Holarctic Species Research Articles

Species delimitation under allopatry: genomic insights within and across continents in Lepidoptera

Abstract Delimitation of allopatric populations into species remains subjective and largely arbitrary. Many cold-adapted species from the sub-Arctic and Central and Southern European Mountain systems provide excellent models to study allopatry problem due to their patchy distributions. The same concerns many Holarctic species, which frequently show varying degrees of differentiation between continents. In this study, we analyze high-throughput target enrichment data for 10 groups of Arctic-alpine and Holarctic lepidopteran species sampled from different regions across the Holarctic realm, i.e., Fennoscandia, European Alps, Altai Mountains, and North America. We first aimed to assess whether the genetic differences in the nuclear genome reflected observed DNA barcode divergences and, secondly, whether the gap between population and species-level differences can be reliably dissected using genomic data. We compared the phylogenetic trees and uncorrected pairwise genetic distances obtained from target enrichment and mitochondrial COI barcodes and performed a suite of population genetic and species delimitation analyses to further explore patterns of intraspecific variation in our study species. We observed that in about one-half of the cases, DNA barcodes showed phylogenetic relationships similar to the target enrichment markers. Nuclear genetic differentiation varied among the populations analyzed, from low differentiation of geographically separated populations to the deeper separation of some Nearctic populations and Arctic-alpine disjunction in the populations from Fennoscandia and Southern European mountains. Our results highlight the need for consistent delimitation of allopatric populations, especially given the prevalence of distributional discontinuities across species. Large sets of standard genetic markers provide a very promising avenue towards this goal.

Development and age estimation of the intrapuparial period of Phormia regina (Diptera: Calliphoridae) for postmortem interval estimation.

Phormia regina (Meigen, 1826; Diptera: Calliphoridae) is a Holarctic species that rapidly colonizes carcasses and has been used as an indicator for determining the minimum postmortem interval. However, studies using morphological methods to estimate the intrapuparial age of P. regina are lacking. In this study, morphological changes within the puparium were observed under a stereomicroscope at 7 constant temperatures ranging from 16 °C to 34 °C. The intrapuparial period was categorized into 12 substages. Morphological indicators, including compound eyes, mouthparts, antennae, thorax, legs, wings, and abdomen, were recorded in detail. The observed morphological changes were divided into 6-10 substages, and the duration of each substage was also recorded in detail. The results of this study provide primary data for using the intrapuparial morphology of P. regina when pupae are collected at a crime scene and estimating the minimum postmortem interval.

Latitudinal distribution of freshwater sponge species across European Russia

This study provides original faunistic data on freshwater sponge dispersal across a huge territory of European Russia from north to south, highlighting the northern limits of thermophilic and north-adapted species. The holarctic species Ephydatia muelleri and, especially, Spongilla lacustris were predominant in northern areas, while the cosmopolitan Ephydatia fluviatilis and Eunapius fragilis were less prevalent. Moving to the south, E. fluviatilis took the lead. The distributional pattern of northern sponges aligns with literature data from North Europe, confirming the prevalence of the holarctic species in high latitudes. Tubella (=Trochospongilla) horrida, Eunapius carteri, and Radiospongilla cerebellata are considered to be thermophilic species that could not tolerate the conditions in northern regions. Notable findings include the extension of Eunapius carteri's known range and the first report of R. cerebellata within Russia, which is also the second record for Europe. Three potentially expected species were not found in the studied area, indicating regional rarity. We also provide molecular and morphological evidence for the synonymy of Spongilla arctica and Spongilla lacustris. Finally, we discuss the impacts of climate change and agricultural activities on northern and southern sponge habitats respectively.

