Understanding past long-term vegetation responses to regional or even global climate change and forcing mechanisms is essential to future climate change projections. However, due to the lack of long-term terrestrial sedimentary records, there are few studies focusing on vegetation changes in tropical southern China since the last glacial period, especially from the perspective of peat n-alkane records. Here, we have presented a peat core record from the Xialu peatland in the northern Leizhou Peninsula, and n-alkanes were investigated in conjunction with multiple proxy indicators. Our results showed that the organic matter sources were mainly a mixture of aquatic and terrestrial vegetation, with terrestrial vegetation accounting for most of the bulk organic matter composition. From ∼44.1 to 29 cal kyr BP, the organic matter source was mainly dominated by terrestrial vegetation, which corresponds to warm and humid conditions. From 29 to 14 cal kyr BP, the input of the terrestrial vegetation was reduced, the aquatic vegetation input increased, implying cool and dry conditions. From 14 to 9.3 cal kyr BP, the climate gradually became warmer and wetter, and terrestrial vegetation dominated in this area. Overall, the climatic conditions from the Xialu peatland were generally consistent with other records from adjacent areas. Our results suggest that, during the Last Glacial Maximum (LGM), a substantial drop in regional and global sea levels may have been the main cause of drought in tropical southern China on orbital timescales. Meanwhile, several climatic fluctuations on millennial timescales could have been influenced by the variability of the North Atlantic Meridional Overturning Circulation (AMOC) and migration of the Intertropical Convergence Zone (ITCZ).