This study systematically investigates the catalytic effect of histidine (HIS) on CO2 desorption in amine-based solvents, with a primary focus on 30 wt% N-(2-aminoethylamino)ethanol (AEEA) and its blends with N-methyldiethanolamine (MDEA). Experimental results show that the addition of 0.22 wt% HIS significantly enhances both the equilibrium desorption amount and the maximum desorption rate of CO2, particularly at elevated temperatures (e.g., 100 °C). Under optimal conditions, HIS increased the maximum desorption rate by 22.1% and reduced the heat duty to 71.7% compared to the non-catalytic benchmark. The catalytic performance was further confirmed in AEEA-MDEA mixed solvents, with the most pronounced effect observed in the 3:2 molar ratio system, where HIS enhanced both the equilibrium desorption amount and the maximum desorption rate by 15.3% and 20.8%, respectively. Through 13C NMR analysis and pH-dependent speciation monitoring, we revealed that HIS alters the reaction pathway by suppressing the formation of stable carbamate species (AEEA(a)COO−). The protonated (HIS+) and neutral (HIS±) forms were identified as the active species that promote more direct CO2 release from carbamate, while the deprotonated (HIS−) form facilitates proton transfer and amine regeneration. HIS also exhibited excellent catalytic stability over 10 absorption–desorption cycles. These findings highlight HIS as an efficient and stable organocatalyst for energy-efficient CO2 desorption processes.

Hepatocellular carcinoma (HepG2) is a highly aggressive liver cancer with poor prognosis, limited treatment options, and high mortality rates, making it a serious global health concern that demands urgent development of more effective and safer therapeutic approaches. In this context, the present study focuses on the green synthesis of SrO2 nanoparticles using Clitoria ternatea flower extract, followed by surface modification with Pluronic F127 (PF127) and L-histidine (LH), to fabricate SrO2-PF127-LH nanocomposites aimed at evaluating their potential anticancer efficacy against the HepG2 cell line. Various analytical techniques were used to characterize the nanocomposite, and then their anticancer activity against HePG2 liver cancer cells, antioxidant properties, and antimicrobial activity against the bacteria mentioned above were evaluated. XRD revealed the crystalline nature of SrO2 with atetragonal phase. FTIR spectrum confirmed the Sr-O stretching band at 573cm-1 for SrO2-PF127-LH nanocomposite. UV-visible analysis revealed the band gap energies of 4.13eV for SrO2 and 4.07eV forSrO2-PF127-LH nanocomposite. The surface defects including oxygen vacancies of SrO2-PF127-LH nanocomposite were investigated using PL analysis. The SrO2-PF127-LH nanocomposite exhibited excellent antibacterial and antioxidant activities when compared to SrO2 nanoparticles alone. In addition, the SrO2-PF127-LH nanocomposite had enhanced anticancer activity against liver cancer (HePG2) cell lines.

We present a two-step method for the direct estimation of the energy of individual non-covalent interactions (NCIs) such as hydrogen bond (HB), CH…π or π…π interactions in any given molecular crystal. The first step of this method is to calculate the energy of a NCI by a molecular tailoring approach based (MTA-based) method utilizing a sufficiently large molecular crystal structure. This calculation is performed at the low Hartree-Fock (HF) level. In the next step, the energy of the same referenced NCI is evaluated by the MTA-based method employing a suitable monomeric or dimeric species. This calculation is usually performed at both the high (B3LYP, MP2 or CCSD(T)) and low (HF) levels. Note that the NCI energies in monomeric or dimeric species are significantly different from those in the actual crystal. The difference in the energy calculated in the second step at high and low levels is added to the energy of this NCI calculated (at HF level) in the first step employing a large molecular crystal. The energies of NCIs calculated by this two-step method are compared with their actual crystal counterparts. For this purpose, molecular crystals of L-Histidine (LH), nitromalonamide (NMA) and salicylic acid (SA) are chosen as test cases. It is found that the proposed two-step method provides very accurate energy of individual NCIs in these molecular crystals. For instance, the estimated energies of NCIs by the proposed two-step method are in excellent linear agreement with their actual crystal counterparts (R2 = 0.9983). Furthermore, RMSD and standard deviation are 0.22 and 0.24 kcal/mol, respectively, with a mean and maximum absolute error being 0.15 and 0.51 kcal/mol, respectively. Importantly, the two-step method is computationally efficient and saves nearly 50% of computational time vis-à-vis its full calculation counterpart employing the actual molecular crystal.