Biogeography and ecology of the Algerian island flora

Background and aims – In spite of their proximity to the coasts, the flora of the islands and islets of the Algerian coasts has been poorly studied. This work is the first to provide an overall view of the richness and the determinants of the Algerian island flora. Material and methods – The study is based on a compilation of original floristic data. Two classifications of 30 islands and islets were derived from PCA and HCPC performed on the species occurrence matrix and on a matrix of flora descriptors including species richness, functional traits (life form, seed dispersal, pollination), and biogeographic range. We performed an RDA to explain the variation in flora characteristics by a set of physiographic (longitude, latitude, elevation, area, isolation, steepness index, and area/perimeter ratio) and biotic (seabird density and human presence) variables. Key results – The floristic inventory encompassed a total richness of 295 vascular plant taxa (including subspecies and varieties) on the 30 studied sites. Five main groups of islands and islets can be distinguished based on vegetation composition and three from flora descriptors. RDA model selection revealed that the combination of four variables (seabird density, area, latitude, and longitude) explained 26.6% of the variation in flora characteristics. Taken alone, the density of yellow-legged gull colonies and the island area were the main drivers of this variation. Results showed that floristic richness was associated with larger island area and higher seabird density, the importance of some plant functional traits, such as zoochory and entomogamy, and with a higher proportion of Eurasian holarctic species. Conclusion – In the context of island flora conservation, some of these small islands of Algeria can be considered as “modern refuges” from human pressures, and this is particularly important in the context of Mediterranean ecosystems characterised by a quasi-permanent human impact in various habitats. Two new important plant areas (IPAs) for Algeria are proposed following the results of these floristic inventories.

Catalogue of Asplenium septentrionale (L.) (Tracheophyta, Polypodiopsida, Aspleniaseae) specimens deposited in the State Museum of Natural History NASU, Lviv, Ukraine

The detailed analysis of origin of the Asplenium septentrionale (L.) Hoffm. specimens, deposited in the main collection of vascular plants’ herbarium in the SMNH NASU, has been carried out. Asplenium septentrionale (L.) Hoffm. – northern spleenwort – is a perennial fern of the genus Asplenium, the family Aspleniaceae, a Holarctic petrophilous species with a disjunctive Eurasian-North American natural range (Europe, the Caucasus, Central Asia, Western Siberia, Mongolia, Himalayas, Far East, North America). Its localities are confined to petrophilic communities, mainly on granite outcrops devoid of calcium (rock crevices, rocky slopes, landslides, boulders, shady spruce forests, etc.), and the species rises to the upper mountain belt. In Ukraine, this species occurs within the Carpathians, the Crimea, Steppe (Prydniprovska and Azov uplands, Donetsk Ridge) and Forest-Steppe (Volynska and Prydniprovska uplands) zones, Polissya (occasionally in Volynske and Zhytomyrske Polissya). Asplenium septentrionale (L.) Hoffm. has been included into the official lists of regionally rare plants in many regions of Ukraine (Dnipropetrovska, Donetska, Zhytomyrska, Zaporizka, Ivano-Frankivska, Kyivska, Kirovohradska, Luhanska, Rivnenska, Chernivetska provinces and the Autonomous Republic of the Crimea). The main collection of the Herbarium of Vascular Plants in the SMNH NASU contains 26 herbarium specimens of A. septentrionale (L.) Hoffm., all being included into the electronic database "Biodiversity of Ukraine" (http://dc.smnh.org/) and digitized. The geography of the herbarium collections of A. septentrionale (L.) Hoffm., besides the modern territory of Ukraine (Dnipropetrovska (1901), Donetska (1939, 1947, 1965, 1966), Zhytomyrska (1949), Zakarpatska (1948, 1957, 1961, 1969), Ivano-Frankivska (1967), Kirovohradska (1900), Rivnenska (1948) provinces and the Autonomous Republic of the Crimea (1900)), has been represented also by the territories of Poland (1905), Czech Republic, Slovakia, Austria, Romania (1905), Finland (1898) and the Russian Federation (North Ossetia, 1898). In descending order of the number of collected herbarium specimens with the participation of 16 collectors, the following series has been formed: Slobodian M. (3 herbarium specimens), Vainagii I. (2), Voloshchak E. (2), Hanieshyn S. (2), Lazebna A. (2), Malynovskyi K. (2), Zinger M. (2), Berko J. (1), Golde K. (1), Kotov M, Karnaukh E. (1), Markovych V. (1), Melnychuk V. (1), Rheman A. (1), Stoyko S. (1), Finn V. (1), Khrzhyzhanovskyi V., Lazebna A. (1), Shokhin V. (1). The chronology of the herbarium collections covers the period from 1898 to 1969. The earliest specimens (second half of the 19th century) were collected in Finland (V. Shokhin collection) and North Ossetia (V. Markovych collection). This article was written with the support of the National Research Foundation of Ukraine, call 2022.01 «Science for the Recovery of Ukraine in the War and Post-War Periods» the project 2022.01/0013 «Digitization of natural history collections damaged as a result of hostilities and related factors: development of protocols and implementation on the basis of the State Museum of Natural History of the National Academy of Sciences of Ukraine». The contents of this article do not necessarily reflect the views of the National Research Foundation of Ukraine and are the sole responsibility of the State Museum of Natural History, NAS of Ukraine.

