm, the resistance against mastitis may be higher in this genotype. This situation is thought to be the reason why breeders in Türkiye prefer Austrian-origin SIM cattle in addition to high milk yield and carcass weight.

The past and present of breeding in dairy cattle breeding in the Far North-East

Based on historical facts and past achievements, the work is designed to update the importance and prove the possibility of successful breeding work at the regional level in order to increase the production of high-quality, socially significant food products to ensure food security. At the beginning of the formation of agricultural science in the Magadan region, scientists were faced with the task of selecting the optimal breeds for the region. More than 4000 cows were examined during 1964–1967. It was found that in extreme natural and climatic conditions of the Magadan region the Kholmogory breed proved to be the best one. In 1967, in order to further improve the Kholmogory breed, the first attempt was made to study the genealogical structure of the herd, to identify and evaluate highly productive lines. In 1974, in order to increase the milk fat content of crossbred cows of the Kholmogory breed, introductory crossing with the bulls of the Ayrshire breed, tested for the quality of the offspring, was used. In order to improve dairy herds, the selection of cows for hereditary traits is combined with checking them for the first lactation. Since 1976, state farms began to study the breeding and productive qualities of the Ayrshire cattle, which were distinguished by high milk production and high fat content in milk. Since 1982, the Ayrshire breed has been approved as a planned breed for breeding in the farms of the Okhotsk zone. In the same year, absorptive crossbreeding of the animals of the Kholmogory breed with the sires of the Ayrshire breed began. In order to increase the efficiency of breeding for abundant milk production, work began on the farms to increase the hereditary variability of this trait through breeding along lines and families. At the same time, the work on improving the Kholmogory cattle by introductory crossbreeding with the purebred bulls of the Holstein-Friesian breed continued. By the 1990s, the main vector of development was the creation of highly productive herds of dairy cattle, but the difficult financial situation at farms in the 1990s led to a sharp decrease in the number of cattle and the productivity of the remaining animals.

WPŁYW RODZAJU PREPARATU MLEKOZAST ˛EPCZEGO NA WYNIKI ODCHOWU CIEL ˛AT ORAZ WSKAZNIKI HEMATOLOGICZNE I BIOCHEMICZNE KRWI

The aim of this study was to evaluate the effect of the type of milk replacer on the production results of calf rearing and hematological and biochemical parameters of their blood. Calves (heifers) of the Polish Holstein-Friesian breed, divided into two groups of 18 animals each, were maintained and fed equally, except for milk replacers used from the 31st to the 90th day of calf life. Group A received milk replacer based on skimmed milk powder, and group B received milk replacer based on whey powder. Production parameters were calculated and hematological and biochemical blood indices were determined. Better calf rearing results were found when the formula A was used. Calves, despite a lower initial weight at 30 days of age, reached a higher final weight at 90 days of rearing. There was a significantly higher number of leukocytes and erythrocytes in the blood of gorup A clves, whereas in group B there was a higher average red blood cell volume and average weight of hemoglobin in a red blood cell. In addition, there were significant differences in the levels of total cholesterol and HDL cholesterol in the blood of calves fed different milk replacers (P ≤ 0.05).

Low proviral load in the Kumamoto strain of Japanese Brown cattle infected with the bovine leukemia virus

BackgroundThe Kumamoto strain of Japanese Brown (JBRK) cattle is a sub-breed of Wagyu and has a different genetic background than that of Japanese Black (JB) cattle. Bovine leukemia virus (BLV) is the pathogen causing enzootic bovine leukosis (EBL), the predominant type of bovine leukosis (BL). EBL is one of the most common bovine infectious diseases in dairy countries, including Japan. Some host genetic factors, including the bovine leukocyte antigen (BoLA)-DRB3 gene, have been associated with the proviral load (PVL) of BLV and/or onset of EBL. Here, we determined the number of BL cases by analyzing prefectural case records in detail. We measured the PVL of BLV-infected JBRK cattle and compared it with that obtained for other major breeds, JB and Holstein-Friesian (HF) cattle. Finally, the relationship between PVL levels and BoLA-DRB3 haplotypes was investigated in BLV-infected JBRK cattle.ResultsWe determined the number of BL cases recorded over the past ten years in Kumamoto Prefecture by cattle breed. A limited number of BL cases was observed in JBRK cattle. The proportion of BL cases in the JBRK was lower than that in JB and HF. The PVL was significantly lower in BLV-infected JBRK cattle than that in the JB and HF breeds. Finally, in BLV-infected JBRK cattle, the PVL was not significantly affected by BoLA-DRB3 alleles and haplotypes. BoLA-DRB3 allelic frequency did not differ between BLV-infected JBRK cattle with low PVL and high PVL.ConclusionsTo our knowledge, this is the first report showing that BL occurred less in the JBRK population of Kumamoto Prefecture. After BLV-infection, the PVL was significantly lower in JBRK cattle than that in JB and HF breeds. The genetic factors implicated in maintaining a low PVL have yet to be elucidated, but the BoLA-DRB3 haplotypes are likely not involved.

Relationship between Beef Quality and Bull Breed.

The beef industry in Poland heavily relies on the Polish Holstein-Friesian (PHF) breed, known for its primary use in dairy production, but which also contributes significantly to the beef supply. In contrast, the Limousine (LM), Hereford (HH), and Charolaise (CH) breeds have gained popularity due to their ideal specialized characteristics for beef production. As PHF continues to dominate the beef market, a thorough comparison of its beef quality and nutritional attributes with the three most popular beef breeds in Poland is essential. This study aims to address this knowledge gap by conducting a rigorous comparison. The experiment was carried out on the beef from 67 bulls kept in a free-stall system with standardized feeding. The highest total antioxidant status (TAS) was found in CH and was 147.5% higher than that in PHF. Also, compared with PHF, a large difference of 70% was observed in LM, while in HH it was only 6.25%. For degree of antioxidant potential (DAP), the highest concentration was found in LM, while CH had a slightly lower score than LM. PHF had the lowest scores for each of the analyzed parameters of protein fraction. For anserine, taurine, creatinine, and creatine content, the highest results were found for LM. For carnosine and coenzyme Q10, the highest values were found for CH. Overall, these results highlight the impact of maturity and breed on carcass composition and quality. Late-maturing breeds, such as LM and CH, tend to exhibit leaner carcasses with superior fatty acid profiles and antioxidant properties. This knowledge is valuable for producers, enabling them to make informed decisions regarding breed selection and production strategies to meet specific market demands for beef with the desired composition and quality.

The First Efficient Insemination of Holstein-Friesian Cows, Effect of Age and Body Weight

Age and live weight at the first fruitful insemination of replacement heifers is an important link in the breeding process. A study was made of the influence of several factors on the productive longevity of cows of the Holstein-Friesian breed. The relationship between lifelong milk yield and longevity with the age of the first fruitful insemination is higher (r = 0.10-0.23) than with the live weight at the first insemination (r = 0.04-0.13). The breed of animals does not affect the degree of relationship between age and body weight at the first fruitful insemination and their productive longevity. Increased indicators of productive longevity are characteristic of animals of Gilan Dairy Farms and ASA Animal Husbandry, inseminated at an earlier date of 14-16 months. with increased live weight. Analysis of the results obtained allows us to conclude that the factor ‘farming’ in the dispersion complex had a slight effect on the indicators of productive longevity of cows.