Histidine (HIS) is an essential amino acid (AA) with key physiological roles in metal chelation and proton buffering. Its three nitrogen (N) atoms─one α-amino and two in the imidazole side chain─are incorporated through distinct biosynthetic pathways and undergo different catabolic processes. Thus, its intramolecular δ15N values likely provide additional information on these pathways and associated N fluxes. Very few studies have reported molecular average δ15NHIS (δ15NHIS-Total) values, and there are no reported intramolecular δ15NHIS data for natural materials due to technical limitations of available methods. Here, we present a novel analytical approach for compound-specific and intramolecular δ15N values of poly-nitrogenous AAs using HIS as an example. This scheme can be adapted to obtain position-specific δ15N values of other poly-nitrogenous AAs such as glutamine. Underivatized HIS is separated by ion-exchange chromatography (IC) and divided into two aliquots. One fraction is fully oxidized to NO3- using UV-persulfate oxidation for δ15NHIS-Total measurement, while the other undergoes NaClO oxidation, selectively converting α-N and a minor fraction of side chain-N to NO2- at a known ratio. The δ15NHIS values of α-N (δ15NHIS-α) and side chain-N (δ15NHIS-s) are then calculated from these two results. Our findings reveal that α-N is consistently enriched in 15N relative to side chain-N in both commercial HIS powder (Δδ15Nα-s = ∼ +8‰) and biological samples (Δδ15Nα-s = ∼+3 to 25‰), likely due to preferential α-N catabolism via deamination. This finding supports the potential of studying diverse biosynthetic and catabolic processes of poly-nitrogenous AAs using intramolecular N isotope analysis.

Multi-tissue metabolomics and network pharmacology study on the intervention of Danggui Buxue Decoction in mice with gemcitabine induced myelosuppression.

Doxorubicin (DOX), as a potent anti-cancer agent, exerts side effects in vital organs. Various chemical compounds with tissue protective properties are used to prevent the side effects of DOX. This study was planned to investigate the effects of histidine (HIS) and N-acetylcysteine (NAC) ​​on DOX-induced acute kidney injury. The possible mechanisms were followed by determining the histopathological changes of the kidney along with the biochemical alterations of the blood and kidney tissue. Forty-eight rats were divided into eight groups of six animals each to receive normal saline and DOX after alone and combined treatments with HIS and NAC. The DOX at a single dose of 15.00 mg kg-1 was intraperitoneally injected on day one. The separate and combined intraperitoneally injections of HIS and NAC at a similar dose of 100 mg kg-1 were began 30 min after DOX administration and continued for seven consecutive days. The DOX increased kidney weight and caused congestion, hemorrhages and degeneration in kidney tissue. It also increased serum urea and creatinine concentrations and kidney tissue levels of malondialdehyde, tumor necrosis factor-alpha and caspase-3, and decreased superoxide dismutase activity in this tissue. Separate and combined treatments with HIS and NAC improved all the above-mentioned effects of DOX. The restoring effects of the combined treatment were more prominent than the effect of amino acids alone. It was concluded that anti-oxidative, anti-inflammatory and anti-apoptotic mechanisms might be related to the tissue protective effects of HIS and NAC against DOX-induced acute renal injury.