Free-living mites (Acari) of the Shokalsky Island, off the northern Gyda Peninsula, Kara Sea, High Arctic

The fauna of free-living mites of the Shokalsky Island, off the Gyda Peninsula, Kara Sea, totals at least 81 species belonging to 36 families. The suborder Prostigmata includes 38 species, vs 18 of Oribatida, 17 of Mesostigmata, 5 of Endeostigmata, and 3 of Astigmata. The most species-rich families are Ascidae (9 species), Eupodidae (7), Tarsonemidae (4), and Ceratozetidae (4). However, most genera (77%) are represented by one species only. Among mites identified to the species level, the Holarctic species prevail which show mainly arctic, arctic-montane, and arctic-boreal distribution patterns. The abundance of soil mites varies with 10160–299440 ind./m<sup>2</sup>, being the maximum in the soil of both dryad tundra and in a polar fox den on a hillock. Prostigmata are the most diverse mite group throughout, but the dominant species include either prostigmatic or endeostigmatic, or oribatid mites. The rare species, Charadracarus hurdi Newell, 1960, hitherto known only from Point Barrow, Alaska, has been found for the first time since its original description. Some features of the acarofauna of the Shokalsky Island are transitional between those characteristics of the polar desert and tundra zones.

Zoogeographical composition of macroinvertebrates in low-mountain lakes of the Russian Altai

In 2022 the taxonomic and zoogeographic composition of the macrozoobenthos of six lowland lakes of the Russian Altai: Aya, Beloe, Kireevo, Kolyvanskoe, Koksha and Svetloye was investigated. In the studied lakes 156 species of zoobenthos from 9 classes of invertebrates were identified. The largest number of species is characteristic of the insect class. Chironomids and oligochaetes dominated in terms of frequency of occurrence in the lakes. Of the oligochaetes, the family Tubificidae (68%) was more frequently observed. Tubificidae (68%), chironomids — subfamilies Chironominae (91%) and Tanypodinae (66%). The maximum species diversity of zoobenthos was observed in lakes Beloe (81 species) and Koksha (76 species), the least number of species was found in lakes Aya (41 species) and Kireevo (52 species). Lakes Beloe — Kireevo — Kolyvanskoe and Koksha — Svetloye are the most similar in composition of benthic invertebrates. The most original was the taxonomic composition of the deepest lake Aya. According to the taxonomic composition of the fauna of bottom invertebrates, most lakes (Aya, Beloe, Kireevo, Kolyvanskoe) were closer to plain water bodies, two lakes (Koksha, Svetloe) combined features of both plain and mountain lakes. Zoogeographic analysis of the fauna of benthic invertebrates of foothill lakes revealed 12 types of distribution. Holarctic (39%), Palaearctic (23%) and Western Palaearctic species (21%) were predominant in the lake fauna. Comparative analysis of the fauna of the identified homotopic and heterotopic species showed that the most extensive areas are found only among homotopic primary aquatic archaic taxa. Zoogeographic analysis of the studied lakes indicate their proximity to the border of the Western and Eastern Palearctic.

On Ypsolopha micromoths (Lepidoptera, Ypsolophidae) associated with Adesmia shrubs (Fabaceae) in the arid western slope of the central Andes.

