Abstract BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (COVID-19) is one of the worst health-care problem humankind has faced in the last few centuries. Understanding the population’s antibody responses is crucial for effective decision-making while implementing public health interventions in view of the COVID-19 pandemic. The study was done to know the immune status of healthy donors to COVID-19 infections and to know the duration of persistence of immunoglobulin G (IgG) antibodies with respect to infections and vaccination. AIMS: This study aimed to determine the prevalence of SARS-CoV-2 IgG antibodies in healthy blood donors in our tertiary care center. MATERIALS AND METHODS: This was a cross-sectional analytical study conducted for a period of 1 year in our blood center. Our study had a sample size of 809 healthy blood donors. Samples of the first 81 donors of each month were chosen. Donor details such as previous COVID positivity, symptoms, hospitalization, comorbidities, contact history, and vaccination status were collected using a structured questionnaire. A 5-ml sample was collected and was tested using Qualisa COVID-19 IgG enzyme-linked immunosorbent assay detection kits. The antigens used for coating the kits were recombinant antigens representing SARS-CoV-2 S1 protein. Data were analyzed using SPSS version 21. The prevalence of positive IgG seroprevalence of COVID-19 antibodies was reported using proportion along with 95% confidence interval. Association between all the categorical variables and the presence of seropositive status for IgG antibodies was assessed using the Chi-square test. P <0.05 was considered statistically significant. RESULTS: The seroprevalence of COVID-19 antibodies in our study participants was 82.3%. There is a significant association between COVID-19 infectivity, vaccination, with seroprevalence. There was a seropositivity of 51% among those who were neither infected nor vaccinated, which may be due to the development of herd immunity in our population. CONCLUSIONS: Population who were neither vaccinated nor had a history of infection also had a significant prevalence of antibodies suggesting the development of herd immunity in our community. The present study will add to the knowledge and better understanding of COVID-19 antibodies in the existing literature. Further studies will be required to verify our findings and to know the persistent nature of COVID antibodies.