Amino acid parameters provide important and relevant data for objective assessment of physiological and biochemical status of organism and patterns of formation of economically useful traits of animals. The objective of this work is to model phenotypic and genetic patterns of variability of main amino acid parameters of blood of pig hybrids within the framework of mixed equation model. Main 19 amino acid parameters of blood serum of 58 heads of three-breed hybrids (Large White × Landrace × Duroc) were obtained and studied: content (g/100 ml) of aspartic acid and its amide (ASP+ ASN) was 0.64; glutamic acid and its amide (GLU+GLN) ‒ 1.04; alanine (ALA) – 0.46; glycine (GLY) – 0.28; threonine (THR) – 0.34, serine (SER) – 0.39; cysteine (CYS) – 0.21; methionine (MET) –0.07; valine (VAL) – 0.52; isoleucine (ILE) – 0.28; leucine (LEU) –0.83; tyrosine (TYR) – 0.46; phenylalanine (PHE – 0.50); histidine (HIS) – 0.31; lysine (LYS) – 0.69; arginine (ARG) – 0.51; proline (PRO) – 0.41. To calculate the correlation values, covariance and variance of features, models using the REMLF90 programs were fulfilled. It was shown that all amino acids have moderate and strong both phenotypic and genetic correlations (r> 0.5). All amino acids have a fairly close connection with the main biochemical indices. From the above, we can conclude that the equation of the mixed model allows us to obtain a deep understanding of the nature of the variability of the main amino acid indices of the blood, connected in a complex labile biochemical system.

Nutraceuticals can affect transactions immunologically for health and endocrinologically for production and reproduction, specifically, referring to bioactive peptidomemics and saccharomemics in disease resistance and immunlogical balance. The nutraceuticals: Vit D2/D3, fructans and WSCs, PUFAs, alpha-lactoferrin and polysaccharides can affect SCI and as indicated by blood biomarkers. The functional amino acids (FAAs): histidine (HIS), arginine (ARG), lysine (LYS) and leucine (LEU) can affect lean body mass (LBM) accretion and milk production with bovine growth hormone (bGH)/bovine growth-releasing hormone (bGRH) and prolactin. The two prebiotic nutraceuticals referred to can be applied to “designer” oligomers from enriched seed proteins and polysaccharides to improve feed nutritive value (NV). High non-fibrous carbohydrate (NFC) and water-soluble carbohydrate (WSC) grasses can also provide higher-end energy forages. There are, thus, proteinogenic approaches that can be used for supplemental feeding. It is suggested that lower quality residual feedstocks can be converted to food-feed applications and can involve pretreating of fibrous carbohydrates (FC) and NFC, conversion to natural sugars and sweeteners, and “shuffling” copolymerization. Applications can be made to fishmeal for production and health. Slowed reaction enzymes (SRE) can be used with so-called osmolytic resins to study behaviour of pre-formed amino acids (PFAAs) in the rumen milieu to fit rumen protein solubility with use of inhibitors to both plant and microbial proteases, modulating as a result the MCP and “escape” protein flows to the lower GI tract. Clean tech can produce seed-derived proteins using “bulk” cell culturing (processed and unprocessed) and extracted/enriched yeast culturing. There is a need to verify the prebiotic binding receptors in the small intestines (SI) (e. g. gut-associated lymphoid tissues, GALT, and as speculated the SI’s jejunum).

Regulation of histidine metabolism by Lactobacillus Reuteri mediates the pathogenesis and treatment of ischemic stroke