Ypsolopha Latreille, 1796 (Lepidoptera, Ypsolophidae) is a genus comprised mostly of Holarctic micromoth species with a fairly broad range of larval hosts (e.g. Aceraceae, Rosaceae, and Fagaceae). The only previous record of herbivory on a representative of the South American genus Adesmia DC. (Fabaceae) was based on the discovery of Ypsolophamoltenii Vargas, 2018 larvae feeding on Adesmiaverrucosa Meyen in the Andes of northern Chile. Further surveys revealed Adesmiaatacamensis Phil. as another host for Y.moltenii, and Adesmiaspinosissima Meyen as the single host of Ypsolopha sp. The genetic distance between DNA barcodes of the two micromoth species was 7.9-8.1% (K2P). These results suggest narrow host ranges for Adesmia-feeding Ypsolopha and highlight the need to further explore the taxonomic diversity of these micromoths in other South American environments.

Wing lengths of three Arctic butterfly species decrease as summers warm in Alaska

Climate warming can cause arthropods to express plastic and/or evolved changes in morphology. Previous studies have demonstrated that body sizes of Arctic butterflies are influenced by the temperatures experienced as larvae. To investigate whether this was occurring among Alaskan butterflies, we analyzed temporal trends in the wing sizes of three Holarctic species, Colias hecla, Boloria chariclea and Boloria freija, using museum specimens collected in Arctic tundra regions of Alaska between 1971 and 1995. Wing length was compared to accumulated growing degree days (GDD) during both the spring of the year collected and the previous year's summer during the normal period of larval development. We used mixed‐effects models to test if spring and summer temperatures affected adult morphology. Results show that for every 1°C increase in average seasonal temperature, wingspans decreased between 0.7 and 5 mm, with B. freija the most strongly affected. Our results suggest that the morphological sensitivity of Arctic butterflies to warming is the outcome of interactions between life‐history traits and regional climate, with all species sensitive to warming the summer before the flight year as well as warming the spring of the flight year. Boloria freija, which overwinters as late instar larvae that do not feed before pupation the following spring, was particularly strongly affected by summer warming.

Ants of Kamchatka: checklist, DNA-barcoding and key (Hymenoptera, Formicidae)

Kamchatka is a remote volcanic peninsula in the sub-arctic Far East of Russia. Its myrmecofauna has been scientifically addressed several times. However, previous species lists are contradictory in part and a modern study seems to be lacking. Based on literature review, I conclude that 12 species and 4 genera (Camponotus, Formica, Leptothorax and Myrmica) of ants may be native to Kamchatka. Of those, 9 were found in the field, 3 having a Holarctic, 3 a trans-Palearctic and 3 an East Palearctic distribution. Most species diversity was found at mid-elevations, in pine shrubs and mixed forests, while deciduous forests and open habitats at lower altitudes were surprisingly species poor. DNA-barcoding was performed for 57 specimens/9 species. Genetic diversity (COI) was low for 8/9 species, with the exception of Leptothorax acervorum, for which all samples were of a different haplotype and 2 haplogroups were identified. For the encountered Holarctic and trans-Palearctic species, closely related haplotypes (<0.7% raw distance) occur in Europe and/or North America. Some Formica ants were morphologically atypical, with workers partially resembling those of F. fusca, while gynes identified as F. lemani. Morphometric analysis and DNA-barcoding suggested that all these specimens belong to a single species, F. lemani. Standard images for all specimens and an illustrated key to the worker caste are provided.

New locality Goodyera repens (L.) R. BR. (Orchidaceae) on the southern border of the range (Male Polissya)

Goodyera repens (Orchidaceae) is a Holarctic species, evolutionarily and ecologically related to the forest formations of the dark coniferous taiga. In Ukraine, it occurs in the northern flat part of the forest-steppe zone, mainly in the Poliska lowland, in the Carpathians and the Crimean mountains.

Molecular evidence for multiple origins and high genetic differentiation of non-native winter crane fly, Trichocera maculipennis (Diptera: Trichoceridae), in the maritime Antarctic

Native biodiversity and ecosystems of Antarctica safeguarded from biological invasion face recent threats from non-native species, accelerated by increasing human activities and climate changes. Over two decades ago, the winter crane fly, Trichocera maculipennis, was first detected on King George Island. It has now successfully colonized several research stations across King George Island. To understand the origin, genetic diversity, and population structure of this Holarctic species, we conducted mitochondrial DNA cytochrome c oxidase subunit I (COI) sequence analysis across both its native and invasive ranges. In parallel, we performed microsatellite loci analysis within the invasive ranges, utilizing 12 polymorphic microsatellite markers. Furthermore, we compared body sizes among adult males and females collected from three different locations of King George Island. Our COI sequence analysis exhibited two different lineages present on King George Island. Lineage I was linked to Arctic Svalbard and Polish cave populations and Lineage II was related to Canadian Terra Nova National Park populations, implying multiple origins. Microsatellite analysis further exhibited high levels of genetic diversity and significant levels of genetic differentiation among invasive populations. Body sizes of adult T. maculipennis were significantly different among invasive populations but were not attributed to genetics. This significant genetic diversity likely facilitated the rapid colonization and establishment of T. maculipennis on King George Island, contributing to their successful invasion. Molecular analysis results revealed a substantial amount of genetic variation within invasive populations, which can serve as management units for invasive species control. Furthermore, the genetic markers we developed in the study will be invaluable tools for tracking impending invasion events and the travel routes of new individuals. Taken together, these findings illustrate the highly invasive and adaptable characteristics of T. maculipennis. Therefore, immediate action is necessary to mitigate their ongoing invasion and facilitate their eradication.

Comparison of Olfactory Sensilla Structure in Littoral and Deep-Water Amphipods from the Baikal Region

The structure of olfactory sensilla was compared in amphipods from the Baikal region, namely in nine Baikal species (Ommatogammarus carneolus melanophthalmus Bazikalova, 1945, O. flavus (Dybowsky, 1874), O. albinus (Dybowsky, 1874), Eulimnogammarus verrucosus (Gerstfeldt, 1858), E. vittatus (Dybowsky, 1874), E. cyaneus (Dybowsky, 1874), Acanthogammarus godlewskii (Dybowsky, 1874), Pallasea cancelloides (Gerstfeldt, 1858) and Gmelinoides fasciatus (Stebbing, 1899)) and a Holarctic species Gammarus lacustris Sars, 1863. The results of the study demonstrate the comparison of aesthetasc lengths and numbers, as well as SEM images of the aesthetascs for Baikal amphipods for the first time. Our results confirm and complement the previously obtained data on the number of aesthetascs per segment for three deep-water Baikal amphipod species of the genus Ommatogammarus. Within the studied deep-water amphipods with different habitat depth ranges, Ommatogammarus albinus (Dybowsky, 1874), inhabiting maximum habitat depth, had the highest number of aesthetascs. The size and number of aesthetascs did not differ in males and females of the littoral Eulimnogammarus verrucosus (Gerstfeldt, 1858) and deep-water species. The littoral amphipods were shown to have equipped with more mechanoreceptors antennae I than deep-water amphipods.

Holarctic Species in the Pluteus podospileus Clade: Description of Six New Species and Reassessment of Old Names.

We studied the taxonomy of Pluteus podospileus and similar species using morphological and molecular (nrITS, TEF1-α) data, including a detailed study of the type collections of P. inflatus var. alneus, Pluteus minutissimus f. major, and P. granulatus var. tenellus. Within the P. podospileus complex, we phylogenetically confirmed six species in Europe, five in Asia, and eight in North America. Based on our results, we recognize P. seticeps as a separate species occurring in North America, while P. podospileus is limited to Eurasia. We describe six new species and a new variety: P. absconditus, P. fuscodiscus, P. gausapatus, P. inexpectatus, P. millsii, and P. notabilis and its variety, P. notabilis var. insignis. We elevate Pluteus seticeps var. cystidiosus to species rank as Pluteus cystidiosus. Based on the holotype of P. inflatus var. alneus, collections of P. inflatus identified by Velenovský, and several modern collections, we resurrect the name P. inflatus. Based on molecular analyses of syntypes of Pluteus minutissimus f. major and a holotype of Pluteus granulatus var. tenellus, we synonymize them under P. inflatus. We also increase our knowledge about the morphology and distribution of P. cutefractus.