Mixed tin-lead perovskite solar cells (PSCs) have garnered much attention for their ideal bandgap and high environmental research value. However, poly (3,4-ethylenedioxythiophene): poly (styrene sulfonate) (PEDOT: PSS), widely used as a hole transport layer (HTL) for Sn-Pb PSCs, results in unsatisfactory power conversion efficiency (PCE) and long-term stability of PSCs due to its acidity and moisture absorption. A synergistic strategy by incorporating histidine (HIS) into the PEDOT: PSS HTL is applied to simultaneously regulate the nucleation and crystallization of perovskite (PVK). HIS neutralizes the acidity of PEDOT: PSS and enhances conductivity. Especially, the coordination of the C═N and -COO- functional groups in the HIS molecule with Sn2+ and Pb2+ induces vertical growth of PVK film, resulting in the release of residual surface stress. Additionally, this strategy also optimizes the energy level alignment between the perovskite layer and the HTL, which improves charge extraction and transport. With these cooperative effects, the PCE of Sn-Pb PSCs reaches 21.46% (1 sun, AM1.5), maintaining excellent stability under a nitrogen atmosphere. Hence, the buried interface approach exhibits the potential for achieving high-performance and stable Sn-Pb PSCs.

Tin dioxide (SnO2), one of the best electron transport layer materials for perovskite solar cells (PSCs), has high electrical conductivity and low photocatalytic activity. However, the defects in its inside and surface result in nonradiative recombination at the SnO2/perovskite interface. Complex and time‐consuming passivation methods are not conducive to the commercialization of PSCs, and simple passivation strategies should be used to improve the photovoltaic performance of the devices. Herein, a facile and efficient method is proposed to simultaneously passivate the inside and surface defects by adding histidine (HIS) to SnO2 colloidal solution. This one‐step doping strategy also modulates carrier dynamics at the SnO2/perovskite interface. HIS reduces suspended hydroxyl groups, oxygen vacancies, and uncoordinated Sn4+ defects on the surface of SnO2, as well as uncoordinated Pb2+ and halogen vacancy defects at the buried interface of perovskite. Surprisingly, HIS can prevent perovskite from decomposition to form PbI2, which further decomposes to photoactive metallic Pb0 and I, causing ion migration in PSC. As a result, the PSC efficiency has significantly improved 23.11% after HIS doping. The efficiency of unencapsulated device with HIS is 94% of the primary efficiency after storage in relative humidity = 70 ± 5% for 1000 h.

Deciphering competitive interactions: Phosphate and organic matter binding on goethite through experimental and theoretical insights

The majority of South African healthcare workers are Black Africans with dark-pigmented skin. Studies on how the markers of skin barrier function and natural moisturising factor (NMF) compare between dark and light-pigmented skin are limited. Quantifying NMF in a nursing student population during their practical training at university may provide valuable insight into their potential susceptibility to skin conditions associated with low NMF. The objectives of this study were to quantify and compare NMF content of Black African, Mixed Race and White nursing students from their dominant dorsal hand. Forty-nine White, 32 Black African and 5 Mixed Race nursing students participated in this study. Tape strip samples were collected from the participants' dominant dorsal hand and NMF content was measured, including histidine (HIS), pyrrolidone carboxylic acid (PCA), trans-urocanic acid (t-UCA) and cis-urocanic acid (c-UCA), as well as cytokines interleukin-1 alpha (IL-1α) and interleukin-1 receptor antagonist (IL-1RA). No statistically significant differences in PCA, t-UCA, c-UCA, IL-1α or IL-1RA were found between Black African and White nursing students. HIS was significantly (p = 0.001) higher in White nursing students when compared to Black African students. The ratio of tot-UCA/HIS was significantly higher in Black Africans (p = 0.0002) when compared to White nursing students. No significant differences were established in NMF content between White and Black African nursing students, other than HIS which was significantly higher in White students than in Black African students. Different HIS levels between the racial groups suggest variation in histidase activity which may be related to skin pH and pigmentation. This finding may suggest that nursing students at the beginning of their careers may have similar susceptibility to skin diseases related to NMF.