Rhizosphere effects on the microbial community: Specificity and conservatism across geographically disjunct Panax species

A deeper understanding of plant control over the rhizosphere, especially from the evolutionary perspective, is needed for environmentally friendly breeding to take advantage of soil microbes. In the present study, paired bulk and rhizosphere soil samples from 810 plants of three Panax species (P. ginseng, PG; P. quinquefolium, PQ; P. notoginseng, PN) were used to assess rhizosphere effects on microbial diversity, phylogenetic structure, and community composition. The rhizosphere effects were generally stronger in tetraploid PG/PQ than in diploid PN, and the edaphic factors capable of explaining within-species effect variations differed among the three species. However, these species were consistent in terms of the greater biodiversity and stronger phylogenetic clustering in the rhizosphere than in bulk soil, as well as the upward trend of effect strength with plant age. The rhizosphere-enriched taxa of the three species showed strong phylogenetic clustering, as indicated by phylogenetic sampling theory (z score = −11.33) and UniFrac distances, and the coenrichment was mainly reflected in specific orders of Alpha- and Gammaproteobacteria. The potential ecological similarity of rhizosphere-enriched taxa might represent the evolutionarily conserved part of genetic traits involved in rhizosphere recruitment and microbial function requirements in the three species. In summary, this study reveals the specificity and conservatism of rhizosphere effect evolution in these related but Holarctic disjunct Panax species, which advances our understanding of plant–microbe coevolution in the rhizosphere.

Clarifying the taxonomic status and exploring hidden diversity in Graphomya minor Robineau-Desvoidy, 1830 (Diptera: Muscidae: Mydaeinae) using molecular and morphological evidence

Summary A first report on the phylogeography and phylogeny of the widespread Holarctic species, Graphomya minor Robineau-Desvoidy, 1830, within its continuous range throughout northern Eurasia and North America, is presented. The geographic patterns of genetic and morphological variability in G. minor were examined. The phylogenetic structure was assessed using the barcode fragment of the mitochondrial COI gene. We detected 35 unique COI haplotypes in in G. minor, with only two common haplotypes for both the Palaearctic and Nearctic regions. A higher number of COI haplotypes was detected in the Eurasian populations (27 haplotypes) than in the North American samples (eight haplotypes). The diversity of mitochondrial DNA haplotypes and the degree of genetic differences between five geographic populations of G. minor varies. Morphological analysis did not reveal correlations between haplotypes and morphological characters. A genetic comparison of G. minor with morphologically close Palaearctic (G. maculata, G. rufitibia) and Nearctic species (G. ungava, G. minuta, G. americana, G. transitionis) was made. The newly obtained COI sequences of Graphomya species (155) were combined with those published in the GenBank and BOLD databases (76) and were analysed using the species delimitation software ASAP. The results of these species delimitation analyses validated the morphological species in Graphomya and revealed two new species lineages of Graphomya located in north-eastern and south-eastern Russia. Based on the results of our analyses, we concluded that G. minor is a valid widespread polymorphic Holarctic species and is not a junior synonym of G. maculata; and assign 30 unidentified sequences from BOLD and GenBank to named morphospecies.

Exploring the diversity of the Malagasy Ponera (Hymenoptera: Formicidae) fauna via integrative taxonomy

The genus Ponera includes over 60 extant species worldwide. These tiny, endogeic predator ants are predominantly distributed in the Indomalaya and Australasia regions, with a few additional Holarctic species. Herein, we explore and describe the diversity of the Malagasy Ponera fauna through an integrative taxonomic approach. We obtained our morphological species hypotheses from multivariate analyses of ten continuous morphometric characters. Species boundaries and reliability of morphological clusters were tested via confirmatory Linear Discriminant Analysis (LDA), cross-validation (LOOCV), and analyses of a mitochondrial COI gene fragment. According to the combined application of the analyses, altogether, three species are inferred in the Malagasy region, Ponera petila Wilson (1957), P. swezeyi Wheeler (1933), and P. adumbrans Csősz & Fisher sp. n. Ponera petila and P. swezeyi belong to the Indo-Australian Ponera tenuis group; the third species, P. adumbrans sp. n., is morphologically similar to the Papua New Guinean P. clavicornis Emery (1900). Furthermore, Linear Discriminant Analysis classified the type specimens of P. bableti Perrault (1993), along with a P. petila cluster with posterior p = 1. Therefore, we propose the new junior synonymy of P. bableti with P. petila. Madagascar’s extant biodiversity is predominantly explained by colonization events from the African continent across the Mozambique channel via rafting. However, since no native Ponera species are known from the Afrotropical continent, and the closest congeners have an almost exclusively Indo-Australian distribution, the likelihood of an Indo-Australian origin of the Malagasy Ponera fauna is implied.