Density functional theory (DFT) calculations were performed to examine the potential of the RuC nanosheet as a biosensor towards the aromatic amino acids (AAA; tryptophan (TRP), histidine (HIS), tyrosine (TYR), and phenylalanine (PHE)). The AAA molecules were placed vertically and horizontally with respect to the RuC surface and then subjected to geometrical relaxation. According to the geometry relaxation results, it was found that all AAA molecules preferred to be adsorbed on the RuC surface in a horizontal configuration rather than a vertical one, except the HIS molecule, which desired to be vertically adsorbed on the RuC nanosheet. From the energy manifestations, the adsorption process within the TRP⋯RuC complexes had the greatest desired negative adsorption energy (E ads), followed by HIS⋯, TYR⋯, and then PHE⋯RuC complexes (E ads = -40.22, -36.54, -23.95, and -16.62 kcal mol-1, respectively). As indicated by the FMO data, changes in the E HOMO, E LUMO, and E gap values of the RuC nanosheet following the adsorption process demonstrated the capacity of the RuC nanosheet to adsorb the AAA molecules. The outcomes of Bader charge transfer revealed that the RuC nanosheet had the ability to donate electrons to the AAA molecules during the adsorption process, supported by the positive Q t values. Consistent with the E ads conclusions, the TRP⋯RuC complexes had the largest Q t values, indicating the potential affinity of the RuC nanosheet to adsorb the TRP molecule. Following the adsorption of AAA molecules on the RuC nanosheet, new peaks and bands were discovered based on the DOS and the band structure plots, respectively, revealing the validity of the adsorption process. Additionally, the current adsorption findings on the RuC nanosheet were compared to those on the graphene (GN) nanosheet. The outcomes of the comparison demonstrated the outperformance of the RuC nanosheet over the GN nanosheet in adsorbing the AAA molecules. These outcomes provide a solid foundation for further research on the RuC nanosheets to detect small biomolecules.

Introduction: Histamine (HM), a toxic compound, is produced due to an enzymatic reaction when histidine (HS)-rich red fish such as tunas and other fish and their processed products are improperly stored, e.g., leaving them at room temperature. With the increasing popularity of raw fish consumption, HM analysis is mandatory for these fish in various countries. However, the conventional immunoassay method is time-consuming, costly, and technically demanding, burdening wholesalers heavily.Molecularly Imprinted Polymers-based materials are attractive materials for sensor fabrication due to their high selectivity, stability, and ease of production. In this study, we attempted to develop a Molecularly Imprinted Polymer grafted Carbon Paste (MIP-CP) for histamine sensing. Experimental Methods First, a radical polymerization initiator was fixed on the surface of graphite particles [1]. Next, histamine dihydrochloride, itaconic acid, and ethylene glycol dimethacrylate (EDMA) were dissolved in a mixture of distilled water and dimethylformamide to prepare a polymerization solution. The polymerization solution and initiator-fixed graphite were placed in a quartz tube and irradiated with a xenon lamp for 1 hr. HM was extracted and removed using acetic acid. MIP-CP was prepared by mixing MIP-grafted graphite and silicon oil containing ferrocene in a 7:3 weight ratio. Non-imprinted polymer (NIP)-CP was also prepared using the same procedure, except that HM was not added.Differential pulse voltammetry (DPV) was performed with electrodes packed with the obtained MIP-CPs on a disposable chip [2]. The performance of the HM sensor was evaluated based on the relationship between the oxidation current of ferrocene and HM concentration. (Measurement range 0 μg/mL~100 μg/mL) Experimental results The dependence of the oxidation current density on HM concentration at the NIP and MIP electrodes was confirmed (0.0085 μA-mL/μg, R²=0.9357). The increase in current density with increasing HM concentration at the MIP-CP electrode was more than three times greater than at the NIP-CP electrode. This confirmed that MIP-CP exhibited current changes corresponding to the interaction between HM and HM-imprinted sites. Furthermore, the current increased linearly with increasing HM concentration from 0-100 µg/mL. This dynamic range includes 50 µg/mL, which is considered the lower limit of acceptable Hm concentration in fish meat.However, the current value was constant for HS concentration at 0.75 µA over the range of 40-100 µg/mL and below. Therefore, the sensor is saturated with HS at concentrations above 40 µg/mL, and histidine interference can be excluded by subtracting 0.75 µA from the current value obtained when measuring the fish meat sample. Conclusion We successfully developed a reagentless, disposable histamine sensor using MIP-CP, which provides a rapid, reliable, and cost-effective method for histamine detection in fish.

New polymeric macroporous materials based on poly 2-hydroxyethyl methacrylate (pHEMA) were synthesized and tested to adsorb CO2. To this purpose, bio and affordable amine-based molecules such as lysine (LYS) and histidine (HIS) were selected as CO2 active sites and used to functionalize HEMA monomer before its crosslinking polymerization. The as-prepared monomers and polymers were characterized by using Nuclear Magnetic Resonance (NMR), Fourier Infrared Spectroscopy (FT-IR), Thermal gravimetric analysis (TGA), and Scanning Electron Microscopy (SEM) equipped with Energy Dispersive X-ray (EDX). Compared to materials reported in the recent literature, all produced ones provide exceptional adsorption capacity in the 162–193 ppm range. In particular, H-HEMA-LYS exhibits the best adsorption grade, well-fitting the Linear Driving Force (LFD) model. H-HEMA-LYS reusability was also tested for up to 5 cycles without significant loss in capture performance. Finally, to get insight into the role of morphology in CO2 adsorption, two diverse macroporous structures were synthesized (hydrogels and cryogels) for both HIS and LYS-based materials. As it turns out, hydrogel formulations of an average area ranging from 15.5 to 230 μm2 adsorb 12% more than cryogels with higher values (266–605 μm2).

One of the solubilization of poorly water-soluble drugs is the use of cyclodextrin (CD)-based inclusion complexes. On the other hand, few studies have investigated how CD functions on the solubility of drugs in the presence of multiple drugs that interact with each other. In this study, we used indomethacin (IND) and diclofenac (DIC) as acidic drugs, famotidine (FAM) and cimetidine (CIM) as basic drugs, and imidazole (IMZ), histidine (HIS), and arginine (ARG) as compounds structurally similar to basic drugs. We attempted to clarify the effect of β-CD on the solubility change of each drug in the presence of multiple drugs. IND and DIC formed a eutectic mixture in the presence of CIM, IMZ, and ARG, which greatly increased the intrinsic solubility of the drugs as well as their affinity for β-CD. Furthermore, the addition of high concentrations of β-CD to the DIC-FAM combination, which causes a decrease in solubility due to the interaction, improved the solubility of FAM, which was decreased in the presence of DIC. These results indicate that β-CD synergistically improves the solubility of drugs in drug-drug combinations, where the solubility is improved, whereas it effectively improves the dissolution rate of drugs in situations where the solubility is reduced by drug-drug interactions, such as FAM-DIC. This indicates that β-CD can be used to improve the physicochemical properties of drugs, even when they are administered in combination with drugs that interact with each other.

An increasing number of studies indicate that cancer patients' histidine (HIS) circulating levels have changed. However, the causality between HIS and cancer is still not well established. Thus, to ascertain the causal link between HIS and cancers, we performed a bidirectional Mendelian randomization (MR) analysis. Summary-level data are derived from publicly available genome-wide association studies (GWAS). The causal effects were mainly estimated using the inverse-variance weighted method (IVW). The weighted-median (WM) method and MR-Egger regression were conducted as sensitivity analyses. In the forward-MR, we found malignant neoplasm of respiratory system and intrathoracic organs (OR: 1.020; 95% CI: 1.006-1.035; pIVW = 0.007) genetically associated with circulating HIS. And there was no significant genetic correlation between HIS and another 11 site-specific cancers using IVW method. In the reversed-MR, we did not observe the causal relationship between HIS and 12 site-specific cancers. Our findings help clarify that HIS, as a biomarker for malignant neoplasms of respiratory system and intrathoracic organs, is causal rather than a secondary biomarker of the cancerous progression. The mechanism between histidine and cancer progression deserves further investigation.