Faunistic inventory, identification keys and zoogeographical analysis of the Thysanoptera-Terebrantia of Saudi Arabia, including two new species.

This faunistic study of the Thysanoptera suborder Terebrantia of Saudi Arabia has revealed 79 species in 39 genera and four families. Two families Melanthripidae Bagnall and Stenurothripidae Bagnall, also 25 genera and 51 species, are newly recorded for Saudi Arabia, and Mycterothrips arabicus sp. n. and Scirtothrips erectus sp. n., are described. Males of Thrips lomatus zur Strassan and Odontothrips elbaensis Priesner are described for the first time. Keys to the families, genera and species of Terebrantia of Saudi Arabia are presented, together with a species check list. Zoogeographical analysis indicates that general affinity of the thrips fauna of Saudi Arabia is dominated by Afrotropical (22.6%) and Saharo-Arabian (17.9%) species, followed by Cosmopolitan (16.7%), endemic (11.9%), Palearctic (10.7%), Oriental (9.5%), Mediterranean (7.1%), and traces of Nearctic (2.4%) and Holarctic (1.2%) species.

Oribatid Mites (Oribatida) Associated with Nests of Hollow-Nesting Birds, on the Example of a Model Species, the European Pied Flycatcher (Ficedula hypoleuca), in the Taiga Forests of the European North-East of Russia

The authors have obtained original material on the fauna and population structure of oribatid mites inhabiting nests of the European Pied Flycatcher (Ficedula hypoleuca, Passeriformes, hollow-nesting bird) on the territory of the taiga zone of the European North-East of Russia. Long-term research and the collection of nests were carried out in the green zone of Syktyvkar in 2017–2022. Observations were made for artificial nests (hollows) of a box type with a bottom area of 100 cm2. The material of the tray was collected completely. In 135 studied nests of Pied Flycatchers, 1762 specimens were found and identified for 22 species of oribatid mites from 19 genera and 16 families. In the nests of the Pied Flycatcher, a complex of species was found that is known as an arboricolous species for this region; these are Oribatula (Zygoribatula) propinqua, Oribatula (Z.) exilis, Trichoribates (T.) berlesei, and Ameronothrus oblongus. We suggested that arboricolous species, as well as eurytopic species, can actively inhabit bird nests. Highly numerous in our collections were representatives of the Oribatulidae and Scheloribatidae families; they are Oribatula (Z.) propinqua, Oribatula (Z.) exilis, Oribatula (O.) tibialis, and Scheloribates laevigatus. Epigeic species are dominated by the species number. The fauna of oribatid mites mainly included widespread Holarctic species (54.54%).

The record of Ametropus fragilis Albarda, 1878 (Ephemeroptera, Ametropodidae) from Georgia

The representatives of the genus Ametropus (Insecta, Ephemeroptera) were recorded 80 years ago in Georgia. Due to the taxonomic uncertainty, the recorded specimens were considered potentially new taxa. Since then, no other record of Ametropus has been documented from the whole South Caucasus and its nearby regions further south. In the present contribution, we provide a new record of Ametropus from the Alazani River (Kura River Basin, Georgia). Morphological and DNA barcoding of the COI gene fragment showed that the species belongs to the widespread Holarctic species A. fragilis. Morpho-anatomical characteristics also confirmed the high degree of similarity between the newly recorded specimens and those once recorded 80 years ago. In addition, the COI barcode showed a strikingly closer relationship between Caucasian A. fragilis and North American A. neavei (3.54% divergence) compared to Caucasian and European A. fragilis populations (4.3% divergence). The observed pattern clearly indicates the need for further and more thorough revision of the Ametropus species complex in the Holarctic region.