The zinc/iron-regulated transporter-like protein (ZIP) gene family first identified in plants is highly distributed in the plant kingdom. This family has previously been reported to transport several essential and non-essential cationic elements, including those toxic to many economically important crops such as cacao (Theobroma cacao L.). In this article, we present a detailed study on physicochemical properties, evolution, duplication, gene structure, promoter region and TcZIP family three-dimensional protein structure. A total of 11 TcZIP genes have been identified to encode proteins from 309 to 435 aa, with localization in the plasma membrane and chloroplast, containing 6-9 putative domains (TM). Interspecies phylogenetic analysis subdivided the ZIP proteins into four groups. Segmental duplication events significantly contributed to the expansion of TcZIP genes. These genes underwent high pressure of purifying selection. The three-dimensional structure of the proteins showed that α helix conformations are predominant with several pocket sites, containing the metal binding site, with the residues leucine (LEU), alanine (ALA), glycine (GLY), serine (SER), lysine (LYS) and histidine (HIS) the most predicted. Regarding the analysis of the protein-protein interaction and enrichment of the gene ontology, four biological processes were assigned, the most important being the cation transport. These new discoveries expand the knowledge about the function, evolution, protein structures and interaction of ZIP family proteins in cacao and contribute to develop cacao genotypes enriched with important mineral nutrients as well as genotypes that bioaccumulate or exclude toxic metals.

This study investigated different amino acid-based surfactants (AASs), also known as biosurfactants, including sodium N-dodecyl asparagine (AS), sodium N-dodecyl tryptophan (TS), and sodium N-dodecyl histidine (HS) for their potential anticorrosion, antibacterial, and antidermatophyte properties. The chemical and electrochemical techniques were employed to examine the copper corrosion inhibition efficacy in H2SO4 (1.0 M) solution at 298 K. The results indicated their promising corrosion inhibition efficiencies (% IEs), which varied with the biosurfactant structures and concentrations, and the concentrations of corrosive medium. Higher % IEs values were attributed to the surfactant adsorption on the copper surface and the production of a protective film. The adsorption was in agreement with Langmuir adsorption isotherm. The kinetics and mechanisms of copper corrosion and its inhibition by the examined AASs were illuminated. The surfactants behaved as mixed-kind inhibitors with minor anodic priority. The values of % IEs gained from weight loss technique at a 500 ppm of the tested surfactants were set to be 81, 83 and 88 for AS, HS and TS, respectively. The values of % IEs acquired from all the applied techniques were almost consistent which were increased in the order: TS > HS ≥ AS, establishing the validity of this study. These surfactants also exhibited strong broad-spectrum activities against pathogenic Gram-negative and Gram-positive bacteria and dermatophytes. HS exhibited the highest antimicrobial activity followed by TS, and AS. The sensitivity of pathogenic bacteria varied against tested AASs. Shigella dysenteriae and Trichophyton mantigrophytes were found to be the most sensitive pathogens. HS exhibited the highest antibacterial activity against Shigella dysenteriae, Bacillus cereus, E. coli, K. pneumoniae, and S. aureus through the formation of clear zones of 70, 50, 40, 39, and 35 mm diameters, respectively.AASs also exhibited strong antifungal activity against all the tested dermatophyte molds and fungi. HS caused the inhibition zones of 62, 57, 56, 48, and 36 mm diameters against Trichophyton mantigrophytes, Trichophyton rubrum, Candida albicans, Trichosporon cataneum, and Cryptococcus neoformans, respectively. AASs minimal lethal concentrations ranged between 16 to 128 µg/ml. HS presented the lowest value (16 µg/ml) against tested pathogens followed by TS (64 µg/ml), and AS (128 µg/ml). Therefore, AASs, especially HS, could serve as an effective alternative antimicrobial agent against food-borne pathogenic bacteria and skin infections-associated dermatophyte fungi.